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Optimization of the Protocol for the in VitroCultivation of Piper aduncumL.

American Journal of Plant Sciences 2014年第23期5卷 3474-3482页

作者： Samia Torres Silva fernanda ventorim pacheco Ivan Caldeira Almeida Alvarenga José Eduardo Brasil Pereira Pinto Suzan Kelly Vilela Bertolucci Cibelly Pereira Ferreira Department of Agriculture Federal University of Lavras Lavras Brazil

Piper aduncum L. (Piperaceae) has great potential for economic exploration because of the proven use of its essential oil in the agriculture and in the human health. A technique that improves its propagation, as the tissue culture, becomes necessary. Some parameters must be determined for the successful cultivation in vitro. Thus, this study aimed to determine the salts concentration of MS medium, temperature, luminosity and light quality for in vitro culture of this species. The following treatments were conducted: 1/4MS, 1/2MS, MS and 2MS;20℃, 25℃, 30℃ and 35℃;monochromatic blue, red and white lights and the combination of red and blue, using light emitting diodes (LEDs);luminosities of 17, 37, 48 and 73 μmol·m-2·s-1 obtained with cool white fluorescent lamp. After 35 days, the treatments were analyzed. To determine the salts concentration of MS, the variables were evaluated: number of shoots, leaves and roots, length and width of leaves, length and dry mass of root and shoots and percentage of death by necrosis. To the plantlets under different temperatures, length and dry mass of shoot and root, number of shoots, number, length and width of leaves and survival and rooting percentages were measured. The plantlets that were maintained under different luminosities were evaluated for length of shoot and root, dry mass of leaf and root. To evaluate the growth under the LEDs, the length and dry mass of shoot and root, number of shoots and roots, percentage of rooting and sprouting were assessed. The medium 1/4MS and the medium 1/2MS showed better responses for number and length of root, leaf width and shoot length. The temperature 25℃ provided the highest number of leaves, length of shoot and root, root dry mass and rooting percentage. The luminosity 73 μmol·m-2·s-1 provided higher values in length of root and dry mass of leaf and root. The red LED provided plantlets with greater growth. Thus, for the in vitro cultivation of Piper aduncum, 1/4MS, environment temperatu

关键词： Tissue Culture LEDs Monkey Pepper

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Gas Exchange and Production of Photosynthetic Pigments of Piper aduncumL. Grown at Different Irradiances

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American Journal of Plant Sciences 2013年第12期4卷 114-121页

作者： fernanda ventorim pacheco Helbert Rezende de Oliveira Silveira Amauri Alves Alvarenga Ivan Caldeira Almeida Alvarenga José Eduardo Brasil Pereira Pinto Jean Marcel Sousa Lira Departamento de Agricultura Geral UFLA-Universidade Federal de Lavras Lavras Brazil Setor de Fisiologia Vegetal Departamento de Biologia UFLA-Universidade Federal de Lavras Lavras Brasil

The species Piper aduncum is a plant with great economic potential, because the essential oil has antimicrobial activity and insecticides. Thus, as the light directly affects photosynthesis process, the present study evaluated the gas exchanges’ variables and photosynthetic pigments’ production of P. aduncum grown under different irradiances. Treatments consisted in environments with 100%, 70% and 50% of irradiance and environments with colored nets (red and blue). After 150 days of culture, photosynthetic activity (A), stomatal conductance (gs) transpiration rate (E), internal CO2 concentration (Ci), deficit vapor pressure (DVP), leaf temperature, concentration of internal/external CO2 (Ci/Ca), carboxylation efficiency (A/Ci) and efficiency water use (A/E) were evaluated. Pigments chlorophyll a, b, carotenoids, total and chlorophyll a/b ratio were also measured. From the observed data, it can be concluded that the species Piper aduncun developed various mechanisms for adaptation to different irradiance conditions. Moreover, it can be inferred that their photosynthetic process is more efficient and has larger spectral intensities of light, with higher efficiency photosynthetic when grown under 70% and 100% of radiation.

关键词： Long Pepper Color Nets Chlorophyll Luminosity Ecoambients

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Genoprotection and metabolic benefits of marine macroalgae-Insights into the concept of functional foods through direct and indirect consumption

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Food Bioscience 2022年第3期47卷 524-533页

作者： Ana Marques João Ferreira Ana Cerqueda-pacheco Vitória Pereira Helena Abreu Rui Pereira Maria João Pires fernanda Seixas Paula Oliveira Isabel Gaivão Mário pacheco Laboratory of Physiotoxicology CESAM-Centre for Environmental and Marine StudiesDepartment of BiologyUniversity of AveiroCampus Universitário de Santiago3810-193AveiroPortugal CECAV-Animal and Veterinary Research Center AL4AnimalsUniversity of Trás-os-Montes and Alto DouroQuinta de Prados5000-801Vila RealPortugal Department of Genetics and Biotechnology University of Trás-os-Montes and Alto DouroQuinta de Prados5000-801Vila RealPortugal Department of Biology and Environment University of Trás-os-Montes and Alto DouroQuinta de Prados5000-801Vila RealPortugal CITAB-Centre for the Research and Technology of Agro-Environmental and Biological Sciences Inov4AgroUniversity of Trás-os-Montes and Alto DouroQuinta de Prados5000-801Vila RealPortugal Department of Veterinary Sciences University of Trás-os-Montes and Alto DouroQuinta de Prados5000-801Vila RealPortugal ALGAplus Lda.Travessa Alexandre da Conceição s/n3830-196ÍlhavoPortugal

The consumption of marine macroalgae has been linked with health benefits,albeit some actions,including genoprotection,remain ***,we evaluated the genoprotective potential of a mixture of Ulva rigida,Fucus vesiculosus and Gracilaria gracilis,through direct consumption or via indirect intake using fish(Sparus aurata)as a vector of algal ***(Mus musculus)were subjected to a 1-month dietary supplementation with 5%of macroalgae mix or 10%of fillet of fish previously fed with an algae-supplemented aquafeed(for comparison purposes,10%supplementation with fillet of fish standardly fed was also considered).Thereafter,mice were challenged by the genotoxicant methyl methanesulfonate(MMS),and the genetic damage was evaluated through the micronuclei ***,a wider evaluation was carried out encompassing the assessment of energy metabolism,haematological and histological parameters,as well as growth *** unequivocally protected against MMS-induced *** activity profile of hepatic lactate and isocitrate dehydrogenases promoted by the supplemented diets reflected an improved energy *** properties were not transferred via fish fed with the algae-supplemented aquafeed,though the transference of phytocomponents with beneficial properties(*** balance improvement)should not be ***,these findings highlighted the genoprotection afforded by marine macroalgae,likely promoted by desmutagenic factors,reinforcing their definition as functional *** possibility of using fish as an indirect source of algae-borne phytocomponents proved to be plausible and worthy of further investigations.

关键词： Antigenotoxicity Desmutagens Fish consumption Functional food Marine macroalgae consumption Metabolic benefits

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Active tuberculosis in inflammatory bowel disease patients under treatment from an endemic area in Latin America

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World Journal of Gastroenterology 2020年第44期26卷 6993-7004页

作者： Flora Maria Lorenzo Fortes Ney Boa Sorte Victor D Mariano Laila D Andrade fernanda A Oliveira Monique CA Santos Claudia Ivanilda N dos Santos Catharina A Passos Mila P pacheco Valdiana C Surlo Neogelia P de Almeida Jaciane AM Fontes Andrea M Pimentel Raquel Rocha Genoile Oliveira Santana Pharmaceutical Sciences Pos-graduation Program State University of BahiaSalvadorBA 40460-120Brazil Outpatient Gastroenterology Unit General Hospital Roberto SantosSalvadorBA 40286-901Brazil Life Sciences Department State University of BahiaSalvadorBA 41150-000Brazil Department of Medicine FTC UniversitySalvadorBA 41741-590Brazil Department of Sciences of Nutrition School of NutritionFederal University of BahiaSalvadorBA 41701-035Brazil

BACKGROUND There has been an increase in cases of inflammatory bowel disease(IBD)in recent *** is also greater access and availability of immunosuppressive and biological agents,which increase the risk of opportunistic infection despite improving the quality of life and promoting mucosal ***(TB)remains a public health problem,and it has a high incidence in several ***,knowledge of the risk of developing TB in patients with IBD is *** To evaluate the risk of active TB in patients with IBD under treatment from an endemic area in Latin *** A standard questionnaire included demographic variables,clinical aspects of IBD disease,history of active TB during treatment,active TB characteristics and evolution,initial screening and results and time from the start of anti-tumor necrosis factor alpha(TNFα)to TB *** Azathioprine,anti-TNFα and the combination of these two drugs were associated with a higher risk of active TB *** TNFα blockers increased the relative risk of developing active TB compared to other *** four multivariable models showed that the use of TNFα blockers alone or in combination with azathioprine was an important risk factor for the incidence of active *** adjustment for sex,age,type of IBD and latent TB,anti-TNFα with azathioprine increased the relative risk to 17.8 times more than conventional *** TB,which was diagnosed 3 mo after the start of anti-TNFα,was the most *** Treatment with anti-TNFα increased the risk of active TB in IBD patients from an endemic area in Latin *** risk was increased when anti-TNFα was combined with *** time from the beginning of the treatment to the active TB diagnosis suggests a new TB infection.

关键词： Inflammatory bowel disease Therapy Tumor necrosis factor alpha Relative risk Tuberculosis Latent tuberculosis

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Photobiomodulation therapy for osteoarthritis: Mechanisms of action

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World Journal of Translational Medicine 2022年第3期10卷 29-42页

作者： Fábio Pericinoto Giolo Gabriel Silva Santos Victor Fontes pacheco Stephany Cares Huber Kaue Franco Malange Bruno Lima Rodrigues fernanda Bassora Tomas Mosaner Gabriel Azzini Lucas Leite Ribeiro Carlos Amilcar Parada JoséFábio Santos Duarte Lana Department of Physical Therapy Brazilian Institute of Regenerative MedicineIndaiatuba 13334-170Brazil Biomedical Science Brazilian Institute of Regenerative MedicineIndaiatuba 13334-170Brazil Department of Orthopedics Brazilian Institute of Regenerative MedicineIndaiatuba 13334-170Brazil Neurobiology of Pain and Regenerative Medicine The University of CampinasCampinas 13083-862Brazil Department of Hematology The University of CampinasCampinas 13083-878Brazil

Photobiomodulation(PBM)is a non-invasive therapeutic modality with demonstrated effects in many fields related to regenerative *** the field of orthopedics,in particular,PBM at various wavelengths has demonstrated the capacity to trigger multiple biological effects associated with protective mechanisms in musculoskeletal *** articles cited in this review show that devices operating close to or within the near infrared range at low intensities can provoke responses which favor the shift in the predominant catabolic microenvironment typically seen in degenerative joint diseases,especially osteoarthritis(OA).These responses include proliferation,differentiation and expression of proteins associated with stable cell ***,PBM can also modulate oxidative stress,inflammation and pain by exerting regulatory effects on immune cells and blocking the transmission of pain through sensory neuron fibers,without adverse ***,these effects are essential in order to control the progression of OA,which is in part attributed to exacerbated inflammation and degradative enzymatic reactions which gradually contribute to the destruction of joint *** may offer medical experts ease of application,financial viability,efficacy and lack of serious adverse ***,it may prove to be a suitable ally in the management of mild to moderate degrees of *** review explores and discusses the principal biological mechanisms of PBM and how the produced effects may contribute to the amelioration of osteoarthritic *** was reviewed using PubMed and Google Scholar in order to find studies describing the mechanisms of *** investigation included a combination of nomenclature such as:“photobiomodulation”,“phototherapy”,“laser therapy”,“PBM”,“osteoarthritis”,low level light therapy”,“inflammation”and“cartilage”.We considered only articles written in English,with access to the full text.

关键词： Photobiomodulation Low-level laser therapy Osteoarthritis Inflammation Regenerative medicine

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Adrenal Insufficiency by Adrenoleukodystrophy

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Health 2020年第1期12卷 1-13页

作者： Elda Cristinne Mattos Botelho Eduardo Romero Sampaio Botelho Luciana Correa de Souza Rodrigues Tatiana Prado Wanderley fernanda Navarini Queiroz Pollyana Cirino Gomes Borges Sabrina Arrais Rolim Aragao Ximenes Cruz Janete pacheco da Silva Larissa Bianca Cunha de Sa Alberto Krayyem Arbex IPEMED Medical School AFYA EducationalBrasíliaBrazil Medical Clinic in Endocrinology and Diabetology Schleswig-FlensburgGermany

The X-linked adrenoleukodystrophy (ALD) is a severe neurodegenerative disorder due to mutations in the ABCD1 gene. Objective: To report a case of a 19-year-old man with adrenal insufficiency due to adrenoleukodystrophy. Method: Case report and literature review. Result: A previously healthy 19-year-old male patient was admitted to the emergency room with nausea and vomiting for 5 days, who progressed to abdominal pain, severe asthenia, and fever (38.5°C). He referred progressive darkening of the skin, oral mucosa, tongue and nail bed of the hands and feet, observed in the last 6 years. Emergency laboratory evaluation showed severe hyponatremia and hyperkalemia, which, together with decreased plasma cortisol, directed the investigation to causes of adrenal insufficiency. High ACTH (Adrenocorticotropic hormone) and very long chain fatty acid levels closed the diagnosis. Discussion: ALD is characterized by progressive demyelination in the central and peripheral nervous system and adrenal insufficiency consequence to the accumulation of very long chain fatty acids (VLCFA) in the adrenal. The overall incidence of ALD is 1:17,000. Adrenal insufficiency may be the first symptom of ALD in boys and adults. The diagnosis is based on the measurement of VLCFA plasma levels. Allogeneic bone marrow transplantation is the only treatment that provides a permanent cure when the procedure is performed at an early stage of brain demyelination, i.e. when patients are asymptomatic, although brain magnetic resonance imaging (MRI) is abnormal. Treatment of Addison’s disease is obligatory, but does not change the course of neurological symptoms.

关键词： Adrenoleukodystrophy Adrenomyeloneuropathy Adrenal Insufficiency Addison’s Disease

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DNA Vaccines Approach:From Concepts to Applications

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World Journal of Vaccines 2014年第2期4卷 50-71页

作者： Vanessa Bastos Pereira Meritxell Zurita-Turk Tessalia Diniz Luerce Saraiva Camila Prosperi De Castro Bianca Mendes Souza Pamela Mancha Agresti fernanda Alvarenga Lima Vanessa Nathalie Pfeiffer Marcela Santiago pacheco Azevedo Clarissa Santos Rocha Daniela Santos Pontes Vasco Azevedo Anderson Miyoshi Laboratorio de Genetica Celular e Molecular Instituto de Ciencias BiologicasUniversidade Federal de Minas Gerais(UFMG)Belo HorizonteBrazil Universidade Estadual da Paraiba(UEPB) Joao PessoaBrazil

DNA vaccines are the third generation vaccines based on purified plasmid preparations containing transgenes that encode antigenic/therapeutic proteins or peptides capable of triggering an immune response against a wide range of diseases. This vaccine platform presents several attributes that confer distinct advantages over other vaccine technologies in terms of safety, ease of fabrication and stability. Many aspects, such as antigen expression and especially vector design, are under study because of their great influence on immunogenicity and efficacy of DNA vaccines. In this regard, with the attempt of improving the efficiency of DNA vaccines, co-expression of stimulatory sequences and diverse vector delivery systems are being optimized. With this in mind, this review aims to giving a conceptual approach of DNA vaccines, explaining their mechanisms of action and listing the already licensed veterinary DNA vaccines presented in the market.

关键词： DNA Vaccines Vector Design Delivery Systems Approved DNA Vaccines Bacterial Delivery

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