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Physico-chemical characterization of acid base disorders in patients with COVID-19:A cohort study

World Journal of Nephrology 2024年第2期13卷 16-23页

作者： Sergio Pinto de Souza Juliana R Caldas Marcelo Barreto Lopes Marcelo Augusto Duarte Silveira fernanda oliveira coelho Igor oliveira Queiroz Pedro Domingues Cury Rogério da Hora Passos Department of Nephrology Hospital São RafaelSalvadorBA 41253190Brazil Department of Nephrology D’Or Institute for Research and Education(IDOR)SalvadorBA 41253190Brazil Faculty of Medicine Escola Bahiana de Medicina e Saúde Pública-EBMSPSalvadorBA 40290000Brazil Department of Intensive Care D’Or Institute for Research and Education(IDOR)SalvadorBA 41253190Brazil Hospital São Rafael D’Or Institute for Research and Education(IDOR)SalvadorBA 41253190Brazil Department of Intensive Care Unit Hospital Israelita Albert EinsteinSao PauloSP 05652900Brazil

BACKGROUND Acid-base imbalance has been poorly described in patients with coronavirus disease 2019(COVID-19).Study by the quantitative acid-base approach may be able to account for minor changes in ion distribution that may have been over-looked using traditional acid-base analysis *** a cohort of critically ill COVID-19 patients,we looked for an association between metabolic acidosis surrogates and worse clinical outcomes,such as mortality,renal dialysis,and length of hospital *** To describe the acid-base disorders of critically ill COVID-19 patients using Stewart’s approach,associating its variables with poor *** This study pertained to a retrospective cohort comprised of adult patients who experienced an intensive care unit stay exceeding 4 days and who were diagnosed with severe acute respiratory syndrome coronavirus 2 infection through a positive polymerase chain reaction analysis of a nasal swab and typical pulmonary involvement observed in chest computed tomography *** and clinical data were obtained from electronic *** variables were compared using Fisher’s exact *** data were presented as median and interquartile *** Mann-Whitney U test was used for *** In total,211 patients were *** mortality rate was 13.7%.Overall,149 patients(70.6%)presented with alkalosis,28 patients(13.3%)had acidosis,and the remaining 34 patients(16.2%)had a normal arterial pondus *** those presenting with acidosis,most had a low apparent strong ion difference(SID)(20 patients,9.5%).Within the group with alkalosis,128 patients(61.0%)had respiratory *** non-survivors were older,had more comorbidities,and had higher Charlson’s and simplified acute physiology score *** did not find severe acid-base imbalance in this *** analyzed Stewart’s variables(effective SID,apparent SID,and strong ion gap and the effect of albumin,lactate,phosphorus,and chloride)were not d

关键词： COVID-19 Physicochemical approach Acid-base status Critically ill patients Acute respiratory syndrome

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Mindfulness training in medical education as a means to improve resilience,empathy,and mental health in the medical profession

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World Journal of Psychiatry 2024年第4期14卷 489-493页

作者： Edison Iglesias de oliveira Vidal Luiz Fernando Alvarenga Ribeiro Marco Antonio de Carvalho-Filho fernanda Bono Fukushima Internal Medicine Department Botucatu Medical SchoolSao Paulo State University(UNESP)Botucatu 18618-687SPBrazil Wenckebach Institute for Education and Training LEARN-Lifelong LearningEducation and Assessment Research NetworkUniversity Medical Center GroningenGroningen 9713AVGroningenNetherlands Assertiva Mindfulness Botucatu 18603-970SPBrazil Surgical Specialties and Anesthesiology Department Botucatu Medical SchoolSão Paulo State University(UNESP)Botucatu 18618-687SPBrazil

The high rates of depression,burnout,and increased risk of suicide among medical students,residents,and physicians in comparison with other careers signal a mental health crisis within our *** contend that this crisis coupled with the inadequate acquisition of interpersonal skills during medical education results from the interaction between a challenging environment and the mental capital of ***,we posit that mindfulness-based practices are instrumental for the development of major components of mental capital,such as resilience,flexibility of mind,and learning skills,while also serving as a pathway to enhance empathy,compassion,self-awareness,conflict resolution,and relational ***,the evidence base supporting the effectiveness of mindfulness-based interventions has been increasing over the years,and a growing number of medical schools have already integrated mindfulness into their *** we acknowledge that mindfulness is not a panacea for all educational and mental health problems in this field,we argue that there is currently an unprecedented opportunity to gather momentum,spread and study mindfulness-based programs in medical schools around the world as a way to address some longstanding shortcomings of the medical profession and the health and educational systems upon which it is rooted.

关键词： Mindfulness Medical education Mental capital Mental health Medical students Resilience

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Influence of biological maturation on cardiac autonomic recovery in female volleyball players during&after repeated sprints training:An experimental trial

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Sports Medicine and Health Science 2024年第3期6卷 279-286页

作者： Paulo Francisco de Almeida-Neto fernanda Cristina Silva de oliveira Jose Marcondes de oliveira-Júnior Júlio Cesar Medeiros Alves Matheus de Lima Rocha Iago Medeiros da Silva Roberto Felipe C^amara Rocha Paulo Moreira Silva Dantas Breno Guilherme de Araújo Tin^oco Cabral Health Sciences Center Federal University of Rio Grande do NorteCCS-UFRNNatal59012-570RNBrazil Department of Physical Education Federal University of Rio Grande do NorteDEF-UFRNNatal59078-970RNBrazil

Previously,it was suggested that biological maturation(BM)could be linked to cardiac autonomic recovery(CAR)in the pediatric ***,this influence hasn’t been confirmed *** aim was to investigate the impact of BM on CAR in female volleyball *** study with a sample of 38 volleyball players,comprising 20 girls(age:[11.62.1]years)and 18 women(age:[24.55.5]years),we analyzed BM,comparing maturing subjects(girls)with mature subjects(women).Additionally,we assessed peak height velocity(PHV)in *** conducted a training session involving repeated sprints(3 rounds of 6 sprints interspersed by 5 min[min]of passive rest).Using short-range radio telemetry,we analyzed CAR during(at the end of the 1st and 2nd rounds)and after(following the 3rd round)the training session of repeated sprints by applying the 60-s to 300-s heart rate recovery index(HRR-Index).Girls exhibited superior CAR compared to women(round 2:60-s,120-s,240-s,and 300-s,p0.4,p0.4,p<0.05).We have concluded that biological maturation has a significant impact on cardiac autonomic recovery.

关键词： Exercise Sport Volleyball Heart rate Pediatric

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Risk Stratification in Obstetrics: An Integrated Approach to Maternal Health

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Open Journal of Obstetrics and Gynecology 2024年第6期14卷 903-910页

作者： Taina Crespo Mendonca Bianca Beatriz Soares dos Reis Júlia Cruz coelho Antônio de oliveira Battistini Pestana Vitória Carolina Barreto Negri Alvaro Augusto Trigo Department of Medicine University of Franca Franca Brazil

Introduction: Prenatal care is crucial for maternal and fetal health, highlighting the importance of obstetric risk stratification. This enables personalized care, avoiding unnecessary interventions and reducing costs. Conditions such as first trimester bleeding, spontaneous abortion, gestational trophoblastic disease, and ectopic pregnancy require specific approaches. Early identification of these complications is vital, especially in urgent and emergency obstetric cases, which demand immediate hospital attention. Objective: Comprehensive review of first trimester pathologies and gestational hypertension, focusing on obstetric risks and personalized prenatal management. Methods: Literature review on bleeding and gestational hypertension. Results: The study provides a detailed analysis of pathologies associated with first trimester uterine bleeding and hypertensive disorders, focusing on obstetric risk stratification. The main causes of maternal and fetal mortality are hypertensive disorders, hemorrhages, infections, childbirth complications, and abortion. Spontaneous abortion is common, with different classifications, ranging from threatened to missed abortion. Infected abortion is a severe complication. Brazil has restrictive abortion laws, but many unsafe abortions occur, resulting in high public health costs. Pharmacological treatment with misoprostol is a safe option. Other pathologies include gestational trophoblastic disease, ectopic pregnancy, and placenta previa. Preeclampsia and eclampsia are severe conditions requiring immediate treatment to avoid complications. Conclusion: Early identification and management of obstetric risk factors, such as uterine bleeding, are essential for positive maternal and fetal outcomes. A multidisciplinary approach is fundamental.

关键词： Obstetrics Emergencies Hypertension Pregnancy-Induced Uterine Hemorrhage Pregnancy Trimester First

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Feasibility of performing the 3-minute step test with remote supervision in children and adolescents with cystic fibrosis: A comparative study

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Pediatric Investigation 2024年第2期8卷 83-90页

作者： fernanda Maria Vendrusculo Gisele Apolinario da Costa Maria Amelia Bagatini Brenda Maria Henrique Maia Lemes Carolina Aguiar Faria Larissa Carvalhaes de oliveira Evanirso da Silva Aquino Marcio Vinicius Fagundes Donadio Laboratory of Pediatric Physical Activity Infant CenterPontificia Universidade Catolica do Rio Grande do Sul(PUCRS)Porto AlegreBrazil Hospital Infantil Joao Paulo II-Fundacao Hospitalar do Estado de Minas Gerais(FHEMIG) Belo HorizonteBrazil Departament of Physiotherapy Pontificia Universidade Catolica de Minas Gerais(PUCMG)-Campus BetimBetimBrazil Department of Physiotherapy Faculty of Medicine and Health SciencesUniversitat Internacional de Catalunya(UIC)BarcelonaSpain

Importance:The 3-min step test is a simple option to monitor submaximal exercise capacity,although its use via remote video monitoring has not been investigated in children with cystic fibrosis(CF).Objective:This study aimed to assess the feasibility and reproducibility of performing the 3-min step test with remote ***:A cross-sectional study including CF patients(6-18 years)from two CF services were ***,anthropometric,clinical,and lung function data were collected and two 3-min step tests were performed:(i)in-person supervision,and(ii)remotely supervised by video *** and after the tests,heart rate(HR),oxygen saturation(SpO_(2)),and the Borg score for dyspnea and lower limb fatigue were ***:Twenty-three patients(10.7±3.7 years)with a mean FEV1 of 89.5%±23.2%were *** were no significant differences between tests,with mean differences(95%confidence intervals)in final HR of-3.3(-8.9,2.4),change in HR of-1.9(-6.1,2.1),final SpO_(2) of 0.3(-0.4,1.0),and final dyspnea of 0.1(-0.8,0.9).The intraclass correlation coefficient was 0.852(final HR),0.762(final SpO_(2)),and 0.775(final lower limb fatigue).Significant and moderate correlations were found between tests for final HR(r=0.75),change in HR(r=0.61),and final SpO_(2)(r=0.61).The Bland-Altman analysis showed a mean difference in final SpO_(2) between tests of 0.3%(limit of agreement-3.0%,3.5%).Interpretation:Physiological responses between tests were similar,indicating it was feasible to perform the 3-min step test with remote supervision in CF children.

关键词： Children Cystic fibrosis Exercise test Telehealth

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Decontamination of mobile phones and electronic devices for health care professionals using a chlorhexidine/carbomer 940■gel

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Frontiers of Chemical Science and Engineering 2019年第1期13卷 192-198页

作者： Rafael Muniz de oliveira Nereida Mello da Rosa Gioppo Jancineide oliveirade Carvalho Francilio Carvalho oliveira Thomas Jay Webster fernanda Roberta Marciano Anderson oliveira Lobo University Hospital of the West of Parana State University of West of Parana Cascavel PR Brazil University Center for Health. Humanities and Technology of Piaui (UNINOVAFAPI). Piaui Brazil Science and Technology Institute Brasil University Itaquera SP Brazil Nanomedicine Laboratory Department of Chemical Engineering Northeastern University MA 02115 USA Materials Science and Engineering graduation program Technology Center Federal University of Piaui. PI Brazil

Though they reduce microorganism growth, current hospital disinfectants also damage many of today’s modern electronic devices such as tablets and smartphones. Herein, the efficacy of a new chlorhexidine digluconate gel (CDG) was tested as a disinfectant for mobile and electronic devices in a clinical environment. Specifically, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa and methicillin resistant Staphylococcus aureus were used to infect the screen of eight smartphones. The CDG was prepared at concentrations of 2%, 4% and 6%, and tested on paper disks infected with these bacteria before being tested on the smartphones. The devices were disinfected with the CDG gel (4%) at two times: immediately and after 5 min of the bacterial contamination. In all cases, the CDG gel eliminated 100% of gram-positive and gram-negative microorganisms compared to the control (without any agent). In addition, the gel did not damage the smartphones. Therefore, our study suggests that the CDG gel may be applied to disinfect a wide range of electronic devices for health care professionals in the hospital environment.

关键词： smartphones disinfection clinical environment chlorhexidine bacteria

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Development of a Ready-to-Use PSMA-11 Kit Formulation and Biological Evaluation of Binding Affinity

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Advances in Molecular Imaging 2024年第2期13卷 7-22页

作者： Maria Renata Valente Brandão Freire Natália Marchesan Bexiga Marco Antonio Stephano João Ezequiel oliveira Carlos Roberto Jorge Soares Ana Cláudia Camargo Miranda Anna Carolina de Alcântara Barbosa Marycel Figols de Barboza Laura fernanda Garcia Luiz Felipe S. Teixeira Maria Helena Marumo Bellini Natanael Gomes da Silva Luiza Mascarenhas Balieiro Joel Mendes Elaine Bortoleti de Araújo Centro de Radiofarmcia Instituto de Pesquisas Energticas e Nucleares (IPEN) Cidade Universitria So Paulo Brasil Departamento de Tecnologia Farmacutica Faculdade de Cincias Farmacuticas (USP) Cidade Universitria So Paulo Brasil Centro de Biotecnologia Instituto de Pesquisas Energticas e Nucleares (IPEN) Cidade Universitria So Paulo Brasil Departamento de Medicina Nuclear do Hospital Israelita Albert Einstein So Paulo/SP Brasil

Introduction: 68Ga-PSMA-11 is considered the gold standard in detection of micro and oligometastases in advanced prostate cancer, being used for therapeutic planning, as well as, potentially, for evaluating response to treatment. The development of ready-to-use lyophilized kit of PSMA-11 adds quality and safety to the routine use of this radiopharmaceutical and represents a pharmacotechnical challenge as it must preserve the integrity and specificity of the ligand. Methods: PSMA-11 kit formulation was proposed, considering radiolabeling parameters and the preservation of the peptide during the lyophilization process, using mannitol as an excipient. Critical temperature characterization studies were carried out using DSC equipment and the freeze-drying process was developed. The direct radiolabeling conditions were evaluated and standardized using 68Ge/68Ga generator eluate from two different manufacturers (ITG and Eckert & Ziegler). The radiochemical purity was evaluated by TLC and HPLC. Biological evaluation was carried out with lyophilized PSMA-11 to demonstrate the integrity of the peptide and preservation of biological activity after the lyophilization process. Results: Based on critical temperature characterization studies, the freeze-drying cycle was designed to reach a freezing temperature of around −40˚C and primary drying at 2˚C. Using 20 mg of mannitol, an intact and elegant lyophilized cake was obtained. PSMA-11 lyophilized kit was directly labeled with 68Ga eluate from 68Ge/68Ga GMP generators (ITG and Eckert & Ziegler) resulting in % RP > 95% at pH 4.0 to 4.5. The results obtained from in vitro and in vivo biological competition studies confirmed the preservation of PSMA-11 affinity for the receptor after lyophilization. Conclusion: A lyophilized formulation (Kit) of PSMA-11 was successfully obtained, which preserved the integrity and biological activity of the peptide and guaranteed radiolabeling efficiency.

关键词： 68Ga-PSMA-11 Prostate-Specific Membrane Antigen (PSMA) Ready-to-Use Kit GMP-Grade Generators Metastatic Prostate Cancer PET Imaging

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Silymarin Attenuates Hepatic and Pancreatic Redox Imbalance Independent of Glycemic Regulation in the Alloxan-induced Diabetic Rat Model

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Biomedical and Environmental Sciences 2020年第9期33卷 690-700页

作者： Miranda Laise Mara oliveira Agostini Livia da Cunha Lima Wanderson Geraldo de Camini fernanda Caetano Costa Daniela Caldeira Programa de Pos-graduagao em Saude e Nutricao Escola de NutricaoUniversidade Federal de Ouro PretoOuro PretoMinas Gerais 35400-000Brazil Escola de Farmacia Universidade Federal de Ouro Preto.Ouro PretoMinas Gerais 35400-000Brazil Laboratorio de Morfopatologia Departamento de Ciencias BioldgicasUniversidade Federal de Ouro PretoOuro Preto 35400-000Brazil Programa de Pos-graduaqao em Ciencias Bioldgicas Nucleo de Pesquisas em Ciencias BiologicasUniversidade Federal de Ouro Preto.Ouro Preto 35400-000Brazil Laboratorio de Bioquimica Metabolica Departamento de Ciencias BiologicasUniversidade Federal de Ouro PretoOuro Preto 35400-000Brazil

Objective To evaluate the efficiency of silymarin(SMN) in modulating metabolic parameters and redox status in rats with type 1 diabetes mellitus(T1 DM).Methods Diabetes was induced by intraperitoneal injection of alloxan. The diabetic rats were administered with SMN at doses of 50 and 100 mg/kg body weight/d for 30 consecutive days. The rats were divided into the following four groups: vehicle control, diabetic(alloxan-treated), DS50(alloxan +50 mg/kg body weight/d of SMN), and DS100(alloxan + 100 mg/kg body weight/d of SMN) groups. The bodyweight and food and water intake were evaluated. After 30 d, the animals were euthanized and the blood was collected for measuring the serum levels of glucose, triacylglycerol(TAG), urea, and creatinine. The liver and pancreas were collected for measuring the activities of superoxide dismutase(SOD) and catalase(CAT), and the levels of carbonylated protein(PC). The pancreas sample was also used for histological *** SMN reduced hepatic(P < 0.001) and pancreatic(P < 0.001) protein damage and creatinine levels(P = 0.0141) in addition to decreasing food(P < 0.001) and water intake(P < 0.001). However,treatment with SMN did not improve beta-cell function or decrease blood glucose levels in diabetic *** SMN improved polyphagia and polydipsia, renal function, and protected the liver and pancreas against protein damage without affecting hyperglycemia in diabetic animals.

关键词： Silymarin Silybum marianum Type 1 diabetes Oxidative stress

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A stability-indicating high performance liquid chromatography method to determine apocynin in nanoparticles

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Journal of Pharmaceutical Analysis 2017年第2期7卷 129-133页

作者： Juliana Kovalczuk de oliveira Débora fernanda Veres Ronik Jociani Ascari Rubiana Mara Mainardes Najeh Maissar Khalil Department of Pharmacy Laboratory of Pharmaceutical NanotechnologyUniversidade Estadual do Centro-OesteRua Simeao Camargo Varelade Sá.85040-080 GuarapuavaBrazil Department of Biological Sciences Universidade Tecnológica Federal do ParanáRua Cerejeiras S/N85892-000 Santa HelenaPRBrazil

In this study, we developed and validated a fast, specific, sensitive, precise and stability-indicating high performance liquid chromatography（HPLC） method to determine the drug apocynin in bovine serum albumin（BSA） nanoparticles. Chromatographic analyses were performed on an RP C18 column and using a photodiode array detector at a wavelength of 276 nm. Mobile phase consisted of a mixture of acetonitrile and 1% acetic acid（60：40, v/v）, and it was eluted isocratically at a flow rate of 0.8 mL/min. The retention time of apocynin chromatographic peak was 1.65 min. The method was linear, precise, accurate and specific in the range of 5–100 μg/mL. The intra-and inter-day precisions presented relative standard deviation（RSD） values lower than 2%. The method was robust regarding changes in mobile phase proportion, but not for flow rate. Limits of detection and quantitation were 78 ng/mL and 238 ng/mL, respectively. Apocynin was exposed to acid and alkali hydrolysis, oxidation and visible light. The drug suffered mild degradation under acid and oxidation conditions and great degradation under alkali conditions. Light exposure did not degrade the drug. The method was successfully applied to determine the encapsulation efficiency of apocynin in BSA nanoparticles.

关键词： Apocynin Nanoparticles Bovine serum albumin HPLC

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Paleoproterozoic juvenile magmatism within the northeastern sector of the Sao Francisco paleocontinent:Insights from the shoshonitic high Ba-Sr Montezuma granitoids

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Geoscience Frontiers 2020年第5期11卷 1821-1840页

作者： Samuel Moreira Bersan Alice fernanda de oliveira Costa Andre Danderfer Francisco Roberio de Abreu Cristiano Lana Glaucia Queiroga Craig Storey Hugo Moreira Universidade Federal de Ouro Preto Programa de Pos-graduacaoDepartamento de GeologiaCampus Morro do CruzeiroOuro PretoMGCEP 35400-000Brazil Universidade Federal de Ouro Preto Departamento de GeologiaCampus Morro do CruzeiroOuro PretoMGCEP 35400-000Brazil Sustain Geologia Ltda Teresa M.Valadares 503/502Belo HorizonteMGCEP 30575-160Brazil University of Portsmouth School of Earth and Environmental SciencesBurnaby BuildingBurnaby RoadPortsmouthPO13QLUK

New,integrated petrographic,mineral chemistry,whole rock geochemical,zircon and titanite UPb geochronology,and zircon Hf isotopic data from the Montezuma granitoids,as well as new geochemical results for its host rocks represented by the Corrego Tingui Complex,provides new insights into the late-to post-collisional evolution of the northeastern Sao Francisco paleocontinent.U-Pb zircon dates from the Montezuma granitoids spread along the Concordia between ca.2.2 Ga to 1.8 Ga and comprise distinct *** I have crystallization ages between ca.2.15 Ga and 2.05 Ga and are interpreted as inherited *** II zircon dates vary from 2.04 Ga to1.9 Ga and corresponds to the crystallization of the Montezuma granitoids,which were constrained at ca.2.03 Ga by the titanite U-Pb *** age zoning is common within the ca.1.8 Ga Group III zircon ages,being related to fluid isotopic re-setting during the Espinhaco rifiting ***εHf(t)analysis show dominantly positive values for both Group I(-4 to+9)andⅡ(-3 to+8)zircons and TDM2 model ages of 2.7-2.1 Ga and 2.5-1.95 Ga,***,the Montezuma granitoids are weakly peraluminous to metaluminous magnesian granitoids,enriched in LILES and LREE,with high to moderate Mg#and depleted in some of the *** lithochemical signature,added to the juvenile signature of both inherited and crystallized zircons,allowed its classification as a shoshonitic high Ba-Sr granitoid related to a late-to post-collisional lithosphere delamination followed by asthenospheric *** this scenario,the partial melting of the lithospheric mantle interacted with the roots of an accreted juvenile intra-oceanic arc,being these hybrid magma interpreted as the source of the Montezuma *** Corrego Tingui Complex host rocks are akin to a syn-to late-collisional volcanic arc granitoids originated from the partial melting of ancient crustal *** results presented in this study have revealed the occurrence of j

关键词： Zircon U–Pb-Hf Titanite U–Pb High Ba–Sr Late-to post-collisional Sao Francisco paleocontinent

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