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方竹笋膳食纤维作为益生元对乳酸菌生长的影响

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食品与发酵工业 2022年第6期48卷 174-180页

作者：赵泓舟杜木英 ZALAN Zsolt hegyi ferenc 吴良如张同芳郑炯西南大学食品科学学院重庆400715 匈牙利农业与生命科学大学食品科学与技术研究所布达佩斯1011-1239 国家林业和草原局竹子研究开发中心浙江杭州310012

为了探究竹笋膳食纤维(bamboo shoot dietary fiber,BSDF)作为益生元对乳酸菌生长的影响,以常见益生元菊粉为对照,检测了BSDF在模拟胃肠液中的抗消化性,以及对乳酸菌L1(Lactobacillus fermentum)、L2(Lactobacillus plantaum)生长过程... 详细信息

为了探究竹笋膳食纤维(bamboo shoot dietary fiber,BSDF)作为益生元对乳酸菌生长的影响,以常见益生元菊粉为对照,检测了BSDF在模拟胃肠液中的抗消化性,以及对乳酸菌L1(Lactobacillus fermentum)、L2(Lactobacillus plantaum)生长过程中pH值、总酸含量和生长曲线的影响。结果表明,BSDF中总膳食纤维、不溶性膳食纤维、可溶性膳食纤维含量分别为64.90%、58.12%、6.78%,相对含量较高的单糖有葡萄糖(39.72%)、阿拉伯糖(12.77%)、木糖(12.69%)。BSDF在胃肠液中具有一定的抗消化性,模拟消化6 h后,BSDF在人工胃液(pH=3.5)和人工肠液(pH=7)的水解率分别为15.54%、31.08%,均低于菊粉对照组。对于乳酸菌L1,随着BSDF添加量的增加,培养基中的菌群浓度不断增加,BSDF相比于菊粉更有利于乳酸菌L1生长。对于乳酸菌L2,随着BSDF添加量的增加,总酸含量逐渐增大,乳酸菌的益生效应也随之增强。该研究可为BSDF作为益生元的开发利用提供理论参考。

关键词：竹笋膳食纤维乳酸菌抗消化实验益生效应生长曲线

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荧光假单胞菌ZX对葡萄采后灰霉病的防治

食品工业科技 2021年第22期42卷 125-132页

作者：魏雪江孟遥钟涛张曼王智荣 ZSOLT Zalan ferenc hegyi KRISZTINA Takacs 杜木英西南大学食品科学学院重庆400715 中匈食品科学联合研究中心重庆400715 匈牙利国家农业创新研究中心匈牙利布达佩斯1022 农业部农产品贮藏保鲜质量安全风险评估实验室(重庆) 重庆400715

研究荧光假单胞菌(Pseudomonas fluorescens)ZX对葡萄采后灰霉病的防治效果。以“巨峰”葡萄为试材,测定*** ZX的控病效果和生长动态,在离体条件下通过孢子萌发观察、挥发性物质抑菌测试、扫描电镜探究其对灰葡萄孢霉(Botrytis cinerea... 详细信息

研究荧光假单胞菌(Pseudomonas fluorescens)ZX对葡萄采后灰霉病的防治效果。以“巨峰”葡萄为试材,测定*** ZX的控病效果和生长动态,在离体条件下通过孢子萌发观察、挥发性物质抑菌测试、扫描电镜探究其对灰葡萄孢霉(Botrytis cinerea)的防治。结果表明,使用*** ZX处理,葡萄果实的发病率仅为24.8%,明显低于对照组(发病率64.67%),病斑直径最小,可以明显抑制***孢子萌发、芽管伸长。*** ZX产生的挥发性物质具有较强的抑菌效果,并且能对***有重寄生作用,破坏菌丝的正常形态。另外,无论是浸泡还是接种处理,*** ZX都能在葡萄果实上快速地定殖生长。由此表明,*** ZX通过抑制***生长来控制葡萄采后灰霉病,为果实采后防腐保鲜提供一种新的选择。

关键词：荧光假单胞菌生物防治葡萄采后病害

荧光假单胞菌ZX对采后锦橙绿霉病的防治及其抑菌机制

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微生物学报 2019年第5期59卷 950-964页

作者：王智荣梅小飞杜木英江孟遥张洪新汪开拓 Zsolt Zalán ferenc hegyi 阚建全西南大学食品科学学院重庆400715 中匈食品科学联合研究中心重庆400715 农业部农产品贮藏保鲜质量安全风险评估实验室(重庆) 重庆400715 重庆三峡学院生命科学与工程学院重庆404100 匈牙利国家农业研究和创新中心食品科学研究所

【目的】采后柑橘极易受指状青霉(Penicillium digitatum)侵染而发生严重的绿霉病腐烂,生物防治因具有安全、有效、环保等特点近年来备受关注。论文旨在研究荧光假单胞菌(Pseudomonas fluorescens)ZX对采后柑橘绿霉病的防治效果,揭示***... 详细信息

【目的】采后柑橘极易受指状青霉(Penicillium digitatum)侵染而发生严重的绿霉病腐烂,生物防治因具有安全、有效、环保等特点近年来备受关注。论文旨在研究荧光假单胞菌(Pseudomonas fluorescens)ZX对采后柑橘绿霉病的防治效果,揭示***对***可能存在的作用机制。【方法】以"北碚447"锦橙果实为试材,先分别接种20μL拮抗菌培养液、滤液(培养液离心后,上清经0.22μm滤膜过滤)、菌悬液(培养液离心后,菌体用无菌水反复洗涤并用无菌水重悬)和热杀死液(培养液高温高压灭菌),2 h后接种20μL P. digitatum孢子悬浮液(1×10~4spores/m L),所有果实于20oC、90%相对湿度环境下恒温恒湿培养8 d后,测定果实的发病率和病斑直径;制备柑橘皮培养基,进行平板抑菌试验,探索P. fluorescens ZX对P. digitatum孢子发芽情况的影响;采用两板对扣法和生物熏蒸法研究***挥发性次级代谢产物的抑菌作用;利用插入式细胞培养皿等分析***和***之间竞争的营养物质;同时,测定***的生长曲线,利用结晶紫染色法评估P. fluorescens ZX的生物膜形成能力。【结果】P. fluorescens ZX不同处理液之间对采后锦橙绿霉病的作用效果差异显著,菌悬液抑菌效果最好,经菌悬液处理的果实,发病率和病斑直径分别仅为40.83%和1.78 cm;不论是在柑橘皮固体培养基上对峙培养还是在液体培养基中混合培养,菌悬液和原液的作用效果较好,固体平板上,相对抑制率达到了35%–45%,液体培养基中,P. digitatum孢子12 h后的发芽率不超过27%;P. fluorescens ZX产生的挥发性物质具有抑菌作用,经P. fluorescensZX熏蒸处理的锦橙果实,发病率和病斑直径都显著降低;营养竞争试验结果表明,P. fluorescens ZX能更快速有效地消耗柑橘皮培养基中的营养,并和P. digitatum竞争葡萄糖、果糖、蔗糖、天冬氨酸、苏氨酸、丝氨酸、亮氨酸、精氨酸和脯氨酸等营养物质;同时,P. fluorescens ZX生命力强,培养4 h后即进入对数生长期,约24 h后形成成熟的生物膜。【结论】P. fluorescens ZX可能通过抑制P. digitatum孢子发芽、营养与空间竞争、形成生物膜、产生抑菌物质等方式抑制***的生长繁殖,有效防治采后锦橙绿霉病。

关键词：荧光假单胞菌绿霉病生物防治锦橙采后病害拮抗机制

荧光假单胞菌对采后锦橙青霉病的防治和贮藏品质的影响

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食品与发酵工业 2018年第11期44卷 176-184页

作者：王智荣江孟遥杜木英汪开拓 Zsolt Zalán ferenc hegyi 阚建全西南大学食品科学学院重庆400715 中匈食品科学联合研究中心重庆400715 农业部农产品贮藏保鲜质量安全风险评估实验室(重庆) 重庆400715 重庆三峡学院生命科学与工程学院重庆404100 匈牙利国家农业研究和创新中心食品科学研究所匈牙利布达佩斯H-1022

以"北碚447"锦橙果实为实验材料,研究了荧光假单胞菌(Pseudomonas fluorescens)对采后锦橙青霉病的防治效果和贮藏品质及感官品质的影响。利用P. fluorescens不同浓度菌悬液和各种处理液分别进行离体和活体实验。离体实验结果表明,1×10... 详细信息

以"北碚447"锦橙果实为实验材料,研究了荧光假单胞菌(Pseudomonas fluorescens)对采后锦橙青霉病的防治效果和贮藏品质及感官品质的影响。利用P. fluorescens不同浓度菌悬液和各种处理液分别进行离体和活体实验。离体实验结果表明,1×10~8CFU/mL菌悬液在混合固体培养基上和意大利青霉孢子共同培养时,能完全抑制其生长繁殖,对峙培养时,相对抑菌率高达(52. 59±1. 59)%;在马铃薯葡萄糖液体培养基中意大利青霉孢子12 h后的萌发率和芽管长度分别为(0. 67±0. 47)%和(2. 25±0. 25)μm。活体实验结果表明,P. fluorescens菌悬液抑菌效果最好,且其浓度越高,防治效果越好,浓度为1×10~9CFU/mL时,果实的发病率和病斑直径仅为(49. 17±1. 18)%和(2. 72±0. 09) cm; 4℃下培养16 d,20℃下培养8 d,P. fluorescens在果实伤口处的活菌数分别增长了28倍和34倍;和对照相比,P. fluorescens能显著提高果实几丁质酶和β-1,3-葡聚糖酶的活性,有效增强果实的抗病性;经P. fluorescens菌悬液保鲜的果实,病情指数显著降低,且感官品质和对照差别较小,表明该P. fluorescens试验菌株在锦橙采后病害上具有极高的生物防治潜力。

关键词：荧光假单胞菌生物防治锦橙采后病害贮藏品质感官品质

Restricted Summability of Fourier Transforms and Local Hardy Spaces

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Acta Mathematica Sinica,English Series 2010年第9期26卷 1627-1640页

作者： ferenc WEISZ Department of Numerical Analysis Etvs L. University

A general summability method, the so-called θ-summability is considered for multi-dimensional Fourier transforms. Under some conditions on θ, it is proved that the maximal operator of the θ-means defined in a cone is bounded from the amalgam Hardy space W（hp, e∞） to W（Lp,e∞）. This implies the almost everywhere convergence of the θ-means in a cone for all f ∈ W（L1, e∞） velong to L1.

关键词： Wiener amalgam spaces local Hardy spaces 0-summability of Fourier transforms atomic decomposition

Thermal Reversion of Plant Phytochromes

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Molecular Plant 2020年第3期13卷 386-397页

作者： Cornelia Klose ferenc Nagy Eberhard Schafer Institute of Biology II University of Freiburg79104 FreiburgGermany Institute of Plant Biology Biological Research CentreTemesvari krt.626726 SzegedHungary

Phytochromes are red/far-red reversible photoreceptors essential for plant growth and *** signaling is mediated by the physiologically active far-red-absorbing Pfr form that can be inactivated to the red-absorbing Pr ground state by light-dependent photoconversion or by light-independent thermal reversion,also termed dark *** the term“dark reversion”is justified by historical reasons and frequently used in the literature,“thermal reversion”more appropriately describes the process of light-independent but temperature-regulated Pfr relaxation that not only occurs in darkness but also in light and is used throughout the *** reversion is a critical parameter for the light sensitivity of phytochrome-mediated responses and has been studied for decades,often resulting in contradictory *** reversion is an intrinsic property of the phytochrome molecules but can be modulated by intra-and intermolecular interactions,as well as biochemical modifications,such as *** this review,we outline the research history of phytochrome thermal reversion,highlighting important predictions that have been made before knowing the molecular *** further summarize and discuss recent findings about the molecular mechanisms regulating phytochrome thermal reversion and its functional roles in light and temperature sensing in plants.

关键词： phytochrome phyA phyB thermal reversion dark reversion in vivo spectroscopy

Injury-associated reacquiring of intestinal stem cell function

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World Journal of Gastroenterology 2015年第7期21卷 2005-2010页

作者： ferenc Sipos Gy?rgyi M?zes 2~(nd) Department of Internal Medicine Semmelweis University

Epithelial layer of the intestine relies upon stem cells for maintaining homeostasis and *** types of stem cells are currently defined in intestinal crypts:the cycling crypt base columnar cells and quiescent *** several candidate markers and regulators of rapidly cycling and quiescent stem cells have been identified so far,the exact nature of quiescent cells is still questionable since investigations mainly focused on candidate markers rather than the label-retaining population *** results,however,have streng-thened the argument for functional *** a lineage tracing strategy label-retaining cells(LRCs)of the intestinal epithelium were marked,then followed by a pulse-chase analysis it was found that during homeostasis,LRCs were Lgr5-positive and were destined to become Paneth and neuroendocrine ***,it was demonstrated that LRCs are capable of clonogenic growth by recall to the self-renewing pool of stem cells in case of epithelial *** new findings highlight on the hierarchical and spatial organization of intestinal epithelial homeostasis and the important plasticity of progenitors during tissue regeneration,moreover,provide a motivation for studying their role in disorders like colorectal cancer.

关键词： Epithelium Injury Label-retaining cells Stem cell

Issues and opportunities of stem cell therapy in autoimmune diseases

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World Journal of Stem Cells 2019年第4期11卷 212-221页

作者： Gy?rgyi M?zes ferenc Sipos Immunology Team 2nd Department of Internal MedicineSemmelweis University

The purpose of regenerative medicine is to restore or enhance the normal function of human cells, tissues, and organs. From a clinical point of view, the use of stem cells is more advantageous than differentiated cells because they can be collected more easily and in larger quantities, their proliferation capacity is more pronounced, they are more resistant in cell culture, their aging is delayed, they are able to form a number of cell lines, and they are able to promote vascularization of tissue carriers. The therapeutic use of stem cells for disease modification, immunomodulation, or regenerative purposes are undoubtedly encouraging, but most studies are still in their early stages, and the clinical results reported are not clear with regard to therapeutic efficacy and potential side effects. Uniform regulation of the clinical application of stem cells is also indispensable for this highly customizable, minimally invasive, individualized therapeutic method to become a successful and safe treatment alternative in many different autoimmune and autoinflammatory disorders.

关键词： Stem cell therapy Autoimmune Autoinflammatory Immunomodulation Disease modification

Disagreements in the therapeutic use of mesenchymal stem cellderived secretome

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World Journal of Stem Cells 2022年第6期14卷 365-371页

作者： ferenc Sipos Györgyi Műzes Department of Internal Medicine and Hematology Semmelweis UniversityBudapest 1088Hungary

In a recent article,the authors provide a detailed summary of the characteristics and biological functions of mesenchymal stem cells(MSCs),as well as a discussion on the potential mechanisms of action of MSC-based *** describe the morphology,biogenesis,and current isolation techniques of exosomes,one of the most important fractions of the MSC-derived *** also summarize the characteristics of MSC-derived exosomes and highlight their functions and therapeutic potential for tissue/organ regeneration and for kidney,liver,cardiovascular,neurological,and musculoskeletal diseases,as well as cutaneous wound *** the fact that MSCs are regarded as an important pillar of regenerative medicine,their regenerative potential has been demonstrated to be limited in a number of pathological *** negative effects of MSC-based cell therapy have heightened interest in the therapeutic use of MSC-derived *** the other hand,MSC-derived exosomes and microvesicles possess the potential to have a significant impact on disease development,including *** can interact with tumor cells and promote mutual exchange and induction of cellular markers by exchanging ***,enzymes secreted into and activated within exosomes can result in tumor cells acquiring new *** a result,therapeutic applications of MSC-derived secretomes must be approached with extreme caution.

关键词： Mesenchymal stem cells Secretome Exosomes Regeneration Therapy Cancer