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Identification and Analysis of SSRs Derived from Protein-coding Genes in Grape

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Agricultural Science & Technology 2017年第9期18卷 1579-1584页

作者： Pengfei WANG Ling SU Xilong JIANG Yingchun CHEN fengshan ren Yongmei WANG Shandong Engineering Research Center for Grape Cultivation and Deep-processing Shandong Academy of Grape

SSR(Simple Sequence Repeats), also known as microsatellites or STRs(short tandem repeats), are a type of PCRbased markers. So far, the version of grape genome has been updated constantly, but SSRs derived from protein-coding genes in grape have not yet been identified. In this study, 4 337 SSR-containing genes were found among 29 971 protein-coding genes in grape(Vitis vinifera L.), and 5 384 SSRs were found. There were 96 types of repeat motifs in SSRs derived from protein-coding genes in grape, and the most frequently occurring repeat motif was A/T. Among various repeat motifs in dinucleotide SSRs, the most frequently occurring repeat motif was AG/CT. Moreover, many genes exhibited codon usage bias, which was affected by the mutation pressure. GO annotation, KEGG annotation and domain analysis of these genes were performed.Several genes were found to be closely related to the synthesis and metabolism of secondary metabolites, synthesis of flavones or anthocyanins, development and morphology of plant organs, and tolerance to biotic or abiotic stresses, including transcription factors in MYB, Hsf, NBS and TPC families. This study laid a solid foundation for the development of SSR markers and research of QTLs controlling complex agronomic traits in grape.

关键词：蛋白编码基因 SSR标记标记鉴定葡萄简单重复序列短串联重复序列非生物胁迫次生代谢产物

Genome-wide Identification and Analysis of DNA Methyltransferases in Grape

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Agricultural Science & Technology 2017年第10期18卷 1781-1787,1794页

作者： fengshan ren Liying YANG Ling SU Lei GONG Pengfei WANG Yongmei WANG Shandong Engineering Research Center for Grape Cultivation and Deep-processing Shandong Academy of Grape Ji'nan 250100 China

DNA methylation is an important epigenetic regulation mechanism, which is catalyzed by DNA methyltransferases. In this study, eight DNA methyltransferase genes were identified in grape genome to analyze the selective pressure, gene expression and codon usage bias. The results showed grape DNA methyltransferase MET subfamily underwent relatively strong purifying selection during evolution, while chromomethylase CMT subfamily underwent positive selection during evolution. Under different abiotic(heat, drought or cold) stresses, the expression level of many grape DNA methyltransferase genes changed significantly. The expression level of these genes might be related with cis-regulatory elements of their promoters. The results of codon usage bias analysis showed that synonymous codon bias existed in grape DNA methyltransferase gene family, which might be affected by mutation pressure. These results laid a solid foundation for in-depth study of DNA methyltransferases in grape.

关键词： DNA甲基转移酶基因组葡萄鉴定密码子使用偏性 DNA甲基化顺式调控元件脱氧核糖核酸

Advances in the Role of DNA Methylation and miRNAs in Plant Defense

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Agricultural Biotechnology 2017年第3期6卷 20-22页

作者： Pengfei WANG Xilong JIANG Xinying WU Ling SU fengshan ren Yongmei WANG Shandong Academy of Grape

DNA methylation and miRNAs are two important epigenetic molecular mechmxisms in plants. RNA-directed DNA methylation （RdDM） pathway is con- sidered as the most important mechanism of DNA methylation. A microRNA （miRNA） is processed from a 70-90-nucleotide-long single-stranded RNA precursor that forms a hairpin structure by Dicer enzyme, which plays an important role in plant development, regulation of plant gene expression and silencing of trans- posons. In recent years, a large number of studies have confirmed that DNA methylation and miRNAs play an important role in disease resistance mechanisms of plants. In this paper, recent progress on the role of DNA methylation and miRNAs in plant defense was summarized, which provided reference for revealing the re- lationship between disease resistance factors and epigenctic mechanisms in plants.

关键词： DNA methylation miRNAs Plant defense

Identification of Differentially Expressed Genes in Grape Skin at Veraison and Maturity and Construction of Co-expression Network

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Agricultural Science & Technology 2017年第11期18卷 1993-2000页

作者： Pengfei WANG Xilong JIANG Xinying WU Ling SU Lei GONG Hongmei SHI fengshan ren Yongmei WANG Shandong Engineering Research Center for Grape Cultivation and Deep-processing Shandong Academy of GrapeJi'nan 250100China Key Laboratory of Orban Agriculture in East China Ministry of Agriculture Ji'nan 250100 ChinaChina

The ripening process of grape is an important stage during grape growth and development. During this process, color of grape skin is the most obvious change. The molecular mechanism for the ripening of grape(a non-climacteric fruit, which ripens without ethylene and respiration bursts) is still unclear. Although numerous studies have been done on the changes in the contents of metabolites during grape ripening, the differentially expressed genes at veraison and maturity stages have not been systematically analyzed. In this study, 1 524 genes that are significantly differentially expressed in grape(Pinot Noir) skin at veraison and maturity stages were identified, and a co-expression network of these genes was built. Some of the eight co-expression modules we identified may be closely related to the synthesis or metabolism of anthocyanins, sugar acids, and other flavor substances. The transcription factor families WRKY, b ZIP, HSF and WOX may play an important role in the regulation of anthocyanin synthesis or metabolism. The results provide a foundation for further study of the molecular mechanism of grape ripening.

关键词：差异表达基因成熟过程葡萄皮共表达鉴定网络转熟施工

葡萄VvMET1-1基因在葡萄和拟南芥中的功能研究

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中外葡萄与葡萄酒 2024年第3期 1-12页

作者：王风霞丁亭亭刘蕙萍李科杨国伟李傲张庆田 Chebukin Pavel 任凤山王鹏飞山东省葡萄研究院山东济南250100 俄罗斯瓦维洛夫植物研究所海参崴690014 山东省农业科学院山东济南250100 山东省酿酒葡萄与葡萄酒技术创新中心山东烟台265600

DNA甲基化修饰对于植物的生长发育十分重要,而MET1是植物中重要的甲基转移酶,能维持植物的CG位点甲基化修饰。为了研究葡萄MET1基因的功能及对植物发育的影响,将葡萄VvMET1-1基因转入葡萄胚性愈伤组织和拟南芥中进行研究。本研究选择35S... 详细信息

DNA甲基化修饰对于植物的生长发育十分重要,而MET1是植物中重要的甲基转移酶,能维持植物的CG位点甲基化修饰。为了研究葡萄MET1基因的功能及对植物发育的影响,将葡萄VvMET1-1基因转入葡萄胚性愈伤组织和拟南芥中进行研究。本研究选择35S::VvMET1-1-GFP表达载体转入葡萄胚性愈伤组织后检测到明显的GFP信号,且该胚性愈伤组织能被成功诱导为体细胞胚,表明VvMET1-1首次被成功转入葡萄胚性愈伤组织中并且不影响胚性愈伤组织的发育和分化。VvMET1-1被转入拟南芥中发现,该基因能影响拟南芥叶片的长度和宽度,但是不影响植株形态和种子发育;转录组数据分析表明,许多逆境胁迫、生长素、种子萌发、果实成熟、脱落酸和茉莉酸合成相关的基因差异表达;甲基化组结果显示,拟南芥过表达VvMET1-1后CG和CHH位点甲基化水平分别发生了明显的上调和下调,且DNA甲基化差异区(DMR)的类型主要是CG和CHH型,因此推测VvMET1-1可能通过调控DNA甲基化水平的变化影响下游基因的表达,进而调控拟南芥的生长发育。

关键词：葡萄胚性愈伤组织 VvMET1-1 转录组测序

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Research on the Necessity of Building National-level Grape and Cherry Germplasm Resources Bank in Shandong Province

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Agricultural Biotechnology 2018年第2期7卷 36-39页

作者： Pengfei WANG fengshan ren Yongmei WANG Xinying WU Xilong JIANG Yingchun CHEN Shan WANG Qian MU Liying YANG Wanjun CHEN Shandong Engineering Research Center for Grape Cultivation and Deep-processing Shandong Academy of GrapeJinan 250100China Key Laboratory of Urban Agriculture(East China) Ministry of AgricultureJinan 250100China

At present,the grape and cherry industries in Shandong are in the leading position in China and have driven the development of related high-end manufacturing industries such as wine,brandy,preserved fruit processing,fruit juice processing and health products. Therefore,vigorously developing the grape and cherry industries in Shandong Province and carrying out development and innovation are important parts of Shandong Province in responding to the strategy of national new and old kinetic energy conversion. However,currently the germplasm resources preserved in the fruit tree resources banks in China are only 45% of those in the US and 27. 2% of those in the EU. Moreover,the development of fruit trees resources banks in Shandong is relatively backward in China,and there is still no banks related with grape and cherry germplasm resources in Shandong. Therefore,importance can be attached to the agriculture,forestry,and animal husbandry to build germplasm resources banks for grape and cherry industries in Shandong Province. Building the national-level grape and cherry germplasm resources bank in Shandong Province can also promote the utilization of wild and farm germplasm resources in the future; advance the research on the genes related to disease resistance,stress resistance and quality of grapes and cherries; push forward the construction and development of cherry and grape mutants banks. It is conducive to the research on the agronomic traits of grapes and cherries,and can provide the parents resources for planting innovation and improving the quality of grapes and cherries,as well as promote the development and application of molecular markers of grapes and cherries,including the identification of lines and crossbreeding. Thereby,it cannot only promote the industry development,but also achieve the development of cultivation,breeding and basic research in an all-round way and the development of ＂ production,study and research＂ going side by side.

关键词： New and old kinetic energy conversion Middle-income trap Grape Cherry Germplasm resources bankHome

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橡胶水泥土动剪模量和阻尼比共振柱试验研究

振动与冲击 2024年第12期43卷 266-275页

作者：郭端伟何杰宋德新王忍李凤山湖南工业大学土木工程学院湖南株洲412007 交通运输部南海航海保障中心广州航标处广州510320 湖南省第二工程有限公司长沙410015

橡胶水泥土作为一种经济环保的耐腐蚀轻质填筑材料,在沿海港口工程及灯塔等基础建设中具有广泛的应用前景。基于共振柱试验,对不同橡胶掺量、水泥掺量和围压下橡胶水泥土动力特性展开研究,重点探究各影响因素对其动剪模量和阻尼比的影... 详细信息

橡胶水泥土作为一种经济环保的耐腐蚀轻质填筑材料,在沿海港口工程及灯塔等基础建设中具有广泛的应用前景。基于共振柱试验,对不同橡胶掺量、水泥掺量和围压下橡胶水泥土动力特性展开研究,重点探究各影响因素对其动剪模量和阻尼比的影响规律。结果表明:固结压力下橡胶水泥土的累计轴向应变随着橡胶掺量和围压的增大而增大,随水泥掺量增大而减小;橡胶水泥土动剪模量曲线衰减程度随橡胶掺量的增加和水泥掺量的减小而减弱,非线性特征减弱,而受围压影响则较小;最大动剪模量随着橡胶掺量的增大而减小,随水泥掺量和围压的增大而增大。当橡胶掺量较低或水泥掺量较高时,其掺量改变对最大动剪模量影响最大;橡胶的掺入减缓了动剪模量衰减幅度,并且在较低围压下促使其更早地发生衰减。而水泥掺量的减小和围压的增大则会推迟衰减,衰减幅度也相对更大;阻尼比随动剪应变的增加而单调递增,增大橡胶掺量和减小围压会使其阻尼比增大,当水泥掺量小于15%时,阻尼比随水泥掺量增加而增大,而当水泥掺量大于15%时,阻尼比反而随之降低。

关键词：橡胶水泥土动剪模量阻尼比配合比共振柱试验

5种杀菌剂防治番茄茎腐病的室内筛选和田间使用效果

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农学学报 2016年第5期6卷 18-22页

作者：任凤山高亮张博山东省农业科学院植物保护研究所/山东省植物病毒学重点实验室济南250100 山东农业大学植物保护学院山东泰安271018

笔者旨在通过筛选防治番茄茎腐病的有效药剂,为田间防治提供指导。室内测定了5种杀菌剂对终极腐霉菌丝生长的抑制作用,恶霉灵、唑醚·代森联、硫酸铜钙、甲基硫菌灵和恶酮·霜脲氰为有效杀菌剂。研究结果表明:恶霉灵的EC50为0.364 mg/L... 详细信息

笔者旨在通过筛选防治番茄茎腐病的有效药剂,为田间防治提供指导。室内测定了5种杀菌剂对终极腐霉菌丝生长的抑制作用,恶霉灵、唑醚·代森联、硫酸铜钙、甲基硫菌灵和恶酮·霜脲氰为有效杀菌剂。研究结果表明:恶霉灵的EC50为0.364 mg/L,抑菌效果最好;唑醚·代森联效果次之,EC50为9.247 mg/L,其他药剂防治效果相对较差。在田间药剂筛选试验中,70%恶霉灵可湿性粉剂与70%甲基硫菌灵可湿性粉剂组合防治效果为95.57%,均优于其它供试药剂组合,可应用于番茄茎腐病的田间防治。

关键词：番茄终极腐霉茎腐病杀菌剂化学防治

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系统性红斑狼疮免疫学检查的临床意义

哈尔滨医科大学学报 1987年第5期 36-39页

作者：张凤山任潞雪哈尔滨医科大学二院中医科

本文以作者临床实践为基础,对目前临床应用较有实用价值的免疫检查、LE细胞、ANA（抗核抗体）、anti-ds DNAab、anti Smab、LBT（皮肤免疫荧光带试验）等的临床意义进行评价,并结合文献复习,对免疫复合物、补体、细胞免疫异常等检测的... 详细信息

本文以作者临床实践为基础,对目前临床应用较有实用价值的免疫检查、LE细胞、ANA（抗核抗体）、anti-ds DNAab、anti Smab、LBT（皮肤免疫荧光带试验）等的临床意义进行评价,并结合文献复习,对免疫复合物、补体、细胞免疫异常等检测的临床意义作了初步讨论。强调了ANA检查的高敏感性及抗ds DNA抗体、抗Sm抗体、非暴光“正常”皮肤LBT检查对本病早期诊断的高特异性。

关键词：系统性红斑狼疮免疫学检查