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当归挥发油平滑肌效应研究进展

中兽医医药杂志 2018年第4期37卷 90-92页

作者：孙敏陈振鹤刘峰林任远甘肃中医药大学甘肃兰州730000 甘肃省中药药理与毒理学重点实验室甘肃兰州730000

随着对当归挥发油研究的深入,其药理活性和潜在的应用前景也逐渐被认识和期望。笔者等对当归挥发油的平滑肌效应及其主要成分之一的苯酞类对平滑肌的作用进行综述,为当归挥发油平滑肌效应的药效物质研究提供参考。

关键词：当归平滑肌效应主要药效物质

基于网络药理学-分子对接及实验研究探讨当归效应组分治疗原发性痛经的作用机制

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中药药理与临床 2023年第1期39卷 49-55页

作者：王瑞琼孙敏吴国泰王志旺刘峰林谢田朋任远甘肃中医药大学兰州730000 甘肃省中药药理与毒理学重点实验室兰州730000

目的:采用网络药理学-分子对接的方法,探讨当归效应组分治疗原发性痛经(PD)的物质基础及作用机制,并进行动物实验验证。方法:利用气相色谱-质谱联用仪(GC-MS)检测当归效应组分的化学成分;通过TCMSP、UniProt Database、Pubchem、Swiss T... 详细信息

目的:采用网络药理学-分子对接的方法,探讨当归效应组分治疗原发性痛经(PD)的物质基础及作用机制,并进行动物实验验证。方法:利用气相色谱-质谱联用仪(GC-MS)检测当归效应组分的化学成分;通过TCMSP、UniProt Database、Pubchem、Swiss Target Pridiction数据库检索当归效应组分化学成分的作用靶点;采用TTD、Drugbank、pharmGKB、Uniprot、DisGeNET数据库检索PD相关的靶点,并建立数据集;筛选成分靶点和疾病靶点映射的共同靶点;利用David和KOBAS数据库对共同靶点进行通路的富集与分析;采用Cytoscape软件构建“活性成分-靶点-通路”的可视化网络关系图,分析当归效应组分治疗PD的物质基础及作用机制;以Discovery Studio软件对网络模型分析结果进行分子对接验证;根据预测结果进一步进行实验验证:将72只雌性KM小鼠随机分为正常对照组、模型对照组、布洛芬0.07 g/kg组、丁苯酞0.05、0.10、0.20 g/kg组,每组12只。采用戊酸雌二醇联合缩宫素建立小鼠原发性痛经模型。观察丁苯酞对模型小鼠扭体行为的影响,检测血清中E_(2)、P、NO、PGF_(2α)含量及子宫组织中ER、PR蛋白表达。结果:分析并鉴定了当归效应组分中44种化学成分;检索得到对应靶点809个,映射得到与PD相关靶点53个,发现其中PTGS2、PTGS1、PPARG、UGT2B4、CA2、ALOX5、UGT2B7等13个靶点可能为当归效应组分治疗PD的重要潜在靶点;通路注释分析得到47条作用通路,其中花生四烯酸代谢、卵巢类固醇生成、类固醇激素生物合成、催产素信号通路、NF-κB信号通路等15条与PD相关;网络图结果显示,3-丁烯基苯酞、藁本内酯、β-金合欢烯、β-花柏烯、β-红没药烯、β-柏木烯等10个核心成分可能是当归治疗PD的重要药效物质;分子对接结果显示,3-丁烯基苯酞、藁本内酯与核心靶点度值前3位的靶蛋白(PTGS2、PTGS1、PPARG)有较好的结合性。动物实验结果显示,丁苯酞能延长原发性痛经模型小鼠扭体发生的潜伏期,降低扭体反应的发生次数,升高血清中NO、P含量而降低E2、PGF_(2α)的含量,下调子宫组织中ER蛋白表达而上调PR蛋白表达,ER/PR值下降(P<0.05或P<0.01)。结论:当归效应组分通过多成分、多靶点、多通路治疗PD,作用机制可能与激素调节、中枢镇痛、解痉、抗炎及能量代谢有关。

关键词：当归效应组分原发性痛经网络药理学分子对接作用机制

ASYMMERTIC LEAVES1/2 ENHANCER7,a novel gene required for BREVIPEDICELLUS repression and leaf adaxial-abaxial polarity establishment in Arabidopsis

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ASYMMERTIC LEAVES1/2 ENHANCER7,a novel gene required for BRE...

上海市植物生理学会成立三十周年庆祝活动暨第三届上海市植物生理学青年学术研讨会

作者： Zhenhuan yuan, Hua Wang, Guang Li, Xiaozhen Yao, Dexian Luo, Minhuan Zeng, Hai Huang, Xiaofeng Cui* National Laboratory of Plant Molecular Genetics, Shanghai Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 300 fenglin Road, Shanghai 200032, China

The Arabidopsis ASYMMETRIC LEAVES1 and 2 (AS1 and AS2) genes, both encoding transcription factors, play important roles in leaf morphogenesis. AS1 and AS2 are known to

关键词： Arabidopsis Leaf polarity as1/2 enhancer7 BREVIPEDICELLUS repression

A biomaterial-based therapy for lower limb ischemia using Sr/Si bioactive hydrogel that inhibits skeletal muscle necrosis and enhances angiogenesis

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Bioactive Materials 2023年第8期26卷 264-278页

作者： Ye yuan Zhaowenbin Zhang Fandi Mo Chen Yang Yiren Jiao Enci Wang Yuchong Zhang Peng Lin Chengkai Hu Weiguo Fu Jiang Chang Lixin Wang Department of Vascular Surgery Zhongshan HospitalFudan University180 Fenglin RoadShanghai200032China Department of Vascular Surgery Zhongshan Xiamen HospitalFudan University668 JinhuRoadXiamen361015China Joint Centre of Translational Medicine The First Affiliated Hospital of Wenzhou Medical UniversityWenzhou325000China Zhejiang Engineering Research Center for Tissue Repair Materials Wenzhou InstituteUniversity of Chinese Academy of SciencesWenzhou325000China Shanghai Institute of Ceramics Chinese Academy of Sciences1295 Dingxi RoadShanghai200050China

Muscle necrosis and angiogenesis are two major challenges in the treatment of lower-limb ischemic *** this study,a triple-functional Sr/Si-containing bioceramic/alginate composite hydrogel with simultaneous bioactivity in enhancing angiogenesis,regulating inflammation,and inhibiting muscle necrosis was designed to treat lower-limb ischemic *** particular,sodium alginate,calcium silicate and strontium carbonate were used to prepare injectable hydrogels,which was gelled within 10 *** importantly,this composite hydrogel sustainedly releases bioactive Sr^(2+)and SiO_(3)^(2-) ions within 28 *** biological activity of the bioactive ions released from the hydrogels was verified on HUVECs,SMCs,C2C12 and Raw 264.7 cells in vitro,and the therapeutic effect of the hydrogel was confirmed using C57BL/6 mouse model of femoral artery ligation in *** results showed that the composite hydrogel stimulated angiogenesis,developed new collateral capillaries,and re-established the blood *** addition,the bioactive hydrogel directly promoted the expression of muscle-regulating factors(MyoG and MyoD)to protect skeletal muscle from necrosis,inhibited M1 polarization,and promoted M2 polarization of macrophages to reduce inflammation,thereby protecting skeletal muscle cells and indirectly promoting *** results indicate that these bioceramic/alginate composite bioactive hydrogels are effective biomaterials for treating hindlimb ischemia and suggest that biomaterial-based approaches may have remarkable potential in treating ischemic diseases.

关键词： Hindlimb ischemic diseases Strontium carbonate and calcium silicate bioceramic Bioactive hydrogel Muscle necrosis Angiogenesis

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浅谈“多表合一”在智慧城市中的推广应用

电测与仪表 2018年第B08期55卷 68-70,89页

作者：孙逢麟孙艳玲刘源宋腾飞任树合张涛国网山东省电力公司电力科学研究院济南250002 国网山东省电力公司滨州供电公司山东滨州256600 国网山东省电力公司烟台供电公司山东烟台264000

推进"多表合一"信息采集系统建设,是推进互联网+智慧城市建设,解决为民服务"最后一公里"问题的创新实践。国家电网公司结合水气热各行业表计的不同通信方式,通过调研水、气、热表市场和技术现状,研究设计了相应采集技术方案,编制了多项... 详细信息

推进"多表合一"信息采集系统建设,是推进互联网+智慧城市建设,解决为民服务"最后一公里"问题的创新实践。国家电网公司结合水气热各行业表计的不同通信方式,通过调研水、气、热表市场和技术现状,研究设计了相应采集技术方案,编制了多项技术标准,通过采用新技术、研发新设备攻克了多项难题,为各地智慧城市建设提供了技术支撑,让智慧城市更近一步。

关键词：多表合一智慧城市信息采集

高效液相色谱法同时测定牡蒿叶中4个绿原酸类成分含量

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浙江中医药大学学报 2021年第6期45卷 675-684页

作者：于凤琳袁丹陈宇李美芽窦晓兵蒋福升浙江中医药大学杭州310053

[目的]通过响应面法优化提取工艺,并结合高效液相色谱(high performance liquid chromatography,HPLC)法测定牡蒿叶中4个绿原酸类成分的含量,探究其开发利用价值。[方法]采用Thermo Scientific Syncronis Amino色谱柱(4.6mm×250mm,5μm)色谱条件为流动相乙腈:0.1%甲酸水(40:60,V/V),流速1mL·min^(-1),柱温25℃检测波长325nm;以绿原酸、异绿原酸A、异绿原酸B和异绿原酸C的总含量为响应值乙醇浓度、液料比、超声功率为主要因素采用Box-Behnken响应面法对牡蒿叶中4个绿原酸类成分进行优化提取,并测定其含量。[结果]以乙腈:0.1%甲酸水(40:60,V/V)等度洗脱,流速1mL·min^(-1),柱温25℃检测波长325nm,牡蒿叶中绿原酸、异绿原酸A、异绿原酸B和异绿原酸C分离度良好,在6.25~200.00μg·mL^(-1)浓度范围内线性关系良好加样回收、精密度、稳定性和重复性均符合要求。优化得到的最佳提取工艺为:乙醇浓度60%,液料比40:1(单位mL·g^(-1))超声功率250W频率53kHz室温超声20min。通过HPLC法测得绿原酸、异绿原酸A、异绿原酸B和异绿原酸C含量分别为14.3170mg·g^(-1)、27.9452mg·g^(-1)、1.2304mg·g^(-1)和5.0665mg·g^(-1),合计总量达48.5590mg·g^(-1)。[结论]HPLC法测得牡蒿叶中绿原酸类成分含量较为丰富。采用响应面法优化提取工艺,能够显著提高牡蒿叶中4个绿原酸类成分提取率具有较好潜在开发应用价值。

关键词：牡蒿绿原酸异绿原酸高效液相色谱法响应面法超声提取提取工艺含量测定

Firing properties of identified airway-related preganglionic parasympathetic neurons in the ventrolateral medulla of neonatal rat

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Firing properties of identified airway-related preganglionic...

中国神经科学学会第九届全国学术会议暨第五次会员代表大会

作者： Yong-Hua CHEN~1,Li-Li HOU~(1,2),Xu-Jiao ZHOU~1,Dong-Ying QIU~(1,2),Lei ZHU~2,Ji-Jiang WANG~(1,*) 1 The State Key Laboratory of Medical Neurobiology and Institute of Brain Sciences,Fudan University Shanghai Medical College,138 Ti-Xue-yuan Road,Shanghai 200032,China 2 Fudan University Zhongshan Hospital,180 fenglin Road,Shanghai 200032,China.

The airway function is dominated by the parasympathetic nervous system.A subpopulation of the airwayrelated preganglionic parasympathetic neurons(APPNs) located in the external formation of the nucleus ambiguus (eNA) play critical roles in the physiological and pathological control of tracheobronchial smooth ***, the firing properties of these APPNs are not *** the present study,APPNs in the eNA were retrogradely labeled with a fluorescent tracer applied to the extrathoracic trachea and identified in rhythmically firing brainstem slices,and their electrophysiological properties were examined using patch-clamp *** results showed that inspiratory and expiratory APPNs in the eNA were relatively dissociated *** of the sodium channels in both types of neurons showed L-shaped current-voltage relationship *** both types of neurons depolarization evoked a transient outward potassium current that was sensitive to 4-aminopyridine(4-AP),and a lasting outward potassium current that was partially sensitive to 4-AP in inspiratory APPNs and insensitive to 4-AP in expiratory *** types of neurons have an afterhyperpolarization(AHP) after each action potential,which was mediated by smallconductance Ca-activated K channel(SK) in inspiratory APPNs and by large-conductance Ca-activated K channel(LK) in expiratory *** findings have led to better understanding of the physiological properties of the APPNs controlling the tracheal or tracheobronchial muscles.

关键词： airway electrophysiology patch-clamp

推进系统完全封闭管路内压力爬升速率的量化研究

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化学推进剂与高分子材料 2024年第3期22卷 42-45页

作者：高永宇文雷丁凤林王渊翟阔阔北京控制工程研究所北京100190 北京市高效能及绿色宇航推进工程技术研究中心北京100190

为了量化评估推进系统完全封闭管路中的压力爬升速率,搭建了无水肼管路地面模拟试验系统,将试验结果与基于状态方程的理论方法和基于试验数据的经验方法进行了对比分析。结果表明,基于状态方程的理论方法能够准确表征不同温度下管路压... 详细信息

为了量化评估推进系统完全封闭管路中的压力爬升速率,搭建了无水肼管路地面模拟试验系统,将试验结果与基于状态方程的理论方法和基于试验数据的经验方法进行了对比分析。结果表明,基于状态方程的理论方法能够准确表征不同温度下管路压力爬升速率的变化趋势,但在绝对值上与试验结果相差较大;基于试验数据的经验方法在压力爬升速率的绝对值上与地面试验结果较为接近,但不能反映不同温度下压力爬升速率变化趋势上的差异。通过修正基于状态方程的理论方法中的常数项,提出了一种半经验性的改进算法,更符合试验结果。改进算法的仿真结果表明,在10~40℃,完全封闭管路内无水肼的压力爬升速率为1.701~1.932MPa/℃。

关键词：单组元推进系统无水肼状态方程管路体积膨胀系数

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Thyrotropin-releasing hormone excited preganglionic inspiratory tracheal vagal motoneurones via multiple mechanisms

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Thyrotropin-releasing hormone excited preganglionic inspirat...

中国神经科学学会第九届全国学术会议暨第五次会员代表大会

作者： Li-Li HOU~(1,2),Xu-Jiao ZHOU~1,Yong-Hua CHEN~1,Dong-Ying QIU~(1,3),Lei ZHU~2,Ji-Jiang WANG~(1,*) 1 The State Key Laboratory of Medical Neurobiology and Institute of Brain Sciences,Fudan University Shanghai Medical College,138-Yi-Xue-yuan Road,Shanghai 200032,China 2 Department of Respiratory Medicine,Fudan University Zhongshan Hospital,180 fenglin Road,Shanghai 200032,China.3 Department of Gerontology,Fudan University Zhongshan Hospital,180 fenglin Road,Shanghai 200032,China

The inspiratorily activated preganglionic tracheobronchial vagal motoneurones(I-TVMs) in the nucleus ambiguus,which predominate in the central control of the tracheobronchial smooth muscles,receive dense inputs from terminals containing thyrotropin-releasing hormone(TRH).TRH microinjection into the nucleus ambiguus caused constriction of the tracheal smooth muscles however the neuronal and synaptic mechanisms are *** this study, the I-TVMs were retrogradedly labeled from the extrathoracic trachea of newborn rats,identified in rhythmically firing brainstem slices,and the effects of TRH were examined using *** voltage clamp TRH(100 nmol L) caused a slow excitatory inward current,enhanced the excitatory inputs and evoked a distinct oscillatory pattern of the baseline *** slow excitatory inward current and the oscillatory pattern were unaffected by blockade of chemical *** junction blocker carbenoxolone(100μmol L) prevented the oscillatory pattern without affecting the slow excitatory inward current and the enhancement of the excitatory ***(1μmol L),a blocker of both the voltage-dependent sodium transient and persistent sodium current,riluzole(20μmol L),a blocker of the persistent the sodium current,and cadmium chloride(200μmol L),a non-selective blocker of the voltagedependent calcium channels,each blocked a major proportion of the slow excitatory inward current and prevented the oscillatory *** current clamp TRH caused a slow depolarization and continuous or oscillatory firing. These results demonstrate that TRH excited the I-TVMs presynaptically via enhancement of the excitatory inputs,and postsynaptically via a slow excitatory inward current and a gap junction-mediated oscillatory pattern.

关键词： thyrotropin-releasing hormone oscillation vagal neuron