检索结果-南通市图书馆

CNKSR2 interactome analysis indicates its association with the centrosome/microtubule system

在线全文

学校读者我要写书评

暂无评论

Neural Regeneration Research 2025年第8期20卷 2420-2432页

作者： Lin Yin Yalan Xu jie Mu Yu Leng Lei Ma Yu Zheng Ruizhi Li Yin Wang Peifeng Li Hai Zhu Dong Wang Jing Li Institute for Translational Medicine The Affiliated Hospital of Qingdao UniversityMedical CollegeQingdao UniversityQingdaoShandong ProvinceChina School of Basic Medicine Qingdao UniversityQingdaoShandong ProvinceChina College of Life Sciences and School of PharmacyMedical CollegeQingdao UniversityQingdaoShandong ProvinceChina Department of Urology Qingdao Municipal Hospital Affiliated to Qingdao UniversityQingdaoShandong ProvinceChina

The protein connector enhancer of kinase suppressor of Ras 2(CNKSR2),present in both the postsynaptic density and cytoplasm of neurons,is a scaffolding protein with several protein-binding *** of the CNKSR2 gene have been implicated in neurodevelopmental disorders,particularly intellectual disability,although the precise mechanism involved has not yet been fully *** has demonstrated that CNKSR2 plays a role in facilitating the localization of postsynaptic density protein complexes to the membrane,thereby influencing synaptic signaling and the morphogenesis of dendritic ***,the function of CNKSR2 in the cytoplasm remains to be *** this study,we used immunoprecipitation and high-resolution liquid chromatography-mass spectrometry to identify the interactors of *** a combination of bioinformatic analysis and cytological experiments,we found that the CNKSR2 interactors were significantly enriched in the proteome of the *** also showed that CNKSR2 interacted with the microtubule protein DYNC1H1 and with the centrosome marker *** colocalization analysis confirmed the centrosomal localization of *** we downregulated CNKSR2 expression in mouse neuroblastoma cells(Neuro 2A),we observed significant changes in the expression of numerous centrosomal *** manipulation also affected centrosome-related functions,including cell size and shape,cell proliferation,and ***,we found that CNKSR2 interactors were highly enriched in de novo variants associated with intellectual disability and autism spectrum *** findings establish a connection between CNKSR2 and the centrosome,and offer new insights into the underlying mechanisms of neurodevelopmental disorders.

关键词： autism spectrum disorder centrosome CNKSR2 intellectual disability interactome mass spectrometry microtubule neurodevelopmental disease protein complexes protein-protein interactions

Nanocarrier-mediated siRNA delivery:a new approach for the treatment of traumatic brain injury-related Alzheimer's disease

在线全文

学校读者我要写书评

暂无评论

Neural Regeneration Research 2025年第9期20卷 2538-2555页

作者： jie Jin Huajing Zhang Qianying Lu Linqiang Tian Sanqiao Yao Feng Lai Yangfan Liang Chuanchuan Liu Yujia Lu Sijia Tian Yanmei Zhao Wenjie Ren Institute of Disaster and Emergency Medicine Tianjin UniversityTianjinChina Key Laboratory for Disaster Medicine Technology TianjinChina Henan Medical Key Laboratory for Research of Trauma and Orthopedics The Third Affiliated Hospital of Xinxiang Medical UniversityXinxiangHenan ProvinceChina Clinical Medical Center of Tissue Engineering and Regeneration Xinxiang Medical UniversityXinxiangHenan ProvinceChina Institutes of Health Central Plain Xinxiang Medical UniversityXinxiangHenan ProvinceChina School of Public Health Xinxiang Medical UniversityXinxiangHenan ProvinceChina

Traumatic brain injury and Alzheimer's disease share pathological similarities,including neuronal loss,amyloid-βdeposition,tau hyperphosphorylation,blood-brain barrier dysfunction,neuroinflammation,and cognitive ***,traumatic brain injury can exacerbate Alzheimer's disease-like pathologies,potentially leading to the development of Alzheimer's *** offer a potential solution by facilitating the delive ry of small interfering RNAs across the blood-brain barrier for the targeted silencing of key pathological genes implicated in traumatic brain injury and Alzheimer's disease.U nlike traditional approaches to neuro regeneration,this is a molecula r-targeted strategy,thus avoiding non-specific drug *** review focuses on the use of nanocarrier systems for the efficient and precise delive ry of siRNAs,discussing the advantages,challenges,and future *** principle,siRNAs have the potential to target all genes and non-targetable protein s,holding significant promise for treating various *** the various therapeutic approaches currently available for neurological diseases,siRNA gene silencing can precisely"turn off"the expression of any gene at the genetic level,thus radically inhibiting disease progression;however,a significant challenge lies in delivering siRNAs across the blood-brain *** have received increasing attention as an innovative drug delive ry tool fo r the treatment of brain *** are considered a potential therapeutic strategy with the advantages of being able to cross the blood-brain barrier,targeted drug delivery,enhanced drug stability,and multifunctional *** use of nanoparticles to deliver specific modified siRNAs to the injured brain is gradually being recognized as a feasible and effective *** this strategy is still in the preclinical exploration stage,it is expected to achieve clinical translation in the future,creating a new field of molecular tar

关键词： blood-brain barrier cognitive dysfunction exosomes gene silencing immunotherapy inorganic nanomaterials liposome nanoparticles neuroinflammation polymer nanoparticles stem cells

在线全文

学校读者我要写书评

暂无评论

新加坡的语言政策与国家安全

外语学刊 2025年第1期 113-118页

作者：陈志杰南京信息工程大学新加坡研究中心南京210044

新加坡的语言政策与国家身份建构息息相关,因而一直受到学界的关注。然而,国家安全是新加坡国家身份建构的前提。因此,本文从国家安全的视角出发,分析了语言政策在新加坡的国际政治安全、国内社会安全、经济安全和文化安全等方面所起的... 详细信息

新加坡的语言政策与国家身份建构息息相关,因而一直受到学界的关注。然而,国家安全是新加坡国家身份建构的前提。因此,本文从国家安全的视角出发,分析了语言政策在新加坡的国际政治安全、国内社会安全、经济安全和文化安全等方面所起的作用,指出新加坡的双语制制造了新加坡语言身份与文化身份的割裂,在一定程度上阻碍了新加坡文化安全的实现,可能最终威胁到新加坡的国家安全。

关键词：新加坡语言政策国家安全文化身份语言身份

Antithetical impacts of deleterious LRP1B mutations in non-squamous and squamous NSCLCs on predicting benefits from immune checkpoint inhibitor alone or with chemotherapy over chemotherapy alone: retrospective analyses of the POPLAR/OAK and CHOICE-01 trial

在线全文

学校读者我要写书评

暂无评论

Science China Life Sciences 2025年第1期 249-262页

作者： Jinliang Wang Wenyong Zhou Yu Xu Jianchun Duan Qiaoxia Zhou Guoqiang Wang Leo Li Chunwei Xu Wenxian Wang Shangli Cai Zhijie Wang jie Wang Department of Oncology The Fifth Medical Center of Chinese PLA General Hospital Department of Thoracic Surgery Shanghai Chest HospitalSchool of MedicineShanghai Jiao Tong University Burning Rock Biotech CAMS Key Laboratory of Translational Research on Lung Cancer State Key Laboratory of Molecular OncologyDepartment of Medical OncologyNational Cancer Center/National Clinical Research Center for ancer/Cancer HospitalChinese Academy of Medical Sciences and Peking Union Medical College Institute of Basic Medicine and Cancer (IBMC) Chinese Academy of Sciences Department of Clinical Trial The Cancer Hospital of the University of Chinese Academy of Sciences (Zhejiang Cancer Hospital)

In non-small cell lung cancers,the non-squamous and squamous subtypes（nsqNSCLC and sqNSCLC） exhibit disparities in pathophysiology,tumor immunology,and potential genomic correlates affecting responses to immune checkpoint inhibitor（ICI）-based *** our inhouse training cohort（n=85）,the presence of the LRP1B deleterious mutation（LRP1B-del） was associated with longer and shorter progression-free survival（PFS） on ICIs alone in nsqNSCLCs and sqNSCLCs,respectively(Pinteraction=0.008).These results were validated using a larger public ICI cohort(n=208,Pinteraction<0.001).Multiplex immunofluorescence staining revealed an association between LRP1B-del and increased and decreased numbers of tumor-infiltrating CD8+T cells in nsqNSCLCs（P=0.040） and sqNSCLCs（P=0.014）,*** the POPLAR/OAK cohort,nsqNSCLCs with LRP1B-del demonstrated improved PFS benefits from atezolizumab over docetaxel(hazard ratio（HR）=0.70,P=0.046),whereas this benefit was negligible in those without LRP1B-del（HR=1.05,P=0.64）.Conversely,sqNSCLCs without LRP1B-del benefited more from atezolizumab（HR=0.60,P=0.002） than those with LRP1B-del（HR=1.30,P=0.31）.Consistent results were observed in the in-house CHOICE-01 cohort,in which nsqNSCLCs with LRP1B-del and sqNSCLCs without LRP1B-del benefited more from toripalimab plus chemotherapy than from chemotherapy alone(Pinteraction=0.008).This multi-cohort study delineates the antithetical impacts of LRP1B-del in nsqNSCLCs and sqNSCLCs on predicting the benefits from ICI alone or with chemotherapy over chemotherapy *** findings highlight the distinct clinical utility of LRP1B-del in guiding treatment choices for nsqNSCLCs and sqNSCLCs,emphasizing the necessity for a detailed analysis based on pathological subtypes when investigating biomarkers for cancer therapeutics.

关键词：

同方期刊数据库

在线全文

学校读者我要写书评

暂无评论

多尺度差分特征增强网络的遥感影像变化检测

计算机科学与探索 2025年第1期19卷 211-222页

作者：王杰蒋伏松蒋鹏上海海洋大学工程学院上海交通大学医学院附属第六人民医院内分泌代谢科上海交通大学医学院附属第六人民医院计算机中心

遥感影像变化检测旨在识别不同时期遥感影像中的目标差异，近年来基于卷积神经网络的方法在遥感影像变化检测任务中取得了很大进展。然而，受光照变化和季节更替的影响，不同时期遥感影像中的伪变化问题仍然难以解决。同时，大多方法中... 详细信息

遥感影像变化检测旨在识别不同时期遥感影像中的目标差异，近年来基于卷积神经网络的方法在遥感影像变化检测任务中取得了很大进展。然而，受光照变化和季节更替的影响，不同时期遥感影像中的伪变化问题仍然难以解决。同时，大多方法中未充分利用多尺度特征，导致模型的性能和准确率受到一定程度的限制。针对上述问题，提出一种多尺度差分特征增强的变化检测方法。利用由孪生网络编码器和差分网络编码器组成的并行编码框架分别提取不同层级的特征，将同级的双时特征和差分特征通过拼接的方式建立两者间的互补关系。引入差分特征增强模块获取更具判别性的特征图并将其作为差分网络编码器的补充输入，丰富变化信息的同时增加模型对变化区域的关注度，使其准确地区分地物的真实变化与伪变化。为了增强特征的多样性和表达能力，使用特征错位融合模块实现语义特征的交叉融合，让每个特征中的语义信息得到充分而不同的交互。该方法在CDD数据集和LEVIRCD数据集上的F1分数分别达到了95.45%和92.04%，交并比分别达到了92.26%和82.93%，与其余八种主流方法相比均为最优，实验结果证明了该方法的有效性。

关键词：遥感影像变化检测差分增强并行编码多尺度特征融合

同方期刊数据库

乒乓球运动者正手弧圈球不同触地模式的下肢生物力学变化

在线全文

学校读者我要写书评

暂无评论

中国组织工程研究 2025年第14期29卷 2995-3001页

作者：晋鹏飞任杰上海体育大学中国乒乓球学院上海市200438

背景:探讨基于步法的足部触地模式对乒乓球运动中下肢关节运动特征的影响,可为指导下肢训练提供科学依据。目的:对比乒乓球运动中不同触地模式下的运动学和动力学差异。方法:使用动作捕捉和三维测力台同步采集17名专业乒乓球运动员在正... 详细信息

背景:探讨基于步法的足部触地模式对乒乓球运动中下肢关节运动特征的影响,可为指导下肢训练提供科学依据。目的:对比乒乓球运动中不同触地模式下的运动学和动力学差异。方法:使用动作捕捉和三维测力台同步采集17名专业乒乓球运动员在正常触地和全脚掌触地模式下优势侧下肢的运动学和动力学数据。配对样本t检验用于分析关节角度、地面反作用力、关节反作用力和关节力矩等离散指标的统计学差异;统计参数映射用于分析足底压力中心移动轨迹和膝关节力矩等时间序列数据的统计学差异。结果与结论:①正常触地模式下的膝关节内翻、侧向地面反作用力、膝关节垂直反作用力和伸膝力矩峰值显著高于全脚掌触地模式;②在足底压力中心移动距离中的89%-91%运动阶段,正常触地模式压力中心显著远于全脚掌触地模式;③在X轴力矩曲线中的23%-38%运动阶段,正常触地模式的伸膝力矩显著大于全脚掌触地模式;④在Y轴力矩曲线的8%-18%运动阶段,全脚掌触地模式显著大于正常触地模式。通过对比不同触地模式的下肢生物力学特征值和统计参数映射分析结果,认为全脚掌触地模式可能是影响乒乓球运动表现的重要因素。乒乓球运动中需要针对性地对触地模式进行调整和练习,避免使用全脚掌触地模式,提升乒乓球运动者下肢运动能力。

关键词：触地模式乒乓球运动学动力学运动表现

AI 预测天然染料在丝绸上的最佳染色工艺与色牢度研究

在线全文

学校读者我要写书评

暂无评论

丝绸 2025年第1期62卷 40页

作者：刘莹陈杰南宁师范大学美术与设计学院武汉大学物理科学与技术学院

随着环境问题的日益严峻，为了提高实验效率、节约成本，推动可持续发展战略在染料与纺织品领域的应用，文章首先对天然染料染色的数据进行了收集与预处理，然后采用Xgboost集成学习算法构建AI模型并进行了模型训练与验证，评估了模型... 详细信息

随着环境问题的日益严峻，为了提高实验效率、节约成本，推动可持续发展战略在染料与纺织品领域的应用，文章首先对天然染料染色的数据进行了收集与预处理，然后采用Xgboost集成学习算法构建AI模型并进行了模型训练与验证，评估了模型的有效性和预测精度。该模型实现了天然染料在丝绸上染色工艺与效果的高效模拟与预测，从而显著缩短了实验时间并降低了资源损耗。这一方法提高了研究效率和经济效益。研究表明，该模型在预测反应最佳酸碱度(pH值)、水洗色牢度(CFW)和摩擦色牢度(CFR)方面具有较高的准确率，在验证集中三者的预测准确率高达94.12%、93.75%、100%,在真实的测试集上分别拥有87.50%、94.12%、82.35%的准确率，同时具备有效性与可迁移性。集成学习的方法通过极小的部署成本和极短的耗时为天然染料染色性能的探索给出了极具价值的指导，也为AI机器学习与纺织染色的学科的交叉应用拓展了可能性。

关键词： AI预测 Xgboost 天然染料丝绸色牢度

Leader-follower: Human-centered intention-guided controller for novel SuperLimb with application to load-carrying scenarios

在线全文

学校读者我要写书评

暂无评论

Science China Technological Sciences 2025年第1期 325-338页

作者： Qinghua ZHANG Xianglong LI Hongwei JING Kerui SUN Yuancheng LI Sikai ZHAO Lele LI Haotian JU Ziqi WANG Rui WU Tianjiao ZHENG jie ZHAO Yanhe ZHU The State Key Laboratory of Robotics and System Harbin Institute of Technology The Learning Algorithms and Systems Laboratory School of Engineeringécole Polytechnique Fédérale de Lausanne

Supernumerary robotic arms(SuperLimb) are a new type of wearable robot that works closely with humans as a third hand to augment human operation capability. Accurate conveyance of wearers' intentions, allocation of roles, and humancentered interaction considerations are the key points in the process of human–SuperLimb collaboration. This paper proposes a human-centered intention-guided leader–follower controller that relies on the dynamic modeling of SuperLimb with application to load-carrying scenarios. The proposed leader–follower controller takes the human as the leader and the SuperLimb as the follower, achieving effective information communication, autonomous coordination, and good force compliance between SuperLimb, humans, and the environment under human safety assurance. First, the human–SuperLimb dynamic system is modeled to achieve force interaction with the environment and wearer. Second, to achieve the precise intention extraction of humans, pose data from five visual odometry sensors are fused to capture the human state, the generalized position, the velocity of hands, and the surface electromyography signals from two myoelectric bracelets sensors are processed to recognize the natural hand gestures during load-carrying scenarios by a designed Swin transformer network. Then, based on the real-time distance detection between human and mechanical limbs, the security assurance and force-compliant interaction of the human–SuperLimb system are realized. Finally, the human hand muscle intention recognition, human–robot safety strategy verification,and comparative load-carrying experiments with and without the proposed method are conducted on the SuperLimb *** showed that the task parameters are well estimated to produce more reasonable planning trajectories, and SuperLimb could well understand the wearer's intentions to switch different SuperLimb actions. The proposed sensor-based human–robot communication framework motivates future studies of o

关键词：

基于身份的密钥隔离的多云多副本可证数据持有方案

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

计算机科学 2025年第1期52卷 401-411页

作者：周杰王化群南京邮电大学计算机学院南京210023

可证数据持有方案(Provable Data Possession, PDP)可以让用户在不下载全部数据的情况下验证其外包数据是否完好无损。为了提高外包数据的可用性和安全性,许多用户将数据的多个副本存储在单云服务器上,但是单云服务器在发生故障或者其... 详细信息

可证数据持有方案(Provable Data Possession, PDP)可以让用户在不下载全部数据的情况下验证其外包数据是否完好无损。为了提高外包数据的可用性和安全性,许多用户将数据的多个副本存储在单云服务器上,但是单云服务器在发生故障或者其他意外情况时,用户存储的数据副本也会遭到破坏因而无法恢复原始数据。同时,许多可证数据持有方案依赖于公钥基础设施(Public Key Infrastructure, PKI)技术,存在密钥管理问题。此外,现有的可证数据持有方案大多是在用户端使用密钥对数据进行处理。由于用户端的安全意识较弱或者安全设置较低,密钥可能会有泄露的风险。恶意云一旦获得了用户端的密钥,就可以通过伪造虚假的数据持有证明来隐藏数据丢失的事件。基于上述问题,提出了一种基于身份的密钥隔离的多云多副本可证数据持有方案(Identity-Based Key-Insulated Provable Multi-Copy Data Possession in Multi-Cloud Storage, IDKIMC-PDP)。基于身份的可证数据持有方案消除了公钥基础设施技术中复杂的证书管理。多云多副本确保了即使在某个云服务器上的副本被篡改或者被破坏的情况下,用户仍然可以从其他云服务器上获取副本并恢复数据。同时,方案中使用了密钥隔离技术实现了前向和后向安全。即使某一时间段内的密钥泄露,其他时间段内云存储审计的安全性也不会受到影响。给出了该方案的正式定义、系统模型和安全模型;在标准困难问题下,给出了该方案的安全性证明。安全性分析表明,IDKIMC-PDP方案具有强抗密钥泄露性、可检测性以及数据块标签和证明的不可伪造性。实验结果表明,与现有的多云多副本相关方案相比,IDKIMC-PDP方案具有相对较高的效率。

关键词：可证数据持有密钥隔离基于身份的签名多云多副本