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Unraveling the mechanism of action of Shangxia Liangji formula for treating insomnia:a metabolomics and network pharmacology approach

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Traditional Medicine Research 2025年第2期10卷 16-29页

作者： Xia-Jie Quan Hao Liang Yong-Hong Tang Li Jiang Xiong-ying Ji feng-ying zhang Ping zhang Bo Ouyang Department of Information The Affiliated Nanhua HospitalHengyang Medical SchoolUniversity of South ChinaHengyang 421002China Department of Neurology The Affiliated Nanhua HospitalHengyang Medical SchoolUniversity of South ChinaHengyang 421002China Department of Gastroscopy The Affiliated Nanhua HospitalHengyang Medical SchoolUniversity of South ChinaHengyang 421002China Department of Traditional Chinese Medicine The Affiliated Nanhua HospitalHengyang Medical SchoolUniversity of South ChinaHengyang 421002China

Background:Insomnia is a prevalent clinical condition and Shangxia Liangji formula(SXLJF)is a well-established method of ***,the specific mechanism of action of SXLJF remains ***:The mouse model of insomnia was established by intraperitoneal injection of ***-two mice were randomly divided into a negative control group,model group,SXLJF group(18.72 g/kg/day),and positive control group(diazepam,2 mg/kg)and treated with the corresponding drugs for 7 consecutive *** open field test and pentobarbital-induced sleeping test were ***-MS-based untargeted metabolomics and network pharmacology were applied to explore the potential targets of SXLJF for treating ***,key targets were validated using ***:Behavioral tests demonstrated that SXLJF reduced the total distance,average velocity,central distance,and sleep latency,and prolonged sleep *** and network pharmacology revealed potential targets,signaling pathways,metabolic pathways,and metabolites associated with the anti-insomnia effects of ***,tyrosine hydroxylase(TH)and tyrosine metabolism emerged as crucial metabolic pathways and targets,***-qPCR results supported the role of TH in the mechanism of SXLJF in treating ***:In conclusion,TH and tyrosine metabolism may represent significant targets and pathways for SXLJF in treating insomnia.

关键词： Shangxia Liangji formula insomnia metabolomics network pharmacology tyrosine hydroxylase tyrosine metabolism

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Microstructure and corrosion resistance of Fe/Mo composite amorphous coatings prepared by air plasma spraying

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International Journal of Minerals,Metallurgy and Materials 2012年第7期19卷 657-662页

作者： Chao-ping Jiang Ya-zhe Xing feng-ying zhang Jian-min Hao Engineering Research Center of Transportation Materials (Ministry of Education) School of Materials Science and Engineering Chang'an University Xi'an 710064China

Fe/Mo composite coatings were prepared by air plasma spraying （APS） using Fe-based and Mo-based amorphous and nanocrys- talline mixed powders. Microstructural studies show that the composite coatings present a layered structure with low porosity due to adding the self-bonded Mo-based alloy. Corrosion behaviors of the composite coatings, the Fe-based coatings and the Mo-based coatings were in- vestigated by electrochemical measurements and salt spray tests. Electrochemical results show that the composite coatings exhibit a lower polarization current density and higher corrosion potentials than the Fe-based coating when tested in 3.5wt% NaC1 solutions, indicating supe- rior corrosion resistance compared with the Fe-based coating. Also with the increase in addition of the Mo-based alloy, a raised corrosion re- sistance, inferred by an increase in corrosion potential and a decrease in polarization current density, can be found. The results of salt spray tests again show that the corrosion resistance is enhanced by adding the Mo-based alloy, which helps to reduce the porosity of the composite coatings and enhance the stability of the passive films.

关键词： composite coatings plasma spraying amorphous corrosion resistance microstructure

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Wear Resistance and Bond Strength of Plasma Sprayed Fe/Mo Amorphous Coatings

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Journal of Iron and Steel Research International 2014年第10期21卷 969-974页

作者： Chao-ping JIANG Ya-zhe XING feng-ying zhang Jian-min HAO Xu-ding SONG School of Materials Science and Engineering Chang'an University Key Laboratory of Road Construction Technology and Equipment MOE School of Construction MachineryChang'an University

Fe-based and Fe/Mo composite amorphous coatings were deposited on the surface of plain carbon steel substrates by atmospheric plasma spraying （APS）. With increasing the Mo alloy content, the microstructure of the coatings revealed more dense structure. The porosities of composite coating were all less than those of Fe-based coat- ing due to Mo alloy self-bonding performance. The ML10 friction and wear tester was employed to investigate the wear behaviors of the coatings under dry sliding conditions. It was found that the mass loss of the resultant coatings decreased with increasing Mo-based powders into the feedstock. This was attributed to the reduction of the delamina- tions resulting from improved intersplat bond with Mo addition.

关键词： atmospheric plasma spraying coating delamination wear bond strength

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Influence of Trace Amount of Calponin on Smooth Muscle Myosin in Different States

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Acta Biochimica et Biophysica Sinica 2004年第8期36卷 529-536页

作者： Jing-Xian YANG, Xiao-Hua feng, ying zhang, and Yuan LIN Department of Pharmacology, Dalian Medical University, Dalian 116027, China Department of Pharmacology Dalian Medical UniversityDalian 116027China

Calponin (CaP), a thin filament-associated protein, is thought to be involved in modulating smooth muscle contractile activity, but the role and mechanism keep unknown. In this study, trace amount of calponin (TAC) was found to obviously influence myosin in different states in Ca2+-independent manner, suggesting a high efficient interaction between TAC and myosin. In this assay, the lowest ratio of CaP vs. myosin was 1:10,000, with the concentration of CaP 10,000-fold lower than that used previously. Myosin phosphorylation, myosin Mg2+-ATPase activity and protein binding activity were detected to determine the effects of TAC on the myosin in different states. The amount of precipitated myosin that bound to TAC was used as the index to determine the interaction between myosin and TAC in binding assay. Our data showed that in the absence of actin, TAC significantly increased the precipitation of unphosphorylated myosin, Ca2+- dependently or independently phosphorylated myosin by MLCK, and stimulated the Mg2+-ATPase activities of these myosins slightly but significantly. However, no obvious change of precipitation of myosin phospho- rylated by PKA was observed, indicating the relatively selective effect of TAC. In the presence of actin, the increase of myosin precipitations was abolished, and no obvious change of actin precipitations and actin- activated myosin Mg2+-ATPase activities were observed implicating the high efficiency of TAC on myosin being present in the absence of actin. Although we can not give conclusive comments to our results, we propose that the high efficiency of TAC-myosin interaction is present when actin is dissociated from myosin, even if CaP/myosin ratio is very low; this high efficient interaction can be abolished by actin. However, why and how TAC can possess such a high efficiency to influence myosin and how the physiological significance of the high efficiency of TAC is in regulating the interaction between myosin and actin remain to be inve

关键词： trace amount of calponin protein binding phosphorylation myosin Mg2+-ATPase

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AT1 receptor in rostral ventrolateral medulla mediating blunted baroreceptor reflex in spontaneously hypertensive rats

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Acta Pharmacologica Sinica 2004年第11期 49-54页

作者： Xin-ya GAO, feng zhang, ying HAN, Han-jun WANG, ying zhang, Rui GUO, Guo-qing ZHUDepartment of Physiology, Nanjing Medical University, Nanjing 210029, China

AIM: To determine the role of AT 1 receptor in the rostral ventrolateral medulla (RVLM) in mediating the blunted baroreceptor reflex in spontaneously hypertensive rats (SHR). METHODS: Intravenous injections of graded doses of phenylephrine (1, 5, 10, 20, and 40 μg/kg) increased the blood pressure to elicit the baroreceptor reflex in both SHR and normotensive Wistar rats anesthetized with urethane. The baroreceptor reflex sensitivity (BRS) was determined before and after microinjection of Ang Ⅱ, losartan, or AT1 receptor antisense oligodeoxynucleotides into the RVLM. AT1 receptor protein level in the RVLM was measured by Western blotting. RESULTS: The BRS was significantly decreased in SHR compared with normal rats. Bilateral microinjection of AT1 receptor antagonist losartan (250 nmol/h) into the RVLM partly reversed the blunted BRS in SHR, but had no significant effect on the BRS in normal rats. Ang Ⅱ (1.5 nmol/h) significantly inhibited the BRS in normal rats, which was completely abolished by pretr

关键词： angiotensin Ⅱ antisense oligodeoxynucleotide hypertension baroreceptor reflex rostral ventrolat-eral medulla

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Exercise test on the patients with normokalaemic periodic paralysis from a Chinese family with a mutation in the SCN4A gene

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Chinese Medical Journal 2008年第19期121卷 1915-1919页

作者： feng Yu zhang ying LIU Zhong-lan zhang Chao-dong Department of Neurology First Affiliated Hospital of ChinaMedical University Shenyang Liaoning 110001 China

Background Normokalaemic periodic paralysis （normoKPP） is characterized by transient and recurrent myoasthenia, and some patients also show muscle stiffness induced by cold exposure （paramyotonia congenita, PMC）. It is caused by a mutation in the muscle voltage gated sodium channel alpha subunit （SCN4A） gene. Due to the diversity of the clinical manifestations of patients, it is difficult for clinicians to differentiate some of patients with atypical normoKPP from those who suffer from other periodic paralysis and nondystrophic myotonia. So far, for normoKPP there are almost no ways to assist definite diagnosis besides genetic screening. This research was designed to evaluate an exercise test （ET） in confirming the diagnosis of normoKPP and in assessing the therapeutic effectiveness of some drugs on this disease. Methods ET, described by McMains, was performed on six subjects from a Chinese family, including four patients with overlapping disease of normoKPP and PMC caused by a mutation of SCN4A Met1592Val that is identified by genetic analysis and two normal control members. The change of compound muscle action potential （CMAP） was recorded. Besides the family, two patients were also tested during treatments with acetazolamide. Results All patients showed a slight increase in CMAP immediately after exercise, followed by an abnormal gradual decline, which reached its nadir 25-30 minutes after exercise. CMAP amplitude dropped by more than 40% in patients but less than 23% in controls. In the patients who received treatment with acetazolamide, the change of CMAP amplitude was less than 28% and, at any fixed times, less than pretreatment values. Conclusions The ET may be used as a predictive, easy and reliable method of diagnosing normoKPP under conditions without genetic screening help, and is an objective way to evaluate the therapeutic effectiveness. According to different response patterns, the ET may also be helpful in reducing the scope of genetic screening.

关键词： exercise test normokalaemic periodic paralysis paramyotonia congenita,skeletal muscle sodium channelopathy

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Interventional effect of hirudin on the expression of microtubule-associated protein 2 in peripheral tissue of hematom of model rats with acute intracerebral hemorrhage

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Neural Regeneration Research 2006年第3期1卷 230-233页

作者： Jiachun feng ying zhang Fang Deng Department of Neurology First Affiliated Hospital of Jilin University Changchun 130021 Jilin Province China

BACKGROUND： It is suspected that dissociation, destruction or synthetic disorder of microtubule-associated protein 2 （MAP-2） may participate in secondary injury of intracerebral hemorrhage （ICH）, and the reason may be related to thrombin in high concentration after ICH; therefore, the mechanism should be studied further. OBJECTIVE： To explore the effect of hirudin on expression of MAP-2 in peripheral tissue of hematom after ICH and changes of water content in brain tissue and analyze pathogenesis of thrombin in secondary injury after ICH. DESIGN ： Completely randomized grouping design and controlled animal study SEn-ING ： Department of Neurology, the First Affiliated Hospital of Jilin University MATERIALS ： The experiment was carried out in the Neurological Laboratory of the First Affiliated Hospital of Jilin University from April 2003 to April 2004. A number of 80 healthy Wistar rats, of both genders, aged 3-4 months, weighing 250-350 g, were randomly divided into 8 groups： normal control group, 6-hour ICH group, 1-day ICH group, 2-day ICH group, 3-day ICH group, 7-day ICH group, 3-day hirudin group and 7-day hirudin group with 10 in each group. Five rats from each group were selected to measure their water content, and the others were undertaken immunohistochemical stain. Hirudin was produced by Sigma Company, USA, and MAP-2 rabbit-rat polyclonal antibody was provided by Fuzhou Maixin Biotechnology Company Limited. METHODS： ① Model establishing and grouping intervention： Rats in simple ICH group were collected their blood from tails and then inserted with 50 μL non-anticoagulant auto-arterial blood into the cauda of the putamen in right brain within 5 minutes. Rats in hirudin groups were inserted with 10 U hirudin （which was diluted with saline to 20 μL） into local hematom regions within 5 minutes, and the needle was pulled out after 10 minutes. Rats in normal control group were untouched. ② Water content in peripheral tissue of hematom： Based on the ratio between

关键词： ICH Interventional effect of hirudin on the expression of microtubule-associated protein 2 in peripheral tissue of hematom of model

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Diversity Analysis of Paenibacillus Cellulase

Diversity Analysis of Paenibacillus Cellulase

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作者： Ming SUI feng-ying zhang Jun-ru LI Department of Wine and Food Engineering Sichuan Technology and Business College Key Laboratory of Leather Chemistry and Engineering Ministry of Education and College of Light IndustryTextile & Food EngineeringSichuan University

Cellulose is one of the most abundant biomass resources on the *** degradation of cellulose into bio-available sugar is of great significance to the development of bio-energy and bio-based chemicals as well as the comprehensive utilization of biomass resources.A large part of the cellulose degradation method is the use of cellulase *** includes exo-β-glucanase,β-glucosidase,endo-β-glucanase and the *** general,cellulase produced by microorganisms in nature belongs to an inducible enzyme,and different substrates may induce a variety of *** this paper,we focused on the laboratory-screened ***1,and explored the changes of cellulase activity when the strain was cultured under different carbon sources,revealing the cellulase diversity produced by different carbon *** experimental results showed that when using sorbose and cellobiose as carbon sources,cellulase showed the highest activity of enzyme activity and CMC-Naenzyme ***-PAGE analysis showed that LLZ1 cellulase possessed the natural diversity and could be induced by different substrates Produce different β-glucanase enzymes.

关键词： cellulase induction enzyme activity gel electrophoresis

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Is there any difference in anesthetic management of different post-OLT stage patients undergoing nontransplant organ surgery?

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Hepatobiliary & Pancreatic Diseases International 2006年第3期5卷 368-373页

作者： Zhi-ying feng, Jian zhang, Sheng-Mei Zhu and Shu-Sen Zheng Department of Anesthesiology, and Department of General Surgery First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China Department of Anesthesiology First Affiliated Hospital Zhejiang University School of Medicine Hangzhou 310003 China.

BACKGROUND: Little information is available about anesthesia management of nontransplant organ surgery of recipients after adult liver transplantation. The aim of this study was to discuss the anesthesia management of recipients for different stages after liver transplantation. METHODS: The medical records of 16 patients were reviewed after OLT scheduled for elective nontransplant organ surgery at our institution from September 2002 to October 2005. The patients were divided into perioperative stage (group A) and mid-term and long-term stage (group B) groups according to post-OLT time. The data of 16 patients preoperation, intraoperation and postoperation were analyzed. RESULTS: The measurements of alanine transaminase (ALT), total bilirubin (TB), prothrombin time (PT), and lung infection were significantly higher in group A than in group B (P<0.05). The incidence of hyperglycaemia was significantly higher in group B than in group A (P<0.05). During operation the incidence of hypotension was significantly higher in group A than in group B (P<0.05). After operation, the number of patients in ICU was significantly larger and the extubation time was longer in group A than in group B. General anesthesia was induced in 14 patients, and regional anesthesia in 2 patients. CONCLUSIONS: Regional or general anesthesia can be safely delivered to adult OLT recipients except for contraindications. Special considerations include protection of the function of important organs, correction of hemodynamic instability in perioperative stage patients after OLT, and measurement of the side-effects of immunosuppression in mid-term and long-term stage patients.

关键词： liver transplantation reoperation anesthetic management

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新疆旅游经济空间分异及影响因素

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地域研究与开发 2017年第4期36卷 99-104页

作者：冯迎张军民郑州成功财经学院商学系郑州451200 石河子大学政法学院新疆石河子832000 石河子大学理学院新疆石河子832000

根据空间经济学理论,应用GIS技术和多元回归方法,研究2003—2013年新疆旅游经济空间分异格局,探究影响空间分异的主导因素。结果表明:新疆旅游经济形成了"伊宁—乌鲁木齐—库尔勒—阿克苏"核心环线辐射和以阿勒泰、喀什为两翼的"轴-点... 详细信息

根据空间经济学理论,应用GIS技术和多元回归方法,研究2003—2013年新疆旅游经济空间分异格局,探究影响空间分异的主导因素。结果表明:新疆旅游经济形成了"伊宁—乌鲁木齐—库尔勒—阿克苏"核心环线辐射和以阿勒泰、喀什为两翼的"轴-点双核极化"空间格局;同时,沿极化点-轴方向向周边迅速递减。热点集聚增长迅速,冷点长期增长乏力,但变化范围都不大;Logistic回归分析显示,新疆旅游经济发展水平与旅游基础设施呈正相关演变,同时受地域自然环境及社会经济环境的综合制约。

关键词：旅游经济空间演变影响因素空间统计分析新疆

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