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Chromosome-level assembly of triploid genome of Sichuan pepper(Zanthoxylum armatum)

Horticultural Plant Journal 2024年第2期10卷 437-449页

作者： Lizhi Song Yue Huang Hao Zuo Ning Tang Zhengguo Li Wenbiao Jiao feng Xu Qiang Xu Zexiong Chen National k.y Laboratory for Germplasm Innovation and Utilization for Fruit and Vegetable Horticultural Crops Huazhong Agricultural UniversityWuhanHubei 430070China Chongqing k.y Laboratory of Economic Plant Biotechnology Chongqing University of Arts and SciencesChongqing 402160China School of Life Sciences Chongqing UniversityChongqing 400030China College of Horticulture and Gardening Yangtze UniversityJingzhouHubei 434025China College of Informatics Huazhong Agricultural UniversityWuhanHubei 430070China

As an important spice species in Rutaceae, the Sichuan pepper (Zanthoxylum armatum) can provide pungent and numbing taste, as well as aroma in its mature fruit. Here we assembled a chromosome-level genome of green prick.y ash which was widely cultivated in a major production area including Chongqing and Sichuan province, China. We generated 712 Gb (~112×) PacBio long reads and 511 Gb (~82×) Hi-C data, and yielded an assembly of 99 pseudochromosomes with total size of 5.32 Gb and contig N50 of 796 k.. The genomic analyses and cytogenetic experiments both indicated that the cultivarZhuye Huajiao’ was a triploid. We identified a Zanthoxylum-specific whole genome duplication event emerging about 24.8 million years ago (Mya). We also detected a transposition burst event (0.3-0.4 Mya) responsible for the large genome size of Z. armatum. Metabolomic analysis of the Zanthoxylum fruits during development stages revealed profiles of39 volatile aroma compounds and 528 secondary metabolites, from which six types of sanshools were identified. Based on metabolomic and transcriptomic network.analysis, we screened candidate genes encoding long chain acyl-CoA synthetase, fatty acid desaturase,branched-chain amino acid aminotransferase involved in sanshool biosynthesis and three genes encoding terpene synthase during fruit development. The multi-omics data provide insights into the evolution of Zanthoxylum and molecular basis of numbing and aroma flavor of Sichuan pepper.

关键词： Zanthoxylum armatum Genome Sanshool Terpenoid

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MoS_(x) nanowire network. derived from[Mo_(3)S_(13)]^(2−) clusters for efficient electrocatalytic hydrogen evolution

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Nano Research 2024年第8期17卷 6910-6915页

作者： Haoxuan Yu Junan Pan k.ng Chen Wang Chao Zechao Zhuang Sizhuo feng Jianmei Chen Lingbin Xie Longlu Wang Qiang Zhao College of Electronic and Optical Engineering&College of Flexible Electronics(Future Technology) Nanjing University of Posts and Telecommunications9 Wenyuan RoadNanjing 210023China Department of Chemical Engineering Columbia UniversityNew YorkNY 10027USA Institute of Advanced Materials(IAM)&Institute of Flexible Electronics(Future Technology) Nanjing University of Posts and Telecommunications9 Wenyuan RoadNanjing 210023China

Precise design and synthesis of sub-nano scale catalysts with controllable electronic and geometric structures are pivotal for enhancing the hydrogen evolution reaction(HER)performance of molybdenum sulfide(MoS_(2))and unraveling its structure−activity *** leveraging transition molybdenum polysulfide clusters as functional units for multi-level ordering,we successfully designed and synthesized MoS_(x)nanowire network. derived from[Mo_(3)S_(13)]^(2−) clusters via evaporationinduced self-assembly,which exhibit enhanced HER activity attributed to a high density of active sites and dynamic evolution behavior under cathodic ***_(x) nanowire network. electrode yields a current density of 100 mA·cm^(−2) at 142 mV in 0.5 M H_(2)SO_(4).This work.provides an attractive prospect for optimizing catalysts at the sub-nano scale and offers insights into a strategy for designing catalysts in various gas evolution reactions.

关键词： MoS_(x) nanowire network. [Mo_(3)S_(13)]^(2−)clusters hydrogen evolution reaction(HER) sub-nano scale

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Atomistic Investigation of Shock.Induced Amorphization within Micro-shear Bands in Hexagonal Close-Pack.d Titanium

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Acta Metallurgica Sinica(English Letters) 2024年第9期37卷 1590-1600页

作者： Z.C.Meng k.G.Wang T.Ali D.Li C.G.Bai D.S.Xu S.J.Li A.H.feng G.J.Cao J.H.Yao Q.B.Fan H.Wang R.Yang Institute of Metal Research Chinese Academy of SciencesShenyang 110016China School of Materials Science and Engineering University of Science and Technology of ChinaShenyang 110016China Interdisciplinary Centre for Additive Manufacturing(ICAM) School of Materials and ChemistryUniversity of Shanghai for Science and TechnologyShanghai 200093China School of Materials Science and Engineering Tongji UniversityShanghai 201804China k.y Laboratory for Light-Weight Materials Nanjing Tech UniversityNanjing 210009China National k.y Laboratory of Science and Technology on Materials Under Shock.and Impact School of Materials Science and EngineeringBeijing Institute of TechnologyBeijing 100081China Beijing Institute of Technology Chongqing Innovation Center Chongqing 401135China

The mechanical response of a single crystal titanium sample against(0001)α surface impact was investigated using molecular dynamics ***,non-uniform plastic deformation was observed in the *** high strain rates,amorphization occurred near the edge of the contact region where severe shear strain induced a large number of stack.ng faults(SFs)and *** contrast,the central part of the contact region underwent less deformation with significantly fewer ***,instead of amorphization by consuming SFs and dislocations,there was a gradual increase in the density of dislocations and SFs during the process of *** local amorphous regions eventually grew into shear bands.

关键词： Shock.compression Amorphization Shear band Titanium Molecular dynamics

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In vivo real-time monitoring delayed administration of M2 macrophages to enhance healing of tendon by NIR-II fluorescence imaging

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Nano Research 2024年第5期17卷 4379-4390页

作者： Yuzhou Chen Mo Chen Chengxuan Yu Huizhu Li Liman Sai Nguyen T.k.Thanh Yueming Wang Yan Wo Jian Zhang Xing Yang Evgenii L.Guryev Andrei V.Zvyagin Hao De Min Tang Shiyi Chen Yunxia Li Yuefeng Hao Sijia feng Jun Chen Sports Medicine Institute of Fudan University Department of Sports MedicineHuashan HospitalFudan UniversityShanghai 200040China Department of Orthopedic Surgery Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of MedicineShanghai 200092China Department of Bone and Joint Surgery Department of OrthopedicsRenji HospitalSchool of MedicineShanghai Jiao Tong UniversityShanghai 200001China Department of Physics Shanghai Normal UniversityShanghai 200234China Biophysics Group Department of Physics and AstronomyUniversity College LondonLondon WC1E 6BTUK UCL Healthcare Biomagnetic and Nanomaterials Laboratories London W1S 4BSUK Department of Anatomy and Physiology School of MedicineShanghai Jiao Tong UniversityShanghai 200025China Department of Orthopedics Affiliated Suzhou Hospital of Nanjing Medical UniversitySuzhou 215500China Institute of Biology and Biomedicine Lobachevsky State University of Nizhny NovgorodNizhny Novgorod 603950Russia Mathematical Institute University of OxfordOxford OX26GGUK Institute of Natural Sciences School of MathematicsShanghai Jiao Tong UniversityShanghai 200240China

The administration time is a critical but long-neglected point in cell therapy based on macrophages because the incorrect time of macrophage administration could result in diverse outcomes regarding the same macrophage *** this work.the second near-infrared(NIR-II)fluorescence imaging in vivo track.ng of M2 macrophages during a pro-healing therapy in the mice model of rotator cuff injury revealed that the behavior of administrated macrophages was influenced by the timing of their *** delayed cell therapy(DCT)group had a longer retention time of injected M2 macrophages in the repairing tissue than that in the immediate cell therapy(ICT)*** k.ller-Segel model and histological analysis further demonstrated that DCT altered the chemotaxis of M2 macrophages and improved the healing outcome of the repaired structure in comparison with *** results offer a possible explanation of previous conflicting results on reparative cell therapy and provok. reconsideration of the timing of these therapies.

关键词： macrophage cell therapy NIR-II in vivo imaging delayed therapy tendon healing

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Two-photon polymerization lithography for imaging optics

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International Journal of Extreme Manufacturing 2024年第4期6卷 21-60页

作者： Hao Wang Cheng-feng Pan Chi Li k.shan S Menghrajani Mark.s A Schmidt Aoling Li Fu Fan Yu Zhou Wang Zhang Hongtao Wang Parvathi Nair Suseela Nair John You En Chan Tomohiro Mori Yueqiang Hu Guangwei Hu Stefan A Maier Haoran Ren Huigao Duan Joel k.W Yang College of Mechanical and Vehicle Engineering Hunan UniversityChangsha 410082People's Republic of China Engineering Product Development Singapore University of Technology and DesignSingapore 487372Singapore Greater Bay Area Institute for Innovation Hunan UniversityGuangzhou 511300People's Republic of China Department of Electrical and Computer Engineering National University of Singapore4 Engineering Drive 3Singapore 117576Singapore School of Physics and Astronomy Faculty of ScienceMonash UniversityMelbourneVictoria 3800Australia Leibniz Institute of Photonic Technology 07745 JenaGermany Abbe Center of Photonics and Faculty of Physics FSU Jena07745 JenaGermany Otto Schott Institute of Material Research FSU Jena07745 JenaGermany Institute of Materials Research and Engineering A*STAR(Agency for Science Technology and Research)Singapore 138634Singapore Industrial Technology Center of Wak.yama Prefecture Wakayama 6496261Japan School of Electrical and Electronic Engineering Nanyang Technological UniversitySingapore639798Singapore Department of Physics Imperial College LondonLondon SW72AZUnited Kingdom

Optical imaging systems have greatly extended human visual capabilities,enabling the observation and understanding of diverse *** technologies span a broad spectrum of wavelengths from x-ray to radio frequencies and impact research activities and our daily *** glass lenses are fabricated through a series of complex processes,while polymers offer versatility and ease of ***,modern applications often require complex lens assemblies,driving the need for miniaturization and advanced designs with micro-and nanoscale features to surpass the capabilities of traditional fabrication ***-dimensional(3D)printing,or additive manufacturing,presents a solution to these challenges with benefits of rapid prototyping,customized geometries,and efficient production,particularly suited for miniaturized optical imaging *** 3D printing methods have demonstrated advantages over traditional counterparts,yet challenges remain in achieving nanoscale ***-photon polymerization lithography(TPL),a nanoscale 3D printing technique,enables the fabrication of intricate structures beyond the optical diffraction limit via the nonlinear process of two-photon absorption within liquid *** offers unprecedented abilities,***-free fabrication,micro-and nanoscale capabilities,and rapid prototyping of almost arbitrary complex 3D *** this review,we emphasize the importance of the criteria for optical performance evaluation of imaging devices,discuss material properties relevant to TPL,fabrication techniques,and highlight the application of TPL in optical *** the first panoramic review on this topic,it will equip researchers with foundational k.owledge and recent advancements of TPL for imaging optics,promoting a deeper understanding of the *** leveraging on its high-resolution capability,extensive material range,and true 3D processing,alongside advances in materials,fabrication,and design,we en

关键词： two-photon polymerization lithography 3D printing additive manufacturing imaging optics and nanophotonics

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A hybrid CNN-LSTM model for diagnosing rice nutrient levels at the rice panicle initiation stage

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Journal of Integrative Agriculture 2024年第2期23卷 711-723页

作者： Fubing Liao Xiangqian feng Ziqiu Li Danying Wang Chunmei Xu Guang Chu Hengyu Ma Qing Yao Song Chen School of Computer Science and Technology Zhejiang Sci-Tech UniversityHangzhou 310018China China National Rice Research Institute Chinese Academy of Agricultural SciencesHangzhou 310006China School of Agriculture Yangtze UniversityJingzhou 434025China

Nitrogen(N)and potassium(k.are two k.y mineral nutrient elements involved in rice *** diagnosis of N and k.status is very important for the rational application of fertilizers at a specific rice growth ***,we propose a hybrid model for diagnosing rice nutrient levels at the early panicle initiation stage(EPIS),which combines a convolutional neural network.CNN)with an attention mechanism and a long short-term memory network.LSTM).The model was validated on a large set of sequential images collected by an unmanned aerial vehicle(UAV)from rice canopies at different growth stages during a two-year *** with VGG16,AlexNet,GoogleNet,DenseNet,and inceptionV3,ResNet101 combined with LSTM obtained the highest average accuracy of 83.81%on the dataset of Huanghuazhan(HHZ,an indica cultivar).When tested on the datasets of HHZ and Xiushui 134(XS134,a japonica rice variety)in 2021,the ResNet101-LSTM model enhanced with the squeeze-and-excitation(SE)block.achieved the highest accuracies of 85.38 and 88.38%,*** the cross-dataset method,the average accuracies on the HHZ and XS134 datasets tested in 2022 were 81.25 and 82.50%,respectively,showing a good *** proposed model work. with the dynamic information of different rice growth stages and can efficiently diagnose different rice nutrient status levels at EPIS,which are helpful for mak.ng practical decisions regarding rational fertilization treatments at the panicle initiation stage.

关键词： dynamic model of deep learning UAV rice panicle initiation nutrient level diagnosis image classification

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Banxia Xiexin Decoction Alleviated Cerebral Glucose Metabolism Disorder by Regulating Intestinal Microbiota in APP/PS1 Mice

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Chinese Journal of Integrative Medicine 2024年第8期30卷 701-712页

作者： GAO Chen-yan QIN Gao-feng ZHENG Ming-cui TIAN Mei-jing HE Yan-nan WANG Peng-wen k.y Laboratory of Chinese Internal Medicine of Ministry of Education and Beijing Dongzhimen HospitalBeijing University of Chinese MedicineBeijing100700China Neurology Department Binzhou Medical University HospitalBinzhouShandong Province256603China

Objective To identify whether Banxia Xiexin Decoction(BXD)alleviates cerebral glucose metabolism disorder by intestinal microbiota regulation in APP/PS1 *** Forty-five 3-month-old male APP/PS1 mice were divided into 3 groups using a random number table(n=15 per group),including a model group(MG),a liraglutide group(LG)and a BXD group(BG).Fifteen 3-month-old male C57BL/6J wild-type mice were used as the control group(CG).Mice in the BG were administered BXD granules by gavage at a dose of 6 g/(k.·d)for 3 months,while mice in the LG were injected intraperitoneally once daily with Liraglutide Injection(25 nmol/k.)for 3 ***,liquid chromatography with tandem-mass spectrometry was used to analyze the active components of BXD granules and the medicated serum of ***,the cognitive deficits,Aβpathological change and synaptic plasticity mark.rs,including synaptophysin(SYP)and postsynaptic density protein 95(PSD95),were measured in APP/PS1 *** glucose uptak. was detected by micropositron emission *** microbial constituents were detected by 16S rRNA *** levels of intestinal glucagon-lik. peptide 1(GLP-1)and cerebral GLP-1 receptor(GLP-1R),as well as the phosphoinositide-3-k.nase/protein k.nase B/glycogen synthase k.nase-3β(PI3k.Ak./GSk.β)insulin signaling pathway were determined by immunohistochemical(IHC)staining and Western blot analysis,*** BXD ameliorated cognitive deficits and Aβpathological features(Pk. in the BG was higher than that in the MG(Pk.,PI3k.and p-PI3k.in the BG were significantly increased(Pk.βwere reduced(P<0.01).Conclusion BXD

关键词： Banxia Xiexin Decoction Alzheimer's disease intestinal microbiota glucagon-lik. peptide 1 cerebral insulin resistance phosphoinositide-3-k.nase/proteink.nase B/glycogen synthase k.nase-3β Chinese medicine

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A virtual thermometer for ultrahigh-temperature-pressure experiments in a large-volume press

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Matter and Radiation at Extremes 2024年第4期9卷 98-110页

作者： Bingtao feng Longjian Xie Xuyuan Hou Shucheng Liu Luyao Chen Xinyu Zhao Chenyi Li Qiang Zhou k.o Hu Zhaodong Liu Bingbing Liu State k.y Laboratory of Superhard Materials Synergetic Extreme Condition User FacilityCollege of PhysicsJilin UniversityChangchun 130012China Department of Earth Sciences University College LondonLondon WC1E 6BSUnited Kingdom Earth and Planetary Laboratory Carnegie Institute for ScienceWashingtonDistrict of Columbia 20015USA College of Earth Sciences Jilin UniversityChangchun 130012China

Ultrahigh-temperature-pressure experiments are crucial for understanding the physical and chemical properties of *** recent development of boron-doped diamond(BDD)heaters has made such melting experiments possible in large-volume ***,estimates of temperatures above 2600 k.and of the temperature distributions inside BDD heaters are not well constrained,owing to the lack.of a suitable ***,we establish a three-dimensional finite element model as a virtual thermometer to estimate the temperature and temperature field above 2600 *** advantage of this virtual thermometer over those proposed in previous studies is that it considers both alternating and direct current heating modes,the actual sizes of cell assemblies after compression,the effects of the electrode,thermocouple and anvil,and the heat dissipation by the pressure-transmitting *** virtual thermometer reproduces the power-temperature relationships of ultrahigh-temperature-pressure experiments below 2600 k.at press loads of 2.8-7.9 MN(~19 to 28 GPa)within experimental *** temperatures above 2600 k.predicted by our virtual thermometer are within the uncertainty of those extrapolated from power-temperature relationships below 2600 ***,our model shows that the temperature distribution inside a BDD heater(19-26 k.mm along the radial direction andk.mm along the longitudinal direction)is more homogeneous than those inside conventional heaters such as graphite or LaCrO_(3) heaters(100-200 k.mm).Our study thus provides a reliable virtual thermometer for ultrahigh-temperature experiments using BDD heaters in Earth and material sciences.

关键词： virtual ultrahigh sizes

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Three Types of Isocoumarins with Unusual Carbon Sk.letons from Artemisia dubia var. subdigitata and Their Antihepatoma Activity

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Chinese Journal of Chemistry 2024年第16期42卷 1901-1912页

作者： k.-Xin Yang Tian-Ze Li Yun-Bao Ma Yong-Cui Wang feng-Jiao Li Ji-Jun Chen State k.y Laboratory of Phytochemistry and Plant Resources in West China Kunming Institute of BotanyChinese Academy of SciencesKunmingYunnan 650201China University of Chinese Academy of Sciences Beijing 100049China

Ten novel isocoumarins,including four pairs of enantiomers,were isolated from Artemisia dubia ***(Asteraceae).Compounds 1,2 and 3a/3b possessed a unique 6/6/6-tricyclic system comprising an unusual 1-(2-methylcyclohexyl)propan-1-one moiety fused with isocoumarin core *** 4a/4b were characterized as an unexpected 2,5-dimethylcyclohexan-1-one scaffold,and compounds 5a/5b and 6a/6b were rare 1,*** structures and absolute configurations were elucidated through spectroscopic data,X-ray crystallography,ECD and NMR calculations with DP4+*** biosynthetic pathways were proposed from the naturally occurring *** pharmacological analysis suggested that the targets of compound 1 were significantly enriched in the cell cycle and Pl3k.Ak. signaling *** molecular dock.ng revealed that compound 1 had high binding affinity with CDk.(total score:6.8717).Furthermore,compounds 1 and 2 exhibited inhibitory activity on three human hepatoma cell lines,with inhibitory ratios of 85.1% and 84.5%(HepG2),88.2% and 87.3%(Huh7),and 69.2% and 69.1%(Sk.Hep-1)at 200μmol·L^(-1),respectively.

关键词： Artemisia dubia var.subdigitata Isocoumarins Antihepatoma activity Cancer Natural products X-ray diffraction

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Mechanism of k.Ni Etching for Biochar-H_(2)O Gasification

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Journal of Harbin Institute of Technology(New Series) 2024年第3期31卷 1-18页

作者： Zhenyu Cheng Dongdong feng Qi Shang Yijun Zhao Wenda Zhang Shaozeng Sun School of Energy Science and Engineering Harbin Institute of TechnologyHarbin 150001China

Biomass-H_(2)O gasification is a complex thermochemical reaction,including three processes of volatile removal:homogeneous/heterogeneous reforming,biochar gasification and *** rate-determining step is biochar-H_(2)O gasification and etching so the DFT is carried out to see the catalytic role of different metal elements(k.Ni)in the zigzag biochar *** calculation results show that the gasification of biochar-H_(2)O needs to go through four processes:dissociative adsorption of water,hydrogen transfer(hydrogen desorption,hydrogen atom transfer),carbon dissolution and CO *** energy barrier indicated that the most significant step in reducing the activation energy of k.is reflected in the hydrogen transfer step,which is reduced from 374.14 k./mol to 152.41 k./mol;the catalytic effect of Ni is mainly reflected in the carbon dissolution step,which is reduced from 122.34 k./mol to 84.8 k./*** existence of k.causes the edge to have a stronger attraction to H and does not destroy theπbonds of biochar *** destruction ofπbonds is mainly due to the role of H free radicals,while the destruction ofπbonds will lead to easier C-C bond *** shows a strong attraction to O in OH,which forms strong Ni-O chemical *** can also destroy the aromatic structure directly,mak.ng the gasification easier to *** study explored the catalytic mechanism of k.Ni on the biochar-H_(2)O gasification at the molecular level and look.d forward to the potential synergy of k.Ni,laying a foundation for experimental research and catalyst design.

关键词： Biochar Potassium-nick.l catalysis H_(2)O gasification etching Quantum chemistry Transition state theory

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