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Increased frequency and clinical significance of myeloidderived suppressor cells in human colorectal carcinoma

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World Journal of Gastroenterology 2012年第25期18卷 3303-3309页

作者： hong-Li Sun, Xin Zhou, Yi-feng Xue Ke Wang, Yun-feng ShenI Jing-Jue Mao hong-feng Guo Zong-Ning Miao Department of Hematology Wuxi People'sHospitalWuxi 214023 JiangProvlnce China Department of General Surgery Wuxi Third People'sHospital.Wuxi 214023 Jiangsu Province China The Stem Cell Research Laboratory WuxiThird People's Hospital Wuxi 214023 Jiangsu Province China Department of Clinical Laboratory Wuxi People'sHospital Wuxi 214023 Jiangsu Province China

AIM： To investigate the frequency and clinical signifi- cance of the myeloid-derived suppressor cells （MDSC） in human colorectal carcinoma （CRC）. METHODS： Samples of peripheral blood and tumor tis- sue from 49 CRC patients were analyzed. Mononuclear cells were isolated by FicolI-Hypaque density gradient centrifugation and were subjected to a flow cytometry- based immunophenotypic analysis. RESULTS： A considerable increase in the percentage of CD33＋HLA-DR MDSCs was observed in the periph- eral blood （1.89% ：1= 0.75%） and tumor tissues （2.99%±1.29%） of CRC patients as compared with that in theperipheral blood of healthy controls （0.54%±0.35%）. This expanded CD33＋HLA-DR subset exhibited imma- ture myeloid cell markers, but not lineage markers, and showed up-regulation of CD18/CD11b expression as compared with the MDSCs from healthy donors. Fur- ther studies showed that the MDSC proportion in CRC peripheral blood was correlated with nodal metastasis （P = 0.023）, whereas that in tumor tissues was cor- related with nodal/distant metastasis （P = 0.016/P = 0.047） and tumor stage （P = 0.028）, suggesting the involvement of MDSCs in CRC tumor development. CONCLUSION： Characterization of MDSCs in CRC sug- gests the clinical significance of circulating and tumor- infiltrating MDSCs and may provide new insights into the CRC immunotherapy targeting MDSCs.

关键词： Myeloid-derived suppressor cell Colorectalcarcinoma Tumor metastasis

COMPOSITE ELEMENT MODEL FOR ROCK MASS SEEPAGE FLOW

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Journal of Hydrodynamics 2006年第2期18卷 219-224页

作者： CHEN Sheng-hong feng Xue-min State Key Laboratory of Water Resources and Hydropower Engineering Science Wuhan UniversityWuhan 430072 China

In this paper, the algorithm developed on the concept of the Composite Element Method （CEM） is proposed for the seepage flow in discontinuous rock masses, The basic idea is that if an element contains one or more discontinuity segments, it will be denoted as a composite element having a set of hydraulic potential values defined at its nodes. By the variation principle, the governing equation can be established to solve the unknown hydraulic potential values at the composite element nodes. The main advantage of the proposed model is that the generation of calculation mesh will not be restricted strongly by the number, the position and the orientation of the discontinuities, A numerical example is presented at the end of the paper, to show the validity of the proposed model.

关键词： Composite Element Method(CEM) rock mass seepage flow

FVID: Fishing Vessel Type Identification Based on VMS Trajectories

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Journal of Ocean University of China 2019年第2期18卷 403-412页

作者： HUANG Haiguang hong feng LIU Jing LIU Chao feng Yuan GUO Zhongwen College of Information Science and Engineering Ocean University of China Wenzhou Ocean and Fishery Vessel Safety Rescue Information Center

Vessel Monitoring System(VMS) provides a new opportunity for quantified fishing research. Many approaches have been proposed to recognize fishing activities with VMS trajectories based on the types of fishing vessels. However, one research problem is still calling for solutions, how to identify the fishing vessel type based on only VMS trajectories. This problem is important because it requires the fishing vessel type as a preliminary to recognize fishing activities from VMS trajectories. This paper proposes fishing vessel type identification scheme(FVID) based only on VMS trajectories. FVID exploits feature engineering and machine learning schemes of XGBoost as its two key blocks and classifies fishing vessels into nine types. The dataset contains all the fishing vessel trajectories in the East China Sea in March 2017, including 10031 pre-registered fishing vessels and 1350 unregistered vessels of unknown types. In order to verify type identification accuracy, we first conduct a 4-fold cross-validation on the trajectories of registered fishing vessels. The classification accuracy is 95.42%. We then apply FVID to the unregistered fishing vessels to identify their types. After classifying the unregistered fishing vessel types, their fishing activities are further recognized based upon their types. At last, we calculate and compare the fishing density distribution in the East China Sea before and after applying the unregistered fishing vessels, confirming the importance of type identification of unregistered fishing vessels.

关键词： VMS vessel type identification fishing density trajectory analysis classification

Study on Model of Indoor Air Pollution Forecast for Decoration Under Natural Ventilation Condition

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Biomedical and Environmental Sciences 2005年第5期18卷 345-348页

作者： YAN-feng hong XUN CHEN NING XU Institute for Environmental Health and Related Product Safety Chinese Center for Disease Control and Prevention Beijing 100050 China

To establish the model of indoor air pollution forecast for decoration. Methods The model was based on the balance model for diffusing mass. Results The data between testing concentration and estimating concentration were compared. The maximal error was less than 30% and average error was 14.6%. Conclusion The model can easily predict whether the pollution for decoration exceeds the standard and how long the room is decorated.

关键词： Air pollution indoor Model Natural ventilation

Development of a New High-throughput Screening Model for Human High Density Lipoprotein Receptor (CLA-1) Agonists

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Biomedical and Environmental Sciences 2005年第4期18卷 265-272页

作者： DE-feng TIAN BIN hong SHU-YI SI Institute of Medicinal Biotechnology Chinese Academy of Medical Sciences and Peking Union Medical College Beijing 100050 China

To develop a new high-throughput screening model for human high-density lipoprotein （HDL） receptor （CD36 and LIMPⅡ analogous-1, CLA-1） agonists using CLA-1-expressing insect cells. Methods With the total RNA of human hepatoma cells BEL-7402 as template, the complementary DNA （cDNA） of CLA-1 was amplified by reverse transcription-polymerase chain reaction （RT-PCR）. Bac-to-Bac baculovirus expression system was used to express CLA-1 in insect cells. CLA-1 cDNA was cloned downstream of polyhedrin promoter of Autographa californica nuclear polyhedrosis virus （AcNPV） into donor vector pFastBacl and recombinant pFastBacl-CLA-1 was transformed into E. coli DH10Bac to transpose CLA-1 cDNA to bacrnid DNA. Recombinant bacrnid-CLA-1 was transfected into Spodopterafrugiperda Sf9 insect cells to produce recombinant baculovirus particles. Recombinant CLA- 1 was expressed on the membrane of Sf9 cells infected with the recombinant baculoviruses. A series of parameters of DiI-lipoprotein binding assays of CLA-1-expressing Sf9 cells in 96-well plates were optimized. Results Western blot analysis and DiI-lipoprotein binding assays confirmed that CLA-1 expressed in insect cells had similar immunoreactivity and ligand binding activity as its native counterpart. A reliable and sensitive in vitro cell-based assay was established to assess the activity of CLA-1 and used to screen agonists from different sample libraries. Conclusion Human HDL receptor CLA-1 was successfully expressed in Sf9 insect cells and a novel high-throughput screening model for CLA-1 agonists was developed. Utilization of this model allows us to identify potent and selective CLA-1 agonists which might possibly be used as therapeutics for atherosclerosis.

关键词： HDL SR-BI CLA-1 Insect cells High-throughput screening model Agonist

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On Spinors

Acta Mathematica Sinica,English Series 2009年第10期25卷 1721-1728页

作者： Yan Lin YU Lin ZHU Xiu hong feng Department of Mathematics Suzhou University Suzhou 215006 P. R. China

For a 2^n-dimensional complex Hermitian vector space S, we prove that any unitary basis of S can be explained as an augmented spinor structure on S. By using this explanation, a SpinC（2n）- action on S is equivalent to an action on a subset of augmented spinor structures. The latter action is a little easy to be understood, and is shown in the last part of this paper. Such kind of understanding could be of use to the discussions of Hermitian manifolds and spin manifolds, especially could help to find connections and elliptical operators.

关键词： Pauli matrices spinor augmented spinor

The concept and the accumulation characteristics of unconventional hydrocarbon resources

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Petroleum Science 2015年第4期12卷 563-572页

作者： Yan Song Zhuo Li Lin Jiang feng hong State Key Laboratory of Petroleum Resource and Prospecting China University of Petroleum Unconventional Natural Gas Institute China University of Petroleum Research Institute of Petroleum Exploration and Development Petro China

Unconventional hydrocarbon resources, which are only marginally economically explored and developed by traditional methods and techniques, are different from conventional hydrocarbon resources in their accumulation mechanisms, occurrence states, distribution models, and exploration and development manners. The types of unconventional hydrocarbon are controlled by the evolu- tion of the source rocks and the combinations of different types of unconventional reservoirs. The fundamental dis- tinction between unconventional hydrocarbon resources and conventional hydrocarbon resources is their non- buoyancy-driven migration. The development of the micro- to nano-scale pores results in rather high capillary resis- tance. The accumulation mechanisms of the unconven- tional and the conventional hydrocarbon resources are also greatly different. In conventional hydrocarbon resources, oil and gas entrapment is controlled by reservoir-forming factors and geological events, which is a dynamic balance process; while for unconventional hydrocarbon resources, the gas content is affected by the temperature and pressure fields, and their preservation is crucial. Unconventional and conventional hydrocarbons are distributed in an orderly manner in subsurface space, having three distribution models of intra-source rock, basin-centered, and source rock interlayer. These results will be of great significance to unconventional hydrocarbon exploration.

关键词： Unconventional hydrocarbon resources - Non-buoyancy-driven accumulation. Accumulationmechanisms ~ Distribution model

Effects of repetitive transcranial magnetic stimulation on adenosine triphosphate content and microtubule associated protein-2 expression after cerebral ischemia-reperfusion injury in rat brain

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Chinese Medical Journal 2008年第14期121卷 1307-1312页

作者： feng hong-lin YAN Li CUI Li-ying Department of Neurology First Hospital of Harbin Medical University Harbin Heilongjiang 150001 China Department of Neurology China-Japan Friendship Hospital of Beijing Beijing 100029 China Department of Neurology Peking Union Medical College Hospital Chinese Academy of Medical Sciences and Peking Union Medical College Beijing 100730 China

Background Repetitive transcranial magnetic stimulation （rTMS） research has mainly been focused on the therapeutic effect of psychiatric disorders and Parkinson＇s disease. A few studies have shown that rTMS might protect against delayed neuronal death induced by transient ischemia, enhance long-term potentiation in ischemic conditions and affect regional brain blood flow and metabolism. The aim of this study was to determine the effects of repetitive transcranial magnetic stimulation （rTMS） on adenosine triphosphate （ATP） content and microtubule associated protein-2 （MAP-2） expression in rat brain after middle cerebral artery occlusion （MCAO）/reperfusion. Methods To study the effects of different timecourses of rTMS on ATP content and MAP-2 expression, 90 rats were randomly divided into three groups （30 rats in each group）. To study the effects of multiple rTMS parameters on ATP content and MAP-2 expression, the rats in each group were further divided into six subgroups （five rats each）. The rats were sacrificed at 1-hour, 24-hour and 48-hour intervals after reperfusion, and the brain tissues were collected for the detection of ATP and MAP-2. Results rTMS could significantly increase ATP content and MAP-2 expression in the left brain following ischemic insult （P 〈0.01） and different rTMS parameters had different effects on the ATP level and the MAP-2 expression in the left striatum. A high-frequency rTMS played an important role in MAP-2 expression and ATP preservation. Conclusions This study revealed that rTMS induced significant increase of ATP content and MAP-2 expression in the injured area of the brain, suggesting that the regulation of both ATP and MAP-2 may be involved in the biological mechanism of the effect of rTMS on neural recovery. Therefore, rTMS may become a potential adjunctive therapy for ischemic cerebrovascular disease.

关键词： transcranial magnetic stimulation repetitive cerebral ischemia-reperfusion injury adenosine triphosphate microtubule associated protein-2

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Bispinor Space

Acta Mathematica Sinica,English Series 2007年第9期23卷 1629-1638页

作者： Lin ZHU Xiu hong feng Yan Lin YU Department of Mathematics School of Science Suzhou University Suzhou 215006 P. R. China

In this paper, we give identifications of bispinor space with Grassmann algebra, and with Clifford algebra. The multiplication in Clifford algebra provides an action on them. Lastly we have researched on the geometry ... 详细信息

关键词： spinor bispinor Clifford algebra Jack map