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Chromosome Mapping, Expression and Polymorphism Analysis of CRABP1 Gene in Pigs

Journal of Integrative Agriculture 2014年第5期13卷 1051-1057页

作者： ZHAO Shuan-ping TANG Zhong-lin ZHOU Rong QU chang-qing ZHENG Jian-wei LI Kui Key Laboratory for Domestic Animal Genetic Resources and Breeding Ministry of Agriculture/Institute of Animal Science Chinese Academy of Agricultural Sciences Institute of Animal Science Anhui Academy of Agricultural Sciences School of Life Science Fuyang Teachers College Department of General Surgery Beijing Tiantan Hospital Capital Medical University

Cellular retinoic acid-binding protein 1 （CRABP1） is a well-conserved member of cytosolic lipid-binding protein family. It is an important modulator of retinoic acid signaling. Long serial analysis of gene expression （LongSAGE） analysis suggested that CRABP1 gene was differentially expressed during prenatal skeletal muscle development in porcine. Here, we obtained the full-length coding region sequence and genomic sequence of the porcine CRABP1 gene and analyzed its genomic structures. Subsequently, we examined CRABP1 chromosome assignment using INRA-University of Minnesota 7 000 porcine radiation hybrid panel （IMpRH） and explored its tissue distribution in adult Tongcheng pigs and dynamical expression profiles in prenatal skeletal muscle （33, 65 and 90 days post coitus, dpc） from Landrace （lean-type）（described as L33, L65 and L90） and Tongcheng pigs （obese-type）（described as T33, T65 and T90）. The CRABPI gene was mapped to chromosome 7ql 1-q23 and closely linked to the microsatellite marker SWR1928. Quantitative real-time PCR showed that CRABP1 mRNA was highly expressed in lung and stomach, moderately expressed in placenta and uterus, and weakly expressed in other tissues. Moreover, CRABP1 gene was down-regulated during prenatal skeletal muscle development in both Landrace and Tongcheng pigs and it was expressed much higher in T33 than L33. Two single-nucleotide polymorphisms （SNPs） were detected by sequencing and mass spectrometry methods, allele frequency analysis indicated that g. 281 （G〉A） and g. 2992 （G〉A）were deviated from Hardy-Weinberg equilibrium in the Landrace and DLY （Duroc×（Landrace×Yorkshire）） pig breeds.

关键词： pig CRABP1 chromosome assignment expression profile allele frequency

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房地产税改革的公众情感态度与税收遵从

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税务与经济 2024年第2期 48-58页

作者：许评陈清王伟域北京工商大学经济学院北京100048 国家税务总局税务干部学院江苏扬州225007

利用结构方程模型和新浪微博数据挖掘以及NLP情感分析技术,研究公众对推进我国房地产税改革的情感态度和遵从倾向。研究发现,对税负的感知是引起负面情感态度的最重要因素,且该类情感对税收遵从呈负向显著影响;2021年末,房地产税政策的... 详细信息

利用结构方程模型和新浪微博数据挖掘以及NLP情感分析技术,研究公众对推进我国房地产税改革的情感态度和遵从倾向。研究发现,对税负的感知是引起负面情感态度的最重要因素,且该类情感对税收遵从呈负向显著影响;2021年末,房地产税政策的评价正面情感得分比负面情感略具优势;“试点、房产、房租、房价、贫富、压力、房贷”等是公众最为关注的焦点;负面情感态度的背后存在政策系统性不足、配套支持措施尚未落地、经济压力大等诉求。应同步落实配套支持性政策、实施差异化的房地产税收政策、合理制定税费减免政策、提高税款使用透明度等,以缓解公众对房地产税改革关于税负的负面情感,减轻政策落实的阻力和难度。

关键词：房地产税改革社会情感情感分析税收遵从

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2019~2022年华北平原典型地区不同污染等级和污染持续时间下的O_(3)污染规律及来源

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中国环境科学 2024年第7期44卷 3581-3591页

作者：钱昱先王刚孟庆晓刘赛王永强中国石油大学(华东)化学化工学院山东青岛266580 日照市生态环境局莒县分局山东日照276599

选取地势复杂的日照市莒县为研究区域,基于2019~2022年莒县省控站点的O_(3)监测数据,按照不同O_(3)污染等级与不同O_(3)污染持续时间对莒县的O_(3)污染天进行分类,探究其污染规律,并采用后向轨迹模型(HYSPLIT)、潜在源分析(PSCF)和浓度... 详细信息

选取地势复杂的日照市莒县为研究区域,基于2019~2022年莒县省控站点的O_(3)监测数据,按照不同O_(3)污染等级与不同O_(3)污染持续时间对莒县的O_(3)污染天进行分类,探究其污染规律,并采用后向轨迹模型(HYSPLIT)、潜在源分析(PSCF)和浓度权重轨迹分析法(CWT)识别不同O_(3)污染事件下的O_(3)来源.结果显示:2019~2021年莒县O_(3)轻度污染天数由72d下降到38d,2022年反弹,而中度污染天数变化不大;轻微污染天在轻度污染天中占比较高(21.4%~39.5%),表明轻度污染天的改善空间很大,强化轻微污染天管控措施,将有利于将轻度污染天转化为优良天,提高当地空气质量优良率.O_(3)污染过程持续时间越长(如≥3d),污染发生的频率越低,而O_(3)浓度随污染持续时间的增加而升高.HYSPLIT模拟结果显示不同O_(3)污染等级和不同O_(3)污染持续时间的气团轨迹均以莒县东南方向为主,且以中、短距离传输为主,其中中度污染期间来自东南地区的O_(3)-8h-90%浓度最高[(250.0±65.9)µg/m^(3)].PSCF和CWT结果显示,O_(3)潜在源区主要分布在莒县东南方向,长江三角洲地区是莒县O_(3)污染的主要的潜在源区.研究结果对于指导莒县及周边区域的大气污染联防联控具有重要意义.

关键词：臭氧污染等级污染持续时间后向轨迹潜在源区

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Evaluation of SWAT Model performance on glaciated and non-glaciated subbasins of Nam Co Lake, Southern Tibetan Plateau, China

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Journal of Mountain Science 2019年第5期16卷 1075-1097页

作者： Muhammad ADNAN KANG Shi-chang ZHANG Guo-shuai Muhammad Naveed ANJUM Muhammad ZAMAN ZHANG Yu-qing State Key Laboratory of Cryospheric Science Northwest Institute of Eco-Environment and Resources Chinese Academy of Sciences China University of Chinese Academy of Sciences CAS Centre for Excellence in Tibetan Plateau Earth Sciences Key Laboratory of Tibetan Environment changes and Land Surface Processes Institute of Tibetan Plateau Research Chinese Academy of Sciences Division of Hydrology Water-Land Resources in Cold and Arid Regions Northwest Institute of Eco-Environment and Resources Chinese Academy of Sciences Department of Irrigation & Drainage University of AgricultureFaisalabad Research Center of Fluid Machinery Engineering & Technology Jiangsu University School of Urban and Environmental Sciences Huaiyin Normal University

This paper presents an assessment of the Soil and Water Assessment Tool(SWAT) on a glaciated(Qugaqie) and a non-glaciated(Niyaqu) subbasin of the Nam Co Lake. The Nam Co Lake is located in the southern Tibetan Plateau, two subbasins having catchment areas of 59 km^2 and 388 km^2, respectively. The scores of examined evaluation indices(i.e., R^2, NSE, and PBIAS) established that the performance of the SWAT model was better on the monthly scale compared to the daily scale. The respective monthly values of R^2, NSE, and PBIAS were 0.94, 0.97, and 0.50 for the calibration period while 0.92, 0.88, and -8.80 for the validation period. Glacier melt contribution in the study domain was simulated by using the SWAT model in conjunction with the Degree Day Melt(DDM) approach. The conjunction of DDM with the SWAT Model ensued improved results during both calibration(R^2=0.96, NSE=0.95, and PBIAS=-13.49) and validation (R^2=0.97, NSE=0.96, and PBIAS=-2.87) periods on the monthly time scale. Average contribution(in percentage) of water balance components to the total streamflow of Niyaqu and Qugaqie subbasins was evaluated. We found that the major portion(99.45%) of the streamflow in the Niyaqu subbasin was generated by snowmelt or rainfall surface runoff(SURF_Q), followed by groundwater(GW_Q, 0.47%), and lateral(LAT_Q, 0.06%) flows. Conversely, in the Qugaqie subbasin, major contributor to the streamflow(79.63%) was glacier melt(GLC_Q), followed by SURF_Q(20.14%), GW_Q(0.13%), and LAT_Q(0.089%). The contribution of GLC_Q was the highest(86.79%) in July and lowest(69.95%) in September. This study concludes that the performance of the SWAT model in glaciated catchment is weak without considering glacier component in modeling; however, it performs reasonably well in non-glaciated catchment. Furthermore, the temperature index approach with elevation bands is viable in those catchments where streamflows are driven by snowmelt. Therefore, it is recommended to use the SWAT Model in con

关键词： SWAT Model Nam Co Lake Degree-day melt model Streamflow Hydrological Response Unit

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Tree: Reducing the use of restrictive practices on psychiatric wards through virtual reality immersive technology training

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World Journal of Psychiatry 2024年第10期14卷 1521-1537页

作者： Peter Phiri Laura Pemberton Yang Liu Xiaojie Yang Joe Salmon Isabel Boulter Sana Sajid Jackie Clarke Andy McMillan Jian qing Shi Gayathri Delanerolle Department of Research&Innovation Southern Health National Health Service Foundation TrustSouthampton SO303JBUnited Kingdom Department of Statistics and Data Science Southern University of Science and TechnologyShenzhen 518055Guangdong ProvinceChina School of Statistics and Mathematics Yunnan University of Finance and EconomicsKunming 650221Yunnan ProvinceChina Virti Healthcare KeynshamBristol BS311SNUnited Kingdom Neuromodulation Centre Southern Health National Health Service Foundation TrustSouthampton SO140YGUnited Kingdom National Health Service Talking Therapies Southern Health National Health Service Foundation TrustSouthampton SO509FHUnited Kingdom

BACKGROUND Restrictive practices(RPs)are defined by measures linked to physical and chemical restraints to reduce the movement or control behaviours during any *** is an equal part of RPs intended to isolate and reduce the sensory stimulation to safeguard the patient and those within the *** interventions by way of virtual reality(VR)could assist with reducing the need for RPs as it could help reduce anxiety or agitation by way of placing users into realistic and immersive *** could also aid staff to and change current *** To assess the feasibility and effectiveness of using a VR platform to provide reduction in RP *** A randomised controlled feasibility study,accompanied by evaluations at 1 month and 6 months,was conducted within inpatient psychiatric wards at Southern Health National Health Service Foundation Trust,United *** VR scenarios were used on VR headsets to provide training on reducing RPs in 3 inpatient psychiatric *** measures included general self-efficacy scale,generalised anxiety disorder assessment 7(GAD-7),Burnout Assessment Tool 12,the Everyday Discrimination Scale,and the Compassionate Engagement and Action *** Findings revealed statistically significant differences between the VR and treatment as usual groups,in the Everyday Discrimination Scale items Q8 and Q9:P=0.023 and P=0.040 respectively,indicating higher levels of perceived discrimination in the VR *** were no significant differences between groups in terms of general self-efficacy,generalised anxiety disorder assessment 9,and Burnout Assessment Tool 12 scores.A significant difference was observed within the VR group for compassionate engagement from others(P=0.005)over *** respondents recorded System Usability Scale scores above 70,with an average score of *** was a significant reduction in rates of RPs in the VR group vs treatment as usual group with a fluctuating variability ob

关键词： Virtual reality Restrictive practices Inpatient wards Restraint Isolation Rapid tranquilisation Covert medication Procedural restrictions Health professions training

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Molecular mechanisms and the role of the protein kinase pathway in rat spatial memory impairment following isoflurane anesthesia

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Neural Regeneration Research 2010年第7期5卷 508-513页

作者： qing Zhang Guanyu Lu Zhipeng Xu Chunsheng feng Anshi Wu Yun Wang Hui Wang Yun Yue Department of Anesthesia Beijing Chaoyang Hospital Capital Medical University Beijing 100020 China

BACKGROUND： Animal experiments have demonstrated that isoflurane exposure alone induces learning and memory deficits for weeks or months. However, the molecular mechanisms of learning and memory remain poorly understood. Hippocampal expression of calcium/phospholipid- dependent protein kinase （PKC） and cAMP-dependent protein kinase （PKA） in rats have been shown to be associated with memory processing. OBJECTIVE： To investigate changes in rat spatial memory and hippocampal CA1 neuronal kinase system following isoflurane anesthesia, and to explore the correlation between molecular changes in cerebral neurons and behavioral manifestations following anesthesia. DESIGN, TIME AND SE＇rFING： A randomized, controlled, animal study. All experiments were performed at the Department of Anesthesia, Beijing Chaoyang Hospital, Capital Medical University from November 2007 to December 2008. MATERIALS： A total of 72 male, 3 month-old （young group）, Sprague Dawley rats, and 36 male, 20 month-old （aged group）, Sprague-Dawley rats were used in the study. Isoflurane was purchased from Baxter, USA. METHODS： Young and aged rats were randomly assigned to control, training （no anesthesia, Morris water maze training）, and isoflurane （1.2% isoflurane, Morris water maze training） groups. The isoflurane group was further subdivided into four groups, which were exposed to anesthesia for 2 or 4 hours, and were subjected to Morris water maze training at 2 days or 2 weeks postanesthesia. Finally, each aged group comprised 6 rats, and the young group comprised 12 rats. MAIN OUTCOME MEASURES： Spatial learning and memory were observed during Morris water maze training. Hippocampal CA1 PKA and PKC expression and activity were detected by immunohistochemistry and enzyme-linked immunosorbent assay （ELISA）. RESULTS： A 4-hour isoflurane exposure induced spatial memory deficits in all rats for 2 days to 2 weeks. In particular, aged rats exhibited more severe spatial memory deficits. Immunohistochemistry and

关键词： isoflurane Morris water maze hippocampus cAMP-dependent protein kinase calcium/phospholipid-dependent protein kinase

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整体型认知框架、商业模式创新与新创企业绩效——基于环境动态性调节作用的视角

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税务与经济 2024年第4期 91-98页

作者：王超张树山李清东北师范大学经济与管理学院吉林长春130117 长春大学管理学院吉林长春130022

整体型认知框架是加强创业者关注环境中信息关联性的重要认知因素,且该类认知对商业模式创新和新创企业绩效均具有正向显著影响;商业模式创新在整体型认知框架与新创企业绩效的关系间起中介作用,整体型认知帮助创业者关注不同商业元素... 详细信息

整体型认知框架是加强创业者关注环境中信息关联性的重要认知因素,且该类认知对商业模式创新和新创企业绩效均具有正向显著影响;商业模式创新在整体型认知框架与新创企业绩效的关系间起中介作用,整体型认知帮助创业者关注不同商业元素之间的隐藏关联,有助于创新商业模式,进而提高新创企业绩效;此外,环境动态性给新企业创新与成长带来了更多的机会与挑战,其正向调节整体型认知框架与商业模式创新的关系。创业者需要提高自身与团队的认知能力,关注事物的整体性和关联性,打破认知定势,提高管理团队决策认知的全面性和灵活性,同时要建立应对环境动态性的治理机制,继而促进企业商业模式创新,助力新创企业绩效提升。

关键词：整体型认知框架新创企业绩效商业模式创新环境动态性

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Fault diagnosis and process monitoring using a statistical pattern framework based on a self-organizing map

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Journal of Central South University 2015年第2期22卷 601-609页

作者：宋羽姜庆超颜学峰 Key Laboratory of Advanced Control and Optimization for Chemical Processes of Ministry of Education(East China University of Science and Technology)

A multivariate method for fault diagnosis and process monitoring is proposed. This technique is based on a statistical pattern(SP) framework integrated with a self-organizing map(SOM). An SP-based SOM is used as a classifier to distinguish various states on the output map, which can visually monitor abnormal states. A case study of the Tennessee Eastman(TE) process is presented to demonstrate the fault diagnosis and process monitoring performance of the proposed method. Results show that the SP-based SOM method is a visual tool for real-time monitoring and fault diagnosis that can be used in complex chemical *** with other SOM-based methods, the proposed method can more efficiently monitor and diagnose faults.

关键词： statistic pattern framework self-organizing map fault diagnosis process monitoring

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干旱胁迫下沙棘CMO基因的时空表达模式与蛋白结构预测

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西北林学院学报 2024年第2期39卷 21-27页

作者：刘青青马玉花董佳伟冶贵生张丹杨开宇青海大学农牧学院青海西宁810016

CMO基因是沙棘体内甜菜碱合成过程中的关键性基因,对植物体抗旱起着重要作用。以青海野生中国沙棘的根、茎、叶为试验材料,对沙棘CMO基因及其全长序列进行克隆,并进行生物信息学分析,在此基础上对不同程度干旱胁迫下沙棘不同组织部位CM... 详细信息

CMO基因是沙棘体内甜菜碱合成过程中的关键性基因,对植物体抗旱起着重要作用。以青海野生中国沙棘的根、茎、叶为试验材料,对沙棘CMO基因及其全长序列进行克隆,并进行生物信息学分析,在此基础上对不同程度干旱胁迫下沙棘不同组织部位CMO基因的时空表达模式进行研究。结果表明,沙棘CMO基因全长1566 bp,ORF长1364 bp,编码454个氨基酸,分子量为51.41 ku,等电点为6.78;CMO蛋白亚细胞定位于叶绿体,无信号肽,蛋白二级结构主要为无规则卷曲;CMO蛋白具有多个多种类型的功能位点,为蛋白功能的实现提供了保障;此外CMO蛋白的亲水性较强,无跨膜螺旋区;CMO蛋白三维结构由α螺旋、β折叠和无规则卷曲互相盘绕而成;时空表达模式研究表明沙棘CMO基因的表达明显受到干旱胁迫的影响,CMO基因的表达量随胁迫时间的延长而整体呈逐步升高的趋势,直至胁迫末期或复水后才有所降低;CMO基因在沙棘不同组织部位中的表达有一定的差异,表达量大小为根>叶>茎。通过对沙棘CMO基因的研究分析,以期为提高沙棘抗旱性及植物CMO基因的深入研究提供参考意义。

关键词：沙棘干旱胁迫 CMO基因蛋白质结构

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Overexpression of IbSnRK1 enhances nitrogen uptake and carbon assimilation in transgenic sweetpotato

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Journal of Integrative Agriculture 2018年第2期17卷 296-305页

作者： REN Zhi-tong ZHAO Hong-yuan HE Shao-zhen ZHAI Hong ZHAO Ning LIU qing-chang Key Laboratory of Sweetpotato Biology and Biotechnology Ministry of Agriculture/Beijing Key Laboratory of Crop Genetic Improvement/Laboratory of Crop Heterosis and Utilization Ministry of Education/China Agricultural University

Nitrogen is an important nutrient for plant development. Nitrogen and carbon metabolisms are tightly linked to physiological functions in plants. In this study, we found that the IbSnRK1 gene was induced by Ca（NO3）2. Its overexpression enhanced nitrogen uptake and carbon assimilation in transgenic sweetpotato. After Ca（^15NO3）2 treatment, the -（15）N atom excess, -（15）N and total N content and nitrogen uptake efficiency（NUE） were significantly increased in the roots, stems, and leaves of transgenic plants compared with wild type（WT） and empty vector control（VC）. After Ca（NO3）2 treatment, the increased nitrate N content, nitrate reductase（NR） activity, free amino acid content, and soluble protein content were found in the roots or leaves of transgenic plants. The photosynthesis and carbon assimilation were enhanced. These results suggest that the IbSnRK1 gene play a important role in nitrogen uptake and carbon assimilation of sweetpotato. This gene has the potential to be used for improving the yield and quality of sweetpotato.

关键词： carbon assimilation IbSnRK1 nitrogen uptake sweetpotato

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