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Transcriptome and Regulatory Network Analyses of CD19-CAR-T Immunotherapy for B-ALL

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Genomics, Proteomics & Bioinformatics 2019年第2期17卷 190-200页

作者： Qiong Zhang hui hu Si-Yi Chen Chun-Jie Liu fei-fei hu Jianming Yu Yaohui Wu An-Yuan Guo hubei Bioinformatics and Molecular Imaging Key Laboratory Department of Bioinformatics and Systems Biology Key Laboratory of Molecular Biophysics of the Ministry of Education College of Life Science and Technology Huazhong University of Science and Technology Wuhan 430074 China Institute of Hematology Union Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan 430022 China

Chimeric antigen receptor (CAR) T cell therapy has exhibited dramatic anti-tumor effi-cacy in clinical trials. In this study,we reported the transcriptome profiles of bone marrow cells in four B cell acute lymphoblastic leukemia (B-ALL) patients before and after CD19-specific CAR-T therapy. CD19-CAR-T therapy remarkably reduced the number of leukemia cells,and three patients achieved bone marrow remission (minimal residual disease negative). The efficacy of CD19-CAR-T therapy on B-ALL was positively correlated with the abundance of CAR and immune cell subpopulations,e.g.,CD8+T cells and natural killer (NK) cells,in the bone marrow. Additionally,CD19-CAR-T therapy mainly influenced the expression of genes linked to cell cycle and immune response pathways,including the NK cell mediated cytotoxicity and NOD-like recep-tor signaling pathways. The regulatory network analyses revealed that microRNAs (e.g.,miR-148a-3p and miR-375),acting as oncogenes or tumor suppressors,could regulate the crosstalk between the genes encoding transcription factors (TFs,e.g.,JUN and FOS) and histones (e.g.,HIST1H4A and HIST2H4A) involved in CD19-CAR-T therapy. Furthermore,many long non-coding RNAs showed a high degree of co-expression with TFs or histones (e.g.,FOS and HIST1H4B) and were associated with immune processes. These transcriptome analyses provided important clues for fur-ther understanding the gene expression and related mechanisms underlying the efficacy of CAR-T immunotherapy.

关键词： CAR-T B-ALL Transcriptome profile lncRNA Regulatory network

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微藻中棕榈油酸的研究进展

天然产物研究与开发 2017年第7期29卷 1240-1247,1198页

作者：王飞飞胡强张成武暨南大学水生生物研究中心广州510632 中国科学院水生生物研究所武汉430072

棕榈油酸是一种ω-7单不饱和脂肪酸,它具有重要的营养和医药保健价值,同时也是制备生物柴油和1-辛烯的优质原料。棕榈油酸普遍存在油料作物中,但是含量较低,而含量较高的野生植物又存在资源少问题,因此利用富含棕榈油酸且适合大规模培... 详细信息

棕榈油酸是一种ω-7单不饱和脂肪酸,它具有重要的营养和医药保健价值,同时也是制备生物柴油和1-辛烯的优质原料。棕榈油酸普遍存在油料作物中,但是含量较低,而含量较高的野生植物又存在资源少问题,因此利用富含棕榈油酸且适合大规模培养的微藻是解决棕榈油酸来源的一条新型途径。本文重点介绍了微藻中棕榈油酸来源的优势、棕榈油酸的生理功能、微藻中棕榈油酸的合成途径及分离纯化方法,并且展望了棕榈油酸进一步的研究方向。

关键词：微藻棕榈油酸生理功能合成途径分离纯化

miRNA-21-5p is an important contributor to the promotion of injured peripheral nerve regeneration using hypoxia-pretreated bone marrow-derived neural crest cells

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Neural Regeneration Research 2025年第1期20卷 277-290页

作者： Meng Cong Jing-Jing hu Yan Yu Xiao-Li Li Xiao-Ting Sun Li-Ting Wang Xia Wu Ling-Jie Zhu Xiao-Jia Yang Qian-Ru He fei Ding Hai-Yan Shi School of Medicine Nantong UniversityNantongJiangsu ProvinceChina Key Laboratory of Neuroregeneration of Jiangsu and Ministry of Education and Coinnovation Center of Neuroregeneration Nantong UniversityNantongJiangsu ProvinceChina Department of Physiology Jiangsu Health Vocational CollegeNanjingJiangsu ProvinceChina Department of Pathology Affiliated Hospital of Nantong UniversityNantongJiangsu ProvinceChina Jiangsu Clinical Medicine Center of Tissue Engineering and Nerve Injury Repair Affiliated Hospital of Nantong UniversityNantongJiangsu ProvinceChina

Our previous study found that rat bone marrow–derived neural crest cells(acting as Schwann cell progenitors)have the potential to promote long-distance nerve ***-based therapy can enhance peripheral nerve repair and regeneration through paracrine bioactive factors and intercellular ***,the complex contributions of various types of soluble cytokines and extracellular vesicle cargos to the secretome remain *** investigate the role of the secretome and extracellular vesicles in repairing damaged peripheral nerves,we collected conditioned culture medium from hypoxia-pretreated neural crest cells,and found that it significantly promoted the repair of sensory neurons damaged by oxygen-glucose *** mRNA expression of trophic factors was highly expressed in hypoxia-pretreated neural crest *** performed RNA sequencing and bioinformatics analysis and found that miR-21-5p was enriched in hypoxia-pretreated extracellular vesicles of neural crest ***,to further clarify the role of hypoxia-pretreated neural crest cell extracellular vesicles rich in miR-21-5p in axonal growth and regeneration of sensory neurons,we used a microfluidic axonal dissociation model of sensory neurons in vitro,and found that hypoxia-pretreated neural crest cell extracellular vesicles promoted axonal growth and regeneration of sensory neurons,which was greatly dependent on loaded ***,we constructed a miR-21-5p-loaded neural conduit to repair the sciatic nerve defect in rats and found that the motor and sensory functions of injured rat hind limb,as well as muscle tissue morphology of the hind limbs,were obviously *** findings suggest that hypoxia-pretreated neural crest extracellular vesicles are natural nanoparticles rich in ***-21-5p is one of the main contributors to promoting nerve regeneration by the neural crest cell *** helps to explain the mechanism of action of the secretome and e

关键词： axotomy cell-free therapy conditioned medium extracellular vesicles hypoxic preconditioning microRNA oxygen-glucose deprivation peripheral nerve injury Schwann cell precursors

“互联网+”背景下航空公司混合营销渠道发展研究

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广义虚拟经济研究 2017年第2期8卷 36-40页

作者：张菲菲胡荣陈琳南京航空航天大学民航学院江苏南京210016

近年来,"互联网+"因其跨界融合、创新驱动、重塑结构、尊重人性、开放生态等特点,适应了当今社会的发展潮流,成为各个领域热议的话题。本文围绕"互联网+"发展理念与趋势,研究航空公司混合营销渠道创新发展策略,深入分析了"互联网+"的概... 详细信息

近年来,"互联网+"因其跨界融合、创新驱动、重塑结构、尊重人性、开放生态等特点,适应了当今社会的发展潮流,成为各个领域热议的话题。本文围绕"互联网+"发展理念与趋势,研究航空公司混合营销渠道创新发展策略,深入分析了"互联网+"的概念以及特征,阐述了"互联网+"背景下国内外航空公司混合营销渠道发展的特点,结合"互联网+"的新优势提出了关系协调和整合优化策略,以期创新发展航空公司混合营销渠道的研究,进一步提高航空公司的综合竞争力。

关键词：航空运输混合营销渠道 “互联网+”

土体抗剪强度各向异性对边坡滑动模式与稳定性的影响

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江苏大学学报（自然科学版） 2025年第1期46卷 91-97页

作者：张飞胡光华河海大学岩土力学与堤坝工程教育部重点实验室江苏南京210024 河海大学江苏省岩土工程技术工程研究中心江苏南京210024

基于极限分析法中的上限定理,开展了考虑土体抗剪强度各向异性的边坡稳定性研究,通过参数分析研究了土体抗剪强度各向异性对边坡失稳滑动方式与边坡稳定性安全系数的影响规律.结果表明:边坡稳定性安全系数随着抗剪强度各向异性系数减小... 详细信息

基于极限分析法中的上限定理,开展了考虑土体抗剪强度各向异性的边坡稳定性研究,通过参数分析研究了土体抗剪强度各向异性对边坡失稳滑动方式与边坡稳定性安全系数的影响规律.结果表明:边坡稳定性安全系数随着抗剪强度各向异性系数减小而减小,其影响程度随着土体内摩擦角和坡度的减小而增大;对于土体黏聚力较大、内摩擦角较小的缓坡,随着抗剪强度各向异性系数减小,边坡的最危险滑动面会由坡脚深层破坏转变为坡底深层破坏,滑动范围显著增大;基于计算结果绘制了稳定图(stability chart),实现了考虑土体抗剪强度各向异性影响的边坡稳定性安全系数的快速计算与最危险滑动方式的判别.

关键词：边坡抗剪强度各向异性稳定性安全系数极限分析法滑动

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汇交地质资料电子文件原始性保证技术研究

中国矿业 2016年第11期25卷 72-75页

作者：马飞飞胡麟臻王成锡郭慧锦中国地质大学(武汉)国家地理信息系统工程技术研究中心湖北武汉430074 中国地质调查局发展研究中心北京100037 全国地质资料馆北京100037

地质资料的原始性是地质资料真实、可靠的前提,是地质资料汇交过程中急需解决的难题。本文基于目前我国保证地质资料原始性工作的发展历史与现状,利用可行时间戳、数字签名、MD5散列算法三种不同原始性保证技术,并对比这三种方法在汇交... 详细信息

地质资料的原始性是地质资料真实、可靠的前提,是地质资料汇交过程中急需解决的难题。本文基于目前我国保证地质资料原始性工作的发展历史与现状,利用可行时间戳、数字签名、MD5散列算法三种不同原始性保证技术,并对比这三种方法在汇交地质资料方面的优缺点。通过实践证明MD5散列算法在地质资料原始性检验方面具有操作简单、成本低、无负担等优势,但是由于地质资料保密性和数据格式复杂等原因,MD5散列算法尚需完善。

关键词：地质资料原始性 MD5散列算法

优秀男子跳远运动员起跳过程中起跳腿肌肉用力特征研究

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体育研究与教育 2017年第5期32卷 105-108页

作者：罗菲菲胡国宁刘生杰山西大学体育学院山西太原030006

为揭示起跳腿肌肉在起跳阶段的用力特点及其与跳远成绩的关系,运用Vicon动作捕捉系统、三维测力台和表面肌电仪三机同步综合测试的方法,对优秀跳远运动员起跳阶段的肌群用力特点进行全面综合地分析。结果表明:跳远运动员在起跳阶段的技... 详细信息

为揭示起跳腿肌肉在起跳阶段的用力特点及其与跳远成绩的关系,运用Vicon动作捕捉系统、三维测力台和表面肌电仪三机同步综合测试的方法,对优秀跳远运动员起跳阶段的肌群用力特点进行全面综合地分析。结果表明:跳远运动员在起跳阶段的技术运用、肌力特点以及皮肤表面肌电特征表现为:当起跳腿处于最大缓冲时,垂直反作用力值最大;水平反作用力是运动员获得冲量的主要动力;踝关节力量的大小影响着垂直与水平方向的分力;在起跳阶段的不同时间肌肉的收缩次序、发力程度和持续过程各不相同。

关键词：跳远运动员起跳腿肌肉用力

On a Spanning K-tree Containing Specified Vertices in a Graph

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Acta Mathematicae Applicatae Sinica 2019年第4期35卷 919-923页

作者： fei-fei SONG Zhi-quan hu Department of Information & Computational Science He’nan Agricultural University Zhengzhou 450002 China Faculty ofMath & Statistics Central China Normal UniversityWuhan 430079 China

A k-tree is a tree with maximum degree at most k. In this paper, we give a sharp degree sum condition for a graph to have a spanning k-tree in which specified vertices have degree less than t, where 1≤t≤*** denote by σ_k(G) the minimum value of the degree sum of k independent vertices in a graph G. Let k≥2,s≥0 and 1≤t≤k be integers, and suppose G is an(s + 1)-connected graph with σ_k(G)≥|G|+(k-t)*** for any s specified vertices, G contains a spanning k-tree in which every specified vertex has degree at most t. This improves a result obtained by Matsuda and Matsumura.

关键词： spanning tree k-tree (s + 1)-connected graphs degree sum condition specified vertices

A wide-linear-range and low-hysteresis resistive strain sensor made of double-threaded conductive yarn for human movement detection

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Journal of Materials Science & Technology 2024年第5期172卷 202-212页

作者： fei huang Jiyong hu Xiong Yan Shanghai Frontier Science Research Center for Modern Textiles Donghua UniversityShanghai 201620China

Yarn-based flexible strain sensors with advantages in wearability and integrability have attracted wide ***,it is still a big challenge to achieve yarn-based strain sensors with a wide linear strain range,low hysteresis,and durability synchronously that can be used for full range detection of human body ***,a new structure,double-threaded conductive yarn with rhythmic strain distribu-tion,is reported to markedly widen the linear strain range of microcrack-based stretchable strain sensors.A new method of winding and thermally adhering hot-melt filaments on the surface of the elastic fiber is used to achieve double-threaded yarn(DTY)with rhythmic strain *** proposed strategy,the integration of heterogeneous materials,is reported to significantly reduce the mechanical hysteresis of composite *** strain distribution of the DTY during stretching causes multi-level micro-cracks in different regions of the carbon nanotube(CNT)conductive layer deposited on the surface of the ***,the sensing performance of DTY-based strain sensor can be adjusted by designing the structural *** final prepared flexible strain sensor has the advantages of a wide linear strain range(100%),great sensitivity(GF=12.43),low hysteresis,rapid response(158 ms),high repeatability(>2000 cycles at 50%strain),and hydrophobicity,*** sensor can monitor human motion repeatedly and stably well,and shows great advantages in flexible wearable devices.

关键词： Double-threaded yarn Strain redistribution Hysteresis human motion monitoring