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Direct Shoot Regeneration from Callus of Melicope lunu-ankenda

Natural Science 2015年第2期7卷 81-87页

作者： Ab. Zuraida Rahman Ayu Nazreena Othman fatin liyana izzati kamaruddin Aziz Bin Ahmad Biotechnology Research Centre MARDI Headquarters Persiaran MARDI-UPM Serdang Malaysia School of Science and Food Technology Universiti Malaysia Terengganu Kuala Nerus Malaysia Centre for Fundamental and Liberal Education Universiti Malaysia Terengganu Kuala Nerus Malaysia

Melicope lunu-ankenda is commonly used in traditional medicine. The conventional propagation method for this species is inefficient due to low propagation rate and its lengthy period to maturity. In addition, insufficient planting materials often pose a problem for the plantation sector. The tissue culture technique is best alternative to overcome the problems. The callus induction and direct shoot regeneration protocols for M. lunu-ankenda were established. Callus was successfully initiated from leaves explants cultured in MS medium added with 2,4-D at concentrations 0.5 to 5.0 mg/L singly or in combination with NAA at concentrations 1.0 to 10 mg/L. Shoot was regenerated from callus in phytohormone-free medium, BAP at concentrations 0.5 - 5.0 mg/L singly or in combination of BAP with NAA or 2,4-D at concentration 0.5 and 1.0 mg/L, respectively. BAP at 1.0 mg/L induced the highest shoot regeneration rate (80%) and number of plantlet per calli. The established methods might be used for production of phytochemicals and plantlets in large scale.

关键词： Melicope lunu-ankenda Regeneration Plant Growth Regulator In Vitro

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Establishment and Optimization Growth of Shoot Buds-Derived Callus and Suspension Cell Cultures of Kaempferia parviflora

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American Journal of Plant Sciences 2014年第18期5卷 2693-2699页

作者： Ab Rahman Zuraida Othman Ayu Nazreena Kamarulzaman fatin liyana izzati Ahmad Aziz Biotechnology Research Centre MARDI Headquarters MARDI HQ Persiaran MARDI-UPM Serdang Malaysia School of Science and Food Technology Kuala Terengganu Malaysia

Callus and suspension cells culture of Kaempferia parviflora was successfully established. Meristematic shoots can be used for utilization of plant cell biosynthetic capabilities for obtaining useful products from valuable medicinal plant to meet out the pharmaceutical demand and also for studying the metabolism. The medium containing combination of 0.2 mg/L 2,4-dichlorophenoxyacetic acid (2,4-D) and 0.2 mg/L napthyleneacetic acid (NAA) promoted the highest callus induction at 20%. Transferring the initiated callus on the medium with 1 mg/L 2,4-D enhanced the proliferation rate up to maximum fresh weight of 6.71 gm. Growth curve of cultured cells revealed that the cells continued to grow until 50 days of culture and showed the highest peak (fresh weight) at 40 days in all different initial weight tested ( 0.2, 0.5 and 1.0 gram). Isolated embryogenic callus was found to produce the highest in weight when suspended in liquid medium supplemented with 1 mg/L 2,4-D at 110 rpm resulted 13.5 gram fresh weight and 1080 mg dry weight.

关键词： Kaempferia parviflora Suspension Cultures Meristematic Shoots Callus Induction

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In VitroRegeneration of Curcuma caesiaPlantlets from Basal Part and via Somatic Embryogenesis

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Advances in Bioscience and Biotechnology 2014年第4期5卷 363-372页

作者： Ab. Rahman Zuraida kamaruddin fatin liyana izzati Othman Ayu Nazreena Che Mohd Zain Che Radziah Sheikh Ghadzi Siti Nur Asyikin Subramaniam Sreeramanan Biotechnology Research Centre MARDI Headquarters Persiaran MARDI-UPM Serdang Malaysia Faculty of Science and Technology Universiti Kebang-saan Malaysia Bangi Malaysia School of Biological Sciences Universiti Sains Malaysia (USM) Minden Heights Malaysia

Plantlets of Curcuma caesia were produced in vitro from newly sprouting vegetative buds of tubers. Segments of the plantlets from the junction between the root and the basal portion of the stem were subsequently used as explants to investigate factors affecting callus induction and plant regeneration via somatic embryogenesis. The explants were placed on Woody Plant Medium (WPM) together with different concentrations of 2,4-dichlorophenoxyacetic acid(2,4-D) and benzyl aminopurine (BAP) in the presence of light. The growth medium supplemented with 5 mg/L BAP and 2 mg/L 2,4-D promoted callus induction after 70 days of culture. Sub-culturing on the same medium enhanced the production of friable callus. Culture media containing higher concentrations of agar promoted the development of green somatic embryos from the callus. Respond of somatic embryogenesis was most successful with MS medium in 6.0 g/L agar supplemented with 5 mg/L BAP and 0.2 mg/L 2,4-D whereby the callus developed into green somatic embryos with an efficiency of 53%. This culture medium also produced the largest number plantlets.

关键词： In Vitro Regeneration Somatic Embryogenesis Curcuma caesia

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A Simple and Efficient Protocol for the Mass Propagation of Vanilla planifolia

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American Journal of Plant Sciences 2013年第9期4卷 1685-1692页

作者： Ab Rahman Zuraida Kamarulzaman Hassan fatin liyana izzati Othman Ayu Nazreena Wan Sembok Wan Zaliha Che Mohd Zain Che Radziah Zainal Zamri Subramaniam Sreeramanan Biotechnology Research Centre MARDI Headquarters MARDI HQ Persiaran MARDI-UPM Serdang Selangor Malaysia Department of Agrotechnology Faculty of Agrotechnology and Food Science Universiti Malaysia Terengganu Kuala Terengganu Malaysia Faculty of Science and Technology Universiti Kebangsaan Malaysia Bangi Malaysia School of Biological Sciences Universiti Sains Malaysia (USM) Minden Heights Malaysia.

The present study describes a direct shoot regeneration-based micropropagation procedure for Vanilla planifolia. Two types of explant (i.e. shoot apex and stem nodal segment) were screened for their shoot induction potential following a three-month treatment with 6-benzylaminopurine (BAP) and α-Naphthaleneacetic acid (NAA). Results indicated that the shoot apices were poor candidates for shoot induction whereas the stem nodal segments showed potential for shoot initiation at a rate of up to 6 shoots/explant. Stem nodal segments were the most responsive as shoots formed (55 shoots) directly following treatments with 1 mg/L BAP at half strength MS medium after the third subculture. In addition, more shoots were produced on solid medium treatments compared to the liquid medium treatments in two strengths of mediums tested. Regenerated plantlets derived from the 1 mg/L of BAP treatment were induced to root following a one month culture in growth regulator-free MS medium. There was 90% survival rate of the rooted plantlets after acclimatization in the greenhouse. The findings in the present study would be helpful for large-scale mass propagation of Vanilla planifolia using this simple and efficient protocol.

关键词： Vanilla planifolia Mass Propagation Growth Regulator In Vitro BAP NAA IBA

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