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Molecular Cloning and Characterization of an Allene Oxide Cyclase Gene Associated with Fiber Strength in Cotton

Journal of Integrative Agriculture 2014年第10期13卷 2113-2121页

作者： WANG Li-man ZHU You-min TONG Xiang-chao HU Wen-jing CAI Cai-ping guo Wang-zhen State Key Laboratory of Crop Genetics & Germplasm Enhancement Hybrid Cotton R&D Engineering Research CenterMinistry of Education/College of AgricultureNanjing Agricultural University

Allene oxide cyclase （AOC） is one of the most important enzymes in the biosynthetic pathway of the plant hormone jasmonic acid （JA）. AOC catalyzes the conversion ofallene oxide into 12-oxo-phytodienoic acid （OPDA）, a precursor of JA. Using 28K cotton genome array hybridization, an expressed sequence tag （EST; GenBank accession no. ES792958） was investigated that exhibited significant expression differences between lintless-fuzzless XinWX and linted-fuzzless XinFLM isogenic lines during fiber initiation stages. The EST was used to search the Gossypium EST database （http：//***/） for corresponding cDNA sequences encoding full-length open reading frames （ORFs）. Identified ORFs were confirmed using transcriptional and genomic data. As a result, a novel gene encoding AOC in cotton （Gossypium hirsutum AOC; GenBank accession no. KF383427） was cloned and characterized. The 741-bp GhAOC gene comprises three exons and two introns and encodes a polypeptide of 246 amino acids. Two homologous copies were identified in the tetraploid cotton species G. hirsutum acc. TM-1 and G. barbadense cv. Hai7124, and one copy in the diploid cotton species G. herbaceum and G. raimondii, qRT-PCR showed that the GhAOC transcript was abundant in cotton fiber tissues from 8 to 23 days post anthesis （DPA）, and the expression profiles were similar in the two cultivated tetraploid cotton species G. hirsutum acc. TM- 1 and G. barbadense cv. Hai7124, with a higher level of transcription in the former. One copy of GhAOC in tetraploid cotton was localized to chromosome 24 （Chr. D8） using the subgenome-specific single nucleotide polymorphism （SNP） marker analysis, which co-localized GhAOC to within 10 cM of a fiber strength quantitative trait locus （QTL） reported previously. GhAOC was highly correlated with fiber quality and strength （P=0.014） in an association analysis, suggesting a possible role in cotton fiber development, especially in secondary cell wall thickening.

关键词： allene oxide cyclase structure expression pattern fiber development association analysis cotton

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智能静脉用药调配机器人在日间化疗药物调配中的应用

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海南医学 2024年第15期35卷 2214-2217页

作者：刘芳潘颖深圳市第二人民医院药学部广东深圳518035

目的探讨智能静脉用药调配机器人在日间化疗药物调配中的应用效果。方法深圳市第二人民医院2023年1~12月共调配15870袋化疗药物,将其中2023年1~6月人工药物调配的9566袋作为对照组,2023年7~12月智能静脉用药调配机器人调配的6304袋作为... 详细信息

目的探讨智能静脉用药调配机器人在日间化疗药物调配中的应用效果。方法深圳市第二人民医院2023年1~12月共调配15870袋化疗药物,将其中2023年1~6月人工药物调配的9566袋作为对照组,2023年7~12月智能静脉用药调配机器人调配的6304袋作为观察组。比较两组药物调配效果、药物调配不良事件发生率和调配人员健康损伤事件发生率。结果观察组药物的审方时间为(3.37±0.69)min,明显短于对照组的(6.82±1.27)min,配药时间为(1.69±0.55)min,明显长于对照组的(1.54±0.51)min,差异均有统计学意义(P<0.05);每组随机抽查的200瓶化疗药物中,观察组化疗药液残留率为4.00%,明显低于对照组的10.00%,差异有统计学意义(P<0.05);观察组药物调配不良事件总发生率为0.02%,明显低于对照组的0.10%,差异有统计学意义(P<0.05);对照组在操作期间发生10起调配人员刺伤事件,观察组无健康损伤事件发生。结论智能静脉用药调配机器人在日间化疗药物调配中的应用效果明显,可缩短审方时间、配药时间,提高工作效率、减少调配人员健康损伤事件发生率,值得临床推广。

关键词：化疗静脉用药调配中心智能静脉用药调配机器人效果不良事件

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Highly dispersed 1 nm Pt Pd bimetallic clusters for formic acid electrooxidation through a CO-free mechanism

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Journal of Energy Chemistry 2023年第3期78卷 554-564,I0015页

作者： Zhongying fang Ziwei Zhang Shemsu Ligani Fereja Jinhan guo Xinjie Tong Yue Zheng Rupeng Liu Xiaolong Liang Leting Zhang Zongjun Li Wei Chen State Key Laboratory of Electroanalytical Chemistry Changchun Institute of Applied ChemistryChinese Academy of SciencesChangchun 130022JilinChina School of Applied Chemistry and Engineering University of Science and Technology of ChinaHefei 230026AnhuiChina School of Material Science and Technology Jilin Institute of Chemical TechnologyJilin 132022JilinChina School of Chemistry and Pharmaceutical Sciences Guangxi Normal UniversityGuilin 541004GuangxiChina

Direct formic acid fuel cell(DFAFC) is an important research project in clean energy ***,commercialization of DFAFC is still largely limited by the available catalysts with unsatisfied activity,durability and cost for formic acid electrooxidation(FAEO).Using Pt-and Pd-based nanoclusters as electrocatalysts is a particularly promising strategy to solve the above problem,but two attendant problems need to be solved firstly.(Ⅰ) The controllable synthesis of practicable and stable sub-2 nm clusters remains challenging.(Ⅱ) The catalyzing mechanism of sub-2 nm metal clusters for FAEO has not yet completely ***,different from traditional solution synthesis,by designing a novel supporting material containing electron-rich and electron-deficient functional groups,size-and dispersioncontrollable synthesis of ~1 nm PtPd nanoclusters is realized by an electrochemical *** electrocatalytic properties and reaction mechanism of the PtPd nanoclusters for the FAEO were studied by different electrochemical techniques,in-situ fourier transform infrared(FTIR) spectra and density functional theory(DFT) *** tiny PtPd nanoclusters have much higher catalytic activity and durability than commercial Pt/C,Pd/C and 3.5 nm PtPd *** present study shows that the metalreactant interaction plays a decisive role in determining the catalytic activity and cluster-support interaction plays a decisive role in enhancing the durability of *** ratio and arrangement of Pt and Pd atoms on the surface of 1 nm PtPd cluster as well as the overall valence state,d-band center and specific surface area make them exhibit different catalytic performance and reaction mechanism from nanoparticle *** addition,in situ FTIR and DFT calculations showed that on the surface of PtPd clusters,the generation of CO_(2)through trans-COOH intermediate is the most optimal reaction pathway for the FAEO.

关键词： Cluster Formic acid oxidation Electrocatalysis In situ FTIR Reaction mechanism

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Estimation of the potential geographical distribution of a new potato pest(Schrankia costaestrigalis) in China under climate change

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Journal of Integrative Agriculture 2023年第8期22卷 2441-2455页

作者： XIAN Xiao-qing ZHAO Hao-xiang guo Jian-yang ZHANG Gui-fen LIU Hui LIU Wan-xue WAN fang-hao State Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant ProtectionChinese Academy of Agricultural SciencesBeijing100193P.R.China The National Agro-Tech Extension and Service Center Beijing 100193P.R.China

Global food security is threatened by the impacts of the spread of crop pests and changes in the complex interactions between crops and pests under climate *** costaestrigalis is a newly-reported potato pest in southern ***-warning monitoring of this insect pest could protect domestic agriculture as it has already caused regional yield reduction and/or quality decline in potato *** research aimed to confirm the potential geographical distributions(PGDs)of *** in China under different climate scenarios using an optimal MaxEnt model,and to provide baseline data for preventing agricultural damage by *** findings indicated that the accuracy of the optimal MaxEnt model was better than the default-setting model,and the minimum temperature of the coldest month,precipitation of the driest month,precipitation of the coldest quarter,and the human influence index were the variables significantly affecting the PGDs of *** highly-and moderately-suitable habitats of *** were mainly located in eastern and southern *** PGDs of *** in China will decrease under climate *** conversion of the highly-to moderately-suitable habitat will also be significant under climate *** centroid of the suitable habitat area of *** under the current climate showed a general tendency to move northeast and to the middle-high latitudes in the *** agricultural practice of plastic film mulching in potato fields will provide a favorable microclimate for *** in the suitable *** attention should be paid to the early warning and monitoring of *** in order to prevent its further spread in the main areas in China’s winter potato planting regions.

关键词： climate change potential geographical distribution crop insect pests Schrankia costaestrigalis MaxEnt model

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基于数据挖掘的针刺治疗紧张性头痛取穴规律分析

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环球中医药 2024年第8期17卷 1537-1544页

作者：虞兵兵王婷婷方俊霖郭云黄英如郑凯重庆医科大学中医药学院 400050 重庆医科大学护理学院 400050 重庆医科大学附属铜梁区中医院骨伤科

目的通过数据挖掘技术分析针刺紧张性头痛的取穴规律,为临床治疗提供参考。方法计算机检索中国知识资源总库(CNKI)、维普资讯中文期刊服务平台(VIP)、中国学术期刊数据库(万方数据)收录的自建库起至2023年3月31日有关针刺治疗紧张性头... 详细信息

目的通过数据挖掘技术分析针刺紧张性头痛的取穴规律,为临床治疗提供参考。方法计算机检索中国知识资源总库(CNKI)、维普资讯中文期刊服务平台(VIP)、中国学术期刊数据库(万方数据)收录的自建库起至2023年3月31日有关针刺治疗紧张性头痛的临床文献。共检索到783篇文献,经过筛选最终纳入符合纳排标准文献90篇。采用Excel表格建立数据库,统计并分析符合纳排标准文献的腧穴选取频次、归经、所属部位、特定穴位等方面的应用规律。运用SPSS 25.0软件对出现频次≥16的腧穴进行聚类分析及因子分析,并绘制树状图、冰状图及旋转空间载荷图。结果针刺治疗紧张性头痛的临床应用中风池为最高频腧穴,紧随其后的是百会、太阳、头维等腧穴;腧穴归经主要是督脉、足少阳胆经、足阳明胃经;所属部位应用最多的为头面部,其次为下肢、上肢;特定穴以原穴最为常用,之后是输穴与络穴。聚类分析得到3类腧穴配伍关联聚类处方,足三里、丰隆、三阴交、太冲为一类;印堂、神门为一类;风池、太阳、头维、百会、合谷、四神聪、率谷为一类。因子分析共提取5个腧穴配伍单元公因子。分别为F1(丰隆、三阴交、太冲、足三里)、F2(风池、头维、百会)、F3(四神聪、率谷)、F4(印堂、神门)、F5(合谷、太阳)。结论针刺治疗紧张性头痛取穴以近部取穴为主,主要是百会、太阳、头维等穴位,同时重视循经取穴、远端取穴与特定穴的使用,常配合辨经、辨证理念治疗。通过数据挖掘针刺治疗紧张性头痛取穴规律,为其临床应用及基础研究提供参考。

关键词：针刺紧张性头痛肌收缩性头痛头风取穴规律经络文献研究

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Cryoablation Combined with TACE for Treating Large Hepatocellular Carcinoma： Tumor Load and Cellular Immunity

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Chinese Journal of Clinical Oncology 2005年第6期2卷 842-848页

作者： Haipeng Yu Lanlan Yang Zhi guo Wenge Xin fang Liu Xiuying guo Baoguo Li Department of Interventional Therapy Cancer Institute ＆ Hospital of TianjinMedical University Tianjin 300060China. Department of Digestive Medicine TheSecond Hospital of Jilin UniversityChangchun 130041 China.

OBJECTIVE To study the effectiveness on the tumor load and cellular immune function of percutaneous cryoablation （argon-helium cryoablative system, AHCS） combined with transarterial chemoembolization （TACE） for treating large hepatocellular carcinomas （HCCs） with diameters over 10 ca. METHODS A total of 48 HCC patients were treated with AHCS after TACE. Tumor sizes ranged from 10 to 14 cm. All cases were a hypervascular type. There were 38 Child A cases and 10 Child B cases. Forty were AFP positive and 8 negative. The patients were randomized with therapy group consisting of 26 cases and the control group 22 cases. The therapy group received AHCS 4 weeks following TACE treatment. Reexamination included pathology, tumor markers, T-lymphocyte subgroup levels and computed tomography or MRI. The necrosis rate of the tumor load was calculated by Cavalieri＇s theory. EORTC QLQ-C30 was used in quality of life evaluation. RESULTS The average tumor-load reduction rate （necrosis rate） was 8.07% after TACE, and 28.65% after AHCS. Coagulation necrosis was produced in the target area. The tumor markers deceased significantly after AHCS. Tumor-load reduction after AHCS was more significant than after TACE. Suppression of cellular immunity after TACE was significant. In contrast, CD3^＋, CD4^＋ and NK increased after AHCS and an abnormal T-lymphocyte distribution was corrected. Quality of life after AHCS increased according to the EORTC QLQ-C30 evaluation. No severe complications occurred. CONCLUSION Percutaneous AHCS cryoablation after TACE reduced the tumor load in the short term. At the same time, cellular immune function was increased after AHCS. TACE was critical in increasing the therapeutic efficacy of AHCS because of its embolisation of blood vessels preventing a Flow Effect. Reduction of the tumor load in the short term may conduce to increase cellular immunity. Percutaneous AHCS cryoablation combined with TACE can reduce the tumor load, improve cellular immunity and increas

关键词： cryoablation transarterial chemoembolization tumor load cellular immunity hepatocellular carcinoma.

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新能源发展对能源强度和经济增长的影响

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系统工程理论与实践 2013年第11期33卷 2795-2803页

作者：方国昌田立新傅敏孙梅江苏大学能源发展与环境保护战略研究中心镇江212013 盐城工学院基础教学部盐城224051

新能源的开发和利用是解决能源短缺、环境污染等问题的重要途径,是发展低碳经济的有效动力源.本文从非线性动力学入手,将新能源纳入节能减排演化系统,分析了新能源对能源强度和经济增长的影响.借助李雅普诺夫指数和分岔图分析了系统的... 详细信息

新能源的开发和利用是解决能源短缺、环境污染等问题的重要途径,是发展低碳经济的有效动力源.本文从非线性动力学入手,将新能源纳入节能减排演化系统,分析了新能源对能源强度和经济增长的影响.借助李雅普诺夫指数和分岔图分析了系统的动力学行为,得到了新能源约束下的节能减排吸引子.借助神经网络,得到了满足中国情况实际系统的参数.对实际系统进行实证分析,得到一些与实际符合的分析结果.结果表明:依靠新能源自身发展或单纯加大对新能源的经济投入,并不能很好地控制能源强度.当经济投入过大时会对经济发展带来很大的阻碍作用,甚至给经济带来致命的影响.加大包括新能源在内的节能减排等的综合投入,可以很好地降低能源强度,当综合投入加大时,开始对经济的阻碍也大,但是随着系统的进一步发展,当新能源发展成熟时,这种投入对经济的促进作用也大.利用数值仿真来展示这些结果.

关键词：节能减排新能源能源强度经济增长

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热等静压对激光选区熔化成形GH4169合金组织与高温性能的影响

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航空材料学报 2024年第1期44卷 84-92页

作者：赵海生房立家刘欢窦开沁中国航发北京航空材料研究院焊接与塑性成形研究所北京100095 航发优材(镇江)增材制造有限公司技术部江苏镇江212132

GH4169合金通常于高温环境下服役,增材制造的GH4169合金高温性能往往低于锻件标准,影响其服役安全性,有必要通过热处理手段提高其安全性。采用激光选区熔化技术(SLM)制备GH4169合金,利用金相显微镜(OM)、扫描电子显微镜(SEM)、X射线能... 详细信息

GH4169合金通常于高温环境下服役,增材制造的GH4169合金高温性能往往低于锻件标准,影响其服役安全性,有必要通过热处理手段提高其安全性。采用激光选区熔化技术(SLM)制备GH4169合金,利用金相显微镜(OM)、扫描电子显微镜(SEM)、X射线能谱分析(EDS)等表征手段,探究热等静压热处理工艺对SLM成形GH4169合金微观组织、高温拉伸性能和高温持久性能的影响。结果表明:与常规热处理工艺相比,经过热等静压处理后的GH4169合金晶粒为等轴晶,气孔基本消失,Laves相基本消失,短棒状δ相在晶界处更加连续,颗粒状γ''相在晶粒内部大量析出,晶粒得到明显细化。热等静压处理后GH4169合金在扫描方向与沉积方向上的650℃抗拉强度分别为1053 MPa和1051 MPa,断后伸长率分别为8.6%和8.5%;高温持久时间分别为1620 min和2065 min,高温持久时间分别提升了90倍和30倍。高温抗拉强度和高温持久时间均高于锻件的性能指标,断后伸长率尚未超过锻件标准。

关键词：激光选区熔化成形 GH4169 热等静压组织演变高温性能

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TRIM65 E3 ligase targets VCAM-1 degradation to limit LPS-induced lung inflammation

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Journal of Molecular Cell Biology 2020年第3期12卷 190-201页

作者： Yong Li Xuan Huang fang guo Tianhua Lei Shitao Li Paula Monaghan-Nichols Zhisheng jiang Hong-Bo Xin mingui Fu Department of Biomedical Science School of MedicineUniversity of Missouri-Kansas CityKansas CityMO 64108USA Institute of Translational Medicine Nanchang UniversityNanchang 330031China Institute of Cardiovascular Diseases Department of PathophysiologyHengyang Medical CollegeUniversity of South ChinaHengyang A21001China Department of Physiological Sciences Center for Veterinary and Health sciencesOklahoma State UniversityStillwaterOK 7A078USA

Although the adhesion molecules-mediated leukocyte adherence and infiltration into tissues is an important step of inflammation,the post-translational regulation of these proteins on the endothelial cells is poorly ***,we report that TRIM65,an ubiquitin E3 iigase of tripartite protein family,selectively targets vascular cell adhesion molecule 1(VCAM-1)and promotes its ubiq-uitination and degradation,by which it critically controls the duration and magnitude of sepsis-induced pulmonary ***65 is constitutively expressed in human vascular endothelial *** TNFa-induced endothelial activation,the protein levels ofTRIM65 and VCAM-1 are inversely *** of wild-type TRIM65,but not expression of aTRIM65 mutant that lacks E3 ubiquitin ligase function in endothelial cells,promotes VCAM-1 ubiquitination and degradation,whereas small interference RNA-mediated knockdown of TRIM65 attenuates VCAM-1 protein *** experiments show that TRIM65 directly interacts with VCAM-1 protein and directs its polyubiquitination,by which TRIM65 controls monocyte adherence and infiltration into tissues during ***,TRIM65-deficient mice are more sensitive to lipopolysaccharide-induced death,due to sustained and severe pulmonary *** together,our studies suggest that TRIM65-mediated degradation of VCAM-1 represents a potential mechanism that controls the duration and magnitude of infiammation.

关键词： TRIM65 VCAM-1 endothelial activation lung inflammation ubiquitination

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Nkx2-1:a novel tumor biomarker of lung cancer

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2012年第11期13卷 855-866页

作者： LiYANG min LIN Wen-jing RUAN Liang-liang DONG En-guo CHEN Xiao-hong WU Ke-jing YING Department of Respiratory Medicine the First Affiliated Hospital of Zhejiang Chinese Medical University Hangzhou 310006 China Department of Radiology the Third Affiliated Hospital of Zhejiang Chinese Medical University Hangzhou 310005 China Key Laboratory of Biotherapy of Zhejiang Province Department of Respiratory Medicine Sir Run Run Shaw Hospital School of Medicine Zhejiang University Hangzhou 310016 China

Nkx2-1 (Nkx homeobox-1 gene), also known as TTF-1 (thyroid transcription factor-1), is a tissue-specific transcription factor of the thyroid, lung, and ventral forebrain. While it has been shown to play a critical role in lung development and lung cancer differentiation and morphogenesis, molecular mechanisms mediating Nkx2-1 cell- and tissue-specific expression in normal and cancerous lungs have yet to be fully elucidated. The recent identification of prognostic biomarkers in lung cancer, particularly in lung adenocarcinoma (ADC), and the different reactivity of patients to chemotherapeutic drugs have opened new avenues for evaluating patient survival and the development of novel effective therapeutic strategies. The function of Nkx2-1 as a proto-oncogene was recently characterized and the gene is implicated as a contributory factor in lung cancer development. In this review, we summarize the role of this transcription factor in the development, diagnosis, and prognosis of lung cancer in the hope of providing insights into the utility of Nkx2-1 as a novel biomarker of lung cancer.

关键词： Nkx2-1 TTF-1 Lung cancer Biomarker Diagnosis Prognosis Therapy

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