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Cloning and expression of heat shock protein genes in two whitefly species in response to thermal stress

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Cloning and expression of heat shock protein genes in two wh...

第二届全国生物入侵学术研讨会

作者： hao Yu,fang-hao wan State Key Laboratory for Biology of Plant Diseases and Insect Pests,Institute of Plant Protection, Chinese Academy of Agricultural Sciences,Beijing 100081,PR China. Center for Management of Invasive Alien Species,Ministry of Agriculture,Beijing 100081,PR China

Two whitefly species,Trialeurodes vaporariorum and Bemisia tabaci biotype B were shown to have different temperature tolerance and seasonal *** determine whether this variation in thermal tolerance is related to different expression patterns of heat shock proteins(Hsps) genes during temperature stress,we obtained complete cDNA sequences for hsp90,hsp70,and hsp20, and analyzed their expression profiles across temperature gradients by real-time quantitative *** full length cDNAs were cloned and sequenced from these two *** full-length cDNAs of hsp90s contain 2166,2157 bp open reading frame(ORF) which encode proteins with calculated molecular weights of 83013 and 82857 *** 1947,1959 bp ORFs of whiteflies hsp70s comprise 649 and 653 aa with the calculated masses of 70885 and 71008 Da in *** and ***, *** complete cDNAs of hsp20 of *** and *** contains 585 bp ORFs and deduced amino acid sequences calculated molecular weights 21559 and 21539 Da,respectively. The hsps expression profiles results showed temperatures for onset(T

关键词： heat shock protein expression profile temperature tolerance whitefly

Comparison of efficiency of mass-rearing of the Cydia pomonella(L.)on three artificial diets

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Comparison of efficiency of mass-rearing of the Cydia pomone...

第三届全国生物入侵大会

作者： Jian-Ru Shen(*,2),wan-Xue Liu~1,fang-hao wan~1 1 State Key Laboratory for Biology of Plant Diseases and Insect Pests,Institute of Plant Protection, Chinese Academy of Agricultural Sciences,Beijing 100094,China 2 College of Plant Protection,Southwestern University,Chongqing,400715,China

Codling moth,Cydia pomonella(L.)(Lepidoptera:Olethreutidae),is the most serious pest of fruit orchards worldwide and one of the most important quarantine *** advanced method of controlling the codling moth is by the sterile insect release technique,which requires mass-reared insects.A key component of mass rearing programs is choice of diet for the *** based on

关键词： Cydia pomonella(L.) Artificial diets Efficiency Survival rate of neonate Egg production

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害虫遗传防治的研究历史与现状

生物安全学报 2015年第2期24卷 81-93,74页

作者：严盈万方浩中国农业科学院植物保护研究所植物病虫害生物学国家重点实验室北京100193 Department of Entomology North Carolina State University Genetic Engineering and Society Center and W. M. Keck Center for Behavioral Biology North Carolina State University 农业部外来入侵生物预防与控制研究中心北京100193

作为防治或根除重大害虫最为有效的手段之一,害虫遗传防治在世界范围内被广泛采用并取得了巨大成功。本文综述了不育昆虫技术、雌性致死系统和昆虫显性致死技术等经典害虫遗传防治策略的发展历史、技术特点和应用情况。近年来,许多新的... 详细信息

作为防治或根除重大害虫最为有效的手段之一,害虫遗传防治在世界范围内被广泛采用并取得了巨大成功。本文综述了不育昆虫技术、雌性致死系统和昆虫显性致死技术等经典害虫遗传防治策略的发展历史、技术特点和应用情况。近年来,许多新的分子生物手段被不断提出并整合到害虫遗传防治策略中,包括归巢核酸内切酶基因、锌指核酸酶、转录激活因子样效应因子核酸酶、CRISPR/Cas9系统、Medea元件、Killer-Rescue系统、Wolbachia-细胞质不亲和性系统等。基于这些新的工具手段,许多国家已经在不同程度上启动了下一代害虫遗传防治项目。而我国在该领域的研究相对薄弱,需要在借鉴国外成功经验的同时,进一步加强害虫遗传防治的基础和应用研究,从而实现本地有害生物的可持续治理和外来入侵生物的有效狙击,确保我国未来的粮食和生态安全。

关键词：不育昆虫技术大规模饲养染色体移位遗传定性品系转座子

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Altered arbuscular mycorrhizal fungi communities favoring invasive weed Ageratina adenophora Sprengl competition over natives

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Altered arbuscular mycorrhizal fungi communities favoring in...

第三届全国生物入侵大会

作者： Wen-qing Yu~(1,2),Li-li Zhang~3,fang-hao wan~1,wan-xue Liu~(1*) 1 State Key Laboratory for Biology of Plant Diseases and Insect Pests,Institute of Plant Protection, Chinese Academy of Agricultural Sciences,Beijing 100094 2 Heilongjiang Academy of Land Reclamation Sciences,Jiamusi,Heilongjiang,154007

To investigate the difference of Arbuscular mycorrhizal fungi(AMF) community between exotic weed Ageratina adenophora invaded soil and native weeds dominated soil,and to inspect the feedback of the different AMF community to competitive interaction of the exotic weed with native plants,we conducted field and glasshouse *** field experiments showed that the aboundance of Glomus geosporum,Acaulospora dilatata and Acaulosporafoveata in exotic *** dominated soil

关键词： AM symbiosis AMF Feedback to plants Competitive interactions Community diversity Exotic plant Mechanism of invasion Plant-plant interactions Plant-soil interactions

Positive effect of Mo on mechanical properties of a Ni-42W-10Co-x Mo medium heavy alloy

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China Foundry 2025年

作者： Guo-xin Cao Jian-xin Dong Yong-sheng He He Jiang fang hao Zhi-hua Nie Teng-fei Ma Bao-quan Fu School of Materials Science and Engineering University of Science and Technology Beijing Xi'an Superalloy Technology Co. Ltd. School of Materials Science and Engineering Beijing Institute of Technology Key Laboratory of Air-driven Equipment Technology of Zhejiang Province Quzhou University

In this study,a novel Ni-W-Co-Mo medium heavy alloy (MHA) was designed to improve its mechanical strength via Mo *** the Ni-42W-10Co-xM o alloy series,where x represents the weight percent of Mo and varies between 0,1,2,5,and 10,the microstructure transitions from a dendritic structure to a hypoeutectic structure as the Mo content increases from 0 to 5wt.%.Moreover,as the Mo content increases from 0to 10wt.%,the distribution of theμ-phase shifts from being individually dispersed to forming aggregates,and its volume fraction rises from 0.5%to 7.9%.Notably,theμ-phase evolves into an eutectic microstructure,which helps in minimizing the segregation of *** change is accompanied by a substantial enhancement in mechanical properties;specifically,the compressive yield strength at room temperature increases from 350 MPa to 646 MPa,indicating a significant 85%***,the microhardness increases from 230 HV to 304 *** dynamics simulations further reveal that the strengthening mechanism of Ni-42W-10Co-xM o alloys is Mo-induced solid solution strengthening and precipitation strengthening.

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Design and testing research of LiDAR for detecting atmospheric turbulence

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Optoelectronics Letters 2025年第3期21卷 172-176页

作者： QIU Duoyang LI Xianyang YANG hao ZHU Xiaomeng fang Zhiyuan XU Xiang School of Advanced Manufacturing Engineering Hefei University Anhui Provincial Engineering Technology Research Center of Intelligent Vehicle Control and Integrated Design Technology Hefei University Science Island Branch of Graduate School University of Science and Technology of China School of Mechanical and Energy Engineering Ningbo Tech University Key Laboratory of Atmospheric Optics Anhui Institute of Optics and Fine Mechanics Chinese Academy of Sciences

Atmospheric turbulence is an important parameter affecting laser atmospheric transmission. This paper reports on a self-developed atmospheric turbulence detection Li DAR system (scanning differential image motion Li DAR(DIM-Li DAR) system). By designing and simulating the optical system of atmospheric turbulence detection Li DAR,the basic optical imaging accuracy has been determined.

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Competition effect of Cogon grass,Imperata cylindrica to Crofton weed,Ageratina adenophora

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Competition effect of Cogon grass,Imperata cylindrica to Cro...

第三届全国生物入侵大会

作者： Fu-rong Gui~1,Zheng-yue Li~1,fang-hao wan~(2*) 1.College of Plant Protection,Yunnan Agricultural University,Kunming 650201,China 2.Institute of Plant Protection,Chinese Academy of Agricultural Sciences,Beijing 100081,China

Invasive alien species are the most important indicators of environmental change worldwide;it may cause environmental degradation,biodiversity loss,food and water shortage,and high possibility and severity of natural *** in management of invasive species has grown rapidly in the recent years,and it is a good way to replace invaded plant by utilizing valuable native *** competition effects between the native Gramineous plant,Cogon grass,Imperata cylindrical and the invasive Compositae plant,Crofton weed,Ageratina adenophora were analyzed in the present study to provide basis for control of this invasive plant The results showed that,there was significant mutual antagonism between *** and *** relative competitive ability of *** was higher than that of ***,which heavily suppressed the growth of *** by shoot *** to ***,the seedlings of *** were germinated faster, and its plant height and average leaf area were *** culture with *** may restrain growth and tillering of ***,and the plant height,average leaf area and chlorophyll content were 49%,72%and 77%of those in monoculture,*** there no significant influence on *** for mixed culture with ***,furthermore,the tillering of *** can be *** relative yield(RY) of *** was significantly higher than those of A. adenophora,and the relative yield total(RYT) was significantly lower than *** relationships mentioned above may become more obvious with the prolonged time of mixed culturing.

关键词： Ageratina adenophora Imperata cylindrical Relative yield Competition effect Replacement control

Differential expression of Hsp70 mRNA may underline elevated heat-resistance in Bemisia tabaci B-biotype females

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Differential expression of Hsp70 mRNA may underline elevated...

第一届全国生物入侵学术研讨会

作者： Zhi-Chuang L~a,fang-hao wan~(a,b,*) (a State Key Laboratory for Biology of Plant Diseases and Insect Pests,Institute of Plant Protection, CAAS,Beijing 100081,PR China.b Center for Management of Invasive Alien Species,Ministry of Agriculture,Beijing 100081,PR China)

The survivals of *** females and males were examined with heat *** amount of Hsp70 mRNA in the two *** sexes under heat shocks and under a range of lower temperatures was quantified by TaqMan real-time RT-PCR *** results obtained are presented below: *** females were significantly more heat-resistant than males at 44℃. ***70 mRNA in *** females increased gradually from 25 to 35℃,and the increase accelerated sharply from 37.5℃and reached a peak at 42℃,and it then decreased at 44℃. ***70 mRNA in *** males increased continuously from 25℃until it reached a peak at 44℃. ***70 mRNA expression in *** females and males displayed no significant difference at 25℃. Hsp70 mRNA expression in *** males was significantly higher than that in the females in a range of 30-35℃and 44℃,and was significantly lower than that in the females at 40℃and 42℃. Our results indicate that *** females were more heat-resistant than males,which was mediated, at least in part,via the activation of Hsp70.

关键词： Bemisia tabaci Heat resistance Hsp70 mRNA Sexual dichotocarpism TaqMan real-time RT-PCR

Plant protection: Current status, progress and challenges in China

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Journal of Integrative Agriculture 2019年第4期18卷 703-704页

作者： wan fang-hao State Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant Protection Chinese Academy of Agricultural Sciences Agricultural Genomics Institute at Shenzhen Chinese Academy of Agricultural Sciences

China is one of the countries in the world that is severely damaged by crop diseases,pests,and *** are more than 1 700 species of agricultural pests occurring annually,53 of which are on the list of the 100 worst pests in the world(Chen and wang 2014;Wu 2018).Currently,China is still greatly challenged by a growing problem of agricultural pests that th reate n the economy and health and pose ecological risks underthe conditions of global climate change,economic integration,crop structure adjustment and so *** protect!on agai nst crop diseases,in sect pests and noxious weeds is necessary to ensure high-quality crop production and food ***,traditional strategies of plant protect!on have mainly involved chemical pesticides.

关键词： Plant protection crop diseases agricultural pests

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