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室内空气环境的数值模拟与通风模式的评估

水动力学研究与进展（A辑） 2004年第Z1期19卷 904-911页

作者：唐振朝詹杰民中山大学应用力学与工程系广州510275

　采用CFD技术对存在隔板及持续释放二氧化碳的污染源的开放式办公室进行了数值模拟,计算分析了两种通风模式下污染物在室内的分布状况以及空气年龄的变化情况,其中空气年龄的计算是利用Fluent软件提供的自定义函数接口技术引入了对应... 详细信息

　采用CFD技术对存在隔板及持续释放二氧化碳的污染源的开放式办公室进行了数值模拟,计算分析了两种通风模式下污染物在室内的分布状况以及空气年龄的变化情况,其中空气年龄的计算是利用Fluent软件提供的自定义函数接口技术引入了对应的输运方程来实现。根据模拟结果对开放式办公室内如何选择通风模式提出了建议。结果显示当污染源位置发生变化时,置换型通风模式与混合型通风模式在通风率和换气率的表现上也会发生变化。当室内隔板数目增加的时候,置换型通风模式在房间的整体通风效果上要稍优于混合型通风模式。

关键词： CFD 隔板通风模式空气质量

Studies on mechanism of Sialy Lewis-X antigen in liver metastases of human colorectal carcinoma

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World Journal of Gastroenterology 2001年第3期7卷 425-430页

作者： Xiao Wei Li~1 Yan Qing Ding~1 Jun jie Cai~1 Shao Qing Yang~2 Lian Bing An~3.Dong fang Qiao~3.~1department of Pathology,Nanfang Hospital of the First Military Medical university,guangzhou 510515,Guangdong Province,china.~2The Northern Hospital of PLA,Shenyang 110015,Liaoning Province,china.~3.2} of Electronmicroscopy,First Military Medical university,guangzhou 510515,Gangdong Province,china.r.Xiao Wei Li graduated from the First Military Medical university with a MM degree in 1999.Physician in Charge of pathology,having 6 papers published. department of Pathology Nanfang Hospital of the First Military Medical University Tonghe Guangzhou 510515 Guangdong Province China.

INTRODUCTIONSialyl Lewis-X antigen ,correlated with carcinoma, is a group of carbohydrate antigen containing oligosaccharide expressed of embryonic tisue and glycoproteins on cell surface of embryonic tissue[1].The SLeX antigen located on cell surface is synthesized principally by two enzymes ,al ,3.ucosyltransfrease and a2, *** adults ,SLeX antigen is expressed principally on the surfaces of granulocytic cells and some tumor cells .

关键词： Animals Antibodies, Monoclonal Antigens, CD15 Cell Adhesion Colorectal Neoplasms E-Selectin Endothelium, Vascular Flow Cytometry HT29 Cells Humans Immunohistochemistry In Situ Hybridization Liver Neoplasms Mice Mice, Inbred BALB C Mice, Nude Microscopy, Electron Microscopy, Electron, Scanning N-Acetylneuraminic Acid RNA, Messenger Research Support, Non-U.S. Gov't Tumor Cells, Cultured Umbilical Veins

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黄曲霉毒素解毒酶的固定化及其性质的研究

生物工程学报 2003年第5期19卷 603-607页

作者：刘大岭姚冬生黄炳贺谢春芳梁郁强马林暨南大学生命科技学院生物系广州510632 广东省技术开发中心广州510070 中山大学化学与化学工程学院有机所广州510275

黄曲霉毒素是农作物常见的受污染的霉菌毒素 ,毒性大 ,稳定性高 ,是潜在的肝癌致癌物 ,对人的危害较大。该毒素的解毒与去毒一直是受到关注的问题。黄曲霉毒素解毒酶对黄曲霉毒素有特殊的去毒和降解作用 ,但是该酶的稳定性离解决实际问... 详细信息

黄曲霉毒素是农作物常见的受污染的霉菌毒素 ,毒性大 ,稳定性高 ,是潜在的肝癌致癌物 ,对人的危害较大。该毒素的解毒与去毒一直是受到关注的问题。黄曲霉毒素解毒酶对黄曲霉毒素有特殊的去毒和降解作用 ,但是该酶的稳定性离解决实际问题尚有一段距离。报道了对黄曲霉毒素解毒酶的固定化 ,并对固定化处理后酶的稳定性、性质、催化活性、解毒活性进行了测定。结果表明 ,通过固定化操作酶的解毒活性被保留下来 ,酶的酸碱稳定性、热稳定性。

关键词：黄曲霉毒素解毒酶固定化稳定性催化活性

TIME-DEPENDENT PLASTICIRY PRODUCED BY LTP OF C-FIBER EVOKED FIELD POTENTIALS IN SPINAL DORSAL HORN

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神经解剖学杂志 2001年第B7期17卷 123-124页

作者： Hong-Mei ZHANG~1, Ying-jie QI~2, Xu-Ying XIANG~1, Xian-Guo LIU~1 ~1 department of physiology, Sun Yat-sen university of Medical Sciences, 74 {3. Rd 2, guangzhou 510089,~2 Xinxiang Medical College, Henan

Aim: Hippocampal long-term potentiation (LTP), an activity-dependent plasticity in synaptic transmission, is believed as a fundamental mechanism of learning and memory. This form of plasticity also exists in spinal dorsal horn. Our previous work showed that electrical as well as natural noxious stimulation of afferent C-fibers or acute injury of the sciatic nerve produces LTP of C-fiber evoked field potentials in spinal dorsal horn.

关键词：脊髓背角 LTP C-纤维突触传导电刺激时间依赖性可塑性

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数值水池的一种有效的数值消波方法

水动力学研究与进展（A辑） 2002年第1期17卷 46-52页

作者：詹杰民李毓湘中山大学应用力学与工程系广东广州510275 香港理工大学土木及结构工程系

本文通过将波高所满足的方程分解成等效的两个 x和 y方向的一维方程 ,提出了一种在求解 Boussinesq型方程中进行数值消波的有效方法 ,在求解方程时采用了一种精度较高的预报校正线迭代方法。给出的方法用有实验结果的多向不规则波例子... 详细信息

本文通过将波高所满足的方程分解成等效的两个 x和 y方向的一维方程 ,提出了一种在求解 Boussinesq型方程中进行数值消波的有效方法 ,在求解方程时采用了一种精度较高的预报校正线迭代方法。给出的方法用有实验结果的多向不规则波例子进行了验证。数值结果与实验结果相当吻合。本文的方法解决了波浪仿真中的关键技术

关键词：数值水池数值消波 Boussinesq型方程波浪仿真多向不规则波波高

The mechanisms underlying that injury of skin afferents does not produce neuropathic pain:the role of BDNF

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The mechanisms underlying that injury of skin afferents does...

The differences between hippocampal LTP and spinal LTP

中国神经科学学会第四次会员代表大会暨第八届全国学术会议

作者： Li-Jun ZHOU Wen-jie REN Yi ZHONG Tao YANG Xian-Guo LIU~(1 *) Pain Research Center and department of Physiology zhongshan Medicine School of Sun Yat-sen {3. guangzhou 510089 china

＜正＞Objective Although a large body of evidence has shown that peripheral nerve injury usually induces neuropathic pain,there are also clinical studies demonstrating that injury of the sural nerve,which mainly innervates skin,fails to do *** underlying mechanism,however,is largely ***-derived neurotrophic factor（BDNF）,which is essential for late-phase LTP（L-LTP） in hippocampus,plays an important role in sensory neurotransmission in nociceptive pathways at both spinal and supraspinal *** recent work has demonstrated that BDNF is critical for spinal L-LTP of C-fiber evoked field *** the present study,we investigated whether electrical stimulation or injury of the tibial nerve,innervating both muscle and skin or the sural nerve,innervating mainly skin has different effects on producing neuropathic pain and on inducing LTP of C-fiber-evoked field potentials in spinal dorsal *** role of BDNF in the spinal LTP induced by electrical stimulation of the tibial nerve or the sural nerve was *** Pain behavior test,field potential recorded by electrophysiological in vivo and with immunohistochemistry by injection of Fluorogold. Results In the present work we found that injury of the tibial nerve innervating both muscle and skin but not of the sural nerve produced a lasting mechanical allodynia in adult rats;High frequency stimulation（HFS） or injury of the tibial nerve induced L-LTP of C-fiber evoked field potentials in spinal dorsal horn,lasting for more than 5 h,while HFS or injury of the sural nerve only induced early-phase LTP（E-LTP）,lasting less than 3.***,HFS of the tibial nerve induced L-LTP of C-fiber responses evoked by stimulation of the sural nerve and the heterosynaptic L-LTP was completely prevented by spinal application of TrkB-Fc（a BDNF scavenger）.Spinal application of BDNF at a dose as low as 10 pg/ml enabled HFS of the sural nerve to produce homosynaptic ***,our immunohistochemical results re

关键词： sural nerve brain-derived neurotrophic factor neuropathic pain long-term potentiation spinal dorsal horn

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The differences between hippocampal LTP and spinal LTP

芒果生长素反应因子类蛋白的cDNA克隆和表达

中国神经科学学会第九届全国学术会议暨第五次会员代表大会

作者： Xian-Guo LIU~*,Yong LIU,Wen-jie REN,Jun WANG,He-Qun ZHU Pain Research Center and department of Physiology,zhongshan School of Medicine,Sun Yat-Sen {3., guangzhou 510080,china

＜正＞Long-term potentiation（LTP） in hippocampus is a synaptic model of memory storage,while LTP of C-fiber synapses in spinal dorsal horn underlies chronic pain produced by nerve injury and ***,it is clinically important to explore similarities and differences in the mechanisms of hippocampal LTP and spinal LTP. LTP in both regions is prevented by blockage of NMDA *** protein kinases,such as PKA,PK.C, CaMKII and ERK are critically involved in LTP induction and early-phase maintenance in both hippocampus and in spinal dorsal *** drugs targeting at these molecules may not only inhibit chronic pain but also impair memory. The striking difference is that activation of microglia and subsequent over-expression of pro-inflammatory cytokines, such as tumor necrosis factor-alpha（TNF-α）,inhibit LTP in hippocampus but facilitate LTP in spinal dorsal horn. Interestingly,both the inhibition of hippocampal LTP and facilitation of spinal LTP are mediated by activation of nuclear factor kappaB,p3. MAPK and *** we found that peripheral nerve injury,which induces spinal LTP,inhibited hippocampal LTP and impaired working memory by up-regulation of TNF-αin spinal cerebral fluid and in hippocampal *** effect was prevented by pharmacological inhibition of TNF-αor genetic deletion of TNF receptor *** data suggested that over-expression of the pro-inflammatory cytokine in CNS might lead to chronic pain and memory deficit,***,inhibition of TNF-αand its downstream molecules may relieve chronic pain and improve memory function as well.

关键词： LTP hippocampus spinal dorsal horn TNF-a memory chronic pain

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生物工程学报 2004年第1期20卷 59-62页

作者：肖洁凝黄学林黄霞李筱菊中山大学生命科学学院基因工程教育部重点实验室

通过SSH法获得了一个与不定根形成相关的差异表达的cDNA片段 ,其推导的氨基酸序列与拟南芥的生长素反应因子 (ARF)类蛋白具有较大的同源性 ,因此将它命名为MiARF。用所设计的基因特异引物进行 3.RACE扩增获得包含完整读码框架 (ORF)的M... 详细信息

通过SSH法获得了一个与不定根形成相关的差异表达的cDNA片段 ,其推导的氨基酸序列与拟南芥的生长素反应因子 (ARF)类蛋白具有较大的同源性 ,因此将它命名为MiARF。用所设计的基因特异引物进行 3.RACE扩增获得包含完整读码框架 (ORF)的MiARF1(GenBank登录号为AY2 5 5 70 5 )和MiARF2 (GenBank登录号为AY3. 0 80 8)。MiARF1全长为 3. 72bp ,其中 ,ORF含 2 5 2 3.p ,5′非翻译区 (5′UTR)含 2 85bp ,3.非翻译区 (3.UTR)含 4 6 4bp。由该序列所推导的氨基酸序列与拟南芥ARF2 (BAB10 16 2 )的ID值为 6 4 % ,E值为 0 ,在DNA结合区域 (DBD)、III和IV区域的同源性更高 ,ID值均大于 80 %。MiARF2cDNA全长为 14 74bp ,其中ORF含 981bp ,5′非翻译区含 2 85bp ,3.非翻译区含 2 0 8bp ,由该序列所推导的氨基酸序列与拟南芥ARF2 (BAB10 16 2 )的ID值为 84 % ,E值为e- 1 51 。MiARF2仅具有DBD保守区并与MiARF1的基本相同 ,但缺乏III和IV区域。VirturalNorthern杂交表明 :MiARF2在生根的组织中表达水平高 ,而在非生根的组织中未见表达 ;MiARF1在生根及非生根的组织中均有表达。

关键词：芒果子叶不定根形成生长素反应因子基因 cDNA克隆表达

A multicenter prospective study on the management of hepatoblastoma in children:a report from the Chinese Children's Cancer Group

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World Journal of Pediatrics 2024年第6期20卷 590-601页

作者： Meng-jie Tang Xiao-Li Ma Xiang-Ling He Wei-Hua Pan Xiao-Hong Zhang Sha-Yi Jiang Ju Gao Fu Li Wei Yao Song Gu Wei-Ling Zhang Qiang Zhao Shi-Hao Huang Yong-Jun fang Wei Liu Hui-Zhong Niu Chun-Mei Wang Li-Rong Sun Hui Gao Yun-Peng Dai Shun-Gen Huang Zhi-Yong Zhong Xi-Ge Wang Zhong-Rong Li Liang-Chun Yang Ye-Ming Wu Huan-Min Wang Xin Sun Xiao-Jun Yuan department of Pediatric Hematology/Oncology Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of MedicineNo.1665Kongjiang RoadYangpu DistrictShanghai 200092China Medical Oncology department Pediatric Oncology CenterBeijing Children's HospitalCapital Medical UniversityNational Center for Children's HealthBeijing 100045China department of Hematology/Oncology Children's Medical CenterHunan Provincial People's HospitalThe First Affiliated Hospital of Hunan Normal UniversityChangsha 41005China department of Pediatric Surgery Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of MedicineShanghai 200092China department of Hematology/Oncology Guangzhou Women and Children's Medical CenterNational Children's Medical Center for South Central RegionGuangzhou 510623China department of Hematology Shanghai Children's HospitalShanghai Jiao Tong UniversityShanghai 200062China department of Pediatrics West China Second University HospitalSichuan UniversityChengdu 610041China department of Hematology/Oncology Qilu Children's Hospital of Shandong UniversityJinan 250022China department of Pediatric Surgery Children's Hospital of Fudan UniversityShanghai 201102China department of General Surgery Shanghai Children's Medical Center(National Children's Medical Center-Shanghai)Shanghai Jiao Tong University School of MedicineShanghai 200127China department of Pediatrics Beijing Tongren HospitalCapital Medical UniversityBeijing 100730China department of Pediatric Oncology Tianjin Medical University Cancer Institute and HospitalTianjin 300060China department of Hematology/Oncology Children's Hospital of Nanjing Medical UniversityNanjing 210008China department of Hematology/Oncology Henan Children's HospitalZhengzhou 450018China department of Pediatric General Surgery Hebei Children's Hospital of Hebei Medical UniversityShijiazhuang City 050031China Children's Hospital the First Affliated Hospital of Zhengzhou UniversityZhengzhou 450052China department of Paediatric

Background This study aimed to identify survival risk factors in Chinese children with hepatoblastoma(HB)and assess the effectiveness of the new treatment protocol proposed by the Chinese Children's Cancer Group(CCCG)in *** A multicenter,prospective study that included 3.9 patients with HB from January 2015 to June 2020 was *** demographics,treatment protocols,and other related information were *** regression models and Kaplan-Meier curve methods were *** The 4-year event-free survival(EFS)and overall survival(OS)were 76.9 and 93.5%,*** 4-year EFS rates for the very-low-risk,low-risk,intermediate-risk,and high-risk groups were 100%,91.6%,81.7%,and 51.0%,*** 4-year 0S was 100%,97.3.,94.4%,and 86.8%,*** regression analysis found that age,tumor rupture(R+),and extrahepatic tumor extension(E+)were independent prognostic factors.A total of 299 patients had complete remission,and 19 *** with declining alpha-fetoprotein(AFP)>75%after the first two cycles of neoadjuvant chemotherapy had a better EFS and OS than those≤75%.Conclusions The survival outcome of HB children has dramatically improved since the implementation of CCCG-HB-2016 ***≥8 years,R+,and E+were independent risk factors for *** with a declining AFP>75%after the first two cycles of neoadjuvant chemotherapy had better EFS and OS.

关键词： Alpha-fetoprotein Hepatoblastoma Multicenter Prospective study Survival