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7-difluoromethoxyl-5,4'-di-n-octylgenistein inhibits growth of gastric cancer cells through downregulating forkhead box M1

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World Journal of Gastroenterology 2012年第33期18卷 4618-4626页

作者： Hong-lin Xiang Fei Liu Mei-fang Quan Jian-Guo Cao yuan Lv Laboratory of Medical Engineering Medical College Hunan Normal University Changsha 410013 Hunan Province China

AIM: To investigate whether the 7-difluoromethoxyl-5, 4'-di-n-octylgenistein (DFOG), a novel synthetic genistein analogue, affects the growth of gastric cancer cells and its mechanisms. METHODS: A series of genistein analogues were prepared by difluoromethylation and alkylation, and human gastric cancer cell lines AGS and SGC-7901 cultured in vitro were treated with various concentrations of genistein and genistein analogues. The cell viability was measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The cells were incubated by DFOG at different concentrations. The growth inhibitory effects were evaluated using MTT and clonogenic assay. The distribution of the phase in cell cycle was analyzed using flow cytometric analysis with propidium iodide staining. The expression of the transcription factor forkhead box M1 (FOXM1) was analyzed by reverse transcription-polymerase chain reaction and Western blotting. The expression levelsof CDK1, Cdc25B, cyclin B and p27KIP1 protein were detected using Western blotting. RESULTS: Nine of the genistein analogues had more effective antitumor activity than genistein. Among the tested analogues, DFOG possessed the strongest activity against AGS and SGC-7901 cells in vitro. DFOG significantly inhibited the cell viability and colony formation of AGS and SGC-7901 cells. Moreover, DFOG efficaciously arrested the cell cycle in G2/M phase. DFOG decreased the expression of FOXM1 and its downstream genes, such as CDK1, Cdc25B, cyclin B, and increased p27KIP1 at protein levels. Knockdown of FOXM1 by small interfering RNA before DFOG treatment resulted in enhanced cell growth inhibition in AGS cells. Up-regulation of FOXM1 by cDNA transfection attenuated DFOG-induced cell growth inhibition in AGS cells. CONCLUSION: DFOG inhibits the growth of human gastric cancer cells by down-regulating the FOXM1 expression.

关键词： Gastric cancer 7-difluoromethoxyl-5 4'-din-octylgenistein Genistein Forkhead box M1 Therapeutic action

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Surveillance of pine wilt disease by high resolution satellite

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Journal of Forestry Research 2022年第4期33卷 1401-1408页

作者： Hongwei Zhou Xinpei yuan Huanyu Zhou Hengyu Shen lin Ma Liping Sun Guofei fang Hong Sun College of Mechanical and Electrical Engineering Northeast Forestry UniversityHarbin 150040People’s Republic of China General Station of Forest and Grassland Pest Managerment National Forestry and Grassland AdministrationShenyang 110034People’s Republic of China

Pine wilt disease caused by the pinewood nematode Bursaphelenchus xylophilus has led to the death of a large number of pine trees in *** destructive disease has the characteristics of bring wide-spread,fast onset,and long incubation *** importantly,in China,the fatality rate in pines is as high as 100%.The key to reducing this mortality is how to quickly find the infected *** proposed a method of automatically identifying infected trees by a convolution neural network and bounding box *** method rapidly locates the infected area by classifying and recognizing remote sensing images obtained by high resolution earth observation *** recognition accuracy of the test data set was 99.4%,and the remote sensing image combined with convolution neural network algorithm can identify and determine the distribution of the infected *** can provide strong technical support for the prevention and control of pine wilt disease.

关键词： Pine wilt disease Satellite remote sensing image Pest identification Convolution neural network

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Early Changes of Peripheral Blood Lymphocyte Subpopulations in Patients with Occupational 2,4-dinitrophenol Poisoning

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Biomedical and Environmental Sciences 2016年第12期29卷 909-914页

作者： JIANG Jiu Kun fang Wen GU lin Hui LU yuan Qiang Department of Emergency Medicine First Affiliated HospitalSchool of MedicineZhejiang University Cancer Institute Zhejiang Tumor Hospital

2,4-dinitrophenol （DNP）, an organic compound which frequently used in industry, is considered to have high toxicity. This study aimed to investigate the early changes of lymphocyte subpopulations in patients with occupational 2,4-DNP poisoning. Totally 9 patients with acute occupational 2,4-DNP poisoning and 30 healthy volunteers as control were enrolled. The patients received immediately comprehensive supportive treatments, including large-dose glucocorticoid and repeated hemoperfusion （HP）. The ratio of CD4＋/CD8＋ T cells were significantly higher in patients upon admission compared to healthy controls （P 〈 0.01）; however, counts of total lymphocytes, CD3＋, CD3＋CD4＋, CD3＋CD8＋, B （CD19＋）, and natural killer （NK） cells （CD16＋CD56＋） were significantly reduced （all P 〈 0.001）. The NK cell count was negatively correlated with initial plasma 2,4-DNP concentration （r = -0.750, P = 0.026）. Thus, acute occupational 2,4-DNP poisoning was accompanied by immediate complex immune cell reactions, especially NK cells might play important role in severe 2,4-DNP poisoning.

关键词： DNP Early Changes of Peripheral Blood Lymphocyte Subpopulations in Patients with Occupational 2,4-dinitrophenol Poisoning

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Marsdenia tenacissima extract suppresses A549 cell migration through regulation of CCR5-CCL5 axis, Rho C, and phosphorylated FAK

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Chinese Journal of Natural Medicines 2016年第3期14卷 203-209页

作者： lin Sen-Sen LI fang-fang SUN Li FAN Wei GU Ming ZHANG Lu-Yong QIN Song yuan Sheng-Tao New Drug Screen Center China Pharmaceutical University Nanjing Sanhome Pharmaceutical Co. Ltd.

Marsdenia tenacissima, a traditional Chinese medicine, is long been used to treat various diseases including asthma, cancer, trachitis, tonsillitis, pharyngitis, cystitis, and pneumonia. Although Marsdenia tenacissima has been demonstrated to have strong anti-tumor effects against primary tumors, its effect on cancer metastasis remains to be defined, and the molecular mechanism underlying the anti-metastatic effect is unknown. In the present study, we investigated the effects of XAP(an extract of Marsdenia tenacissima) on A549 lung cancer cell migration and explored the role of CCR5-CCL5 axis in the anti-metastatic effects of XAP. Our resutls showed that XAP inhibited A549 lung cancer cell migration and invasion in a dose-dependent manner. The protein levels of CCR5, but not CCR9 and CXCR4, were decreased by XAP. The secretion of CCL5, the ligand of CCR5, was reduced by XAP. XAP down-regulated Rho C expression and FAK phosphorylation. In conclusion, XAP inhibited A549 cell migration and invasion through down-regulation of CCR5-CCL5 axis, Rho C, and FAK.

关键词： XAP A549 cell migration CCR5-CCL5 axis Rho C FAK

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Integrated color defect detection method for polysilicon wafers using machine vision

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Advances in Manufacturing 2014年第4期2卷 318-326页

作者： Zai-fang Zhang yuan Liu Xiao-Song Wu Shu-lin Kan School of Mechatronic and Automation Engineering Shanghai University School of Mechanical and Power Engineering Shanghai JiaoTong University

For the typical color detects of polysilicon wafers, i.e., edge discoloration, color inaccuracy and color non-uniformity, a new integrated machine vision detection method is proposed based on an HSV color model. By transforming RGB image into three-channel HSV images, the HSV model can efficiently reduce the disturbances of complex wafer textures. A fuzzy color clustering method is used to detect edge discoloration by defining membership function for each channel image. The mean-value classi- fying method and region growing method are used to identify the other two defects, respectively. A vision detection system is developed and applied in the produc- tion of polysilicon wafers.

关键词： Polysilicon wafers Color defect detection Machine vision Fuzzy color clustering Region growingmethod

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DeepHGNN: A Novel Deep Hypergraph Neural Network

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Chinese Journal of Electronics 2022年第5期31卷 958-968页

作者： lin Jingjing YE Zhonglin ZHAO Haixing fang Lusheng Tibetan Information Processing and Machine Translation Key Laboratory of Qinghai Province College of Computer Qinghai Normal University Xining Urban Vocational & Technical College The State Key Laboratory of Tibetan Intelligent Information Processing and Application

With the development of deep learning,graph neural networks(GNNs) have yielded substantial results in various application fields. GNNs mainly consider the pair-wise connections and deal with graph-structured data. In many real-world networks, the relations between objects are complex and go beyond *** is a flexible modeling tool to describe intricate and higher-order correlations. The researchers have been concerned how to develop hypergraph-based neural network model. The existing hypergraph neural networks show better performance in node classification tasks and so on, while they are shallow network because of oversmoothing, over-fitting and gradient vanishment. To tackle these issues, we present a novel deep hypergraph neural network(Deep HGNN). We design Deep HGNN by using the technologies of sampling hyperedge, residual connection and identity mapping, residual connection and identity mapping bring from graph convolutional neural networks. We evaluate Deep HGNN on two visual object datasets. The experiments show the positive effects of Deep HGNN, and it works better in visual object classification tasks.

关键词： Deep neural networks Graph neural network Hypergraph neural network Deep hypergraph neural network

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Cross-Entropy Loss for Recommending Efficient Fold-Over Technique

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Journal of Systems Science & Complexity 2021年第1期34卷 402-439页

作者： WENG lin-Chen ELSAWAH A M fang Kai-Tai Division of Science and Technology Beijing Normal University-Hong Kong Baptist University United International CollegeZhuhai 519085China Department of Mathematics Faculty of ScienceZagazig UniversityZagazig 44519Egypt The Key Lab of Random Complex Structures and Data Analysis Chinese Academy of SciencesBeijing 100190China

Due to the limited resources and budgets in many real-life projects, it is unaffordable to use full factorial experimental designs and thus fractional factorial(FF) designs are used instead. The aliasing of factorial effects is the price we pay for using FF designs and thus some significant effects cannot be estimated. Therefore, some additional observations(runs) are needed to break the linages among the factorial effects. Folding over the initial FF designs is one of the significant approaches for selecting the additional runs. This paper gives an in-depth look at fold-over techniques via the following four significant contributions. The first contribution is on discussing the adjusted switching levels foldover technique to overcome the limitation of the classical one. The second contribution is on presenting a comparison study among the widely used fold-over techniques to help experimenters to recommend a suitable fold-over technique for their experiments by answering the following two fundamental questions:Do these techniques dramatically lessen the confounding of the initial designs, and do the resulting combined designs(combining initial design with its fold-over) via these techniques have considerable difference from the optimality point of view considering the markedly different searching domains in each technique? The optimality criteria are the aberration, confounding, Hamming distance and uniformity. Many of these criteria are given in sequences(patterns) form, which are inconvenient and costly to represent and compare, especially when the designs have many factors. The third innovation is on developing a new criterion(dictionary cross-entropy loss) to simplify the existing criteria fromsequence to scalar. The new criterion leads to a more straightforward and easy comparison study. The final contribution is on establishing a general framework for the connections between initial designs and combined designs based on any fold-over technique.

关键词： Aberration confounding cross-entropy dictionary ordering fold-over Hamming distance loss function uniformity

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Hyperbaric Oxygen Treatment Improves Hearing Level via Attenuating TLR4/NF-κB Mediated Inflammation in Sudden Sensorineural Hearing Loss Patients

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Biomedical and Environmental Sciences 2020年第5期33卷 331-337页

作者： LIU Xue Hua LIANG fang JIA Xing yuan ZHAO lin ZHOU Yan YANG Jing Department of Hyperbaric Oxygen Medicine Beijing Chaoyang HospitalCapital Medical UniversityBeijing 100020China Institute of Basic Medicine Sciences Beijing Chaoyang HospitalCapital Medical UniversityBeijing 100020China Department of Hyperbaric Oxygen Medicine Beijing Pinggu HospitalBeijing 101200China

Objective Hyperbaric oxygen treatment(HBOT)has demonstrated efficacy in improving hearing levels of patients with idiopathic sudden sensorineural hearing loss(ISSHL);however,the underlying mechanisms are not well *** alleviates the inflammatory response,which is mediated by Toll-like receptor(TLR)4 and nuclear factor(NF)-κ*** this study we investigated whether HBOT attenuates inflammation in ISHHL patients via alteration of TLR4 and NF-κB *** ISHHL patients(n=120)and healthy control subjects(n=20)were enrolled in this *** were randomly divided into medicine group treated with medicine only(n=60)and HBO group receiving both HBOT and medicine(n=60).Audiometric testing was performed pre-and ***4,NF-кB,and TNF-αexpression in peripheral blood of ISSHL patients and healthy control subjects was assessed by ELISA before and after *** TLR4,NF-κB,and TNF-αlevels were upregulated in ISSHL patients relative to healthy control subjects;the levels were decreased following treatment and were lower in the HBO group than that in the medicine group post-treatment(Pling.

关键词： Hyperbaric oxygen treatment Sudden sensorineural hearing loss Toll-like receptor 4 Nuclear factor-kB

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Glow Discharge Plasma-Assisted Preparation of Nickel-Based Catalyst for Carbon Dioxide Reforming of Methane

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Chinese Journal of Chemical Physics 2008年第5期21卷 481-486页

作者： fang Guo Wei Chu Jun-qiang Xu lin Zhong Department of Chemical Engineering Sichuan University Chengdu 610065 China Department of Chemical and Biological Engineering Chongqing Institute of Technology Chongqing 400050 China

A plasma-assisted method was employed to prepare Ni/γ-All2O3 catalyst for carbon dioxide reforming of methane reaction. The novel catalyst possessed higher activity and better coke-suppression performance than those of the conventional calcination catalyst. To achieve the same CH4 conversion, the conventional catalyst needed higher reaction temperature, about 50 ℃ higher than that of the N2 plasma-treated catalyst. After the evaluation test, the deactivation rate of the novel catalyst was 1.7%, compared with 15.2% for the conventional catalyst. Different from the characterization results of the calcined catalyst, a smaller average pore diameter and a higher specific surface area were obtained for the plasma-treated catalyst. The variations of the reduction peak temperatures and areas indicated that the catalyst reducibility was promoted by plasma assistance. The dispersion of nickel was also remarkably improved, which was helpful for controlling the ensemble size of metal atoms on the catalyst surface. The modification effect of plasma- assisted preparation on the surface property of alumina supported catalyst was speculated to account for the concentration increase of absorbed CO2. An enhancement of CO2 adsorption was propitious to the inhibition of carbon formation. The coke amount deposited on plasma treated catalyst was much smaller than that on the conventional catalyst.

关键词： Nickel-based catalyst Plasma CO2 reforming of CH4 Coke Adsorption of CO2

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Rare mutation in MKRN3 in two twin sisters with central precocious puberty: Two case reports

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World Journal of Clinical Cases 2021年第32期9卷 10018-10023页

作者： Li-Qiong Jiang Yan-Qiong Zhou Ke yuan Jian-fang Zhu Yan-Lan fang Chun-lin Wang Department of Pediatrics The First Affiliated HospitalZhejiang University School of MedicineHangzhou 310003Zhejiang ProvinceChina

BACKGROUND Caused by premature activation of the hypothalamic-pituitary-gonadal axis,there is increasing incidence of central precocious puberty(CPP),especially in *** ring finger protein 3(MKRN3),a maternal imprinted gene with a highly conserved sequence,is the most common genetic etiology associated with *** 50 different mutations in MKRN3 have been found in *** SUMMARY This case report involves identical twin sisters presenting with premature thelarche at the age of 6 *** left hand bone age of both patients revealed advanced age(9 years).Pelvic B ultrasound indicated enlargement of the *** hormone(LH)releasing hormone testing confirmed *** sequencing detected the c.841C>T mutation in MKRN3,leading to a single base substitution,in the *** mutation was inherited from the father and paternal *** 3 mo of treatment with a gonadotropin-releasing hormone analog,levels of LH,follicle-stimulating hormone,and estradiol in the proband’s sister returned to normal *** Here,we report a rare mutation(c.841C>T)in MKRN3 in identical twin sisters with CPP.

关键词： Central precocious puberty MKRN3 Mutation Case report

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