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Alp7-Mtol and Alpl4 synergize to promote interphase microtubule regrowth from the nuclear envelope

Journal of Molecular Cell Biology 2019年第11期11卷 944-955页

作者： Wenyue Liu fan zheng Yucai Wang Chuanhai Fu Division of Molecular and Cell Biophysics Hefei National Science Center for Physical SciencesUniversity of Science and Technology of ChinaHefeiChina Chinese Academy of Sciences Center for Excellence in Molecular Cell Sciences School of Life SciencesUniversity of Science and Technology of ChinaHefeiChina Anhui Key Laboratory for Cellular Dynamics and Chemical Biology University of Science and Technology of ChinaHefeiChina School of Life Sciences University of Science and Technology of ChinaHefeiChina

Microtubules grow not only from the centrosome but also from various noncentrosomal microtubule-organizing centers(MTOCs),including the nuclear envelope(NE)and pre-existing *** evolutionarily conserved proteins Mtol/CDK5RAP2 and Alpl4/TOG/XMAP215 have been shown to be involved in promoting microtubule ***,it has remained elusive as to how the microtubule nucleation promoting factors are specified to various noncentrosomal MTOCs,particularly the NE,and how these proteins coordinate to organize microtubule ***,we demonstrate that in the fission yeast Schizosaccharomyces pombe,efficient interphase microtubule growth from the NE requires Alp7/TACC,Alpl4/TOG/XMAP215,and Mtol/*** absence of Alp7,A lp l4 t or Mtol compromises microtubule regrowth on the NE in cells undergoing microtubule *** further demonstrate that Alp7 and Mtol interdependently localize to the NE in cells without microtubules and that A lp l4 localizes to the NE in an Alp7 and Mtol-dependent *** Mtol to the NE in cells lacking Alp7 partially restores microtubule number and the efficiency of microtubule generation from the ***,our study delineates that Alp7,A lpl4,and Mtol work in concert to regulate interphase microtubule regrowth on the NE.

关键词： microtubule microtubule nucleation nuclear envelope microtubule-associated protein fission yeast

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Some notes on the spectral perturbations of the signless Laplacian of a graph

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Applied Mathematics(A Journal of Chinese Universities) 2014年第2期29卷 241-248页

作者： YU Gui-dong CAI Gai-xiang fan Yi-zheng School of Mathematics & Computation Sciences Anqing Normal University School of Mathematical Sciences Anhui University

Let G be a simple graph and let Q（G） be the signless Laplacian matrix of G. In this paper we obtain some results on the spectral perturbation of the matrix Q（G） under an edge addition or an edge contraction.

关键词： Graph signless Laplacian matrix spectral perturbation.

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CsTT8 regulates anthocyanin accumulation in blood orange through alternative splicing transcription

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Horticulture Research 2023年第10期10卷 215-226页

作者： Jianhui Wang Rui Xu Shuangping Qiu Weichun Wang fan zheng Department of Food Science and Engineering School of Food and Biological EngineeringChengdu UniversityChengdu 610106China Zhang Lan Honors College Chengdu UniversityChengdu 610106China

A homologous gene of basic-helix-loop-helix AtTT8 in Arabidopsis thaliana was identified in juice sac cells of pulp tissues from blood orange(Citrus sinensis cv‘Tarocco’),which was designated as CsTT8 in this ***,the mRNA levels of TT8 with the full-length open reading frame were significantly higher in‘Tarocco’than in mutant fruit lacking pigment in pulp or peel ***,an alternative splicing transcript,Δ15-TT8,with the fourth exon skipped,was also identified from transcripts different in length from that in‘Tarocco’.The mRNA levels ofΔ15-TT8 were higher in mutant fruit lacking pigment in pulp or peel tissues than in the wild ***,the TT8/Δ15-TT8 mRNA level ratio was found to be crucial for sufficient pigment in either pulp or peel ***8 from blood orange fruit demonstrated the capacity for nucleus localization and binding to other *** contrast,Δ15-TT8,lacking the fourth exon,lost its ability to interact with RUBY1 and to localize at the *** a dual luciferase reporter assay and transient overexpression in tobacco,we proved that two regulatory complexes formed by a functional TT8 with different MYB(v-myb avian myeloblastosis viral oncogene homolog)-type partners significantly promoted expression of an anthocyanin biosynthetic gene and a proton pumping gene,leading to anthocyanin and citrate *** findings suggest that TT8,rather than dysfunctionalΔ15-TT8,is possibly involved in modulating anthocyanin biosynthesis and its transport into vacuoles by proton ***,increased mRNA levels of the dysfunctional alternative splicing transcript may act as a negative feedback to downregulate TT8 expression and limit anthocyanin accumulation in blood oranges.

关键词： orange pumping transient

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Weak absorption test and defect analysis of optical coatings

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光学精密工程 2005年第4期13卷 430-434页

作者： HE Hong-bo LI Xia fan Shu-hai ZHAO Yuan-an SHAO Jian-da fan zheng-xiu Shanghai Institute of Optics and Fine Mechanics Chinese Academy of Sciences Shanghai 201800 China

Surface thermal lensing technique was developed into a high-sensitive apparatus for weak absorption test and defect analysis of optical coatings. A continuous-wave 1 064 nm Nd:YAG laser and a He-Ne laser were employed as pump source and probe source, respectively. Low noise photoelectrical components and a lock-in amplifier were used for photo-thermal deformation signal detection. In order to improve sensitivity, the apparatus configuration was optimized by choosing appropriate parameters, including pump beam spot size, chopper frequency, detection distance, waist radius and position of probe beam. Coating samples were mounted on a x-y stage. Different procedures, such as single spot, linear scan and 2-dimension area scan, could be performed manually or automatically. Various optical coatings were prepared by both electron beam evaporation and ion beam sputtering deposition. High sensitivity was obtained and low to 1×10 -7 weak absorption was tested in low-loss coating samples. For the sensitivity extreme of the system, 1×10 -8 absorption was reason out to be measured by surface thermal lensing technique. Very small standard deviation was achieved for the reproducibility evaluation. Moreover, a spatial resolution of 25 micron was proved according to the area scan which traced out the profile of photo-thermal defects inside optical coatings. The system was employed in the analyses of optical absorption, absorption uniformity and defect characterization, and revealed the relationship between laser-induced damage and absorption of optical coatings.

关键词：光学涂覆技术吸收能力稳定性激光技术

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Radiolarian transfer function for paleo-primary productivity in the South China Sea

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Chinese Science Bulletin 1999年第13期44卷 1232-1237页

作者： Muhong Chen Liangmin Huang Xia Tu fan zheng Chinese Acad Sci S China Sea Inst Oceanol Guangzhou 510301 Peoples R China

Basic data of radiolarian group and primary productivity were from 46 investigation stations of the South China Sea. The factor analysis of principal component and the least squares estimation in both linear regression and nonlinear estimation were applied to establishing the radiolarian transfer function for paleo-primary productivity in this sea area. The results show that the transfer function of nonlinear regression analysis for the radiolarian assemblage and primary productivity has much better parameters. Factors analysis has accumulating variance of 83.6% and communalities of more than 0.940347. Nonlinear regression analysis gives multiple correlation coefficient as 0.84888 and explained variance as 72.059%. The equation of transfer function is with an average estimated error of about 53.778 mg·C/m^2·d, which is 14.69% in average observed values. Calculation accuracy is 85.31% for the system of transfer function.

关键词： radiolarian paleo-primary productivity transfer function paleoceanography South China Sea.

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Synthesis of Cu_2O nanobelts via surfactant-assisted polyol method

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Chinese Chemical Letters 2011年第7期22卷 879-882页

作者： Xin Wen Huang Zong Jian Liu Yi fan zheng College of Biological and Environmental Engineering Zhejiang University of Technology Hangzhou 310014 China Resource & Environment Catalysis Institute College of Chemical Engineering and Materials Science Zhejiang University of Technology Hangzhou 310014 China

The synthesis of nanostructured Cu2O was conducted in a non-aqueous solution via polyol method at a temperature of 130 ℃. It was found that the formation of Cu2O nanobelts was heavily dependent on the presence of surfactant cetyltrimethylammonium bromide （CTAB）. Cu2O nanobelts with 100400 nm in width and several micrometers in length were only observed when CTAB was used, whereas in other cases where CTAB was absent or CTAB was replaced with polyvinylpyrrolidone （PVP K-30）, only spherical or cube-like particles were formed. The formation mechanism of Cu2O nanobelts was discussed.

关键词： Cuprous oxide Nanobelts Surfactant Polyol method

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Isotope distribution of iridium in the interaction of 47MeV/nucleon ^(12)C with ^(197)Au

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Nuclear Science and Techniques 2004年第6期15卷 344-347页

作者： YANGWei-fan ZHAOZhi-zheng XUYan-Bing YUANShuang-Gui MOUWan-Tong InstituteofModernPhysics theChineseAcademyofSciencesLanzhou730000

Thick gold targets were bombarded with 47 MeV/nucleon C ions and the radioactive isotopes of iridium 12 and platinum were produced through the multi-nucleon transfer reactions. The production cross-sections of iridium isotopes in the reactions have been determined by a combination of radiochemical separation and off-line γ-ray spec- troscopy technique. The obtained Ir isotope distribution was compared with those obtained in other experiment as well as calculated by the statistical model.

关键词：铱同位素分布多核子变换反应中子丰度重离子

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Austenite grain growth of medium-carbon alloy steel with aluminum additions during heating process

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International Journal of Minerals,Metallurgy and Materials 2019年第3期26卷 282-290页

作者： Zi-yi Liu Yan-ping Bao Min Wang Xin Li fan-zheng Zeng State key Laboratory of Advanced Metallurgy University of Science and Technology Beijing Xiangtan Iron & Steel Co. Ltd.of Hunan Valin

In this study, the effects of heating temperature(850–1100°C) and holding time(30–150 min) on the grain growth behavior of austenite in medium-carbon alloy steel were investigated by conducting experiments. The abnormal grain growth and mixed grain structure phenomenon are explained using an equilibrium precipitation phase diagram calculated by Thermo-Calc software package. The Al N particles were observed by field-emission scanning electron microscopy(FESEM), and the amount of AlN precipitations was detected by electron probe microanalysis(EPMA). Based on the research results, it was found that the average grain size of austenite in the test steel increased continuously with the increase of temperature and holding time. Furthermore, the abnormal growth of austenite occurred in the test steel at 950°C, and the heating temperature affected the austenite grain size more significantly. In addition, the decline in the amount of AlN second-phase particle in the test steel, which weakened the "pinning" effect on austenite grain boundaries, resulted in abnormal growth and the development of mixed austenite grain structures. The prediction model for describing the austenite grain growth of medium-carbon alloy steel during heating was established by regression analysis of the experimental data, and the model was verified to be highly accurate.

关键词： alloy steel austenite grain AlN growth model

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Influence of Profile of Blast Furnace on Motion and Stress of Burden by 3D-DEM

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Journal of Iron and Steel Research International 2011年第11期18卷 1-6,11页

作者： ZHANG Jian-liang CHEN Yong-xing fan zheng-yun HU zheng-wen YANG Tian-jun Tatsuro Ariyama School of Metallurgical and Ecological Engineering University of Science and Technology Beijing Beijing 100083 China Shougang Research Institute of Technology Beijing 100043 China Institute of Multidisciplinary Research for Advanced Materials Tohoku University Sendai 980-8577 Japan

The objective of the present investigation is to analyze the influence of profile of blast furnace on the burden motion and stress field through 3D-DEM （three-dimensional discrete element method）. It is clarified that the decrease of shaft angle speeds up the velocity of burden descending and decreases normal stress between particles or particle and wall. This change is good for the smooth operation of blast furnace. However, ore and coke would be mixed for the too small shaft angle （75~） , which would influence the permeability in blast furnace. Thus, the appro- priate shaft angle is around 80°. Decrease of bosh angle prevents the burden descending motion and increases normal stress between particles and bosh wall. Meanwhile, maximum normal stress acting on the wall moves from belly wall to bosh wall in the case of 68° bosh angle, which accelerates abrasion of refractory in bosh by friction force between particles and wall. Although burden descends smoothly in the case of 88° bosh angle, room is not enough for the as- cending heated gas flow. Thus, the appropriate bosh angle is about 78°.

关键词： blast furnace profile solid motion stress discrete element method

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Robot Intelligence for Real World Applications

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Chinese Journal of Electronics 2018年第3期27卷 446-458页

作者： LIU Yunhui zheng fan GUO Ruibin WANG Jiangliu NIE Qiang WANG Xin WANG Zerui T Stone Robotics Institute The Chinese University of Hong Kong National University of Defense Technology

This paper presents a brief review on recent work on machine intelligence for real-world applications of *** act in a real world environment,a robot should possess a broad sense of intelligence including speech,perception,reasoning,action,*** this paper,we particularly deal with the intelligence involving action or body *** intelligence related to robot action/motion can be classified into two categories:manipulation intelligence and mobility *** manipulation intelligence means the skill/intelligence of reliably manipulating objects according to tasks and the mobility intelligence corresponds to the ability of autonomously moving,or flying,and or jumping in a natural ***-robot interaction is another important topic for real-world *** addition to reviewing the ma jor approaches,this paper also gives an overview on our efforts in these important topics.

关键词： Machine intelligence Simultaneous localization and mapping(SLAM) Medical robots Humanrobot interaction

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