检索结果-南通市图书馆

Science China Materials 2019年第4期62卷 566-576页

作者： Zhengong Meng Cheuk-Lam Ho Hon-fai wong Zhen-Qiang Yu Nianyong Zhu Guijun Li Chi-Wah Leung Wai-Yeung wong College of Chemistry and Environmental Engineering Shenzhen University Institute of Molecular Functional Materials and Department of Chemistry Hong Kong Baptist University HKBU Institute of Research and Continuing Education Department of Applied Biology and Chemical Technology The Hong Kong Polytechnic University Department of Applied Physics The Hong Kong Polytechnic University

Patterning of L10 FePt nanoparticles(NPs) with high coercivity offers a promising route to develop bit-patterned media(BPM) for the next generation magnetic data recording system, but the synthesis of monodisperse FePt NPs and mass production of their nanopatterns has been a longstanding challenge. Here, highly efficient nanoimprint lithography was applied for large-scale universal patterning, which was achieved by imprinting the solution of a single-source bimetallic precursor. The rigid coplanar metallic cores and the surrounding flexible tails in the bimetallic complex permit the spontaneous molecular arrangements to form the highly ordered negative morphology replicated from the soft ***-situ pyrolysis study was then investigated by one-pot pyrolysis of the precursor under an Ar/H2 atmosphere, and the resultant NPs were fully characterized to identify the phase,morphology and magnetic properties. Finally, highly-ordered patterns on certain substrates were preserved perfectly after pyrolysis and could be potentially utilized in magnetic data recording media.

关键词： FePt nanoparticles single-source bimetallic precursors one-pot pyrolysis lithographic patterning magnetic data recording media

Impact of disease severity on gastric residual volume in critical patients

在线全文

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2011年第15期17卷 2007-2012页

作者： Chien-Wei Hsu Shu-Fen Sun David Lin Lee Shoa-Lin Lin Kam-fai wong Hsiu-Hua Huang Hung-Ju Li Department of Medicine School of Medicine National YangMing University Taipei Taiwan China Intensive Care Unit Department of Medicine Kaohsiung Veterans General Hospital Kaohsiung City 813 Taiwan China Department of Physical Medicine and Rehabilitation Kaohsiung Veterans General Hospital Kaohsiung City 813 Taiwan China Institute of Statistics National University of Kaohsiung Kaohsiung City 813 Taiwan China Department of Nutrition Kaohsiung Veterans General Hospital Kaohsiung City 813 Taiwan China

AIM: To investigate whether illness severity has an impact on gastric residual volume (GRV) in medical critically ill patients. METHODS: Medical intensive care unit (ICU) patients requiring nasogastric feeding were enrolled. Sequential Organ failure Assessment (SOFA) score was assessed immediately preceding the start of the study. Acute Physiology and Chronic Health Evaluation (APACHE) Ⅱ scores were recorded on the first, fourth, seventh, and fourteenth day of the study period. GRV was measured every 4 h during enteral feeding. The relationship be-tween mean daily GRV and SOFA scores and the correlation between mean daily GRV and mean APACHE Ⅱ score of all patients were evaluated and compared. RESULTS: Of the 61 patients, 43 patients were survivors and 18 patients were non-survivors. The mean daily GRV increased as SOFA scores increased (P < 0.001, analysis of variance). Mean APACHE Ⅱ scores of all patients correlated with mean daily GRV (P = 0.011, Pearson correlation) during the study period. Patients with decreasing GRV in the first 2 d had better survival than patients without decreasing GRV (P = 0.017, log rank test). CONCLUSION: GRV is higher in more severely ill medical ICU patients. Patients with decreasing GRV had lower ICU mortality than patients without decreasing GRV.

关键词： Critical care Outcome Residual volume Severity of illness index Tube feeding

Purification and identification of novel cytotoxic oligopeptides from soft coral Sarcophyton glaucum

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2019年第1期20卷 59-70页

作者： Yixian QUAH Nor Ismaliza MOHD ISMAIL Jillian Lean Sim OOI Yang Amri AFFENDI Fazilah ABD MANAN Lai-Kuan TEH fai-Chu wong Tsun-Thai CHAI Department of Chemical Science Faculty of Science Universiti Tunku Abdul Rahman Department of Biological Science Faculty of Science Universiti Tunku Abdul Rahman Centre for Biodiversity Research Universiti Tunku Abdul Rahman Department of Geography Faculty of Arts and Social Sciences University of Malaya Institute of Biological Sciences Faculty of Science University of Malaya Institute of Ocean and Earth Sciences University of Malaya Department of Biosciences Faculty of Science Universiti Teknologi Malaysia Department of Biomedical Science Faculty of Science Universiti Tunku Abdul Rahman

Globally, peptide-based anticancer therapies have drawn much attention. Marine organisms are a reservoir of anticancer peptides that await discovery. In this study, we aimed to identify cytotoxic oligopeptides from Sarcophyton glaucum. Peptides were purified from among the S. glaucum hydrolysates produced by alcalase, chymotrypsin, papain, and trypsin, guided by a methylthiazolyldiphenyl-tetrazolium bromide(MTT) assay on the human cervical cancer(HeLa) cell line for cytotoxicity evaluation. Purification techniques adopted were membrane ultrafiltration, gel filtration chromatography, solid phase extraction(SPE), and reversed-phase high-performance liquid chromatography(RP-HPLC). Purified peptides were identified by de novo peptide sequencing. From papain hydrolysate, three peptide sequences were identified: AGAPGG, AERQ, and RDTQ(428.45, 502.53, and 518.53 Da, respectively). Peptides synthesized from these sequences exhibited cytotoxicity on HeLa cells with median effect concentration(EC50) values of 8.6, 4.9, and 5.6 mmol/L, respectively, up to 5.8-fold stronger than the anticancer drug 5-fluorouracil. When tested at their respective EC50, AGAPGG, AERQ, and RDTQ showed only 16%, 25%, and 11% cytotoxicity to non-cancerous Hek293 cells, respectively. In conclusion, AERQ, AGAPGG, and RDTQ are promising candidates for future development as peptide-based anticancer drugs.

关键词： Anticancer therapy Bioactive peptide Cytotoxicity HeLa cells Sarcophyton glaucum Soft coral

在线全文

学校读者我要写书评

暂无评论

模板驱动的神经机器翻译

计算机学报 2019年第3期42卷 566-581页

作者：李强黄辉周沁韩雅倩肖桐朱靖波东北大学自然语言处理实验室沈阳110000 澳门大学自然语言处理与中葡机器翻译实验室澳门999078 沈阳雅译网络技术有限公司沈阳110000

由于神经机器翻译模型简单、通用和有效,神经机器翻译模型已成为目前最受关注的机器翻译模型.在神经机器翻译模型中,通过引入词汇翻译表和短语翻译表可以提高翻译质量.然而,对于已经存在的人工整理的翻译模板或者启发式算法生成的翻译模... 详细信息

由于神经机器翻译模型简单、通用和有效,神经机器翻译模型已成为目前最受关注的机器翻译模型.在神经机器翻译模型中,通过引入词汇翻译表和短语翻译表可以提高翻译质量.然而,对于已经存在的人工整理的翻译模板或者启发式算法生成的翻译模板,目前已有的神经机器翻译框架不存在有效的方法对这些翻译模板进行建模.该文研究的主要内容是将翻译模板内嵌到端到端的神经机器翻译模型中.为此,我们提出了模板驱动的神经机器翻译模型,该模型通过使用额外的模板编码器对翻译模板进行端到端建模,通过使用知识门阀和注意力门阀动态地控制解码过程中不同来源的知识对当前解码词汇的贡献度的大小.知识门阀的主要作用是对源语言句子和翻译模板的信息进行有效的表示,从而更好地对解码器进行初始化.注意力门阀是一个基于时序的门阀,可以动态地控制当前翻译词汇接收源语言句子或者翻译模板信息的多少.最终实验结果表明,该文提出的方法对模板进行了有效的建模,20%词汇标准模板在汉英和英汉翻译任务上的翻译正确率分别高达93.6%和95.1%.与基线翻译系统相比,在汉英和英汉翻译任务上使用含有20%词汇的标准模板时,翻译性能可以增长4.2～7.2个BLEU值.当翻译模板中的真实词汇增加时,翻译质量得到进一步提升.

关键词：人工智能自然语言处理神经机器翻译翻译模板门阀

在线全文

学校读者我要写书评

暂无评论

中文网络聊天语言的奇异性与动态性研究

中文信息学报 2007年第3期21卷 83-91页

作者：夏云庆黄锦辉张普清华大学信息技术研究院北京100084 香港中文大学系统工程系北京语言大学网络教育学院北京100083

随着互联网走入社会生活,网络聊天逐渐成为一种新的沟通渠道,网络聊天语言便应运而生。这类语言的日益丰富,给语言信息处理带来了新的挑战。研究发现,困难主要来自网络聊天语言的奇异性和动态性。本文借助真实网络聊天语言文本,对网络... 详细信息

随着互联网走入社会生活,网络聊天逐渐成为一种新的沟通渠道,网络聊天语言便应运而生。这类语言的日益丰富,给语言信息处理带来了新的挑战。研究发现,困难主要来自网络聊天语言的奇异性和动态性。本文借助真实网络聊天语言文本,对网络聊天语言的奇异性和动态性进行详细分析和归纳,并设计了面向解决奇异性和动态性问题的网络聊天语言文本识别与转换方法。我们先以网络聊天语言语料库为基础建立网络聊天语言模型和语言转换模型,通过信源?信道模型实现网络聊天语言向标准语言的转换。但该方法过于依赖网络聊天语言语料库,虽然能较好解决奇异性问题,但不能处理动态性问题。因此,我们进而以标准汉语语料库为基础建立文字语音映射模型,对信源?信道模型进行改进,最终有效解决了网络聊天语言的动态性问题。

关键词：计算机应用中文信息处理网络聊天语言奇异性动态性语言信息处理

Edible Bird’s Nest, an Asian Health Food Supplement, Possesses Skin Lightening Activities: Identification of N-Acetylneuraminic Acid as Active Ingredient

在线全文

学校读者我要写书评

暂无评论

Journal of Cosmetics, Dermatological Sciences and Applications 2015年第4期5卷 262-274页

作者： Gallant Kar Lun Chan Zack Chun fai wong Kelly Yin Ching Lam Lily Kwan Wai Cheng Laura Minglu Zhang Huangquan Lin Tina Tingxia Dong Karl Wah Keung Tsim Division of Life Science and Center for Chinese Medicine R&D The Hong Kong University of Science and Technology Kowloon Hong Kong China

Edible bird’s nest (EBN;Yan Wo), or cubilose, is originated from the salivary secretion of Aerodramus fuciphagus. In Asia, EBN is famous for its unproven skin lightening function. Here, we aim to reveal the active ingredients of EBN responsible for skin lightening function. Three major fractions were isolated from EBN water extract by chromatography using LC-MS/MS, bioactivities of these isolated fractions were analyzed by assays of tyrosinase, melanocytes and 3D human skin model, from which, N-acetylneuraminic acid (NANA), the second isolated fraction showed an inhibition effect on tyrosinase activity in a dose-dependent manner. The IC50 of tyrosinase originated from mushroom and human was 16.93 mM and 0.10 mM respectively. Furthermore, only EBN with higher content of NANA (e.g. White and Red EBN), but not EBN with less NANA (e.g. Grass EBN), showed promising skin lightening function. This is the first report to reveal NANA being an active ingredient of EBN on skin lightening function.

关键词： Cubilose N-Acetylneuraminic Acid Chemical Analysis Skin Lightening Human Skin Model

茴香种子蛋白质水解物和肽的抗氧化和蛋白质保护潜力（英文）

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

现代食品科技 2019年第9期35卷 22-29,21页

作者：摩哈娜达斯·莎美拉蔡俊泰黄怀祖拉曼大学理学院马来西亚金宝31900 拉曼大学生物多样性研究中心马来西亚金宝31900

茴香种子是一种可食用的香料,经常用于增进各种烹饪的香气和味道,并且因其药用价值而在不同文化中被珍藏。之前的研究报告提及了与茴香种子相关的各种生物活性。然而,对于来自茴香种子的水解蛋白质知之甚少。在本文中,作者从茴香种子中... 详细信息

茴香种子是一种可食用的香料,经常用于增进各种烹饪的香气和味道,并且因其药用价值而在不同文化中被珍藏。之前的研究报告提及了与茴香种子相关的各种生物活性。然而,对于来自茴香种子的水解蛋白质知之甚少。在本文中,作者从茴香种子中分离出总蛋白质,然后使用四种不同的蛋白酶进行酶水解。本文中也报道了水解度,pH和温度曲线。在ABTS自由基清除测定的指引下,作者使用离心过滤,C18反相和强阳离子交换(SCX)来进一步分离碱性蛋白酶消化的蛋白质水解物。与粗水解物相比,纯化水解物的自由基清除潜力增强了4.5倍。通过肽测序进一步分析纯化的SCX级分,本文也鉴定出两个肽序列,其范围为6~8个氨基酸。紧接着,文中也报道了使用白蛋白变性抑制试验来测试茴香肽的蛋白质保护潜力,其中一种茴香肽(EDVDFR)更被测出与纯谷胱甘肽(GSH)相当的EC50值。总要言之,茴香种子衍生的蛋白质水解物和茴香肽有潜力被进一步开发成促进健康的补充剂或作为食品添加剂用于保存富含蛋白质的食物。

关键词：抗氧化肽酶水解茴香籽蛋白质保护 pH和热稳定性肠稳定性

Identification of a novel salt tolerance gene in wild soybean by whole-genome sequencing

在线全文

学校读者我要写书评

暂无评论

Identification of a novel salt tolerance gene in wild soybea...

决明子蛋白水解产物的抗氧化活性：热和胃肠稳定性，肽鉴定和计算机分析（英文）

第十四届全国中药和天然药物学术研讨会

作者： Xinpeng Qi Man-Wah Li Min Xie Xin Liu Meng Ni Guihua Shao Chi Song Aldrin Kay-Yuen Yim Ye Tao Fuk-Ling wong Sachiko Isobe Chi-fai wong Kwong-Sen wong Chunyan Xu Chunqing Li Ying Wang Rui Guan Fengming Sun Guangyi Fan Zhixia Xiao Feng Zhou Tsui-Hung Phang Xuan Liu Suk-Wah Tong Ting-Fung Chan Siu-Ming Yiu Satoshi Tabata Jian Wang Xun Xu Hon-Ming Lam School of Life Sciences and Center for Soybean Research of the State Key Laboratory of Agrobiotechnology The Chinese University of Hong Kong BGI-Shenzhen Institute of Crop Sciences The Chinese Academy of Agricultural Sciences Kazusa DNA Research Institute Department of Computer Science The University of Hong Kong

＜正＞Using a whole-genome-sequencing approach to explore germplasm resources can serve as an important strategy for crop improvement,especially in investigating wild accessions that may contain useful genetic resources that have been lost during the domestication *** we sequence and assemble a draft genome of wild soybean and construct a

关键词：

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

现代食品科技 2019年第9期35卷 38-48页

作者：蔡俊泰徐芷茵徐国强李仁杰阿都马南·法希拉黄怀祖拉曼大学理学院马来西亚金宝31900 拉曼大学生物多样性研究中心马来西亚金宝31900 中国医科大学营养系台湾台中40402 马来西亚理工大学生物科学系马来西亚新山81310

决明子乃决明(Cassia obtusifolia L.)的干燥成熟种子,被中医界用于治疗各种疾病。本研究探讨决明子水解产物的抗氧化活性和稳定性,并鉴定其中的短肽。决明子蛋白经过碱性蛋白酶水解2~6 h,以超滤膜技术产生<3000 u肽组分。UF 2 h(2 h水... 详细信息

决明子乃决明(Cassia obtusifolia L.)的干燥成熟种子,被中医界用于治疗各种疾病。本研究探讨决明子水解产物的抗氧化活性和稳定性,并鉴定其中的短肽。决明子蛋白经过碱性蛋白酶水解2~6 h,以超滤膜技术产生<3000 u肽组分。UF 2 h(2 h水解产物分离出来的<3000 u组分)表现出最强的ABTS^·+清除能力(EC50=229μg/mL)和铁螯合能力(EC50=89μg/mL)。经过热处理和模拟胃肠消化后,UF 2 h的ABTS^·+清除能力都得以保留。UF 2 h仅在模拟胃肠消化胃后保留铁螯合活性。试验通过使用固相萃取,凝胶色谱和高效液相色谱对UF 2 h进行分离纯化。通过质谱分析,鉴定了四个肽:PMPVR(599.29 u),FETLPF(752.34 u),KMRDNL(775.37 u)和LDESKRF(893.50 u)。计算机分析预测,在模拟胃肠消化前,四个肽皆无毒无过敏性。胃肠消化后,四个肽释放的片段仍无毒,仅PMPVR和KMRDNL释放的肽片段无过敏性的。UF 2 h及其活性肽可作为抗氧化剂,用于开发功能性食品和营养保健品。

关键词：致敏性抗氧化肽决明胃肠稳定性热稳定性

Anti-cancer activities of S-allylmercaptocysteine from aged garlic

在线全文

学校读者我要写书评

暂无评论

Chinese Journal of Natural Medicines 2019年第1期17卷 43-49页

作者： LV Yi SO Kwok-fai wong Nai-Kei XIAO Jia Laboratory of Neuroendocrinology School of Biological SciencesFujian Normal University National Key Disciplines for Infectious Diseases Shenzhen Third People's HospitalThe Second Hospital Affiliated to Southern University of Science and Technology School of Biomedical Sciences Li Ka Shing Faculty of MedicineThe University of Hong Kong

While most types of malignancies remain recalcitrant to treatment, application of natural products or their analogs in daily life has offered some hopes as an effective prophylaxis against cancer onset and progression in the past decades. Emerging evidence supports a link between garlic consumption and decreased cancer incidence. Notably, aged garlic extract(AGE) exhibits stronger anti-cancer activities than that of fresh garlic, by virtue of enrichment of several AGE-specific organosulfur compounds, including S-allylmercaptocysteine(SAMC). In this review, we summarize the up-to-date mechanistic pathways associated with the anti-proliferative, anti-metastatic and pro-apoptotic effects of SAMC in various cancer models. Based upon the proven safety and improved understanding on its anti-neoplastic properties, SAMC has gained recognition as a promising daily food supplement for cancer prevention or management.

关键词： Aged garlic S-allylmercaptocysteine Cancer Molecular pathway Reactive oxygen species