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Selection and characterisation of lactic acid bacteria isolated from different origins for ensiling Robinia pseudoacacia and Morus alba ***

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Journal of Integrative Agriculture 2016年第10期15卷 2353-2362页

作者： NI Kui-kui YANG Hui-xiao HUA Wei WANG Yan-ping PANG Hui-li Henan Provincial Key Laboratory of Ion Beam bio-Engineering Zhengzhou University Zhengzhou 450051 P.R. China Henan Sanoterre bio-Tec Company Zhengzhou 450000 P.R.China

The objective of this study was to isolate lactic acid bacteria（LAB） strains from different origins and to select the best strains for ensiling Robinia pseudoacacia（RB） and Morus alba L.（MB）leaves. The LAB strains were inoculated into the extracted liquid obtained from RB and MB leaves to evaluate the fermentation products. 11 LAB strains were selected for further experiments based on the highest products of lactic or acetic acid, including 1 strain of Weissella confusa, 2 of Lactobacillus reuteri and 8 of Lactobacillus *** API 50 CH fermentation experiment indicated that all of the selected 11 LAB strains utilised most of the carbohydrates. All the strains grew at temperatures between 10 and 45℃ and at a p H of 3.5 to 4.5; however, L. reuteri f7 and f8 tolerated a p H as low as 3.0. All 11 LAB strains showed antibacterial activity against Listeria monocytogens, Escherichia coil, Salmonella sp. and Acetobacter pasteurianus; however, after excluding the effect of organic acids, only f7 and f8 still exhibited antibacterial activity. The present study indicated that the selected 11 LAB strains could be used to prepare silages of RB and MB leaves, especially L. reuteri f7 and f8.

关键词： lactic acid bacteria Morus alba L Robinia pseudoacacia silage

Synthesis,characterization and photodynamic activity of ethanolamino-substituted hypocrellin B(EAHB2)

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生物物理学报 2009年第S1期25卷 441-442页

作者： Zhixiang Zhou, Lina Zhang,Ping Ai,Hongtao Ma College of Life Science and bio-engineering, Beijing University of Technology, Beijing, 100124, China

The limitations of currently used clinical phototherapeutic agent, Photofrin, have prompted a search for more ideal photodynamic sensitizer. The hypocrellins, have been paid the

关键词： Photodynamic therapy(PDT) ethanolamino-substituted hypocrellin B(EAHB2) MGC-803 cell MCf-7 cells cell cycle arrest apoptosis

Second-growth and small forest clearings have little effect on the temporal activity patterns of Amazonian phyllostomid bats

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Current Zoology 2020年第2期66卷 145-153页

作者： Ricardo ROCHA Adria Lopez-Baucells f.bio Z.farneda Diogo f.ferreira Ines SiLVA Marta AcAcio Jorge M.Palmeirim Christoph f.J.M eyer Centre for Ecology Evolution and Environmental ChangesFaculty of SciencesUniversity of LisbonLisbon1749-016Portugal biological Dynamics of forest fragments Project National Institute for Amazonian Research and Smithsonian Tropical Research InstituteManaus69011-970Brazil Conservation Science Group University of CambridgeCambridgeCB23EJUK Granollers Museum of Natural Sciences Granollers08402Spain Department of Ecology Federal University of Rio de JaneiroRio de Janeiro21941-902Brazil CIBI0-lnBI0 Research Centre in Biodiversity and Genetic ResourcesUniversity of PortoVairao4485-661Portugal Conservation Ecology Program School of Bioresources and TechnologyKing Mongkufs University of Technology ThonburiBangkok10150Thailand School of Environmental Sciences University of East AngliaNorwichNR47TJUKand'Ecosystems Environment Research Centre(EERC) School of ScienceEngineering and EnvironmentUniversity of SalfordSalfordM54WTUK

Secondary forests and human-made forest gaps are conspicuous features of tropical ***,behavioral responses to these aspects of anthropogenically modified forests remain poorly ***,we analyze the effects of small human-made clearings and secondary forests on tropical bats by examining the guild-and species-level activity patterns of phyllostomids sampled in the Central Amazon,***,we contrast the temporal activity patterns and degree of temporal overlap of 6 frugivorous and 4 gleaning animalivorous species in old-growth forest and second-growth forest and of 4 frugivores in old-growth forest and forest *** activity patterns of frugivores and gleaning animalivores did not change between old-growth forest and second-growth,nor did the activity patterns of frugivores between old-growth forest and ***,at the species level,we detected significant differences for Artibeus obscurus(old-growth forest ***-growth)and ***(old-growth forest ***).The degree of temporal overlap was greater than random in all sampled ***,for frugivorous species,the degree of temporal overlap was similar between old-growth forest and second-growth;whereas for gleaning animalivores,it was lower in second-growth than in old-growth *** the contrary,forest clearings were characterized by increased temporal overlap between *** in activity patterns and temporal overlap may result from differential foraging opportunities and dissimilar predation ***,our analyses suggest that activity patterns of bats in second-growth and small forest clearings,2 of the most prominent habitats in humanized tropical landscapes,varies little from the activity patterns in old-growth forest.

关键词： Amazon behavior forest gaps niche partitioning secondary forest temporal overlap

Automatic Airway Deletion in Pulmonary Segmentation

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Journal of Shanghai Jiaotong university(Science) 2005年第2期10卷 190-192页

作者：王平庄天戈 Dept. of.bio-medical Eng. Shanghai Jiaotong Univ. Shanghai 200030 China

A method of removing the airway from pulmonary segmentation image was proposed. This method firstly segments the image into several separate regions based on the optimum threshold and morphological operator, and then each region is labeled and noted with its mean grayscale. Therefore, most of the non-lung regions can be removed according to the tissue’s Hounsfield units (HU) and the imaging modality. finally, the airway region is recognized and deleted automatically through using the priori information of its HU and size. This proposed method is tested using several clinical images, yielding satisfying results.

关键词： computerized tomography image segmentation optimal threshold

Identification and functional analysis of a novel box C/D snoRNA from Schizosaccharomyces pombe

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Chinese Science Bulletin 2004年第18期49卷 1929-1936页

作者： LUO Yuping, ZHUO Hui, LI Siguang & QU Lianghu Key Laboratory of Gene Engineering of the Ministry of Education, bio-technology Research Center Zhongshan University, Guangzhou 510275, China Correspondence should be addressed to Qu Lianghu (e-mil: lsbrc04@***) Key Laboratory of Gene Engineering of the Ministry of Education Biotechnology Research Center Zhongshan University Guangzhou 510275 China

By constructing and screening the Schizosac-charomyces pombe nuclear cDNA library, a novel small nu-cleolar RNAs (snoRNA) was identified. The novel snoRNAdisplays structural features typical of C/D box snoRNA fam-ily and possesses a 10-nt-long rRNA antisense element whichis predicted to guide the 2’-O-methylation of the fission yeast 25S rRNA at G940. As expected, the rRNA ribose-methyla-tion site predicted by the novel snoRNA was preciselymapped by a deoxynucleoside triphosphate concentration-dependent primer extension assay. The comparison of func-tional element of guide snoRNAs among eukaryotes reveals that the novel snoRNA is a partial counterpart of the bud-ding yeast snR60 and was termed snR60-II. snR60-II genenested in the intron of a non-coding RNA gene with an un-known function, which is the first example of a yeastsnoRNA encoded in an intron of a non-coding RNA ***, a number of yeast snR60 homologues were also identified from other fungi and fly. Our results reveal thatsnR60 exhibits diverse genomic organization in eukaryotes, implying the high mobility of snR60 gene in the course ofevolution.

关键词：功能分析 C/DsnoRNA 人体酵母菌探针 rRNA 甲基化核酸分析

Physiological responses of osteoblasts to cyclic stretching and the change of intracellular calcium concentration

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Chinese Science Bulletin 2003年第4期48卷 346-350页

作者： TANG Liling, WANG Yuanliang, GU Li, CAI Shaoxi & SU Aihua bio-engineering College, Chongqing University, Chongqing 400044, China Correspondence should be addressed to Tang Liling bio-engineering College Chongqing University Chongqing 400044 China

The development of bone tissues is regulated by mechanical stimulation. Cyclic stretching was applied to the osteoblasts that were delivered from rat calvarie. The results showed that stretching at 500 me increased the cell prolifera-tion while loading at 1000 me and 1500 me inhabited cell growth. Loading also increased the adhesive force between cells and substrate as well as spreading areas of osteobalsts. furthermore, the fluorescence probe fluo-3/AM was used to investigate the effect of stretching stimulation on the intra-cellular calcium concentration of osteoblasts. The intracellu-lar calcium concentration of osteoblasts that were stretched at 500 me for 5 min was 92.9% higher than the control. After being treated with the panax ontoginseng saponins, the stretched osteoblasts still expressed 28.6% higher intracellu-lar calcium concentration than that of the control, which proved that both the influx of extracellular calcium and the release of intracellular calcium store were involved in the increase of intracellular calcium concentration when os-teoblasts responded to the cyclic stretching. And the influx of extracellular calcium through transmembrance channel played a main role.

关键词：成骨细胞定期拉伸物理性刺激细胞内钙浓度化学刺激生理反应骨质密度

Phosphorus forms and distribution in the sediments of Poyang Lake,China

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International Journal of Sediment Research 2011年第2期26卷 230-238页

作者： XIANG Su-lin and ZHOU Wen-bin 1 Lecturer,Key Lab of Poyang Lake Ecology and bio-resource Utilization,Ministry of Education,Nanchang University,Nanchang 330047,China and Department of Environmental Engineering,East China JiaoTong University,Nanchang 330013,China, 2 Prof,Key Lab of Poyang Lake Ecology and bio-resource Utilization,Ministry of Education,Nanchang University,Nanchang 330047,China [a]Lecturer Key Lab of Poyang Lake Ecology and Bio-resource Utilization Ministry of Education Nanchang University Nanchang 330047 China and Department of Environmental Engineering East China JiaoTong University Nanchang 330013 China [b]Prof. Key Lab of Poyang Lake Ecology and Bio-resource Utilization Ministry of Education Nanchang University Nanchang 330047 China

The forms and distribution of phosphorus in the sediments of Poyang Lake were studied using chemical extraction methods to understand the potential release and adsorption of *** results showed that phosphorus in the sediments mainly consisted of inorganic phosphorous species, including iron bound phosphorus,calcium bound phosphorus,aluminium bound phosphorus and dissolved phosphorus,as well as organic phosphorous *** spatial distribution of phosphorus species in surface sediments showed a decreasing trend from the outlet to the entrance of Poyang *** content of total phosphorus in the surface sediments ranged from 688.29 to 825.36 mg/kg, and inorganic phosphorous was the major phosphorus *** bound phosphorus was the dominant form of inorganic phosphorous,accounting for greater than 40%of the total phosphorus, while calcium bound phosphorus and aluminium bound phosphorus accounted for more than 20%, and dissolved phosphorus accounted for less than 5%.The content of organic phosphorus in surface sediments was lower,accounting for less than 15%.Total phosphorus was positively correlated with iron bound phosphorus,aluminium bound phosphorus,calcium bound phosphorus and organic phosphorous,while organic phosphorous was positively correlated with iron bound phosphorus and aluminium bound phosphorus,but only weakly related to calcium bound *** all six sites, the contents of total phosphorus and phosphorus forms in the core sediments obviously decreased with depth,while they were significantly enriched in the surface *** total phosphorus and phosphorus forms contents were significantly higher in the upper layers and had a tendency to decrease toward the bottom of the core,regardless of the phosphorus *** addition,the average contents of total phosphorus and various phosphorus forms decreased rapidly with sediment depth from 0-20 cm,and then decreased slightly from 20-30 cm.

关键词： Poyang Lake Phosphorus forms Distribution characteristics Sediment

Chlamydoconidium-producing Trichophyton tonsurans: Atypical morphological features of strains causing tinea capitis in Ceará, Brazil

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Asian Pacific Journal of Tropical Medicine 2019年第8期12卷 380-384页

作者： Raimunda Samia Nogueira Brilhante Germana Costa Paixao Jonathas Sales de Oliveira Vandbergue Santos Pereira Marcos f.bio Gadelha Rocha Reginaldo Goncalves de Lima-Neto Débora de Souza Collares Maia Castelo-Branco Rossana de Aguiar Cordeiro JoséJúlio Costa Sidrim Specialized Medical Mycology Center Postgraduate Program in Medical MicrobiologyFederal University of CearáRua Nunes de Melo1315FortalezaBrazil Postgraduate Program in Veterinary Sciences State University of CearáAv.Dr.Silas Munguba1700FortalezaBrazil Department of Tropical Medicine Center for Health SciencesFederal University of PernambucoRecife-PEBrazil

Objective: To report atypical morphological features of Trichophyton(T.) tonsurans strains associated with tinea capitis. Methods: Eighty-two T. tonsurans strains isolated in Ceará, Brazil, were analyzed regarding macro and micromorphological features and nutritional patterns. Results: fifty-two samples presented abundant chlamydoconidia, which were produced in chains. Macroscopically, these strains developed small glabrous colonies that were firmly attached to the surface of the culture medium, with few or no aerial mycelia and intense rusty yellow pigmentation. Seven strains did not grow with stimulus from thiamine. Samples were heterogeneous regarding urease production and none presented in vitro hair perforation. Conclusions: The observation of T. tonsurans strains with distinct phenotypic features indicates the need to revise the taxonomic criteria for routine identification of this dermatophyte.

关键词： Trichophyton tonsurans Chlamydoconidia Dermatophyte Phenotyping

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圣保罗的美妙假期

现代装饰（家居） 2013年第5期 42-48页

作者： f.bio Galeazzo Maíra Acayaba 肖俊巴西Galeazzo Design

本案是一套位于圣保罗郊外,面积达1900平米的乡间别墅。别墅的设计承载着一个包含几代人的大家庭休闲与聚会的功能。在选材方面,设计师尽量选用天然环保的材料对建筑进行装饰和覆盖,如:用拆迁后的废木覆盖地板和橱柜;用竹材覆盖天花和凉... 详细信息

本案是一套位于圣保罗郊外,面积达1900平米的乡间别墅。别墅的设计承载着一个包含几代人的大家庭休闲与聚会的功能。在选材方面,设计师尽量选用天然环保的材料对建筑进行装饰和覆盖,如:用拆迁后的废木覆盖地板和橱柜;用竹材覆盖天花和凉亭;而壁炉和室内的某些墙面则用糙石铺设。主厅设置的一根老旧的大型圆木被打磨光滑,一道用考顿钢打造的螺旋式楼梯围绕其盘旋而上。

关键词：天然环保圆木土耳其浴室室内设计蒸气室干蒸 restaurant 按摩室 heated bamboo