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In situ electron holography of.the dynamic magnetic f.eld emanating f.om a hard-disk drive writer

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Nano Research 2015年第4期8卷 1241-1249页

作者： Joshua f. Einsle Christophe Gatel Aurelien Masseboeuf.Robin Cours Muhammad A. Bashir Mark Gubbins Robert M. Bowman Etienne Snoeck Centre f.r Nanostructured Media School of Mathematics and Physics Queen's University Belfast Belfast BT7 1NN UK CEMES-CNRS 29 rueJ. Marvig 31055 Toulouse France Universite de Toulouse Paul Sabatier 118 route de Narbonne 31062 Toulouse Cedex 9 France Seagate Technology I Disc Drive Springtown Industrial Estate Derry City Co. BT48 OBF Londonderry UK

The prolif.ration of.mobile devices in society accessing data via the ＂cloud＂ is imposing a dramatic increase in the amount of.inf.rmation to be stored on hard disk drives （HDD） used in servers. f.recasts are that areal densities will need to increase by as much as 35% compound per annum and by 2,020 cloud storage capacity will be around 7 zettabytes corresponding to areal densities of.2 Tb/in^2. This requires increased perf.rmance f.om the magnetic pole of.the electromag- netic writer in the read/write head in the HDD. Current state-of.art writing is undertaken by morphologically complex magnetic pole of.sub 100 nm dimensions, in an environment of.engineered magnetic shields and it needs to deliver strong directional magnetic f.eld to areas on the recording media around 50 nm × 13 nm. This points to the need f.r a method to perf.rm direct quantitative measurements of.the magnetic f.eld generated by the write pole at the nanometer scale. Here we report on the complete in situ quantitative mapping of.the magnetic f.eld generated by a f.nctioning write pole in operation using electron holography. The results point the way towards a new nanoscale magnetic f.eld source to f.rther develop in situ transmission electron microscopy.

关键词： electron transmissionmicroscopy of.-axis electronholography in situ magnetic f.eld magnetic recording hard disk write pole

The integrated use of.chemical insecticides and the entomopathogenic nematode, Steinemema carpocapsae （Nematoda： Steinernematidae）, f.r the control of.sweetpotato whitef.y, Bemisia tabaci （Hemiptera： Aleyrodidae）

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Insect Science 2008年第5期15卷 447-453页

作者： Andrew G. S. Cuthbertson James J. Mathers Phil Northing Anthony J. Prickett Keith f. A. Waiters Central Science Laboratory Sand Hutton York YO41 1LZ UK

The integration of.chemical insecticides and inf.ctive juveniles of.the entomopathogenic nematode Steinernema carpocapsae （Wesier）（Nematoda： Steinernematidae）, to control second instars of.the sweetpotato whitef.y, Bemisia tabaci Gennadius （Hemiptera： Aleyrodidae） was investigated. Using a sand bioassay, the ef.ects of.direct exposure of.S. carpocapsae f.r 24 h to f.eld rate dilutions of.f.ur insecticides （spiromesif.n, thiacloprid, imidacloprid and pymetrozine） on inf.ctivity to Galleria mellonella larvae were tested. Although all chemicals tested, except spiromesif.n, produced acceptable nematode inf.ctivity rates, they were all signif.cantly less than the water control. The ef.ect of.insecticide treatment （dry residues of.spiromesif.n, thiacloprid and pymetrozine and soil drench of.imidacloprid） on the ef.icacy of.the nematode against B. tabaci was also investigated. Nematodes in combination with thiacloprid and spiromesif.n gave higher B. tabaci mortality （86.5% and 94.3% respectively） compared to using nematodes alone （75.2%） on tomato plants. There was no signif.cant dif.erence in B. tabaci mortality when using the chemicals imidacloprid, pymetrozine and spiromesif.n in conjunction with nematodes compared to using the chemicals alone. However, using thiacloprid in combination with the nematodes produced signif.cantly higher B. tabaci mortality than using the chemical alone. The integration of.S. carpocapsae and these chemical agents into current integrated pest management programmes f.r the control of.B. tabaci is discussed.

关键词： entomopathogenic nematode inf.ctivity insecticide residue pest management

11种常见支原体环介导等温扩增检测方法的建立及验证

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中国生物制品学杂志 2023年第8期36卷 980-986页

作者：柯晓梅吴宇曼孔伟圣陈智妍刘帅吴华佐朱霞燕张健遵义医科大学珠海校区生物工程系广东珠海519041 珠海贝索细胞科学技术有限公司广东珠海519000

目的建立一种用于同时检测多种支原体污染的环介导等温扩增(loop-mediated isothermal amplif.cation,LAMP)法,并进行验证。方法通过SnapGene软件的多序列比对工具比对13种支原体16S rRNA基因的保守序列,根据保守序列设计2条内引物f.P... 详细信息

目的建立一种用于同时检测多种支原体污染的环介导等温扩增(loop-mediated isothermal amplif.cation,LAMP)法,并进行验证。方法通过SnapGene软件的多序列比对工具比对13种支原体16S rRNA基因的保守序列,根据保守序列设计2条内引物f.P和BIP、2条外引物f.和B3、环引物LOOP(Lf.LB);建立LAMP体系:Bst DNA聚合酶大片段(8 U/μL)、10×ThermoPoly反应缓冲液[20 mmol/L Tris-HCl,10 mmol/L KCl,10 mmol/L(H4)2SO4,2 mmol/L MgSO4,0.1%Triton X-100,pH 8.8]、MgSO4(100 mmol/L)、dNTP Mix、f.P/BIP Primers、f./B3 Primers、LOOP Primers、无核酸酶水、模板DNA(13种搭载支原体16S rRNA的质粒)。产物进行1%琼脂糖凝胶电泳鉴定。验证方法可行性、特异性、可重复性,确定方法检测限。结果筛选的2组LAMP引物,组合使用可同时检测出11种支原体序列;在其他非特异模板检测中未出现非特异扩增,引物组特异性良好;建立的方法灵敏度好,最低检测限为30.1 copies/μL,且重复性好,未见假阳性和假阴性扩增。结论 LAMP技术可用于检测细胞培养中多种支原体污染的情况,特异性好、耗时短,可作为细胞培养中快速检测支原体污染的技术平台之一。

关键词：环介导等温扩增支原体 16S rRNA

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系统性红斑狼疮患者的痫性发作

世界核心医学期刊文摘（神经病学分册） 2005年第4期1卷 38-39页

作者： Appenzeller S. Cendes f. Costallat L.T.L. 袁海峰 Department of.Neurology State Univ. of Campinas (UNI- CAMP) Cd. Universitaria Zeferino Vaz CEP 13083970 Campinas- SP Brazil Dr

Objective: To evaluate the f.equency and risk f.ctors of.epileptic seizures in a large cohort of.patients with systemic lupus erythematosus (SLE). Methods: f.ve hundred nineteen consecutive patients with SLE were studied, with f.llow- up ranging f.om 4 to 7.8 years. The type and f.equency of.risk f.ctors associated with acute and recurrent epileptic seizures in SLE were determined. Results: Sixty (11.6% ) patients with epileptic seizures were identif.ed. Epileptic seizures occurred at the onset of.SLE symptoms in 19 (31.6% ) and af.er the onset of.SLE in 41 of.60 (68.3% ) patients. f.f.y- three of.60 (88.3% )- patients had acute symptomatic epileptic seizures, and 7 of.60 (11.7% ) had recurrent epileptic seizures. Variables associated with acute epileptic seizures at SLE onset were stroke (p = 0.0004) and antiphospholipid antibodies (p = 0.0013). Epileptic seizures during f.llow- up were related to nephritis (p = 0.001), antiphospholipid antibodies (p = 0.005), and epileptic seizures at disease onset (p = 0.00001). All seven patients who presented recurrent epileptic seizures had antiphospholipid syndrome and interictal epileptic abnormalities on EEG. Conclusions: Epileptic seizures were observed in 11.2% of.systemic lupus erythematosus (SLE) patients. Antiphospholipid antibodies and stroke were related to epileptic seizures at SLE disease onset. Patients with renal f.ares, epileptic seizures at SLE disease onset, and antiphospholipid antibodies were at greater risk f.r acute symptomatic seizures during f.llow- up. Recurrence of.epileptic seizures occurred in 1.3% of.patients and was associated with antiphospholipid syndrome.

关键词：系统性红斑狼疮痫性发作抗磷脂抗体发作间期性异常

多电荷离子双电子复合中的宇称不守恒效应(英文)

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复旦学报（自然科学版） 2007年第1期46卷 50-53页

作者： M G Kozlov G f.Gribakin f.J Currell Petersburg Nuclear Physics Institute Gatchina 188300 Russia School of.Mathematics and Physics Queen's University Belfast BT7 1NNNorthern Ireland United Kingdom

A parity nonconserving (PNC) asymmetry in the cross section f.r dielectronic recombination of.polarized electrons on multiply charged ions with Z≥40 is discussed. This ef.ect is strongly enhanced f.r close doubly-excited states of.opposite parity in the intermediate compound ion. Such states are known f.r He-like ***, these levels have large energy and large radiative widths which hampers observation of.the PNC asymmetry. We argue that accidentally degenerate states of.more complex ions may be more suitable f.r the corresponding experiment.

关键词：奇偶不守恒不对称二维电子重组带电离子激发状态

先天性巨结肠的诊断:关于常用检测方法准确性对比的前瞻性研究

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世界核心医学期刊文摘（儿科学分册） 2006年第2期2卷 23-24页

作者： De Lorijn f. Reitsma J.B. Voskuijl W.P. 郭战宏 Department of.Pediatric Gastroenterology and Nutrition Emma Children' s Hospital AMC/Academic Medical CenterMeibergdreef 9 1105AZ Amsterdam Netherlands Dr.

Objective: To compare the diagnostic accuracy of.contrast enema (CE), anorectal manometry (ARM), and rectal suction biopsy (RSB) f.r the detection of.Hirschsprung’ s disease (HD). Study design: f.llowing a prospective pro-tocol, inf.nts suspected of.HD underwent all 3 index tests. Children with positive results on 2 or more index tests or who continued to have severe bowel problems underwent a f.ll thickness biopsy as ref.rence standard. Clinical f.llow-up was the ref.rence standard in all other children. Results: Between 2000 and 2003, 111 consecutive patients (67 boys; median age, 5.3 months) in whom HD was suspected were enrolled. HD was f.und in 28 patients. RSB had the highest sensitivity (93% ) and specif.city (100% ) rates, but values were not signif.cantly dif.erent f.om CE (sensitivity, 76% ; specif.city, 97% ) or f.om ARM (sensitivity, 83% ; specif.city, 93% ). Inconclusive test results occurred in 8 inf.nts with CE, in 15 inf.nts with ARM because of.agitation, and in 2 inf.nts with RSB. Conclusion: RSB is the most accurate test f.r diagnosing HD, and it has the lowest rate of.inconclusive test results.

关键词：诊断准确性先天性巨结肠检测结果前瞻性研究肛门直肠测压肠道功能障碍显著性差异敏感性灌肠造影阳性结果

环境条件调控植物挥发性有机物的排放与其对成云的影响

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环境条件调控植物挥发性有机物的排放与其对成云的影响

第35届中国气象学会年会

作者：赵德峰 Buchholz, A. Tillmann, R. Kleist, E. Wu, C. Rubach, f. Kiendler-Scharr, A. Rudich, Y. Wildt, J. Mentel, T. f. 复旦大学大气与海洋科学系/大气科学研究院

陆生植被排放大量的挥发性有机物（VOC）进入大气。这些挥发性有机物在大气中氧化生成二次有机气溶胶。通过作为云凝结核,二次有机气溶胶可参与成云,影响气候。随着全球变暖,各种环境因素可以对植物造成胁迫,从而诱导植物VOC排放的变化... 详细信息

陆生植被排放大量的挥发性有机物（VOC）进入大气。这些挥发性有机物在大气中氧化生成二次有机气溶胶。通过作为云凝结核,二次有机气溶胶可参与成云,影响气候。随着全球变暖,各种环境因素可以对植物造成胁迫,从而诱导植物VOC排放的变化,进而可能改变气溶胶的粒径与化学组成。本工作针对寒带森林,选取昆虫侵扰和高温作为生物和非生物胁迫的代表,研究了环境因素如何影响植物诱导性VOC排放进而影响二次有机气溶胶的成云活性。研究发现昆虫侵扰可造成新型的VOC排放,进而改变二次有机气溶胶的组成和吸湿性因子。中度的高温可以提高VOC的排放总量,对气溶胶的吸湿性因子影响很小,但通过改变气溶胶的粒径而改变成云活性。植物胁迫、VOC化学组成和二次有机气溶胶成云活性之间的耦合作用表明诱导性VOC排放可对成云和气候产生重要影响。

关键词：挥发性有机物二次有机气溶胶成云活性吸湿性因子陆生植物实验室模拟

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战神归来

丝氨酸蛋白酶活性和残留LEKTI的表达决定Netheron综合征的表型

军事世界画刊 2011年第7期 58-59页

作者：克里斯托弗·f.福斯魏兆岗

最近十多年来,机动性能出色、更具作战弹性的自行火炮备受欧洲国家的青睐,出现了一股大量采购自行火炮的热潮.但很多亚洲国家因为地形条件和财力的限制,通常在少量装备自行火炮系统的同时,仍大量列装环境适应能力更强、价格更低廉的牵...

最近十多年来,机动性能出色、更具作战弹性的自行火炮备受欧洲国家的青睐,出现了一股大量采购自行火炮的热潮.但很多亚洲国家因为地形条件和财力的限制,通常在少量装备自行火炮系统的同时,仍大量列装环境适应能力更强、价格更低廉的牵引式火炮.西方军火商历来重视亚洲市场,牵引式火炮自然也是他们的重点市场之一,法国Nexter系统公司的LGI型轻型火炮和英国BAE系统公司的105毫米火炮非常适合亚洲国家使用,在亚洲火炮市场上占有很大的市场份额.此外,美国和俄罗斯等国也对亚洲牵引火炮市场虎视眈眈,希望能在这块大蛋糕上狠狠的切下属于自己的一块.

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世界核心医学期刊文摘（皮肤病学分册） 2006年第10期2卷 18-19页

作者： Hachem J.-P. Wagberg f. Schmuth M. 党倩丽 Dienst Dermatologie and Toxicologie Academisch Ziekenhuis-Vrije Universiteit BrusselLaarbeeklaan 101Brussel 1090Belgium Dr.

Mutations in the SPINK5 gene encoding the serine protease(SP)inhibitor,lymphoepithelial-Kazal-type 5 inhibitor(LEKTI),cause Netherton syndrome(NS),a lif.-threat-ening disease,owing to proteolysis of.the stratum corneum(SC).We assessed here the basis f.r phenotypic variations in nine patients with “mild”,“moderate”,and “severe”*** magnitude of.SP activation correlated with both the barrier def.ct and clinical severity,and inversely with residual LEKTI *** co-localizes within the SC with kallikreins 5 and 7 and inhibits both *** permeability barrier abnormality in NS was f.rther linked to SC thinning and proteolysis of.two lipid hydrolases(β-glucocerebrosidase and acidic sphingomyelinase),with resultant disorganization of.extracellular lamellar *** attenuation correlated with phenotype-dependent,SP activation,and loss of.corneodesmosomes,owing to desmoglein(DSG)1 and desmocollin(DSC)1 *** excess SP activity extended into the nucleated layers in NS,degrading desmosomal mid-line structures with loss of.DSG1/DSC1,the integrity of.the nucleated epidermis appears to be maintained by compensatory upregulation of.DSG3/*** of.suf.icient permeability barrier f.nction f.r survival correlated with a compensatory acceleration of.lamellar body secretion,providing a partial permeability barrier in *** studies provide a mechanistic basis f.r phenotypic variations in NS,and describe compensatory mechanisms that permit survival of.NS patients in the f.ce of.unrelenting SP attack.

关键词： LEKTI Netheron 蛋白酶活性胞外膜激肽释放酶原板层小体渗透屏障防御屏障桥粒