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Neurolin expression in the optic nerve and immunoreactivity of Pax6-positive niches in the brain of rainbow trout(Oncorhynchus mykiss) after unilateral eye injury

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Neural Regeneration Research 2019年第1期14卷 156-171页

作者： evgeniya v.pushchina Anatoly A.varaksin National Scientific Center of Marine Biology Far Eastern Branch Russian Academy of Sciences A.A.Bogomoletz Institute of Physiology National Academy of Sciences of Ukraine

In contrast to astrocytes in mammals, fish astrocytes promote axon regeneration after brain injury and actively participate in the regeneration process. Neurolin, a regeneration-associated, Zn8-1abeled protein, is involved in the repair of damaged optic nerve in goldfish. At 1 week after unilateral eye injury, the ex- pression of neurolin in the optic nerve and chiasm, and the expression of Pax6 that influences nervous system development in various brain regions in the rainbow trout （Oncorhynchus mykiss） were detected. Immunohistochemical staining revealed that the number of Zn8~ cells in the optic nerve head and intraor- bital segment was obviously increased, and the increase in Zn8~ cells was also observed in the proximal and distal parts of injured optic nerve. This suggests that Zn8＊ astrocytes participate in optic nerve regeneration. ELISA results revealed that Pax6 protein increased obviously at 1 week post-injury. Immunohistochemi- cal staining revealed the appearance of Pax6＋ neurogenic niches and a larger number of neural precursor cells, which are mainly from Pax6＋ radial glia cells, in the nuclei of the diencephalon and optic tectum of rainbow trout （Oncorhynchus mykiss）. Taken together, unilateral eye injury can cause optic nerve reaction, and the formation of neurogenic niches is likely a compensation phenomenon during the repair process of optic nerve injury in rainbow trout （Oncorhynchus mykiss）.

关键词： optic nerve injury neurolin Zn8 Pax6 rainbow trout (Oncorhynchus mykiss) radial ilia cells microenvironment neural regeneration

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Cell proliferation and apoptosis in optic nerve and brain integration centers of adult trout Oncorhynchus mykiss after optic nerve injury

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Neural Regeneration Research 2016年第4期11卷 578-590页

作者： evgeniya v.pushchina Sachin Shukla Anatoly A.varaksin Dmitry K.Obukhov Laboratory of Cytophysiology A.V.Zhirmunsky Institute of Marine Biology Far Eastern Branch of Russian Academy of Sciences Prof.Brien Holden Eye Research Centre L.V.Prasad Eye Institute St.Petersburg State University Universitetskaya

Fishes have remarkable ability to effectively rebuild the structure of nerve cells and nerve fibers after central nervous system ***,the underlying mechanism is poorly *** order to address this issue,we investigated the proliferation and apoptosis of cells in contralateral and ipsilateral optic nerves,after stab wound injury to the eye of an adult trout Oncorhynchus *** population of proliferating cells was investigated at 1 week after *** labeling gave a qualitative and quantitative assessment of apoptosis in the cells of optic nerve of trout 2 days after *** optic nerve injury,apoptotic response was investigated,and mass patterns of cell migration were *** maximal concentration of apoptotic bodies was detected in the areas of mass clumps of *** is probably indicative of massive cell death in the area of high phagocytic activity of macrophages/*** 1 week after optic nerve injury,we observed nerve cell proliferation in the trout brain integration centers：the cerebellum and the optic *** the optic tectum,proliferating cell nuclear antigen（PCNA）-immunopositive radial glia-like cells were *** activity of nerve cells was detected in the dorsal proliferative（matrix） area of the cerebellum and in parenchymal cells of the molecular and granular layers whereas local clusters of undifferentiated cells which formed neurogenic niches were observed in both the optic tectum and cerebellum after optic nerve *** vitro analysis of brain cells of trout showed that suspension cells compared with monolayer cells retain higher proliferative activity,as evidenced by PCNA *** contrast observation showed mitosis in individual cells and the formation of neurospheres which gradually increased during 1–4 days of *** present findings suggest that trout can be used as a novel model for studying neuronal regeneration.

关键词： nerve regeneration proliferation apoptosis optic nerve brain radial glia cells neurogenic niches neurospheres neural regeneration

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GFAP expression in the optic nerve and increased Н2S generation in the integration centers of the rainbow trout（Oncorhynchus mykiss） brain after unilateral eye injury

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Neural Regeneration Research 2020年第10期15卷 1867-1886页

作者： evgeniya v.pushchina Anatoly A.varaksin Dmitry K.Obukhov Igor M.Prudnikov A.v.Zhirmunsky National Scientific Center of Marine Biology Far Eastern BranchRussian Academy of SciencesVladivostokRussia A.A.Bogomoletz Institute of Physiology National Academy of Sciences of UkraineKievUkraine St.Petersburg State University St.PetersburgRussia

Hydrogen sulfide(H2S)is considered as a protective factor against cardiovascular ***,there are few reports on the effects of H2S in the central nervous system during stress or *** studies on goldfish have shown that astrocytic response occurs in the damaged and contralateral optic *** fibrillary acidic protein(GFAP)concentration in the optic nerves of rainbow trout has not been measured *** study further characterized the astrocytic response in the optic nerve and the brain of a rainbow trout(Oncorhynchus mykiss)after unilateral eye injury and estimated the amount of H2S-producing enzyme cystathionineβ-synthase(CBS)in the brain of the rainbow *** 1 week after unilateral eye injury,a protein band corresponding to a molecular weight of 50 kDa was identified in the ipsi-and contralateral optic nerves of the rainbow *** concentration of GFAP in the injured optic nerve increased compared to the protein concentration on the contralateral *** results of a quantitative analysis of GFAP+cell distribution in the contralateral optic nerve showed the largest number of GFAP+cells and fibers in the optic nerve *** the damaged optic nerve,patterns of GFAP+cell migration and large GFAP+bipolar activated astrocytes were detected at 1 week after unilateral eye *** study of H2S-producing system after unilateral eye injury in the rainbow trout was conducted using enzyme-linked immunosorbent assay,western blot analysis,and immunohistochemistry of polyclonal antibodies again st CBS in the integrative centers of the brain:telencephalon,optic tectum,and ***-linked immunosorbent assay results showed a 1.7-fold increase in CBS expression in the rainbow trout brain at 1 week after unilateral eye injury compared with that in intact *** the ventricular and subventricular regions of the rainbow trout telencephalon,CBS+radial glia and neuroepithelial cells were *** unilateral eye injury

关键词： astrocyte-like cells glial fibrillary acidic protein hydrogen sulfide neuroepithelial cells neuroprotection radial glial cells rainbow trout(Oncorhynchus mykiss) reactive oxygen species unilateral eye injury

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A system-integrated approach for the design of tourist areas at the local level under changing conditions:A case study in the Altay Mountains

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Regional Sustainability 2024年第2期5卷 89-102页

作者： Alexandr Nikolaevich DUNETS evgeniya Mikhailovna TABAKAEvA HAN Fang Department of Scientific and Innovative Development Altai State UniversityBarnaul656049Russia Department of Recreational Geography ServiceTourism and HospitalityInstitute of GeographyAltai State UniversityBarnaul656049Russia State Key Laboratory of Desert and Oasis Ecology Key Laboratory of Ecological Safety and Sustainable Development in Arid LandsXinjiang Institute of Ecology and GeographyChinese Academy of SciencesUrumqi830011China

The existing approaches for the design of tourist areas often lead to limited flexibility in project *** realize a more flexible approach,in this study,we formulated a model for planning and designing tourist areas at the local ***,specific tools for analyzing tourist areas and ensuring sustainable development under changing conditions were *** study was conducted in two tourist regions,Biryuzovaya Katun tourist complex and Belokurikha destination(including Belokurikha City with Belokurikha 2 Gornaya tourist complex and surrounding areas),in the Altay *** employed the recreation opportunity spectrum and proposed a system-integrated programming approach for the design of tourist areas at the destination and site *** key of this approach was the collection and analysis of current spatial data,including the spatial distribution of attractions and visitor *** constructed heat maps using video recording and unmanned aerial vehicle(UAv)observation ***,we analyzed the video stream using an image-analyzing framework You Only Look Once(YOLO)v5 *** heat map of visitor flows based on video recording data in the Andreevskaya Sloboda museum of Belokurikha 2 Gornaya tourist complex allowed us to highlight the most attractive sites in this area and classify them into one of three types:points of functional concentration,points of transitional concentration,and points of *** heat maps in Biryuzovaya Katun tourist complex,created using UAv observation data,allowed us to determine the spatiotemporal patterns of visitor flows and tourists’preferences throughout the day within four time intervals:09:00-12:00,13:00-14:00,14:00-15:00,and 16:00-18:00(LST).The maximum visitor flow density occurred from 16:00 to 18:00 in the beach area of the artificial lake.A comparison between the visitor-concentrated sites and the current facilities provided insights into the demand for attractions and facilities and

关键词： Tourism planning Tourist areas Unmanned aerial vehicle(UAv) visitor flows Heat map Altay Mountains

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Features of adult neurogenesis and neurochemical signaling in the Cherry salmon Oncorhynchus masou brain

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Neural Regeneration Research 2013年第1期8卷 13-23页

作者： evgeniya v. pushchina Dmitry K. Obukhov Anatoly A. varaksin Laboratory of Cytophysiology Institute of Marine Biology Far East Branch Russian Academy of Sciences St. Petersburg State University

We investigated the distribution of gamma aminobutyric acid, tyrosine hydroxylase and nitric oxide-producing elements in a cherry salmon Oncorhynchus masou brain at various stages of postnatal ontogenesis by immunohistochemical staining and histochemical staining. The periventricular region cells exhibited the morphology of neurons and glia including radial glia-like cells and contained several neurochemical substances. Heterogeneous populations of tyrosine hydroxylase-, gamma aminobutyric acid-immunoreactive, as well as nicotinamide adenine dinucleotide phosphate diaphorase-positive cells were observed in proliferating cell nuclear antigen-immunoreactive proliferative zones in periventricular area of diencephalon, central grey layer of dorsomedial tegmentum, medulla and spinal cord. Immunolocalization of Pax6 in the cherry salmon brain revealed a neuromeric construction of the brain at various stages of postnatal ontogenesis, and this was confirmed by tyrosine hydroxylase and gamma aminobutyric acid labeling.

关键词： neural regeneration neurogenesis teleostei adult neurogenesis neurotransmitter signaling migration tyrosine hydroxylase gamma aminobutyric acid development Pax6 NADPH-diaphorase proliferation grant-supported paper neuroregeneration

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Hydrogen Sulfide in Proliferating and Differentiated Cells in Primary Cultures of Juvenile Brain of Masu Salmon <i>Oncorhynchus masou</i>

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Advances in Bioscience and Biotechnology 2015年第8期6卷 539-545页

作者： evgeniya v. pushchina Sachin Shukla Anatoly A. varaksin Zhirmunsky Institute of Marine Biology Far East Branch Russian Academy of Sciences Vladivostok Russia Prof. Brien Holden Eye Research Centre L.V. Prasad Eye Institute Hyderabad India

Analysis of proliferative activity and the ability to neuron differentiation was performed in cultured cells of the brain and spinal cord of juvenile masu salmon Oncorhynchus masou. Proliferating cell nuclear antigen (PCNA) was used as a proliferative marker, while the markers of neuronal differentiation—a neuron protein HuCD, and a neuron-specific transcriptional factor with two DNA- binding sites Pax6—detected neurons. The results showed that cell proliferation occurred mainly in the suspension cell fraction. In monolayer, a few cells were only found to express PCNA. The results of morphological and immunohistochemical analysis allow us to conclude that proliferative activity in primary cultures from the O. masou brain is mainly connected with the suspension fraction of small cells. In contrast, a positive correlation between the cells expressing cystathionine β-synthase (CBS), a marker of H2S synthesis, and the cells expressing PCNA in the monolayer, indicates the participation of H2S in proliferative activity of neurons in primary cultures. The data obtained suggest that the hydrogen sulphide is also involved in the process of differentiation.

关键词： Cell Culture Fish PCNA HuCD Hydrogen Sulfide Pax6 Regeneration Adult Neurogenesis

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Trace amine-associated receptors at the cross-road between innate olfaction of amines, emotions, and adult neurogenesis

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Neural Regeneration Research 2022年第6期17卷 1257-1258页

作者： evgeniya v.Efimova Nataliia v.Katolikova Evgeny v.Kanov Raul R.Gainetdinov Institute of Translational Biomedicine and Saint Petersburg University Hospital Saint Petersburg State UniversitySaint PetersburgRussia

Trace amines are the class of endogenous biogenic amines that traditionally include beta-phenylethylamine,p-tyramine,tryptamine,octopamine,and o *** t race amines represent products of amino acids decarboxylation by bacterial decarboxylases during tissue putrefaction or by endogenous decarboxylases in the *** of trace amines by gut microbiota is also known(Berry et al.,2017;Gainetdinov et al.,2018).Thus,trace amines are enriched during the decomposition of proteins and concentrated in certain bodily fluids.

关键词： amines Berry concentrated

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Nitric oxide-factor, which regulates proliferation and apoptosis in the adult brain of amur sturgeon <i>Acipenser schrenckii</i>

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Advances in Bioscience and Biotechnology 2012年第6期3卷 788-804页

作者： Е. v. pushchina D. К. Obukhov A.v. Zhyrmunskii Institute of Marine Biology Far East Division Russian Academy of Sciences Vladivostok Russia St.Petersburg State University St. Petersburg Russia

The distribution of proliferative zones, NO-producing cells and apoptosis areas in the medulla oblongata, cerebellum, optic tectum, thalamus and hypothalamus of Amur sturgeon Acipenser schrenckii was investigated, using techniques of immunoperoxidase staining of proliferating cell nuclear antigen (PCNA), neuronal nitric oxide synthase and TU-NEL-labeling of fragmented DNA. It has been established, that in the sturgeon brain NO can act both as a cytotoxic proapoptogenic factor, and as a factor, which stimulates cell proliferation. The presence of NO-producing elements in somato- and viscerosensory areas of medulla oblongata, tectum, cerebellum and thalamus suppose, that in these brain areas NO constitutes apoptogenic factor, which induces the cells death in a territory of postmitotic neuroblasts, renders controlling effect on development and differentiating of chemosensory, visual, motor and hypophysotropic brain areas in postnatal ontogenesis. Maximal proliferating activity and high concentration of NO-ergic cells were revealed in external layers, adjoining to the medullar, cerebellar and tectum membranes, that allow to suppose NO participation in postnatal morphogenesis of these brain structures as a factor, which regulates cell proliferation. In sensory centers (tectum and nuclei of the v, vII, and X nerves), significantly varying ratios of intensities of proliferation and apoptosis were found;this is indicative of dissimilar rates of growth and differentiation in visual and chemosensory centers of the sturgeon brain. Presence of NO-producing elements in the PCNA- immuno-labeling and TUNEL-labeling brain areas allow to consider NO as a factor, which balances processes of proliferation and apoptosis in the sturgeon brain.

关键词： Adult Neurogenesis Nitric Oxide Apoptosis Sturgeon Neotenia Development of Sensory Systems

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Mesoscopic Models of Plants Composed of Metallic Nanowires

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Journal of Bionic Engineering 2013年第3期10卷 368-376页

作者： Galina K. Strukova Gennady v. Strukov evgeniya Yu. Postnova Alexander Yu. Rusanov Ivan S. veshchunov Institute of Solid State Physics Russian Academy of Sciences 142432 Chernogolovka Russia

various metallic structures of complex shape resembling living plant organisms （biomimetics） are produced as a result of self-assembly of nanowires growing on porous membranes in the course of pulse current electrodeposition. These structures occur if the electroplating is continued after the nanowires appear on the membrane surface. By varying the membrane ge- ometry, pulse current electroplating parameters, and alternating electrodeposition from two baths composed of a variety of electrolytes, diverse models were fabricated, including a hollow container with a wall thickness of 10 nm 20 nm. This biomimetic method suggests an analogy between the shape-forming processes of plants and their metallic models. Nanostruc- tured mesostructures of various metals （Ag, Pd, Ni）, alloys （PdNi, Pbln） and hybrid structures .（PdNi/Pb, PdNi/Pbln） were obtained. They can be of interest for fundamental research （self-assembly, morphogenesis） as well as for applications in nanotechnology （catalysis, nanoplasmonics, medicine, superhydrophobic surfaces）.

关键词： templated growth pulse electroplating self-assembly metallic mesostructures nanowires fractal branching biomimetic method

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Interaction of Atoms with Grain Surfaces in Steel: Periodic Dependence of Binding Energy on Atomic Number and Influence on Wear Resistance

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Engineering（科研） 2012年第10期4卷 76-79页

作者： E. v. pushchina D. K. Obukhov A. v. Zhirmunski Institute of Marine Biology Far East Division Russian Academy of Sciences ul. Pal'chevskii 17 Vladivostok 690059 Russia ru St. Petersburg State University Universitetskaya Naberezhnaya 7/9 St. Petersburg 199034 Russia

The data of our investigations contribute to understanding of cellular mechanisms of the teleost fishes CNS forming in postembryonic development. The revealed peculiarities of structural and neurochemical organization and description of basic histogenetic processes (proliferation, migration and neuronal cell differentiation) during the brain forming in fish, which have signs of fetal organization, widen the existing knowledge about histogenesis of these structures in postembryonic development. It seems conceivable, that during postembrional development in teleost fishes some neurotransmitters and gaseous mediators (NO and H2S) act as factors, which initiate and regulate the cellular and the tissues processes of genetic program during the brain development. Materials of this investigation define a new experimental model for studying of postembrional neurogenesis processes.

关键词： Teleostei Postnatal Neurogenesis Proliferative Cell Nuclear Antigen (PCNA) Neurotransmitter Signaling Migration Tyrosine Hydroxylase GABA Development Pax6 NADPH-diaphorase Nitric Oxide Hydrogen Sulfide Proliferation.

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