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Variation in SARS-CoV-2 seroprevalence in children in the region of Asturias,Northern Spain

World Journal of Pediatrics 2022年第12期18卷 835-844页

作者： Elisa García-García mercedes Rodríguez-Pérez David Pérez-Solís Carlos Pérez-méndez Cristina molinos-Norniella Ángeles Cobo-Ruisánchez eva maría Fernández Fernández Noelia García González Laura Calle-miguel Pediatrics Department Centro de Salud LaviadaCalle Juanín de Mieres833207GijónSpain microbiology Department Hospital Universitario Central de AsturiasOviedoSpain Pediatrics Department Hospital Universitario San AgustínAvilésSpain Pediatrics Department Hospital Universitario de CabueñesGijónSpain Pediatrics Department Hospital Carmen y Severo Ochoa.Cangas del NarceaSpain Pediatrics Department Centro de Salud TeatinosOviedoSpain Pediatrics Department Hospital Universitario Central de AsturiasOviedoSpain

Background Updated seroprevalence estimates are important to describe the severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)landscape and to guide public health *** aims are to describe longitudinal changes in seroprevalence in children in a region in Northern Spain and to analyze factors associated with SARS-CoV-2 *** Prospective multicenter longitudinal study with subjects recruited from July to September ***(up to 14 years old)were included and followed up until September *** blood samples were collected every six months during three testing rounds and were analyzed for SARS-CoV-2 *** data regarding epidemiological features,contact tracing,symptoms,and virological tests were *** evolution of SARS-CoV-2 seroprevalence during the study and the differences between children with positive and negative SARS-CoV-2 antibody tests were *** Two hundred children were recruited(50.5%girls,median age 9.7 years).The overall seroprevalence increased from round 1[1.5%,95%confidence interval(CI)0.3%–4.3%]to round 2(9.1%,95%CI 4.6%–12.7%)and round 3(16.6%,95%CI 9.5%–19.6%)(Pmain changes occurred in children aged zero to four years(P=0.001)who lived in urban areas(Pme *** multivariable analysis,three variables independently associated with SARS-CoV-2 seropositivity were identified:close contact with coronavirus disease 2019(COVID-19)confirmed or suspected cases[odds ratio(OR)=3.9,95%CI 1.2–12.5],previous positive virological test(OR=17.1,95%CI 3.7–78.3)and fatigue(OR=18.1,95%CI 1.7–193.4).Conclusions SARS-CoV-2 seroprevalence in children has remarkably increased during the time of our *** was the only COVID-19-compatible symptom that was more frequent in seropositive than in seronegative children.

关键词： Antibodies Child COVID-19 Seroepidemiologic studies

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maize seed coatings and seedling sprayings with chitosan and hydrogen peroxide:their influence on some phenological and biochemical behaviors

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2013年第2期14卷 87-96页

作者： eva-Guadalupe LIZRRAGA-PAULíN Susana-Patricia mIRANDA-CASTRO Ernesto mORENO-mARTíNEZ Alma-Virginia LARA-SAGAHóN Irineo TORRES-PACHECO Laboratorio de Biotecnología Facultad de Estudios Superiores CuautitlánCampo 1Universidad Nacional Autónoma de MéxicoEstado de México 54740México Unidad de Investigación en Granos y Semillas Facultad de Estudios Superiores CuautitlánCentro de Asimilación TecnológicaUniversidad Nacional Autónoma de MéxicoEstado de México 54740México CA Ingeniería de Biosistemas División de Investigación y PosgradoFacultad de IngenieríaUniversidad Autónoma de QuerétaroQuerétaro 76010México

Objective： To evaluate the effect of chitosan （CH） and hydrogen peroxide （H2O） seed coatings and seedling sprinklings on two different maize varieties by measuring their phenology, the H202 presence, the catalase （CAT） activity, and the protein quantity. methods： Seven groups of ten seeds for each maize variety were treated with CH （2% （20 g/L） and 0.2% （2 g/L）） or H2O2 （8 mmol/L） by coating, sprinkling, or both. Germination and seedling growth were measured. One month after germination, the presence of H202 in seedlings in the coated seed treatments was evaluated. Protein content and CAT activity were determined under all treatments. Results： H2O2 seed coating en- hanced the germination rate and increased seedling and stem length in the quality protein maize （QPm） variety. Seedlings had a higher emergence velocity under this treatment in both varieties. CH and H2O2 sprinklings did not have an effect on seedling phenology. Exogenous application of H2O2 promoted an increase of endogenousH2O2. OH and H2O2seedling sprinkling increased the protein content in both maize varieties, while there was no significant effect on the CAT activity of treated seeds and seedlings. Conclusions： CH and H2O2 enhance some phenological and biochemical features of maize depending on their method of application.

关键词： Zea mays Chitosan Hydrogen peroxide Phenology Catalase Protein

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How should liver hypertrophy be stimulated?A comparison of upfront associating liver partition and portal vein ligation for staged hepatectomy(ALPPS)and portal vein embolization(PVE)with rescue possibility

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Hepatobiliary Surgery and Nutrition 2021年第1期10卷 1-8页

作者： Ernesto Sparrelid Kristina Hasselgren Bård Ingvald Røsok Peter Nørgaard Larsen Nicolai Aagaard Schultz Ulrik Carling eva Fallentin Stefan Gilg Per Sandström Gert Lindell Bergthor Björnsson Department of Clinical Science Intervention and TechnologyDivision of SurgeryKarolinska InstituteKarolinska University HospitalStockholmSweden Department of Surgery and Clinical and Experimental medicine Linköping UniversityLinköpingSweden Department of Hepato-Pancreato-Biliary Surgery Oslo University HospitalOsloNorway Department of Surgical Gastroenterology and Transplantation RigshospitaletUniversity of CopenhagenDenmark Department of Radiology and Nuclear medicin RikshospitaletOslo University HospitalOsloNorway Department of Radiology RigshospitaletUniversity of CopenhagenCopenhagenDenmark Department of Surgery Skane University HospitalLundSweden

Background:The role of associating liver partition and portal vein ligation for staged hepatectomy(ALPPS)in comparison to portal vein embolization(PVE)is *** aim of this study was to compare successful resection rates(RR)with upfront ALPPS *** with rescue ALPPS on demand and to compare the hypertrophy of the liver between ALPPS and PVE plus subsequent rescue ***:A retrospective analysis of all patients treated with PVE for colorectal liver metastasis(CRLm)or ALPPS(any diagnosis,rescue ALPPS included)at five Scandinavian university hospitals during the years 2013-2016 was conducted.A Chi-square test and a mann-Whitney U test were used to assess the difference between the groups.A successful RR was defined as liver resection without a 90-day ***:A total of 189 patients were *** RR was in 84.5%of the patients with ALPPS upfront and in 73.3%of the patients with PVE and rescue ALPPS on demand(P=0.080).The hypertrophy of the future liver remnants(FLRs)with ALPPS upfront was 71%(48-97%)compared to 96%(82-113%)after PVE and rescue ALPPS(P=0.010).Conclusions:Upfront ALPPS offers a somewhat higher successful RR than PVE with rescue ALPPS on *** sequential combination of PVE and ALPPS leads to a higher overall degree of hypertrophy than upfront ALPPS.

关键词： Associating liver partition and portal vein ligation for staged hepatectomy(ALPPS) liver surgery colorectal liver metastases(CRLm) portal vein embolization(PVE)

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The membrane-Associated Sec1/munc18 KEULE is Required for Phragmoplast microtubule Reorganization During Cytokinesis in Arabidopsis

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molecular Plant 2016年第4期9卷 528-540页

作者： Alexander Steiner Lin muller Katarzyna Rybak Vera Vodermaier eva Facher martha Thellmann Raksha Ravikumar Gerhard Wanner marie-Theres Hauser Farhah F. Assaad Botany Department School of Life Sciences Technische Universitat Munchen Emil-Ramann-Street 4 85354 Freising Germany Department Biologie I Ludwig-Maximillians Universitat 82152 Planegg-Martinsried Germany Department of Applied Genetics and Cell Biology University of Natural Resources and Life Sciences 1190 Vienna Austria

Cytokinesis, the partitioning of the cytoplasm following nuclear division, requires extensive coordination between membrane trafficking and cytoskeletal dynamics. In plants, the onset of cytokinesis is characterized by the assembly of a bipolar microtubule array, the phragmoplast, and of a transient membrane compartment, the cell plate. Little is known about the coordination between membrane deposition at the cell plate and the dynamics of phragmoplast microtubules. In this study, we monitor the localization dynamics of microtubule and membrane markers throughout cytokinesis. Our spatiotemporal resolution is consistent with the general view that microtubule dynamics drive membrane movements. Nonetheless, we provide evidence for active sorting at the cell plate and show that this is, at least in part, mediated by the TRAPPII tethering complex. We also characterize phragmoplast microtubule organization and cell plate formation in a suite of cytokinesis-defective mutants. Of four mutant lines with defects in phragmoplast microtubule organization, only morl microtubule-associated mutants exhibited aberrant cell plates. Conversely, the mutants with the strongest impairment in phragmoplast microtubule reorganization are keule alleles, which have a primary defect in membrane fusion. Our findings identify the SEC1/munc18 protein KEULE as a central regulatory node in the coordination of membrane and microtubule dynamics during plant cytokinesis.

关键词： cytokinesis cell plate phragmoplast microtubule KEULE KNOLLE PLEIADE/mAP65-3

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Immediate effects of the honda walking assist on spatiotemporal gait characteristics in individuals after stroke

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medicine in Novel Technology and Devices 2022年第4期16卷 204-211页

作者： mahyar Firouzi Emma De Keersmaecker Nina Lefeber Stijn Roggeman Erika Joos Eric Kerckhofs eva Swinnen Rehabilitation Research Group Department of PhysiotherapyHuman Physiology and AnatomyVrije Universiteit BrusselBrusselsBelgium Center for Neurosciences(C4N) Vrije Universiteit BrusselBrusselsBelgium Brussels Human Robotic Research Center(BruBotics) Vrije Universiteit BrusselBrusselsBelgium University Hospital Brussels Physical MedicineLaarbeeklaan 1011090JetteBelgium Brain Body and Cognition Research GroupFaculty of Psychology and Educational SciencesVrije Universiteit BrusselBrusselsBelgium Department of Rehabilitation Sciences Faculty of Medicine and Health SciencesGhent UniversityGhentBelgium

The use of unconstrained lower limb exoskeletons has become a promising approach to assist individuals with gait *** Honda Walking Assist(HWA)is a hip-assistive exoskeleton functioning as a gait trainer and has been shown to improve several gait related outcomes after *** investigating its immediate effects on spatiotemporal gait parameters other than walking speed in stroke survivors are *** aim of this study was to investigate immediate differences in spatiotemporal gait parameters of stroke survivors between normal overground walking,walking with an unpowered,non-assisting HWA and walking with an optimally assisting *** ischemic stroke survivors(mean time since stroke 115±213.6 days)walked 3 times 5 m in each *** in 14 spatiotemporal gait parameters between all 3 conditions were registered and reported in a descriptive *** optimal assistance,4 patients walked faster(0.057–0.095 m/s)with longer strides of the paretic(0.055–0.069 m)and non-paretic(0.053–0.077 m)leg compared to normal *** to unpowered walking,all patients walked faster(0.020–0.063 m/s)in the optimal assist condition,with longer strides of the paretic(0.036–0.072 m)and non-paretic leg(0.045–0.082 m).During unpowered walking,gait velocity remained unchanged in 2 patients,increased(0.012_0.051 m/s)in 2 patients and decreased(-0.022 m/s)in 1 patient compared to normal *** in paretic stride lengths ranged from-0.066 to 0.029 *** optimal individualized motor assistance provided by the HWA induces small,positive changes in gait *** indicates that this light-weight hip-assistive exoskeleton can be of value in rehabilitation setting,where multiple training sessions with the device are possible.

关键词： Wearable robotics Exoskeleton Gait Stroke Honda walking assist

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Role of joint aspiration before re-implantation in patients with a cement spacer in place

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World Journal of Orthopedics 2022年第6期13卷 615-621页

作者： Sandra Huguet martíBernaus Lucía Gómez eva Cuchí Alex Soriano Lluís Font-Vizcarra Department of Traumatology and Orthopaedics Hospital Universitari Mútua TerrassaTerrassa 08221Spain Department of Traumatology and Orthopaedics Consorci Sanitari de l’Alt Penedès-GarrafVilafranca del Penedès 08720Spain Department of Traumatology and Orthopaedics Osteoarticular Infections UnitHospital Universitari Mútua TerrassaTerrassa 08221Spain Osteoarticular Infections Unit Hospital Universitari Mútua TerrassaTerrassa 08221Spain Department of microbiology CATLABViladecavalls 08232Spain Department of Infectious Diseases Osteoarticular Infections UnitHospital ClínicBarcelona 08036Spain

BACKGROUND The usefulness of a mandatory joint aspiration before re-implantation in patients with a cement spacer already in place is *** To evaluate the role of culturing synovial fluid obtained by joint aspiration before re-implantation in patients who underwent a two-stage septic *** A retrospective observational study was conducted,including patients that underwent a two-stage septic revision(hip or knee)from 2010 to *** the first stage revision and according to intraoperative culture results,all patients were treated with an antibiotic protocol for 6-8 *** 2 wk without antibiotics,a culture of synovial fluid was *** results of these cultures were recorded and compared with cultures obtained during re-implantation *** Forty-one patients(20 hip and 21 knee spacers)were included in the final *** 39 cases,the culture of synovial fluid was negative,while in the remaining 2 cases(knee spacers)no analysis was possible due to dry *** 5 of the patients,two or more intraoperative cultures taken during the re-implantation surgery were *** We found no evidence to support mandatory joint aspiration before re-implantation in patients with a cement spacer in place.

关键词： Joint aspiration Synovial fluid Two-stage surgery Revision surgery Periprosthetic joint infection

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Tire abrasion particles negatively affect plant growth even at low concentrations and alter soil biogeochemical cycling

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Soil Ecology Letters 2022年第4期4卷 409-415页

作者： eva F.Leifheit Hanna L.Kissener Erik Faltin masahiro Ryo matthias C.Rillig Freie Universitat Berlin Institut fur Biologie14195 BerlinGermany Berlin-Brandenburg Institute of Advanced Biodiversity Research 14195 BerlinGermany Leibniz Centre for Agricultural Landscape Research(ZALF) 15374 MünchebergGermany Brandenburg University of Technology Cottbus-Senftenberg 03046 CottbusGermany

Tire particles(TPs)are a major source of microplastic on land,and considering their chemical composition,they represent a potential hazard for the terrestrial *** studied the effectsof TPs at environmentally relevant concentrations alongawide concentrationgradient(0–160 mg g^(-1))and tested the effects on plant growth,soil pH and the key ecosystem process of litter decomposition and soil *** addition of TPs negatively affected shoot and root growth already at low *** litter decomposition slightly increased with lower additions of TPs but decreased later *** pH increased until a TP concentration of 80 mg g^(-1) and leveled off *** respiration clearly increased with increasing concentration of added *** growth was likely reduced with starting contamination and stopped when contamination reached a certain level in the *** presence of TPs altered a number of biogeochemical soil parameters that can have further effects on plant *** the quantities of yearly produced TPs,their persistence,and toxic potential,we assume that these particles will eventually have a significant impact on terrestrial ecosystems.

关键词： microplastic pollution Tire particles Plant growth Soil respiration Soil pH Litter decomposition

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Debaryomyces hansenii supplementation in low fish meal diets promotes growth,modulates microbiota and enhances intestinal condition in juvenile marine fish

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Journal of Animal Science and Biotechnology 2024年第1期15卷 253-276页

作者： Ignasi Sanahuja Alberto Ruiz Joana P.Firmino Felipe E.Reyes-López Juan B.Ortiz-Delgado eva Vallejos-Vidal Lluis Tort Dariel Tovar-Ramírez Isabel m.Cerezo miguel A.moriñigo Carmen Sarasquete Enric Gisbert Aquaculture Program Institute of Agrifood Research and Technology(IRTA)La Rapita 43540Spain Centro de Biotecnologia Acuicola Universidad de Santiago de ChileSantiagoChile Instituto de Ciencias marinas de Andalucia(ICmAN-CSIC) Avda.Republica Saharaui no 2Campus Universitario Rio San PedroPuerto RealCadiz 11510Spain Nucleo de Investigaciones Aplicadas en Ciencias Veterinarias y Agronomicas Facultad de Medicina Veterinaria y AgronomiaUniversidad de Las AmericasSantiagoChile Department of Cell Biology Physiologyand ImmunologyUniversitat Autonoma de BarcelonaBarcelonaSpain Centro de Investigaciones Biologicas del Noroeste SC CIBNORLa PazMexico Department of microbiology Instituto de Biotecnologia Y Desarrollo Azul(IBYDA)Faculty of SciencesUniversity of Malaga29010 MalagaSpain SCBI Bioinformatic UnitUniversity of Malaga29590 MalagaSpain

Background The development of a sustainable business model with social acceptance,makes necessary to develop new strategies to guarantee the growth,health,and well-being of farmed *** hansenii is a yeast species that can be used as a probiotic in aquaculture due to its capacity to i)promote cell proliferation and differen-tiation,ii)have immunostimulatory effects,iii)modulate gut microbiota,and/or iv)enhance the digestive *** provide inside into the effects of *** on juveniles of gilthead seabream(Sparus aurata)condition,we inte-grated the evaluation of the main key performance indicators coupled with the integrative analysis of the intestine condition,through histological and microbiota state,and its transcriptomic *** After 70 days of a nutritional trial in which a diet with low levels of fishmeal(7%)was supplemented with 1.1%of ***(17.2×10^(5) CFU),an increase of ca.12%in somatic growth was observed together with an improve-ment in feed conversion in fish fed a yeast-supplemented *** terms of intestinal condition,this probiotic modu-lated gut microbiota without affecting the intestine cell organization,whereas an increase in the staining intensity of mucins rich in carboxylated and weakly sulphated glycoconjugates coupled with changes in the affinity for certain lectins were noted in goblet *** in microbiota were characterized by the reduction in abundance of several groups of Proteobacteria,especially those characterized as opportunistic *** microarrays-based transcrip-tomic analysis found 232 differential expressed genes in the anterior-mid intestine of ***,that were mostly related to metabolic,antioxidant,immune,and symbiotic *** Dietary administration of *** enhanced somatic growth and improved feed efficiency param-eters,results that were coupled to an improvement of intestinal condition as histochemical and transcriptomic tools *** probiotic ye

关键词： Debaryomyces hansenii Intestine condition Low fish meal diet microbiota Transcriptomics Yeast probiotic

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Bispecific sigma-1 receptor antagonism and mu-opioid receptor partial agonism:WLB-73502,an analgesic with improved efficacy and safety profile compared to strong opioids

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Acta Pharmaceutica Sinica B 2023年第1期13卷 82-99页

作者： Alba Vidal-Torres Begoña Fernández-Pastor mónica García eva Ayet Anna Cabot Javier Burgueño Xavier monroy Bertrand Aubel Xavier Codony Luz Romero Rosalía Pascual maria Teresa Serafini Gregorio Encina Carmen Almansa Daniel Zamanillo manuel merlos Josémiguel Vela WeLab Barcelona Parc Cientific de BarcelonaBarcelona 08028Spain

Opioids are the most effective painkillers,but their benefit-risk balance often hinder their therapeutic ***-73502 is a dual,bispecific compound that binds sigma-1(S1R)and mu-opioid(mOR)***-73502 is an antagonist at the *** behaved as a partial mOR agonist at the G-protein pathway and produced no/unsignificantβ-arrestin-2 recruitment,thus demonstrating low intrinsic efficacy on mOR at both signalling *** its partial mOR agonism,WLB-73502exerted full antinociceptive efficacy,with potency superior to morphine and similar to oxycodone against nociceptive,inflammatory and osteoarthritis pain,and superior to both morphine and oxycodone against neuropathic ***-73502 crosses the blood-brain barrier and binds brain S1R and mOR to an extent consistent with its antinociceptive *** to morphine and oxycodone,tolerance to its antinociceptive effect did not develop after repeated 4-week ***,contrary to opioid comparators,WLB-73502 did not inhibit gastrointestinal transit or respiratory function in rats at doses inducing full efficacy,and it was devoid of proemetic effect(retching and vomiting)in ferrets at potentially effective ***-73502 benefits from its bivalent S1R antagonist and partial mOR agonist nature to provide an improved antinociceptive and safety profile respect to strong opioid therapy.

关键词： WLB-73502 Sigma-1 receptor mu-opioid receptor Biased agonist Analgesic activity Chronic pain Neuropathic pain Safety

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Comprehensive metabolic Profiling of modified Gegen Qinlian Decoction by Ultra-high-Performance Liquid Chromatography-Diode Array Detection-Q-Exactive-Orbitrap-Electrospray Ionization-mass Spectrometry/mass Spectrometry and Application of High-Performance

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World Journal of Traditional Chinese medicine 2021年第1期7卷 11-32页

作者： Xuehong Nöst eva-maria Pferschy-Wenzig Xiao-Tong Yu min Li Xiao-Lin Tong Rudolf Bauer Department of Pharmacognosy Institute of Pharmaceutical SciencesUniversity of GrazGrazAustria Department of Endocrinology Guang’Anmen HospitalChina Academy of Chinese Medical SciencesBeijingChina Department of Endocrinology Laboratory of Molecular BiologyGuang’Anmen HospitalChina Academy of Chinese Medical SciencesBeijingChina

Objective:Gegen Qinlian decoction(GQD)is a classical traditional Chinese medicine formulation which has been used for almost 2000 *** Guang'anmen Hospital,Beijing,a modified GQD version(m GQD)with seven instead of four herbal ingredients has been applied to treat Type 2 *** control is a crucial prerequisite for the therapeutic application of herbal *** the identification of products derived from classical GQD,the Chinese Pharmacopeia requires the analysis of only three marker *** m GQD is a more complex mixture containing seven herbs and hundreds of constituents,the pharmacopoeia method for GQD is *** and methods:A more comprehensive characterization of the formula's constituents has been developed using ultra-high-performance liquid chromatography-diode array detection(UHPLC-DAD)-Q-Exactive-mass spectrometry(mS)in electrospray ionization positive and negative ***,a new method for the fingerprint analysis of m GQD via high-performance thin-layer chromatography(HPTLC)has been ***:Altogether,91 compounds have been assigned to their originating plants and 84 substances were identified either by comparison with authentic references or with data from the *** HPTLC method is based on the application of two different mobile phases and is able to detect both lipophilic and hydrophilic constituents of m ***:The modified GQD was extensively characterized by UHPLC combined with DAD and Q-Exactive Orbitrap high-resolution mS detection,leading to the assignment and identification of compounds present in the *** addition,a new method for the fingerprint analysis of the m GQD using HPTLC was established,which allows fast and simple identification of the herbal ingredients in the mixture.

关键词： Fructus jujubae HPTLC modified gegen qinlian decoction Radix et rhizoma glycyrrhizae praeparata cum melle Radix puerariae lobatae Radix scutellariae Rhizoma coptidis Rhizoma anemarrhenae Rhizoma zingiberis UHPLC-DAD-Q-Exactive-mS

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