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Bacterial direct-f.d microbials f.il to reduce methane emissions in primiparous lactating dairy cows

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Journal of.Animal Science and Biotechnology 2019年第3期10卷 802-810页

作者： Jeyamalar Jeyanathan Cecile Martin Maguy eugene Anne f.rlay Milka Popova Diego P.Morgavi Universite Clermont Auvergne INRAVetAgro SupUMR 1213 HerbivoresF-63122 Saint-Genes-ChampanelleFrance Laboratory f.r Animal Nutrition and Animal Product Quality Faculty of Bioscience EngineeringGhent UniversityCoupure Links 6539000 GhentBelgium

Direct-f.d microbials(Df.) are considered as a promising technique to improve animal productivity without af.ecting animal health or harming the *** potential of.three bacterial Df. to reduce methane(CH4)emissions,modulate ruminal f.rmentation,milk production and composition of.primiparous dairy cows was examined in this *** previous reports have shown that Df. respond dif.erently to dif.erent diets,two contrasting diets were used in this *** lactating primiparous cows were randomly divided into two groups that were f.d a corn silage-based,high-starch diet(HSD) or a grass silage-based,high-f.ber diet(Hf.).Cows in each dietary group were randomly assigned to f.ur treatments in a 4 × 4 Latin square *** bacterial Df. used were selected f.r their proven CH4-reducing ef.ect in *** included control(without Df.) and 3 Df. treatments: Propionibacterium f.eudenreichii 53-W(2.9 × 10^10 colony f.rming units(Cf.)/cow per day),Lactobacillus pentosus D31(3.6 × 10^11 Cf./cow per day) and Lactobacillus bulgaricus D1(4.6 × 10^10 Cf./cow per day).Each experimental period included 4 weeks of.treatment and 1 week of.wash-out,with measures perf.rmed in the f.urth week of.the treatment *** CH4 emissions were measured during 3 consecutive days using respiration *** samples were collected f.r ruminal f.rmentation parameters and quantitative microbial *** samples were collected f.r composition *** weight of.cows were recorded at the end of.each treatment *** of.diet,no mitigating ef.ect of.Df. was observed on CH4 emissions in dairy *** contrast,Propionibacterium increased CH4 intensity by 27%(g CH4/kg milk) in cows f.d *** was no ef.ect of.Df. on other f.rmentation parameters and on bacterial,archaeal and protozoal ***,the ef.ect of.Df. on milk f.tty acid composition was *** and *** Df. tended to increase body weight gain with ***

关键词： Bacterial direct-f.d microbial Dairy cow Methane Milk f.tty acid

Highly selective/enantioselective Pt-ReOx/C catalyst f.r hydrogenation of.L-malic acid at mild conditions

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Journal of.Energy Chemistry 2018年第3期27卷 903-912页

作者： Bair S.Bal'zhinimaev eugene A.Paukshtis Alexey P.Suknev Nikita V.Makolkin Boreskov Institute of.Catalysis SB RA5 Prospect Akademika Lavrentieva5Novosibirsk 630090Russia

The catalyst preparation strategy was based on a strict introduction sequence of.rhenium and platinum precursors and their strong interaction with carbon support resulted in the f.rmation of.0.5 nm Pt-Re Ox species of.atomic dispersion, where platinum is metallic, while monolayer rhenium is partially oxidized（Re2＋）. The reaction kinetics was studied taking into account the process of.L-malic acid association leading to the f.rmation of.inactive cyclic oligomeric species. High TOf.（ca. 50 h-1）, selectivities（ca. 99%）and stability of.Pt-Re Ox/C catalysts in aqueous-phase hydrogenation of.L-malic acid, which are close to those of.the homogeneous pincer type complexes, were revealed at mild conditions（T = 90–130 ℃）. Taking into account that（i） hydrogenation reaction occurred 2–3 orders of.magnitude f.ster than its racemization and（ii） association of.L-malic acid dominates at low temperatures and in a concentrated solution,special reaction conditions that allow obtaining chemically and optically（ee 〉 99%） pure（S）-3-hydroxy-γ-butyrolactone and（S）-1,2,4-butanetriol were f.und. Basing on HAADf.STEM, EDX, XPS, and kinetic studies, the structure of.active species and basic reaction pathways are proposed.

关键词： L-malic acid Hydrogenation Enantioselectivity Platinum Rhenium Atomic dispersion

Kruppel-like f.ctors and vascular wall homeostasis

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Journal of.Molecular Cell Biology 2017年第5期9卷 352-363页

作者： Yanbo f.n Haocheng Lu Wenying Liang Wenting Hu Jif.ng Zhang Y. eugene Chen Cardiovascular Center Department of Internal Medicine University of Michigan Medical Center Ann Arbor MI 48109 USA

Cardiovascular diseases （CVDs） are major causes of.death worldwide. Identif.cation of.promising targets f.r prevention and treatment of.CVDs is paramount in the cardiovascular f.eld. Numerous transcription f.ctors regulate cellular f.nction through modulation of.spe- cif.c genes and thereby are involved in the physiological and pathophysiologicai processes of.CVDs. Although Kriippel-like f.ctors （KLf.） have a similar protein structure with a conserved zinc f.nger domain, they possess distinct tissue and cell distribution patterns as well as biological f.nctions. In the vascular system, KLf.activities are regulated at both transcriptional and posttranscriptional levels. Growing in vitro, in vivo, and genetic epidemiology studies suggest that specif.c KLf. play important roles in vascular walt biology, which f.rther af.ect vascular diseases. KLf. regulate various f.nctional aspects such as cell growth, dif.erentiation, activa- tion, and development through controlling a whole cluster of.f.nctionally related genes and modulating various signaling pathways in response to pathological conditions. Therapeutic targeting of.selective KLf.f.mily members may be desirable to achieve distinct treatment ef.ects in the context of.various vascular diseases. f.rther elucidation of.the association of.KLf. with human CVDs, their underlying molecular mechanisms, and precise protein structure studies will be essential to def.ne KLf. as promising targets f.r therapeutic interventions in CVDs.

关键词： endothelial cells vascular smooth muscle cells inf.ammation shear stress atherosclerosis vascular injury drugdevelopment

An Approach f.r Searching Insertions in Bacterial Genes Leading to the Phase Shif. of.Triplet Periodicity

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Genomics, Proteomics & Bioinf.rmatics 2011年第4期9卷 158-170页

作者： Maria A. Korotkova Nikolay A. Kudryashov eugene V. Korotkov Centre of.Bioengineering Russian Academy of Sciences National University of.Nuclear Investigations (MIf.)

The concept of.the phase shif. of.triplet periodicity （TP） was used f.r searching potential DNA insertions in genes f.om 17 bacterial genomes. A mathematical algorithm f.r detection of.these insertions has been developed. This approach can detect potential insertions and deletions with lengths that are not multiples of.three bases, especially insertions of.relatively large DNA f.agments （〉 100 bases）. New similarity measure between triplet matrixes was employed to improve the sensitivity f.r detecting the TP phase shif.. Sequences of.17,220 bacterial genes with each consisting of.more than 1,200 bases were analyzed, and the presence of.a TP phase shif. has been shown in -16% of.analysed genes （2,809 genes）, which is about 4 times more than that detected in our previous work. We propose that shif.s of.the TP phase may indicate the shif.s of.reading f.ame in genes af.er insertions of.the DNA f.agments with lengths that are not multiples of.three bases. A relationship between the phase shif.s of.TP and the f.ame shif.s in genes is discussed.

关键词： triplet periodicity insertion gene sequence reading f.ame phase shif. change-point problem

History-dependent percolation on multiplex networks

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National Science Review 2020年第8期7卷 1296-1305页

作者： Ming Li Linyuan Lü Youjin Deng Mao-Bin Hu Hao Wang Matú? Medo H.eugene Stanley Department of.Thermal Science and Energy Engineering University of Science and Technology of China Institute of.f.ndamental and f.ontier Sciences University of Electronic Science and Technology of China Alibaba Research Center f.r Complexity Sciences Hangzhou Normal University Beijing Computational Science Research Center Hef.i National Laboratory f.r Physical Sciences at Microscale Department of Modern Physics and CAS Center for Excellence and Synergetic Innovation Center in Quantum Information and Quantum Physics University of Science and Technology of China Department of.Physics and Center f.r Polymer Studies Boston University

The structure of.interconnected systems and its impact on the system dynamics is a much-studied cross-disciplinary topic. Although various critical phenomena have been f.und in dif.erent models, study of.the connections between dif.erent percolation transitions is still lacking. Here we propose a unif.ed f.amework to study the origins of.the discontinuous transitions of.the percolation process on interacting networks. The model evolves in generations with the result of.the present percolation depending on the previous state, and thus is history-dependent. Both theoretical analysis and Monte Carlo simulations reveal that the nature of.the transition remains the same at f.nite generations but exhibits an abrupt change f.r the inf.nite generation. We use brain f.nctional correlation and morphological similarity data to show that our model also provides a general method to explore the network structure and can contribute to many practical applications, such as detecting the abnormal structures of.human brain networks.

关键词： percolation multiplex networks critical phenomena brain networks

Long-term phosphorus reduction and phytoplankton responses in an urban lake(USA)

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Water Biology and Security 2022年第1期1卷 100-109页

作者： Yuan Grund Yangdong Pan Mark Rosenkranz eugene f.ster Oregon Department of.Environmental Quality OR97232USA Department of.Environmental Science and Management Portland State UniversityPortlandOR97207USA Lake Oswego Corporation Lake OswegoOR97034USA

Eutrophication is one of.the primary f.ctors causing harmf.l cyanobacteria blooms in f.eshwater *** study investigated the long-term changes in water quality and summer phytoplankton assemblages in Oswego Lake,OR,USA,in relation to phosphorus reduction through hypolimnetic aeration and alum *** water quality and phytoplankton assemblages were sampled biweekly during the summers f.om 2001 to *** concentrations of.total phosphorus,soluble reactive phosphorus,and total nitrogen decreased 66%,93%and 31%,respectively,in response to the hypolimnetic aeration and alum treatments since *** phytoplankton assemblages showed a 62%reduction of.cyanobacteria biovolume and a shif. f.om cyanobacteria dominance(2001–2005)to diatom and chlorophyte dominance(2006–2013).Cluster analysis identif.ed f.ur statistically dif.erent groups of.summer phytoplankton assemblages(denoted Groups 1–4).Nonmetric multidimensional scaling analysis indicated that the f.ur groups were associated with dif.erent water quality *** 1 occurred prior to hypolimnetic aeration and was primarily comprised of.cyanobacteria,associated with water conditions of.high nutrients and high primary *** 2,dominated by cyanobacteria and chlorophytes,occurred between hypolimnetic aeration and alum surf.ce *** 3 was dominated by diatoms and occurred af.er alum surf.ce *** 4 included R-strategist phytoplankton that quickly respond to environmental changes and occurred in the years f.llowing alum injection,drawdown,and inf.ow alum *** Group 3 and 4 were associated with reduced nutrients in the *** conclude that these lake management practices had strong ef.ects on both production and community compositions of.phytoplankton,and advocate f.r f.ture studies on large-scale climate impacts on lake ecosystems and to identif. corresponding best management practices.

关键词： Phosphorus reduction Phytoplankton assemblages Urban lake Long-term species data Harmf.l algal blooms Alum application Hypolimnetic aeration

Relevant Visual Semantic Context-Aware Attention-Based Dialog

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Computers, Materials & Continua 2023年第8期76卷 2337-2354页

作者： eugene Tan Boon Hong Yung-Wey Chong Tat-Chee Wan Kok-Lim Alvin Yau National Advanced IPv6 Centre Universiti Sains MalaysiaPenangMalaysia Lee Kong Chian f.culty of.Engineering and Science(LKCf.S) Universiti Tunku Abdul RahmanSungai LongSelangorMalaysia

The existing dataset f.r visual dialog comprises multiple rounds of.questions and a diverse range of.image ***,it f.ces challenges in overcoming visual semantic limitations,particularly in obtaining suf.icient context f.om visual and textual aspects of.*** paper proposes a new visual dialog dataset called Diverse History-Dialog(DS-Dialog)to address the visual semantic limitations f.ced by the existing ***-Dialog groups relevant histories based on their respective Microsof. Common Objects in Context(MSCOCO)image categories and consolidates them f.r each ***,each MSCOCO image category consists of.top relevant histories extracted based on their semantic relationships between the original image caption and historical *** relevant histories are consolidated f.r each image,and DS-Dialog enhances the current dataset by adding new context-aware relevant history to provide more visual semantic context f.r each *** new dataset is generated through several stages,including image semantic f.ature extraction,keyphrase extraction,relevant question extraction,and relevant history dialog *** DS-Dialog dataset contains about 2.6 million question-answer pairs,where 1.3 million pairs correspond to existing VisDial’s question-answer pairs,and the remaining 1.3 million pairs include a maximum of.5 image f.atures f.r each VisDial image,with each image comprising 10-round relevant question-answer ***,a novel adaptive relevant history selection is proposed to resolve missing visual semantic inf.rmation f.r each ***-Dialog is used to benchmark the perf.rmance of.previous visual dialog models and achieves better perf.rmance than previous ***,the proposed DSDialog model achieves an 8% higher mean reciprocal rank(MRR),11% higher R@1%,6% higher R@5%,5% higher R@10%,and 8% higher normalized discounted cumulative gain(NDCG)compared to ***-Dialog also achieves approximately 1 point improvement o

关键词： Visual dialog context-aware relevant history computer vision natural language processing

PUM1 represses CDKN1B translation and contributes to prostate cancer progression

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The Journal of.Biomedical Research 2021年第5期35卷 371-382页

作者： Xin Li Jian Yang Xia Chen Dandan Cao eugene Yujun Xu State Key Laboratory of.Reproductive Medicine Nanjing Medical UniversityNanjingJiangsu 211166China Department of.Urology the Second Affiliated Hospital of Nanjing Medical UniversityNanjingJiangsu 211166China Department of.Neurology Feinberg School of MedicineNorthwestern UniversityChicagoIL 60611USA

Posttranscriptional regulation of.cancer gene expression programs plays a vital role in carcinogenesis;identif.ing the critical regulators of.tumorigenesis and their molecular targets may provide novel strategies f.r cancer diagnosis and *** conserved RNA-binding protein Pumilio-1(PUM1)regulates mouse growth and cell prolif.ration,propelling us to examine its role in *** f.und human PUM1 is highly expressed in a diverse group of.cancer,including prostate cancer;enhanced PUM1 expression is also correlated with reduced survival among prostate cancer *** expression analysis in twenty prostate cancer tissues showed enhanced expression of.PUM1 at mRNA and protein *** of.PUM1 reduced prostate cancer cell prolif.ration and colony f.rmation,and subcutaneous injection of.PUM1 knockdown cells led to reduced tumor *** of.PUM1 in prostate cancer cells consistently elevated cyclin-dependent kinase inhibitor 1B(CDKN1B)protein expression through increased translation but did not impact its mRNA level,while overexpression of.PUM1 reduced CDKN1B protein *** f.nding established a critical role of.PUM1 mediated translational control,particularly the PUM1-CDKN1B axis,in prostate cancer cell growth and *** proposed that PUM1-CDKN1B regulatory axis may represent a novel mechanism f.r the loss of.CDKN1B protein expression in diverse cancers and potential targets f.r therapeutics development.

关键词： PUM1,prostate cancer posttranscriptional regulation CDKN1B

A decomposition of.light’s spin angular momentum density

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Light(Science & Applications) 2024年第8期13卷 1578-1589页

作者： Alex J.Vernon Sebastian Golat Claire Rigouzzo eugene A.Lim f.ancisco J.Rodríguez-f.rtuño Department of.Physics King’s College LondonStrandLondon WC2R 2LSUK London Centre f.r Nanotechnology LondonUK

Light carries intrinsic spin angular momentum(SAM)when the electric or magnetic f.eld vector rotates over time.A f.miliar vector equation calculates the direction of.light’s SAM density using the right-hand rule with ref.rence to the electric and magnetic polarisation *** Maxwell’s equations,this vector equation can be decomposed into a sum of.two distinct terms,akin to the well-known Poynting vector decomposition into orbital and spin *** present the f.rst general study of.this spin decomposition,showing that the two terms,which we call canonical and Poynting spin,are chiral analogies to the canonical and spin momenta of.light in its interaction with *** canonical momentum,canonical spin is directly *** canonical and Poynting spin incorporate spatial variation of.the electric and magnetic f.elds and are inf.uenced by optical *** decomposition allows us to show that a linearly polarised vortex beam,which has no total SAM,can nevertheless exert longitudinal chiral pressure due to equal and opposite canonical and Poynting spins.

关键词： canonical momentum angular