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Detection of pathogenic bacteria by magneto-immunoassays:a review

The Journal of Biomedical Research 2021年第4期35卷 277-283页

作者： eugen gheorghiu International Centre of Biodynamics Bucharest 060101Romania.

Magnetic particle-based immunoassays are widely used in microbiology-related assays for both microbial capture,separation,analysis,and *** facilitating sample operation,the implementation of micro-to-nanometer scale magnetic beads as a solid support potentially shortens the incubation time(for magnetic immuno capture)from several hours to less than an *** technologies based on magnetic beads offer a rapid,effective and inexpensive way to separate and concentrate the target analytes prior to ***-immuno separation uses magnetic particles coated with specific antibodies to capture target microorganisms,bear the corresponding antigens,and subsequently separate them from the sample matrix in a magnetic *** method has been proven effective in separating various types of pathogenic bacteria from environmental water samples and in eliminating background *** particles are often used to capture target cells(pathogenic bacteria)from *** most commercially available assays,the actual identification and quantitation of the captured cells is then performed by classical microbiological *** review highlights the most sensitive analytic methods(i.e.,long-range surface plasmon resonance and electrochemical impedance spectroscopy)to detect magnetically tagged bacteria in conjunction with magnetic actuation.

关键词： magneto-immuno separation long-range surface plasmon resonance electrochemical impedance spectroscopy periodic magnetic actuation

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High-resolution impedance mapping using electrically activated quantitative phase imaging

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Light(Science & Applications) 2021年第2期10卷 186-196页

作者： Cristina Polonschii Mihaela gheorghiu Sorin David Szilveszter Gáspár Sorin Melinte Hassaan Majeed Mikhail E.Kandel Gabriel Popescu eugen gheorghiu International Centre of Biodynamics 060101 BucharestRomania Institute of Information and Communication Technologies Electronics and Applied MathematicsUniversite Catholique de Louvain1348 Louvain-la-NeuveBelgium Quantitative Light Imaging Laboratory Beckman Institute for Advanced Science and TechnologyUniversity of Illinois at Urbana-ChampaignUrbanaIL 61801USA

Retrieving electrical impedance maps at the nanoscale rapidly via nondestructive inspection with a high signal-to-noise ratio is an unmet need,likely to impact various applications from biomedicine to energy *** this study,we develop a multimodal functional imaging instrument that is characterized by the dual capability of impedance mapping and phase quantitation,high spatial resolution,and low temporal *** achieve this,we advance a quantitative phase imaging system,referred to as epi-magnified image spatial spectrum microscopy combined with electrical actuation,to provide complementary maps of the optical path and electrical *** demonstrate our system with high-resolution maps of optical path differences and electrical impedance variations that can distinguish nanosized,semi-transparent,structured coatings involving two materials with relatively similar electrical *** map heterogeneous interfaces corresponding to an indium tin oxide layer exposed by holes with diameters as small as~550 nm in a titanium(dioxide)over-layer deposited on a glass *** show that electrical modulation during the phase imaging of a macro-electrode is decisive for retrieving electrical impedance distributions with submicron spatial resolution-and beyond the limitations of electrode-based technologies(surface or scanning technologies).The findings,which are substantiated by a theoretical model that fits the experimental data very well enable achieving electro-optical maps with high spatial and temporal *** virtues and limitations of the novel optoelectrochemical method that provides grounds for a wider range of electrically modulated optical methods for measuring the electric field locally are critically discussed.

关键词： impedance resolution phase

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