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Sperm function, mitochondrial activity and in vivo fertility are associated to their mitochondrial DNA content in pigs

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Journal of Animal Science and Biotechnology 2024年第4期15卷 1550-1558页

作者： Marc Llavanera Yentel Mateo‑Otero estel Vinolas‑Vergés Sergi Bonet Marc Yeste Unit of Cell Biology Department of BiologyFaculty of SciencesUniversity of GironaGirona ES‑17003Spain Biotechnology of Animal and Human Reproduction(TechnoSperm) Institute of Food and Agricultural TechnologyUniversity of GironaGirona ES‑17003Spain Catalan Institution for Research and Advanced Studies(ICREA) Barcelona ES‑08010Spain

Background Despite their low abundance in sperm, mitochondria have diverse functions in this cell type, includ-ing energy production, signalling and calcium regulation. In humans, sperm mitochondrial DNA content(mtDNAc) has been reported to be negatively linked to sperm function and fertility. Yet, the association between mtDNAc and sperm function in livestock remains unexplored. For this reason, this study aimed to shed some light on the link between mtDNAc and sperm function and fertilising potential in pigs. A qPCR method for mtDNAc quantification was optimised for pig sperm, and the association of this parameter with sperm motility, kinematics, mitochondrial activity, and fertility was subsequently *** First, the q PCR method was found to be sensitive and efficient for mtDNAc quantification in pig sperm. By using this technique, mtDNAc was observed to be associated to sperm motility, mitochondrial activity and in vivo, but not in vitro, fertility outcomes. Specifically, sperm with low mtDNAc were seen to exhibit greater motility but decreased mitochondrial activity and intracellular reactive oxygen species. Interestingly, samples with lower mtD-NAc showed higher conception and farrowing rates, but similar in vitro fertilisation rates and embryo development, when compared to those with greater *** These findings enrich our comprehension of the association of mtDNAc with sperm biology, and lay the foundation for future research into employing this parameter as a molecular predictor for sperm function and fer-tility in livestock.

关键词： Fertility Mitochondria Motility mtDNA copy number qPCR Sperm

Chromatin condensation but not DNA integrity of pig sperm is greater in the sperm-rich fraction

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Journal of Animal Science and Biotechnology 2024年第1期15卷 171-181页

作者： estel Viñolas-Vergés Jordi Ribas-Maynou Isabel Barranco Camila Peres Rubio Sergi Bonet Jordi Roca Marc Yeste Unit of Cell Biology Department of BiologyFaculty of SciencesUniversity of GironaGironaSpain Biotechnology of Animal and Human Reproduction(TechnoSperm) Institute of Food and Agricultural TechnologyUniversity of GironaGironaSpain Department of Medicine and Animal Surgery Faculty of Veterinary ScienceUniversity of MurciaMurciaSpain Catalan Institution for Research and Advanced Studies(ICREA) S08010 BarcelonaSpain

Background Protamination and condensation of sperm chromatin as well as DNA integrity play an essential role during fertilization and embryo *** some mammals,like pigs,ejaculates are emitted in three separate fractions:pre-sperm,sperm-rich(SRF)and post sperm-rich(PSRF).These fractions are known to vary in volume,sperm concentration and quality,as well as in the origin and composition of seminal plasma(SP),with differences being also observed within the SRF ***,whether disparities in the DNA integrity and chromatin condensation and pro-tamination of their sperm exist has not been *** This study determined chromatin protamination(Chromomycin A3 test,CMA_(3)),condensation(Dibromobi-mane test,DBB),and DNA integrity(Comet assay)in the pig sperm contained in the first 10 m L of the SRF(SRF-P1),the remaining portion of the sperm-rich fraction(SRF-P2),and the post sperm-rich fraction(PSRF).While chromatin protamination was found to be similar between the different ejaculate fractions(P>0.05),chromatin condensation was seen to be greater in SRF-P1 and SRF-P2 than in the PSRF(P=0.018 and P=0.004,respectively).Regarding DNA integrity,no differences between fractions were observed(P>0.05).As the SRF-P1 has the highest sperm concentra-tion and ejaculate fractions are known to differ in antioxidant composition,the oxidative stress index(OSi)in SP,calcu-lated as total oxidant activity divided by total antioxidant capacity,was tested and confirmed to be higher in the SRF-P1 than in SRF-P2 and PSRF(0.42±0.06 vs.0.23±0.09 and 0.08±0.00,respectively;P<0.01);this index,in addition,was observed to be correlated to the sperm concentration of each fraction(Rs=0.973;P<0.001).Conclusion While sperm DNA integrity was not found to differ between ejaculate fractions,SRF-P1 and SRF-P2 were observed to exhibit greater chromatin condensation than the *** could be related to the OSi of each fraction.

关键词： Chromatin Condensation DNA integrity Ejaculate fractions Pig Protamination Sperm

Understanding Chinese Qigong in the context of Western culture

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Journal of Acupuncture and Tuina Science 2018年第5期16卷 315-318页

作者： estel Vilar Universitat Oberta de Catalunya Gestió Cultural Barcelona Spain

It tries to present various perspectives on the value that Qigong brings to the Western world,responding to medical,creative and spiritual needs,and proposes a cultural approach to its dissemination that can encompass all its *** the current growth of China's soft power and the implementation of the Belt and Road Initiative,there is a clear opportunity for the development of traditional Chinese medicine (TCM) and Qigong abroad that shall bear fruit in the near future,gradually shifting the role of China in the world stage:from a manufactured products supplier to a major influence in culture,lifestyle and thought.

关键词： Traditional Chinese Medicine Physical and Breathing Exercises Qigong Exercise Cultural Globalization Tai Ji The Belt and Road Initiative

Determination of double-and single-stranded DNA breaks in bovine sperm is predictive of their fertilizing capacity

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Journal of Animal Science and Biotechnology 2023年第1期14卷 134-151页

作者： Jordi Ribas‑Maynou Ariadna Delgado‑Bermúdez Yentel Mateo‑Otero estel Vinolas Carlos O.Hidalgo WSteven Ward Marc Yeste Biotechnology of Animal and Human Reproduction(TechnoSperm) Faculty of SciencesInstitute of Food and Agricultural TechnologyUniversity of GironaC/Maria Aurèlia Campany69ES‑17003 GironaSpain. Unit of Cell Biology Department of BiologyFaculty of SciencesUniversity of GironaES‑17003 GironaSpain. Institute for Biogenesis Research Department of AnatomyBiochemistry&PhysiologyJohn A.Burns School of MedicineUniversity of Hawaii at ManoaHonoluluHI 96822USA. Department of Animal Selection and Reproduction The Regional Agri-Food Research and Development Service of Asturias(SERIDA)ES‑33394 GijónSpain. Catalan Institution for Research and Advanced Studies(ICREA) ES‑08010 BarcelonaSpain.

Background:The analysis of chromatin integrity has become an important determinant of sperm *** frozenthawed bovine sperm,neither the sequence of post-thaw injury events nor the dynamics of different types of sperm DNA breaks are well *** aim of the present work was to describe such sperm degradation aftermath focusing on DNA damage dynamics,and to assess if this parameter can predict pregnancy rates in ***:A total of 75 cryopreserved ejaculates from 25 Holstein bulls were evaluated at two post-thawing periods(0-2 h and 2-4 h),analyzing global and double-stranded DNA damage through alkaline and neutral Comet assays,chromatin deprotamination and decondensation,sperm motility,viability,acrosomal status,and intracellular levels of total ROS,superoxides and *** of 59,605 females was conducted using sperm from the same bulls,thus obtaining the non-return to estrus rates after 90 d(NRR).Results showed an increased rate of double-stranded breaks in the first period(0-2 h:1.29±1.01%/h vs.2-4 h:0.13±1.37%/h;P0.05).Finally,whereas no correlations between bull fertility and any dynamic parameter were found,there were correlations between the NRR and the basal percentage of highly-damaged sperm assessed with the alkaline Comet(Rs=-0.563,P=0.003),between NRR and basal progressive motility(Rs=0.511,P=0.009),and between NRR and sperm with high ROS at 4 h post-thaw(Rs=0.564,P=0.003).Conclusion:The statistically significant correlations found between intracellular ROS,sperm viability,sperm motility,DNA damage

关键词： Cattle Chromatin Comet test DNA damage Fertility Sperm Sperm quality

Global Burden of Cardiovascular Disease

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Cardiovascular Innovations and Applications 2016年第B9期1卷 369-377页

作者： Christopher estel C.Richard Conti Division of Cardiovascular Diseases Department of MedicineUniversity of Florida GainesvilleFL 32608USA

Cardiovascular disease(CVD)is the number one cause of mortality world-wide and places a high medical and socioeconomic burden on developing *** understanding of CVD and its evolution over the last 100 years has altered *** for the increased rate of CVD in the developing world include rapid urbanization and the demographic shift known as the modern epidemiologic *** case for intervention is based on both major human and economic impacts of *** has been estimated that cost-effective interventions in developing countries with a high burden of CVD could result in a projected 24 million lives *** reduction in CVD mortality could reduce economic costs by$8 *** to intervention include:1)cardiovascular health promotion and CVD prevention and 2)action plans advocated by the World Health Organization.

关键词： global health cardiology cardiovascular disease urbanization

MgO,碱度,磨矿粒度及膨润土添加剂对球团矿生产的影响

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国外钢铁 1995年第10期20卷 1-4页

作者： estel.,D 刘金琨

本文介绍了ＭｇＯ、二元碱度、磨矿粒度及膨润土添加剂对球团矿冶金性能影响的最新成果，并由小规模试验确定生产用于直接还原的高质量球团矿所需的工艺条件。

关键词：球团矿添加剂氧化镁碱度磨矿膨润土

制革工业产生的毛废弃物与脱墨污泥及城市污水处理系统污泥的共堆肥

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北京皮革（中外鞋讯）（下） 2010年第6期 80-84页

作者： Raquel Barrena estel.la Pagans Adriana Artola Fel i citas V a zquez Antoni S a nchez 龚英（译）巴赛罗纳自治大学四川大学制革清洁技术国家工程实验室成都610065

由于采用保毛脱毛技术，制革工业产生的毛废弃物量是逐年增加的，这使得制革人员还要处理另一种固体废弃物。对于毛的利用已经提出了多种潜在的方案，其中，由于毛含氮量高，因此，将毛废弃物转变为农用肥料将是最有前景的应用方法之一... 详细信息

由于采用保毛脱毛技术，制革工业产生的毛废弃物量是逐年增加的，这使得制革人员还要处理另一种固体废弃物。对于毛的利用已经提出了多种潜在的方案，其中，由于毛含氮量高，因此，将毛废弃物转变为农用肥料将是最有前景的应用方法之一。可通过堆肥进一步提高毛的农用价值。本文研究了牛皮加工中脱毛工序产生毛废弃物的堆肥。研究结果表明：毛废弃物不能单独堆肥，也不可与脱墨污泥这种作为化学补充的共同基质进行堆肥。当毛废弃物与城市污水处理系统原污泥以1：1、1：2和1：4质量比在实验室和试难工厂进行共堆肥时，能得到好的结果。在这些情况中，堆肥工艺结束时，均能得到更稳定的产品。试验工厂堆肥可通过测定取样时工艺温度（SRIT）和37℃（SRI37）时的静志呼吸指数而有效监控。特别指出，SRIT值对生物活性的变化更敏感。相对而言，呼吸商数（RQ）值并不是与工艺过程的进行同步的。

关键词：生物活性指数 C/N比共堆肥脱墨污泥毛废弃物,城市污水处理系统原污泥脱毛残渣