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Chemical and microbiological characterization of tinctures and microcapsules loaded with Brazilian red propolis extract

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Journal of Pharmaceutical Analysis 2017年第5期7卷 280-287页

作者： erika Tayse da Cruz Almeida Maria Cristina Delgado da Silva José Marcos dos santos Oliveira Regianne Umeko Kamiya Rodolfo Elleson dos santos Arruda Danilo Abreu Vieira Valdemir da Costa Silva Pierre Barnabé Escodro Irinaldo Diniz Basílio-Júnior Ticiano Gomes do Nascimento Laboratório de Controle de Qualidade e Análise de Fármacos Medicamentos e AlimentosCurso de FarmáciaEscola de Enfermagem e FarmáciaUniversidade Federal de AlagoasCampus A.C.SimõesBR 104 Norte-Km 97MaceióAL.CEP:57072-970Brazil Laboratório de Controle de Qualidade Microbiológico de Alimentos Faculdade de NutriçãoUniversidade Federal de AlagoasCampus A.C.SimõesBR 104 Norte/Km 97MaceióAL.CEP:57072-970Brazil

The aim of this study was to characterize tinctures and microcapsules loaded with an ethanol extract of red propolis through chemical, physicochemical and microbiological assays in order to establish quality control tools for nutraceutical preparations of red propolis. The markers(isoflavonoids, chalcones, pterocarpans,flavones, phenolic acids, terpenes and guttiferones) present in the tinctures A and B were identified and confirmed using LC/ESI/FTMS/Orbitrap. Four compositions(A, B, C and D) were prepared to contain B tincture of the red propolis with some pharmaceutical excipients and submitted to two drying processes, i. ***-drying and freeze-drying to obtain microcapsules loaded with the red propolis extract. The tinctures and microcapsules of the red propolis were submitted to the total flavonoid content and antioxidant activity *** antibacterial activity and minimum inhibitory concentration(MIC) were tested using Staphylococcus aureus ATCC 25293 and Pseudomonas aeruginosa ATCC 27853 strains. The tinctures and microcapsules presented high flavonoid quantities from 20.50 to 40.79 mg/100 mg of the microcapsules. The antioxidant activity and IC50 were determined for the tinctures A and B(IC50: 6.95 μg/m L and 7.48 μg/m L), the spray-dried microcapsules(IC50: 8.89–15.63 μg/m L) and the freeze-dried microcapsules(IC50: 11.83–23.36 μg/m L). The tinctures and microcapsules were proved to be bioactive against gram-positive and gram-negative bacteria with inhibition halos superior to 10 mm at concentration of 200 μg/m L and MIC values of 135.87–271.74 μg/m L using gram-positive strain and 271.74–543.48 μg/m L using gram-negative strain. The tinctures and microcapsules of the red propolis have a potential application for nutraceutical products.

关键词： Red propolis Tinctures Microcapsules Isoflavonoids LC/ESI/FTMS/Orbitrap Antioxidant activity Antibacterial activity

Canine Cutaneous Mast Cell Tumor: Biologic Behavior and Its Correlation with Prognostic Indicators

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Open Journal of Veterinary Medicine 2012年第4期2卷 255-261页

作者： Sabrina dos santos Costa Poggiani erika Maria Terra Rafael Torres Neto Mirela Tinucci Costa Renée Laufer Amorim Department of Veterinary Clinics Veterinary Medicine and Animal Science School Universidade Estadual Paulista Júlio de Mesquita Filho Botucatu Brazil Department of Veterinary Medicine Pioneer Union of Social Integration Brasilia Brazil

Cutaneous mast cell tumor (MCT) shows a variable biological behavior in dogs and may present either as solitary masse that can be treated and cured with surgical removal or as a systemic metastatic and fatal disease. Histological grade, KIT pattern and proliferative index are typically prognostic factors in MCTs. In the present study, we have investigated correlation between clinical data (breed, age, gender, tumour location, tumor size, time before surgery, number of tumours, occurrence of metastasis and recurrence), cellular proliferation (Ki-67, mitotic index), intratumoural microvessel density (IMVD) and apoptotic index with the histological grade and KIT pattern. 28 tumors, from 20 dogs with cutaneous MCT were evaluated. There was association between histological grade, IMVD, tumor ulceration and number of tumors. A significant increase of Ki-67 expression and mitotic index was observed in MCTs with cytoplasmic KIT staining pattern. Patnaik histological grade system was related to mitotic index. Histological grade in canine cutaneous MCT should not be assessed as the only prognostic factor, but associated with KIT pattern, IMDV, cellular proliferation, presence of tumour ulceration, number of tumours, recurrences and metastases.

关键词： Dog Mast Cell Tumor Prognosis Biologic Behavior

Microbial enzymatic stoichiometry and the acquisition of C, N,and P in soils under different land-use types in Braziliansemiarid

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Soil Ecology Letters 2023年第3期5卷 101-108页

作者： erika Valente de Medeiros Érica de Oliveira Silva Gustavo Pereira Duda Mario Andrade Lira Junior Uemeson Josédos santos Claude Hammecker Diogo Paes da Costa Fabio Fernando Araujo Arthur Prudêncio de Araujo Pereira Lucas William Mendes Ademir Sergio Ferreira Araujo Laboratory of Microbiology and Enzymology-LEMA Federal University of Agreste PernambucoGaranhuns 55292-270Brazil Federal Rural University of Pernambuco Rua Dom Manuel de Medeiross/nDois Irmãos52171-900RecifeBrazil Soil-Agrosystem-Hydrosystem Interaction Lab-LISAH Place Pierre Viala234060 MontpellierFrance Universidade do Oeste Paulista Presidente PrudenteSPBrazil Departamento de Ciência Do Solo Universidade Federal Do CearáFortalezaCEBrazil Center for Nuclear Energy in Agriculture University of Sao PauloPiracicabaSPBrazil Soil Quality Lab Agricultural Science CenterFederal University of PiauíTeresinaPI 60049-550Brazil

This study hypothesized that different land-use affect the microbial enzymatic stoichiometry and C-,N-,and P-acquisition in Brazilian semiarid ***,the enzymesβ-glucosidase(C-acquiring enzyme),urease(N-acquiring enzyme),and acid phosphatase(P-acquiring enzyme)were assessed in soil samples collected at 0−5 and 5−10 cm depth from a tropical dry forest,a protected area with Angico,a protected area with Ipê,scrub area,and an agricultural area with *** values of C-,N-,and P-acquiring enzymes were used to calculate the enzymatic C:N,C:P,and N:P *** values of C:P and N:P ratios were higher at 0−5 cm depth,while no significant variation,between soil depth,was observed for C:N *** values of C-and N-acquiring enzymes were higher at 0−5 cm in tropical dry forest areas and Angico forest,*** all land use types,the values of vectors L and A were higher than 1°and 45°,*** study showed that both land-use and soil depth influence the enzymatic stoichiometry,showing higher values of C-and N-acquiring enzymes in native and protected forests at soil surface.

关键词： C:N:P stoichiometry tropical forests soil depth Luvisols

Micropropagation of Eucalyptus grandis×*** AEC 224 clone

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Journal of Forestry Research 2017年第1期28卷 29-39页

作者： Cassiana de Oliveira Juliana Degenhardt-Goldbach Gisela Manuela de Franca Bettencourt erika Amano Luziane Franciscon Marguerite Quoirin Department of Plant Science Federal University of Parana1540Rua dos FuneionariosCuritibaParanaBrazil Embrapa-Forest 111Estrada da RibeiraColomboParanaBrazil Department of Technology Federal University of Parana210avenida CelFrancisco Heraiclito dos SantosCuritibaParanaBrazil Department of Botany Federal University of Parana210avenida CelFrancisco Heraiclito dos SantosCuritibaParanaBrazil

Genetic transformation systems require protocols that allow regenerating transgenic plants from transformed tissues. This study aimed to establish a protocol for indirect organogenesis in leaf explants of a Eucalyptus grandis x E. urophylla AEC 224 clone. During callogenesis stage, several concentrations of NAA and then NAA or 2,4-D combined with TDZ were tested in JADS culture medium for 30 days, followed by subculture of the explants in the regeneration medium, containing 5.0 μM BA and 0.5 μM NAA for another 30 days. In these media, the explant oxidation rate was high （95 %）. Thus, in order to reduce oxidation, different ;culture media were compared： WPM, MS, JADS and modified QL, followed by explant transfer onto regeneration medium. The highest percentage of regeneration and the lowest oxidation rate were achieved on WPM medium. Then, NAA and 2,4-D were tested in combination with TDZ and also TDZ and BA combined with NAA in WPM medium. The most efficient culture media in terms of shoot regeneration were WPM supplemented with 0.25 μM TDZ and 0.1μM NAA during 30 days for callus induction and then with 5.0 μM BA and 0.5 μM NAA for another 30 days. This protocol yielded a regeneration rate of 43 %, with a low oxidation of tissues. A rooting experiment was conducted using half strength MS medium and comparing three concentrations of IBA （2.46, 4.90 and 7.35μM）. The highest rooting percentage （35 %） was obtained on medium containing 2.46μM IBA. Once the shoots were rooted, acclimatization in a greenhouse was not challenging and plant survival reached 100 %.

关键词： Callogenesis Eucalyptus urograndis Organogenesis WPM culture medium

Sexual Diversity and Homophobia in Health Care Services: Perceptions of Homosexual and Bisexual Population in the Cross-Cultural Theory

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Open Journal of Nursing 2016年第6期6卷 470-482页

作者： Grayce Alencar Albuquerque Glauberto da Silva Quirino Francisco Winter dos santos Figueiredo Laércio da Silva Paiva Luiz Carlos de Abreu Vitor Engrácia Valenti Vânia Barbosa do Nascimento erika da Silva Maciel Fernando Rodrigues Peixoto Quaresma Fernando Adami Regional University of Cariri Ceará Brazil School of Medicine of ABC S&atildeo Paulo Brazil Paulista State University Campus of Marilia S&atildeo Paulo Brazil University Center Lutheran of Palmas CEULP-ULBRA Tocantins Brazil

The provision of care by health professionals seems to be related to cultural patterns in which these workers are inserted. The relationship between culture and care becomes a challenge for the provision of health care to homosexual and bisexual population. Thus, this study aimed to identify the perception of Gays, Lesbians, Bisexuals and Transgenders in relation to the care received from health professionals, in the light of Cross-Cultural Theory. This is a qualitative, ethnographic study, conducted with 30 members of sexual minorities in Juazeiro do Norte, Brazil. The data analysis lead to the elaboration of five categories based on of the Theory of Cultural Care Diversity and Universality. The participants emphasized the constant violation of their rights to universal and equitable access to the National Health System, stating that they have suffered from prejudice and exclusion, as result of a heteronormative culture. They highlighted that homophobia and dehumanization of care have a strong relation to sexual orientation. The transvestites are the most prone to humiliation and pejorative attitudes of professionals and such condition is a barrier to accessing services.

关键词： Health Care Health Disparities Discrimination Homophobia Theory

Elevated Human Chorionic Gonadotrophin Due to Pancreatic Cancer

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Open Journal of Anesthesiology 2012年第4期2卷 178-182页

作者： Asha Manohar erika Werner Jamie Murphy Anesthesia and Critical Care Medicine The Johns Hopkins Hospital Baltimore MD USA Department of Gynecology and Obstetrics The Johns Hopkins Hospital Baltimore MD USA

Preoperative pregnancy testing is common practice among anesthesiologists. Little is re-ported in anesthesia literature on causes other than pregnancy for elevated human chorionic go-nadotrophin (hCG) levels. Among these causes is hCG secretion by tumors. This is a report of a female with pancreatic cancer that presented for a Whipple procedure, who on routine pregnancy testing was found to have an elevated hCG level. This unexpected result led to significant delay and undue stress for the patient. After determining pregnancy was unlikely and multiple poorly differentiated metastatic masses were discovered, it was decided that the most likely cause of her hCG levels was cancer. Multiple cancers have been reported to secrete hCG, and more specifically β hCG [1]. Studies of elevated hCG in pancreatic cancer patients report an incidence of 40% - 56% [2,3]. Serum β hCG testing done prior to the date of surgery (dos) in cancer pa- tients that are pre- or perimenopausal should be considered as it would allow time for a more definitive pregnancy evaluation to be performed.

关键词： Pregnancy Test Cancer β hCG

Circulating leptin and its muscle gene expression in Nellore cattle with divergent feed efficiency

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Journal of Animal Science and Biotechnology 2018年第1期9卷 228-232页

作者： Lúcio Flávio Macedo Mota Cristina Moreira Bonafé Pamela Almeida Alexandre Miguel Henrique Santana Francisco JoséNovais erika Toriyama Aldrin Vieira Pires Saulo da Luz Silva Paulo Roberto Leme JoséBento Sterman Ferraz Heidge Fukumasu Departmento de Zootecnia Universidade Federal dos Vales do Jequitinhonha e MucuriDiamantinaMG 39100 000Brazil Faculdade de Ciencias Agrarias e Veterinarias Universidade Estadual Paulista JaboticabalSP 14884 900Brazil Departamento de Medicina Veterinaria Faculdade de Zootecnia e Encjenharia de AlimentosUniversidade de Sao PauloAM.Duque de Caxias Norte n225Pirassununcja 13635 900SPBrazil Departmento de Zootecnia e Desenvolvimento Acjrossocioambiental Sustentavel Faculdade de VeterinariaUniversidade Federal FluminenseNiteroiRJ 24230 340Brazil Departamento de Zootecnia Faculdade de Zootecnia e Engenharia de AlimentosUniversidade de Sao PauloPirassununga 13635-900SPBrazil

Background： Leptin has a strong relation to important traits in animal production, such as carcass composition,feed intake, and reproduction. It is mainly produced by adipose cells and acts predominantly in the *** this study, circulating leptin and its gene expression in muscle were evaluated in two groups of young Nellore bulls with divergent feed efficiency. Individual dry matter intake（DMI） and average daily gain（ADG） of 98 Nellore bulls were evaluated in feedlot for 70 d to determinate the residual feed intake（RFI） and select 20 animals for the high feed efficient（LRFI） and 20 for the low feed efficient（HRFI） groups. Blood samples were collected on d 56 and at slaughter（80 d） to determine circulating plasma leptin. Samples of Longissimus dorsi were taken at slaughter for leptin gene expression ***： DMI and RFI were different between groups and LRFI animals showed less back fat and rump fat thickness,as well as less pelvic and kidney fat weight. Circulating leptin increased over time in all animals. Plasma leptin was greater in LRFI on 56 d and at slaughter（P = 0.0049）. Gene expression of leptin were greater in LRFI animals（P = 0.0022） in accordance with the plasma levels. The animals of the LRFI group were leaner, ate less, and had more circulating leptin and its gene ***： These findings demonstrated that leptin plays its physiological role in young Nellore bulls, probably controlling food intake because feed efficient animals have more leptin and lower residual feed intake.

关键词： Beef cattle Energy homeostasis Fat depositon Residual feed intake

Turmeric and Vitamin E on the Performance and Quality of Eggs from White Layers

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Agricultural Sciences 2019年第6期10卷 720-726页

作者： Christine Laganá erika S. P. Braga Saldanha José Roberto Sartori Elisabeth Gonzales Renato Luis Luciano Greice Zanatta Vitor Barbosa Fascina Agência Paulista de Tecnologia dos Agronegócios Polo Regional do Leste Paulista and UPD Brotas Secretariat of Agriculture and Supply to the State of S&#227 o Paulo (SAA) Monte Alegre do Sul Brazil Faculty of Veterinary Medicine and Zootechnics State University of S&#227 o Paulo Botucatu Campus Botucatu Brazil Advanced Center for Poultry Agribusiness Technological Research of the Biological Institute (SAA) Descalvado Brazil

A 112-day experiment was conducted to evaluate turmeric and vitamin E levels on the productive performance and quality of hen eggs. A total of 432 20-week-old white layers were used, distributed in an entirely randomized design, in a 4 × 3 factor scheme (turmeric extract levels and vitamin E levels), with 6 repetitions of 6 birds per cage. The treatments consisted of four levels of turmeric extract inclusion (0;0.1;0.2 and 0.3) and three levels of vitamin E inclusion (0;50 and 100 IU/kg). Egg quality was evaluated at each 28-day cycle through the variables specific gravity, yolk percentage, albumen percentage, shell percentage and thickness, Haugh unit, yolk index and staining, and production performance: weight, egg production and mass, feed intake, feed conversion (kg/kg;kg/dz), and mortality. The addition of vitamin E alone worsened feed intake, egg weight and feed conversion of layers. Turmeric added to 100 IU of Vit E improved egg mass indices, Haugh Unit and yolk index and intensified yolk staining. The amount of turmeric used in this experiment was not sufficient to improve the performance or egg quality of the birds.

关键词： Plant Extracts Productive Performance Phytogenic Additives

Laying Egg Production Supplemented with Antioxidants: An Economical View

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Agricultural Sciences 2019年第5期10卷 665-670页

作者： Christine Laganá erika Salgado Politi Braga Saldanha José Roberto Sartori Elisabeth Gonzales Patrícia Helena Nogueira Turco Renato Luis Luciano Greice Zanatta Adelina Azevedo Agência Paulista de Tecnologia dos Agronegócios Polo Regional do Leste Paulista and UPD Brotas Secretariat of Agriculture and Supply to the State of S&#227 o Paulo (SAA) Monte Alegre do Sul Brazil Faculty of Veterinary Medicine and Zootechnics State University of S&#227 o Paulo Botucatu Campus Botucatu Brazil Advanced Center for Poultry Agribusiness Technological Research of the Biological Institute (SAA) Descalvado Brazil

A 112-day experiment was conducted to economically evaluate the production of eggs from commercial layers submitted to diets supplemented with levels of plant extracts (dried turmeric powder) added or not to vitamin E. A total of 432 20-week-old white layers were used, distributed in an entirely randomized design, in a 4 × 3 factor scheme (turmeric extract levels and vitamin E levels), with 6 repetitions of 6 birds per cage. The treatments consisted of four levels of turmeric extract inclusion (0;0.1%;0.2% and 0.3%) and three levels of vitamin E inclusion (0;50 and 100 IU/kg). The results calculated at work were favorable to the activity, and the items that most influenced the expenses in the effective production cost were: poultry, feed and labor, respectively. The best economic response in egg production was obtained by layers that consumed 100 IU/kg vitamin E and 0.2% turmeric;because the leveling point represents that the activity equals the revenues with the costs. The best profitability index was found in treatment that contained 50 IU/kg vitamin E and 0.1% turmeric com (16.78%).

关键词： Plant Extracts Phytogenic Additives Turmeric Vitamin E