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The cold-induced fa.tor CBF3 media.es root stem cell a.tivity,regenera.ion,a.d developmenta. responses to cold

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Pla.t Communica.ions 2023年第6期4卷 197-211页

作者： Pa.lo perez-Ga.cia.Ornella.Puccia.iello a.va.o sanchez-Corrionero Ja.ier Ca.rera.Cristina.del Ba.rio Jua. Ca.los del Pozo Ma.ia.o Pera.es Krzysztof Wa.nik Miguel a.Moreno-Risueno Centro de Biotecnología. Genómica.de Pla.ta.(Universida. Politécnica.de Ma.rid(UPM)–Instituto Na.iona. de Investiga.ióny Tecnología.a.ra.ia.y a.imenta.ia.CSIC(INIa.CSIC)) MadridSpain

Pla.t growth a.d development involve the specifica.ion a.d regenera.ion of stem cell niches(SCNs).a.though pla.ts a.e exposed to dispa.a.e environmenta. conditions,how environmenta. cues a.fect developmenta. progra.s a.d stem cells is not well *** stem cells a.e a.commoda.ed in meristems in SCNs a.ound the quiescent center(QC),which ma.nta.ns their *** a.combina.ion of genetics a.d confoca. microscopy to tra.e morphologica. defects a.d correla.e them with cha.ges in gene expression a.d protein levels,we show tha. the cold-induced tra.scription fa.tor(TF)C-REPEa. BINDING Fa.TOR 3(CBF3),which ha. previously been a.socia.ed with cold a.clima.ion,regula.es root development,stem cell a.tivity,a.d ***3 is integra.ed into the SHORT-ROOT(SHR)regula.ory network,forming a.feedba.k loop tha. ma.nta.ns SHR ***3 is prima.ily expressed in the root endodermis,wherea. the CBF3 protein is loca.ized to other meristema.ic tissues,including root *** of cbf3-1 using a.wild-type CBF3 gene a.d a.CBF3 fusion with reduced mobility show tha. CBF3 movement ca.a.ity is required for SCN pa.terning a.d regula.es root ***,cold induces CBF3,a.fecting QC ***,exposure to modera.e cold a.ound 10℃–12℃promotes root regenera.ion a.d QC respecifica.ion in a.CBF3-dependent ma.ner during the recupera.ion *** contra.t,CBF3 does not a.pea. to regula.e stem cell surviva.,which ha. been a.socia.ed with recupera.ion from more a.ute cold(-4℃).We propose a.role for CBF3 in media.ing the molecula. interrela.ionships a.ong the cold response,stem cell a.tivity,a.d development.

关键词： stem cells regenera.ion orga. pa.terning protein movement cell fa.e low tempera.ure

Modelling potentia. distribution of a.pine ba.k beetle in Mexica. tempera.e forests using foreca.t da.a.a.d spa.ia. a.a.ysis tools

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Journa. of Forestry Resea.ch 2020年第2期31卷 649-659页

作者： a.tonio Gonza.ez-Herna.dez Rene Mora.es-Villa.a.a.Ma.tin enrique Romero-sanchez Brenda.Isla.-Trejo Ra.iro perez-Mira.da Conserva.ion a.d Enha.cement of Forest Ecosystems National Institute of ForestryAgriculture and Livestock ResearchProgreso 504010 Coyoacan Mexico CityMexico Geogra.hy Depa.tment The National Autonomous University of MexicoUniversidad 300004010 Coyoacan Mexico CityMexico

a.cura.e a.d relia.le predictions of pest species distributions in forest ecosystems a.e urgently needed by forest ma.a.ers to develop ma.a.ement pla.s a.d monitor new a.ea. of potentia. ***-only species distribution models a.e commonly used in these *** ma.imum entropy a.gorithm(Ma.Ent)ha. ga.ned popula.ity for modelling species ***,Ma.Ent wa. used to model the spa.ia. distribution of the Mexica. pine ba.k beetle(Dendroctonus mexica.us)in a.da.ly fa.hion by using foreca.t da.a.from the Wea.her Resea.ch a.d Foreca.ting *** study a.med to exploit freely a.a.la.le geogra.hic a.d environmenta. da.a.a.d softwa.e a.d thus provide a.pa.hwa. to overcome the la.k of costly da.a.a.d technica. guida.ce tha. a.e a.cha.lenge to implementing na.iona. monitoring a.d ma.a.ement stra.egies in developing *** results showed overa.l a.reement va.ues between 60 a.d 87%.The results of this resea.ch ca. be used for *** monitoring a.d ma.a.ement a.d ma. a.d a. a.model to monitor simila. species.

关键词： Spa.ia. a.a.ysis Dendroctonus mexica.us Geoda.a.a.es Ma.Ent Forest modelling

Intra.xa.iner repea.a.ility a.d a.reement in stereoa.uity mea.urements ma.e in young a.ults

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Interna.iona. Journa. of Ophtha.mology(English edition) 2015年第2期8卷 374-381页

作者： Bea.riz a.tona.a.a.Ba.rio Isa.el sanchez enrique Gonza.ez Gua.a.upe Gonza.ez Depa.tment of Optics II (Optometry a.d Vision) Faculty of Optics and Optometry Complutense University of Madrid

a.M: To determine the repea.a.ility a.d a.reement of stereoa.uity mea.urements ma.e using some of the most widely used clinica. tests: Frisby, TNO, Ra.dot a.d ***: Stereoa.uity wa. mea.ured in two different sessions sepa.a.ed by a.time interva. of a. lea.t 24 h but no longer tha. 1wk in 74 subjects of mea. a.e 20.6y using the four methods. The study pa.ticipa.ts were divided into two groups: subjects with norma. binocula. vision a.d subjects with a.norma. binocula. ***: Best repea.a.ility wa. shown by the Frisby a.d Titmus [coefficient of repea.a.ility(COR): ±13 a.d±12s a.c respectively] in the subjects with norma. binocula. vision though a.clea. ceiling effect wa. *** the subjects with a.norma. binocula. vision, best repea.a.ility wa. shown by the Frisby(COR: ±69s a.c)a.d Ra.dot(COR: ±72s a.c). In both groups, the TNO test showed poorest a.reement with the other ***:Therepea.a.ilityofstereoa.uitymea.ures wa. low in subjects with poor binocula. vision yet fa.rly good in subjects with norma. binocula. vision with the exception of the TNO test. The reduced a.reement detected between the tests indica.es they ca.not be used intercha.gea.ly.

关键词： stereoa.uity repea.a.ility a.reement tereopsis

Sa.S-CoV-2:a.r pollution highly correla.ed to the increa.e in *** ca.e of Gua.a.a.a.a.Ja.isco,Mexico

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Infectious Disea.e Modelling 2023年第2期8卷 445-457页

作者： Eliza.eth Torres-a.guia.o Itzel sanchez-Lopez a.geles Ga.duno-Robles Jorge Da.id Riva.-Ca.rillo Edga. a.fonso Rivera.Leon Sergio sanchez-Enríquez Luis Ferna.do Ornela.-Herna.dez Ferna.do Za.ueta.Leon-Quintero Edua.do Na.ciso Sa.a.a. Leon-Quintero Guillermo enrique Jua.ez-Lopez Ferna.do a.tonio sanchez-Zubieta.Ma.ia.a.Ochoa.Bru a.ra.a. Zepeda.Moreno Onkogenetik/Mexica.a.de Investiga.ion y Biotectogía.S.a.de C.V GuadalajaraJaliscoMexico Unida. de Biología.Molecula. Investigacion y DiagnosticoHospital San JavierGuadalajaraJaliscoMexico Depa.ta.ento de Fisiología Centro Universitario de Ciencias de la SaludUniversidad de GuadalajaraGuadalajaraJaliscoMexico Depa.ta.ento de Biología.Molecula. y Genomica Centro Universitario de Ciencias de la SaludUniversidad de GuadalajaraGuadalajaraJaliscoMexico Depa.ta.ento de Clínica. Centro Universitario de los AltosUniversidad de GuadalajaraTepatitlan de MorelosJaliscoMexico Depa.ta.ento de Clínica. Medica. Centro Universitario de Ciencias de la SaludUniversidad de GuadalajaraGuadalajaraJaliscoMexico Servicio de Hema.o-Oncología.Pedia.rica Hospital Civil de Guadalajara“Dr.Juan I.Menchaca”GuadalajaraJaliscoMexico Hospita. Civil de Gua.a.a.a.a.Fra. a.tonio a.ca.de” GuadalajaraJaliscoMexico

Objectives:To determine whether a.r pollution or cha.ges in Sa.S-CoV-2 linea.es lea. to a. increa.e in ***:Descriptive sta.istics were used to ca.cula.e ra.es of infection(2020-2021).RT ePCR wa. used to compa.e vira. loa.s from October 2020 to Februa.y *** sequencing(NGS)(n=92)wa. used to exa.ine a.d phylogenetica.ly ma. Sa.S-CoV-2 linea.es.a.correla.ive“a.r pollution/tempera.ure”index(I)wa. developed using regression ***_(2.5),PM_(10),O_(3),NO_(2),SO_(2),a.d CO concentra.ions were a.a.yzed a.d compa.ed to the ***:The morta.ity ra.e during the la.t yea. wa.~32%.Rela.ive Sa.S-CoV-2 vira. loa.s increa.ed in December 2020 a.d Ja.ua.y *** revea.ed tha. a.proxima.ely 80%of Sa.S-CoV-2 lina.es were B.1.243(33.7%),B1.1.222(11.2%),B.1.1(9%),B.1(7%),B.1.1.159(7%),a.d B.1.2(7%).Two periods were a.a.yzed,the prehigh-a.d high-morta.ity periods a.d no significa.t linea.e differences or new linea.es were *** correla.ions of a.r pollution/tempera.ure index va.ues with morta.ity were found for IPM_(2.5) a.d IPM_(10).INO_(2).ISO_(2),a.d ICO but not for O_(3).Using ICO,we developed a.model to predict morta.ity with a. estima.ed va.ia.ion of~±5 dea.hs per da..

关键词： a.r pollution COVID-19 Gua.a.a.a.a Mexico Sa.S-CoV-2 Sa.S-CoV-2 linea.es

a.a.1 inhibition promotes cell recovery From a.optosis

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Protein & Cell 2015年第11期6卷 833-843页

作者： a.na.Gorta. Monica.Sa.cho La.ra.Mondra.on a.gel Messeguer enrique perez-Pa.a.Ma. Orza.z La.ora.ory of Peptide a.d Protein Chemistry Centro de Investigaci6n Principe Felipe 46012 Valencia Spain Depa.tment of Chemica. a.d Biomolecula. Na.otechnology Instituto Quimica Avanzada de CataluSa (CSIC) 08034 Barcelona Spain Instituto de Biomedicina.de Va.encia IBV-CSIC 46010 Valencia Spain

The protein a.optoUc protea.e a.tiva.ing fa.tor I （a.a.l） is the centra. component of the a.optosome, a.multiprotein complex tha. a.tiva.es proca.pa.e-9 a.ter cytochrome c relea.e from the mitochondria.in the intrinsic pa.hwa. of a.optosis. We ha.e developed a.vita. method tha. a.lows fluorescence-a.tiva.ed cell sorting of cells a. different sta.es of the a.optotic pa.hwa. a.d demonstra.ed tha. upon pha.ma.ologica. inhibition of a.a.l, cells recover from doxorubicinor hypoxia.induced ea.ly a.optosis to norma. hea.thy cell. Inhibiting a.a.l not only prevents proca.pa.e-9 a.tiva.ion but dela.s ma.sive mitochondria. da.a.e a.lowing cell recovery.

关键词： a.a.1 a.a.1 inhibitors a.optosis,a.optosome a.topha.y cell recovery

a.tioxida.t a.d immunomodula.ory effects of Viusid in pa.ients with chronic hepa.itis C

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World Journa. of Ga.troenterology 2010年第21期16卷 2638-2647页

作者： Edua.do Vila. Gomez Ya.ina.Ma.tinez perez Hector Vega.sanchez Gretel Riveron Forment enrique a.us Soler Luis Ca.za.illa.Bertot a.i Ya.ells Ga.cia.Ma.ia.del Rosa.io a.reu Va.quez Licet Gonza.ez Fa.ia. Depa.tment of Hepa.ology National Institute of GastroenterologyHavana 10400Cuba Depa.tment of Ga.troenterology National Institute of GastroenterologyHavana 10400Cuba Depa.tment of Immunology National Institute of GastroenterologyHavana 10400Cuba Depa.tment of Oxida.ive Stress National Genetic CenterHavana 10400Cuba Depa.tment of Biosta.istics National Institute of GastroenterologyHavana 10400Cuba Depa.tment of Pa.hology Institute of GastroenterologyHavana 10400Cuba

a.M:To investiga.e the effica.y of Viusid,a.nutritiona. supplement,a. a. a.tioxida.t a.d a. immunomodula.or in pa.ients with chronic hepa.itis ***:Sixty pa.ients with chronic hepa.itis C who were non-responders to sta.da.d a.tivira. trea.ment were ra.domly a.signed to receive Viusid(3 sa.hets da.ly,n=30) or pla.ebo(n=30) for 24 *** prima.y outcome wa. the cha.ge in serum ma.ondia.dehyde a.d 4-hydroxya.kena.s(lipid peroxida.ion products).Seconda.y outcomes were cha.ges in serum tumor necrosis fa.tor-α(TNF-α),interferon-γ(IFN-γ) a.d interleukin-10(IL-10).RESULTS:Sta.istica.ly significa.t reductions in serum 4-hydroxya.kena.s a.d ma.ondia.dehyde levels were observed in both groups in compa.ison with pretrea.ment va.ues,but the pa.ients who received Viusid showed a.more ma.ked reduction a. compa.ed with the control group(P=0.001).TNF-α levels significa.tly increa.ed from 6.9 to 16.2 pg/mL(Pa.ients who received pla.ebo in compa.ison with a.most uncha.ged levels,from 6.6 to 7.1 pg/mL(P=0.26),in the pa.ients trea.ed with Viusid(P=0.001).In a.dition,IL-10 levels were ma.kedly increa.ed in the pa.ients trea.ed with Viusid(from 2.6 to 8.3 pg/mL,P=0.04) in contra.t to the pa.ients a.signed to pla.ebo(from 2.8 to 4.1 pg/mL,P=0.09)(P=0.01).Likewise,the a.ministra.ion of Viusid ma.kedly increa.ed mea. IFN-γ levels from 1.92 to 2.89 pg/mL(Pa.ison with a.reduction in mea. levels from 1.80 to 1.68 pg/mL(P=0.70) in the pla.ebo group(Pa.ministra.ion wa. well ***:Our results indica.e tha. trea.ment with Viusid lea.s to a.nota.le improvement of oxida.ive stress a.d immunologica. pa.a.eters in pa.ients with chronic hepa.itis C.

关键词： a.tioxida.t thera.y Chronic hepa.itis C Cytokines Immunomodula.ory thera.y Nutritiona. supplement Oxida.ive stress

Modelling fuel loa.s of understorey vegeta.ion a.d forest floor components in pine sta.ds in NW Spa.n

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Forest Ecosystems 2022年第6期9卷 775-789页

作者： Jose a.Vega.Stefa.o a.ella.o-perez Jua. Ga.riel a.va.ez-Gonza.ez Cristina.Ferna.dez enrique Jimenez Pedro Cuina. Jose Ma.ía.Ferna.dez-a.onso Da.iel J.Vega.Nieva.Ferna.do Ca.tedo-Dora.o Cecilia.a.onso-Rego Teresa.Fontúrbel a.a.Da.ía.Ruiz-Gonza.ez Centro de Investiga.ion Foresta. de Louriza. PO Box 12736080PontevedraSpain Unida. de Gestion a.bienta. y Foresta. Sostenible(UXa.ORES) Departamento de Ingeniería AgroforestalEscuela Politecnica Superior de IngenieríaUniversidad de Santiago de CompostelaCampus Universitario s/n27002LugoSpain Fa.ulta. de Ciencia. Foresta.es Universidad Juarez del Estado de DurangoRío Papaloapan y Blvd.Durango s/nCol.Valle del Sur34120DurangoMexico Depa.ta.ento de Ingeniería.y Ciencia. a.ra.ia. Universidad de LeonCampus de Ponferrada24401PonferradaSpain

In this study,310 destructively sa.pled plots were used to develop two equa.ion systems for the three ma.n pine species in NW Spa.n(***;*** a.d ***):one for estima.ing loa.s of understorey fuel components by size a.d condition(live a.d dea.)a.d a.other one for forest floor *** systems of equa.ions were simulta.eously fitted for estima.ing fuel loa.s using overstorey,understorey a.d forest floor va.ia.les a. *** systems of equa.ions included both the effect of pine species a.d the effect of understorey compositions domina.ed by ferns-bra.bles or by woody species,due to their obvious structura. a.d physiologica. *** genera.,the goodness-of-fit sta.istics indica.ed tha. the estima.es were rea.ona.ly robust a.d a.cura.e for a.l of the fuel *** best results were obta.ned for tota. understorey vegeta.ion,tota. forest floor a.d ra. humus fuel loa.s,with more tha. 76%of the observed va.ia.ility expla.ned,wherea. the poorest results were obta.ned for coa.se fuel loa.s of understory vegeta.ion with a.53%of observed va.ia.ility *** reduce the overa.l costs a.socia.ed with the field inventories necessa.y for opera.iona. use of the models,the a.ditive systems were fitted a.a.n using only overstorey va.ia.les a. potentia. *** rela.ionships for fine(a.d tota. understorey vegeta.ion a.d tota. forest floor fuel loa.s were obta.ned,indica.ing the complexity of the forest overstorey-understorey a.d overstorey-forest floor ***,these models expla.ned a.ound 52%of the observed ***,equa.ions estima.ing the tota. understorey vegeta.ion a.d the tota. forest floor fuel loa.s ba.ed only on ca.opy cover were *** models expla.ned only 26%-32%of the observed va.ia.ility;however,their ma.n a.va.ta.e is tha. a.though understorey vegeta.ion in forested la.dsca.es is la.gely invisible to remote sensing,ca.opy cover ca. be estima.ed with modera.e a.cura.y,a.lowin

关键词： a.ea.ba.ed a.proa.h a.ditive equa.ion systems Live a.d dea. fine fuel Pinus pina.ter Pinus ra.ia.a Pinus sylvestris

a.trocytes a.d retrogra.e degenera.ion of nigrostria.a. dopa.inergic neurons in Pa.kinson’s disea.e:removing a.ona. debris

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Tra.sla.iona. Neurodegenera.ion 2021年第4期10卷 554-572页

作者： Ingrid Mora.es Rica.do Puerta.-a.enda.o a.berto sanchez a.ria. perez-Ba.reto Cla.a.Rodriguez-Sa.a.e Ma.uel Rodriguez La.ora.ory of Neurobiology a.d Experimenta. Neurology Department of Basic Medical SciencesFaculty of MedicineLa Laguna UniversityLa LagunaTenerifeCanary IslandsSpain Center for Networked Biomedica. Resea.ch on Neurodegenera.ive Disea.es(CIBERNED) MadridSpain

Objective:The dopa.inergic nigrostria.a. neurons(Da.cells)in hea.thy people present a.slow degenera.ion with a.ing,which produces cellula. debris throughout *** 2%-5%of people present ra.id cell degenera.ion of more tha. 50%of Da.cells,which produces Pa.kinson's disea.e(PD).Neuroinfla.ma.ion a.celera.es the cell degenera.ion a.d ma. be critica. for the tra.sition between the slow physiologica. a.d the ra.id pa.hologica. degenera.ion of Da.cells,pa.ticula.ly when it a.tiva.es microglia. cells of the media. forebra.n bundle nea. dopa.inergic *** syna.tic debris produced by Da.cell degenera.ion ma. trigger the pa.kinsonia. neuroinfla.ma.ion,this study investiga.ed the remova. of a.ona. debris produced by retrogra.e degenera.ion of Da.cells,pa.ing pa.ticula. a.tention to the rela.ive roles of a.trocytes a.d ***:Ra.s a.d mice were injected in the la.era. ventricles with 6-hydroxydopa.ine,inducing a.degenera.ion of dopa.inergic syna.ses in the stria.um which wa. not a.compa.ied by non-selective tissue da.a.e,microgliosis or *** possible retrogra.e degenera.ion of dopa.inergic a.ons,a.d the production a.d meta.oliza.ion of Da.cell debris were studied with immunohistochemica. methods a.d a.a.yzed in confoca. a.d electron microscopy ***:The selective degenera.ion of dopa.inergic syna.ses in the stria.um wa. followed by a.retrogra.e degenera.ion of dopa.inergic a.ons whose debris wa. found within spheroids of the media. forebra.n *** spheroids reta.ned mitochondria.a.d most(e.g.,tyrosine hydroxyla.e,the dopa.ine tra.sporter protein,a.d a.yloid precursor protein)but not a.l(e.g.,α-synuclein)proteins of the degenera.ing dopa.inergic *** showed initia.(a.topha.osomes)but not la.e(lysosomes)components of a.topha.y(incomplete a.topha.y).These spheroids were penetra.ed by a.trocytic processes of the media. forebra.n bundle,which provided the lysosomes needed to continue the degra.a.ion of dopa.inergic debri

关键词： a.trocyte Pha.ocytosis Pa.kinson's disea.e Dying-ba.k degenera.ion Media. forebra.n bundle Spheroid

Impa.red immune rea.tion a.d increa.ed la.ta.e a.d C-rea.tive protein for ea.ly prediction of severe morbidity a.d pa.crea.ic fistula.a.ter pa.crea.oduodenectomy

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Hepa.obilia.y & Pa.crea.ic Disea.es Interna.iona. 2020年第1期19卷 58-67页

作者： Ma.io Rodriguez-Lopez Fra.cisco J.Tejero-Pintor Ma.tin Ba.lon-Cua.ra.o a.terio Ba.rera.Rebollo Ba.ta.a. perez-Sa.orido Da.id Pa.heco-sanchez Genera. a.d Digestive Surgery Depa.tment Rio-Hortega University Hospital

Ba.kground: Prediction of complica.ions a.ter pa.crea.oduodenectomy (PD) rema.ns of interest. Blood pa.a.eters a.d bioma.kers during rst a.d second postopera.ive da.s (POD1, POD2) ma. be ea.ly indi- ca.ors of complica.ions. Methods: This ca.e-control study included 50 pa.ients. Ba.eline, POD1 a.d POD2 va.ues of leukocytes, neutrophils, lymphocytes, pla.elets, hemoglobin, C-rea.tive protein (CRP), proca.citonin a.d a.teria. la.ta.e were compa.ed between individua.s presenting Cla.ien ≥ III morbidity, pa.crea.ic stula.(PF) or clinica.ly releva.t PF (CRPF) a.d those without these morbidities. Common va.ia.les rea.hing signi ca.ce were further a.a.yzed in order to ca.cula.e a.predictive score. Results: Severe morbidity, PF a.d CRPF ra.es were 28.0%, 26.0% a.d 14.0%, respectively. Pa.ients with severe morbidity ha. lower leukocytes on POD2 (P=0.04). Pa.ients with PF presented higher CRP on POD2 (P=0.001), higher la.ta.e on POD1 (P=0.007) a.d POD2 (P=0.008), a.d lower lymphocytes on POD1 (P=0.007) a.d POD2 (P=0.008). Pa.ients with CRPF ha. lower leukocytes a.d neutrophils on POD1 (P =0.048, P =0.038), lower lymphocytes on POD1 (P =0.001) a.d POD2 (P =0.003), a.d higher CRP on POD2 (P =0.001). Ba.eline pa.a.eters a.d proca.citonin obta.ned no sta.istica. a.socia.ions. Score wa. de ned a.cording to lymphocytes on POD1 a.d CRP on POD2 ≥ 250 mg/L a.loca.ing pa.ients in 3 risk ca.egories. PF a.d CRPF ra.es were sta.istica.ly higher a. risk ca.egory increa.ed (Pa.ing cha.a.teristic curves a.d Hosmer Lemeshow tests showed a.good a.cura.y. Conclusions: Impa.red immunologica. rea.tion during ea.ly postopera.ive period (lower leukocytes a.d, pa.ticula.ly, lymphocytes) in response to surgica. a.gression would fa.or complica.ions a.ter PD. Likewise, a.idosis (higher a.teria. la.ta.e) could beha.e a. risk fa.tor of PF. a. eleva.ed CRP on POD2 is a.so a. ea.ly bioma.ker of PF. Our novel score ba.ed on postopera.ive lymphocyte count a.d CRP seems relia.le

关键词： Pa.crea.oduodenectomy Morbidity Pa.crea.ic fistula Bioma.ker Prediction Score