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sperm function, mitochondrial activity and in vivo fertility are associated to their mitochondrial DNA content in pigs

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Journal of Animal science and Biotechnology 2024年第4期15卷 1550-1558页

作者： Marc Llavanera Yentel Mateo‑Otero Estel Vinolas‑vergés sergi Bonet Marc Yeste Unit of Cell Biology Department of BiologyFaculty of SciencesUniversity of GironaGirona ES‑17003Spain Biotechnology of Animal and Human Reproduction(Technosperm) Institute of Food and Agricultural TechnologyUniversity of GironaGirona ES‑17003Spain Catalan Institution for Research and Advanced studies(ICREA) Barcelona ES‑08010Spain

Background Despite their low abundance in sperm, mitochondria have diverse functions in this cell type, includ-ing energy production, signalling and calcium regulation. In humans, sperm mitochondrial DNA content(mtDNAc) has been reported to be negatively linked to sperm function and fertility. Yet, the association between mtDNAc and sperm function in livestock remains unexplored. For this reason, this study aimed to shed some light on the link between mtDNAc and sperm function and fertilising potential in pigs. A qPCR method for mtDNAc quantification was optimised for pig sperm, and the association of this parameter with sperm motility, kinematics, mitochondrial activity, and fertility was subsequently *** First, the q PCR method was found to be sensitive and efficient for mtDNAc quantification in pig sperm. By using this technique, mtDNAc was observed to be associated to sperm motility, mitochondrial activity and in vivo, but not in vitro, fertility outcomes. specifically, sperm with low mtDNAc were seen to exhibit greater motility but decreased mitochondrial activity and intracellular reactive oxygen species. Interestingly, samples with lower mtD-NAc showed higher conception and farrowing rates, but similar in vitro fertilisation rates and embryo development, when compared to those with greater *** These findings enrich our comprehension of the association of mtDNAc with sperm biology, and lay the foundation for future research into employing this parameter as a molecular predictor for sperm function and fer-tility in livestock.

关键词： Fertility Mitochondria Motility mtDNA copy number qPCR sperm

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Chromatin condensation but not DNA integrity of pig sperm is greater in the sperm-rich fraction

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Journal of Animal science and Biotechnology 2024年第1期15卷 171-181页

作者： Estel Viñolas-vergés Jordi Ribas-Maynou Isabel Barranco Camila Peres Rubio sergi Bonet Jordi Roca Marc Yeste Unit of Cell Biology Department of BiologyFaculty of SciencesUniversity of GironaGironaSpain Biotechnology of Animal and Human Reproduction(Technosperm) Institute of Food and Agricultural TechnologyUniversity of GironaGironaSpain Department of Medicine and Animal surgery Faculty of Veterinary ScienceUniversity of MurciaMurciaSpain Catalan Institution for Research and Advanced studies(ICREA) S08010 BarcelonaSpain

Background Protamination and condensation of sperm chromatin as well as DNA integrity play an essential role during fertilization and embryo *** some mammals,like pigs,ejaculates are emitted in three separate fractions:pre-sperm,sperm-rich(sRF)and post sperm-rich(PsRF).These fractions are known to vary in volume,sperm concentration and quality,as well as in the origin and composition of seminal plasma(sP),with differences being also observed within the sRF ***,whether disparities in the DNA integrity and chromatin condensation and pro-tamination of their sperm exist has not been *** This study determined chromatin protamination(Chromomycin A3 test,CMA_(3)),condensation(Dibromobi-mane test,DBB),and DNA integrity(Comet assay)in the pig sperm contained in the first 10 m L of the sRF(sRF-P1),the remaining portion of the sperm-rich fraction(sRF-P2),and the post sperm-rich fraction(PsRF).While chromatin protamination was found to be similar between the different ejaculate fractions(P>0.05),chromatin condensation was seen to be greater in sRF-P1 and sRF-P2 than in the PsRF(P=0.018 and P=0.004,respectively).Regarding DNA integrity,no differences between fractions were observed(P>0.05).As the sRF-P1 has the highest sperm concentra-tion and ejaculate fractions are known to differ in antioxidant composition,the oxidative stress index(Osi)in sP,calcu-lated as total oxidant activity divided by total antioxidant capacity,was tested and confirmed to be higher in the sRF-P1 than in sRF-P2 and PsRF(0.42±0.06 vs.0.23±0.09 and 0.08±0.00,respectively;Ps index,in addition,was observed to be correlated to the sperm concentration of each fraction(Rs=0.973;Psion While sperm DNA integrity was not found to differ between ejaculate fractions,sRF-P1 and sRF-P2 were observed to exhibit greater chromatin condensation than the *** could be related to the Osi of each fraction.

关键词： Chromatin Condensation DNA integrity Ejaculate fractions Pig Protamination sperm

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Investigating the inflow into a granular bed using a locally resolved method

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Particuology 2024年第2期85卷 89-101页

作者： Maximilian Brommer Maik scharnowski enric Illana Mahiques siegmar Wirtz Viktor scherer Ruhr University Bochum Institute of Energy Plant TechnologyUniversitätsstraβe 15044780BochumGermany

Discrete Element Method-Computational Fluid Dynamics(DEM/CFD)simulations of industrial-scale granular systems employ spatial averaging(porous media approach)for the fluid-particle interaction in the whole domain,which can lead to poor accuracy,for instance at flow inlets,as local particle bulk morphology is not *** paper presents an approach where the interstitial flow in crucial areas with large gradients can be resolved locally in an otherwise unresolved domain,so that a mixed resolved-unresolved method is *** a generic example to show the feasibility and performance of the new approach,the inflow of ambient air into a flat-bottom hopper through a narrow orifice is *** an experimental setup,the vertical profile of the pressure decay through the inlet and across the packing is chosen for com-parison with respective *** obtained with the conventional porous media method and the locally resolved approach are compared to these experiments for varying volume flow rates and for two different particle *** of different size as well as dodecahedrons are *** is found that although averaging methods already provide good approximations,the locally resolved method can improve the result especially when conventional drag laws are not applicable due to wall effects or if large velocity gradients exist.

关键词： DEM/CFD Packed bed Pressure drop

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A reduced model for particle calcination for use in DEM/CFD simulations

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Particuology 2024年第10期93卷 316-327页

作者： Lucas Mieg Torben Bergold enric Illana Mahiques Viktor scherer Martin Mönnigmann Automatic Control and system Theory Ruhr University BochumGermany Energy Plant Technology Ruhr University BochumGermany

We treat the accurate simulation of the calcination reaction in particles,where the particles are large and,thus,the inner-particle processes must be *** these processes need to be described with coupled partial differential equations(PDEs)that must be solved numerically,the computation times for a single particle are too high for use in simulations that involve many *** of this type arise when the Discrete Element Method(DEM)is combined with Computational Fluid Dynamics(CFD)to investigate industrial systems such as quicklime production in lime shaft *** show that,based on proper orthogonal decomposition and Galerkin projection,reduced models can be derived for single particles that provide the same spatial and temporal resolution as the original PDE models at a considerably reduced computational *** the finite volume particle models with the reduced models results in an overall reduction of the reactor simulation time by about 40%for the sample system treated here.

关键词： Calcination Reactive bulk Model reduction Discrete element method Galerkin projection

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shape-changing particles for locally resolved particle geometry in DEM simulations

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Particuology 2024年第6期89卷 185-190页

作者： enric Illana Klidi Qyteti Maik scharnowski Maximilian Brömmer siegmar Wirtz Viktor scherer Institute of Energy Plant Technology Ruhr-University BochumBochumGermany

Taking into account the complex shape of particles in discrete element method (DEM) simulations of large-scale granular systems is computationally demanding due to the time-consuming contact detection algorithms for polyhedral particles. In this short communication, a novel approach that locally resolves the particle shapes where needed and uses a simplified representation elsewhere, to accelerate simulations without compromising accuracy, is presented. For this purpose, a method employing a smooth transition of the particle shape representation from analytical spheres to shape-resolving polyhedra is introduced in DEM. The feasibility and correct implementation of this approach are demonstrated through simulations of hopper discharge involving spherical and dodecahedral particles from a flat bottom silo or shaft kiln. The model capabilities, in terms of accuracy as well as reduction in computational effort, are quantified for a moving bed with continuous outflow.

关键词： Polyhedron size change shape change Locally resolved DEM

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Convective drying of wood chips:Accelerating coupled DEM-CFD simulations with parametrized reduced single particle models

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Particuology 2024年第1期84卷 158-167页

作者： Lucas Reineking Jonas Fischer Ali Mjalled enric Illana siegmar Wirtz Viktor scherer Martin Monnigmann Automatic Control and system Theory Ruhr-Universität-BochumUniversitätstraße 150Bochum44801Germany Energy Plant Technology Ruhr-Universität-BochumUniversitätstraße 150Bochum44801Germany

The simulation of industry-scale reactive bulks is challenging due to the complex interaction between fluid and *** particles in the bulk and their interaction with the fluid flow can be described by combined Discrete Element Method-Computational Fluid Dynamics(DEM-CFD)***,the computational cost of the Finite Volume(FV)methods deployed in these models can become prohibi-tively expensive,especially for high inner-particle *** particle Reduced Models(RMs)can be used to achieve both fast and accurate descriptions of the processes in each *** an example of bulk systems comprising heat and mass transfer,we compared FV and RM simulations for the drying of wood chips in a bulk reactor.A manifold-based nonlinear interpolation was applied to resolve changing boundary conditions for the *** simulations showed that RMs provide accurate values for the thermodynamic state variables of the ***,the time required for the bulk simulation was reduced by 67%with the *** is evident that simulations with high inner-particle resolution can be accelerated by RMs if manifold-based nonlinear interpolation is used to address changing boundary conditions.

关键词： Reduced model Reactive bulk Discrete element method Manifold-based interpolation Drying of porous mediaDEM-CFD

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Comparing two IBM implementations for the simulation of uniform packed beds

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Particuology 2024年第3期86卷 1-12页

作者： Christian Gorges Maximilian Brommer Christin Velten siegmar Wirtz enric Illana Mahiques Viktor scherer Katharina Zahringer Berend van Wachem Mechanical Process Engineering Otto-von-Guericke-Universitat MagdeburgUniversitatsplatz 239106MagdeburgGermany Department of Energy Plant Technology Ruhr-Universitat BochumUniversitatsstraBe 15044780BochumGermany Laboratory of Fluid Dynamics and Technical Flows Otto-von-Guericke-Universitat MagdeburgUniversitatsplatz 239106MagdeburgGermany

Nowadays,the design of fixed packed bed reactors still relies on empirical correlations,which,especially for small tube to particle diameter ratios,are mostly too inaccurate because of the presence of wall ***,the simulation of fixed packed bed reactors plays an important role to predict and control the flow and process parameters in such,nowadays and in the *** of its straightforward applicability to non-uniform packings with particles of arbitrary shapes,the immersed boundary method(IBM)has advantages over other numerical methods and is used more and more *** paper compares two approaches of IBMs for the simulation of fixed bed reactors with spherical shaped *** classic,smooth approach is compared to the straightforward to implement blocked-off method for velocity fields above the fixed bed for particle Reynolds numbers of 300 and *** from experimental inline PIV-measurements of the reactor to be simulated serve as a basis for *** good agreement with the experiment is found for both simulation methodologies with higher resolutions,considering the more stable flow at a particle Reynolds number of *** in the different IBM approaches occurred for the more unsteady flow at a particle Reynolds number of *** to the blocked-off method,the smooth IBM reflects the formation of additional jets and recirculation zones better right above the bed,though increasing the fluid mesh resolution improves the accuracy of the blocked-off ***,a more diffusive behaviour is found for the blocked-off simulations due to the stairstep representation,which is avoided by using interpolation stencils as in the smooth *** higher mesh refinement in the blocked-off IBM this effect can be reduced,but this also increases the computational effort.

关键词： Immersed boundary method Packed bed reactor Uniform particle packing

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Optimizing magneto-ionic performance in structure/composition-engineered ternary nitrides

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Journal of Materiomics 2024年第4期10卷 870-879页

作者： Zheng Ma P.Monalisha Zhengwei Tan Eva Pellicer Maciej O.Liedke Maik Butterling Ahmed G.Attallah Eric Hirschmann Andreas Wagner Fatima Ibrahim Mairbek Chshiev enric Menndez Jordi sort Departament de Física Universitat Autònoma de BarcelonaCerdanyola del Vallès08193Spain Institute of Radiation Physics Helmholtz-Zentrum Dresden–RossendorfDresden01328Germany University of Grenoble Alpes CEACNRSSPINTECGrenoble38000France Institut Universitaire de France Paris75231France InstitucióCatalana de Recerca i Estudis Avançats(ICREA) Pg.Lluís Companys 23Barcelona08010Spain

Magneto-ionics,an emerging approach to manipulate magnetism that relies on voltage-driven ion motion,holds the promise to boost energy efficiency in information technologies such as spintronic devices or future non-von Neumann computing *** this purpose,stability,reversibility,endurance,and ion motion rates need to be synergistically *** various ions,nitrogen has demonstrated superior magneto-ionic performance compared to classical species such as oxygen or ***,we show that ternary Co1−xFexN compound exhibits an unprecedented nitrogen magneto-ionic *** substitution of Co by Fe in binary CoN is shown to be favorable in terms of generated magnetization,cyclability and ion motion ***,the Co_(0.3)5Fe_(0.6)5N films exhibit an induced saturation magnetization of 1,500 emu/cm^(3),a magneto-ionic rate of 35.5 emu/(cm^(3)·s)and endurance exceeding 103 *** values significantly surpass those of other existing nitride and oxide *** improvement can be attributed to the larger saturation magnetization of Co_(0.35)Fe_(0.65) compared to individual Co and Fe,the nature and size of structural defects in as-grown films of different composition,and the dissimilar formation energies of Fe and Co with N in the various developed crystallographic structures.

关键词： Voltage control of magnetism Magneto-ionics Electrolyte gating Ternary nitrides Ion transport

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Debaryomyces hansenii supplementation in low fish meal diets promotes growth,modulates microbiota and enhances intestinal condition in juvenile marine fish

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Journal of Animal science and Biotechnology 2024年第1期15卷 253-276页

作者： Ignasi sanahuja Alberto Ruiz Joana P.Firmino Felipe E.Reyes-López Juan B.Ortiz-Delgado Eva Vallejos-Vidal Lluis Tort Dariel Tovar-Ramírez Isabel M.Cerezo Miguel A.Moriñigo Carmen sarasquete enric Gisbert Aquaculture Program Institute of Agrifood Research and Technology(IRTA)La Rapita 43540Spain Centro de Biotecnologia Acuicola Universidad de Santiago de ChileSantiagoChile Instituto de Ciencias Marinas de Andalucia(ICMAN-CsIC) Avda.Republica Saharaui no 2Campus Universitario Rio San PedroPuerto RealCadiz 11510Spain Nucleo de Investigaciones Aplicadas en Ciencias Veterinarias y Agronomicas Facultad de Medicina Veterinaria y AgronomiaUniversidad de Las AmericasSantiagoChile Department of Cell Biology Physiologyand ImmunologyUniversitat Autonoma de BarcelonaBarcelonaSpain Centro de Investigaciones Biologicas del Noroeste sC CIBNORLa PazMexico Department of Microbiology Instituto de Biotecnologia Y Desarrollo Azul(IBYDA)Faculty of SciencesUniversity of Malaga29010 MalagaSpain sCBI Bioinformatic UnitUniversity of Malaga29590 MalagaSpain

Background The development of a sustainable business model with social acceptance,makes necessary to develop new strategies to guarantee the growth,health,and well-being of farmed *** hansenii is a yeast species that can be used as a probiotic in aquaculture due to its capacity to i)promote cell proliferation and differen-tiation,ii)have immunostimulatory effects,iii)modulate gut microbiota,and/or iv)enhance the digestive *** provide inside into the effects of *** on juveniles of gilthead seabream(sparus aurata)condition,we inte-grated the evaluation of the main key performance indicators coupled with the integrative analysis of the intestine condition,through histological and microbiota state,and its transcriptomic *** After 70 days of a nutritional trial in which a diet with low levels of fishmeal(7%)was supplemented with 1.1%of ***(17.2×10^(5) CFU),an increase of ca.12%in somatic growth was observed together with an improve-ment in feed conversion in fish fed a yeast-supplemented *** terms of intestinal condition,this probiotic modu-lated gut microbiota without affecting the intestine cell organization,whereas an increase in the staining intensity of mucins rich in carboxylated and weakly sulphated glycoconjugates coupled with changes in the affinity for certain lectins were noted in goblet *** in microbiota were characterized by the reduction in abundance of several groups of Proteobacteria,especially those characterized as opportunistic *** microarrays-based transcrip-tomic analysis found 232 differential expressed genes in the anterior-mid intestine of ***,that were mostly related to metabolic,antioxidant,immune,and symbiotic *** Dietary administration of *** enhanced somatic growth and improved feed efficiency param-eters,results that were coupled to an improvement of intestinal condition as histochemical and transcriptomic tools *** probiotic ye

关键词： Debaryomyces hansenii Intestine condition Low fish meal diet Microbiota Transcriptomics Yeast probiotic

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Predictive clinical features of cardioembolic infarction in patients aged 85 years and older

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Journal of Geriatric Cardiology 2019年第11期16卷 793-799页

作者： Ana Maria Carbajo-García Jonatan Cortés AdriàArboix Joan Massons Laura Díez enric.vergés Jordi Arboix-Alió Luís García-Eroles Cerebrovascular Division Department of NeurologyHospital Universitari del Sagrat CorUniversitat de BarcelonaBarcelonaCataloniaSpain Department of sports sciences Ramon Llull UniversityFPCEE BlanquernaBarcelonaCataloniaSpain Director d’Organitzaciói sistemes d’Informació Gerència Territorial Metropolitana NordInstitut Catalàde la SalutBarcelonaCataloniaSpain

Objective To assess predictive clinical factors of cardioembolic infarction in very old patients(85 years of age and older).Methods Prospective hospital-based stroke registry("The sagrat Cor Hospital of Barcelona stroke Registry")is an acute-care teaching hospital in Barcelona,Catalonia,*** 956 first-ever cardioembolic stroke patients included in the stroke registry over a 24-year period,639 were younger than 85 years of age and 317 were 85 years or older(mean age:88.9 years).Demographics,clinical characteristics,risk factors and early outcome were *** of cardioembolic infarction in the oldest age group were assessed by multivariate *** In a logistic regression model based on demographics,risk factors,clinical features and complications,female gender(odds ratio[OR]=1.74,95%confidence interval[CI]:1.27–2.39),heart failure(OR=2.27,95%CI:1.46–3.56),altered consciousness(OR=1.76,95%CI:1.28–2.42),and infectious complications(OR=2.01,95%CI:1.39–2.91)were predictors of cardioembolic stroke in the oldest age *** contrast,heavy smoking,heart valve disease,hypertension,headache,early seizures,sensory deficit,and involvement of the posterior cerebral artery were independently associated with cardioembolic stroke in the younger *** Identification of a differential clinical profile of cardioembolic stroke between patients aged 85 years or more and those younger than 85 years helps clinicians to the optimal management of ischemic infarction in the oldest segment of the population.

关键词： Cardioembolic stroke Clinical features Ischemic infarction stroke Very old patients

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