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Biomimetic natural biomaterials for tissue engineering and regenerative medicine:new biosynthesis methods,recent advances,and emerging applications

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Military Medical Research 2024年第1期11卷 50-79页

作者： Shuai Liu Jiang-Ming Yu Yan-Chang Gan Xiao-Zhong Qiu Zhe-Chen Gao Huan Wang Shi-Xuan Chen Yuan Xiong Guo-Hui Liu Si-en Lin Alec McCarthy Johnson V.John Dai-Xu Wei Hong-Hao hou Guangdong Provincial Key Laboratory of Construction and Detection in Tissue engineering the Fifth Affiliated HospitalSchool of Basic Medical ScienceSouthern Medical UniversityGuangzhou 510900China Department of Orthopedics Tongren HospitalShanghai Jiao Tong UniversityShanghai 200336China The Eighth Affiliated Hospital Sun Yat-Sen UniversityShenzhen 518033GuangdongChina engineering Research Center of Clinical Functional Materials and Diagnosis&Treatment Devices of Zhejiang Province Wenzhou InstituteUniversity of Chinese Academy of SciencesWenzhou 325011ZhejiangChina Department of Orthopedics Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430022China Department of Orthopaedics and Traumatology Faculty of Medicinethe Chinese University of Hong KongHong Kong SAR 999077China Department of Functional Materials Terasaki Institute for Biomedical InnovationLos AngelesCA 90064USA Mary&Dick Holland Regenerative Medicine Program College of MedicineUniversity of Nebraska Medical CenterOmahaNE 68130USA Zigong Affiliated Hospital of Southwest Medical University Zigong Psychiatric Research CenterZigong Institute of Brain ScienceZigong 643002SichuanChina Key Laboratory of Resource Biology and Biotechnology in Western China Ministry of EducationSchool of MedicineDepartment of Life Sciences and MedicineNorthwest UniversityXi’an 710127China

Biomimetic materials have emerged as attractive and competitive alternatives for tissue engineering(TE)and regenerative *** contrast to conventional biomaterials or synthetic materials,biomimetic scaffolds based on natural biomaterial can offer cells a broad spectrum of biochemical and biophysical cues that mimic the in vivo extracellular matrix(ECM).Additionally,such materials have mechanical adaptability,micro-structure interconnectivity,and inherent bioactivity,making them ideal for the design of living implants for specific applications in TE and regenerative *** paper provides an overview for recent progress of biomimetic natural biomaterials(BNBMs),including advances in their preparation,functionality,potential applications and future *** highlight recent advances in the fabrication of BNBMs and outline general strategies for functionalizing and tailoring the BNBMs with various biological and physicochemical characteristics of native ***,we offer an overview of recent key advances in the functionalization and applications of versatile BNBMs for TE ***,we conclude by offering our perspective on open challenges and future developments in this rapidly-evolving field.

关键词： Biomimic Scaffold Biosynthesis Natural biomaterial Tissue engineering

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MXene@c-MWCNT Adhesive Silica Nanofiber Membranes enhancing Electromagnetic Interference Shielding and Thermal Insulation Performance in Extreme environments

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Nano-Micro Letters 2024年第10期16卷 82-98页

作者： Ziyuan han Yutao Niu Xuetao Shi Duo Pan Hu Liu Hua Qiu Weihua Chen Ben Bin Xu Zeinhom MEl-Bahy Hua hou Eman Ramadan Elsharkawy Mohammed AAmin Chuntai Liu Zhanhu Guo Key Laboratory of Materials Processing and Mold(Zhengzhou University) Ministry of EducationNational Engineering Research Center for Advanced Polymer Processing TechnologyZhengzhou UniversityZhengzhou 450002People's Republic of China School of Nano-Tech and Nano-Bionics University of Science and Technology of ChinaHefei 230026People's Republic of China Key Laboratory of Multifunctional Nanomaterials and Smart Systems Advanced Materials DivisionSuzhou Institute of Nano-Tech and Nano-BionicsChinese Academy of SciencesSuzhou 215123People's Republic of China Shaanxi Key Laboratory of Macromolecular Science and Technology School of Chemistry and Chemical EngineeringNorthwestern Polytechnical UniversityXi'an 710072People's Republic of China College of Chemistry&Green Catalysis Center Zhengzhou UniversityZhengzhou 450001People's Republic of China Mechanical and Construction engineering Faculty of Engineering and EnvironmentNorthumbria UniversityNewcastle Upon Tyne NE18UK Department of Chemistry Faculty of ScienceAl-Azhar UniversityNasr City 11884CairoEgypt Department of Chemistry Faculty of ScienceNorthern Border UniversityArarSaudi Arabia Department of Chemistry College of ScienceTaif UniversityP.O.Box 1109921944 TaifSaudi Arabia

A lightweight flexible thermally stable composite is fabricated by com-bining silica nanofiber membranes(SNM)with MXene@c-MWCNT hybrid *** flexible SNM with outstanding thermal insulation are prepared from tetraethyl orthosilicate hydrolysis and condensation by electrospinning and high-temperature calcination;the MXene@c-MWCNT_(x:y)films are prepared by vacuum filtration *** particular,the SNM and MXene@c-MWCNT_(6:4)as one unit layer(SMC_(1))are bonded together with 5 wt%polyvinyl alcohol(PVA)solution,which exhibits low thermal conductivity(0.066 W m^(-1)K^(-1))and good electromagnetic interference(EMI)shielding performance(average EMI SE_(T),37.8 dB).With the increase in func-tional unit layer,the overall thermal insulation performance of the whole composite film(SMC_(x))remains stable,and EMI shielding performance is greatly improved,especially for SMC_(3)with three unit layers,the average EMI SET is as high as 55.4 *** addition,the organic combination of rigid SNM and tough MXene@c-MWCNT_(6:4)makes SMC_(x)exhibit good mechanical tensile ***,SMC_(x)exhibit stable EMI shielding and excellent thermal insulation even in extreme heat and cold ***,this work provides a novel design idea and important reference value for EMI shielding and thermal insulation components used in extreme environmental protection equipment in the future.

关键词： SiO_(2)nanofiber membranes MXene@c-MWCNT Composite film Thermal insulation Electromagnetic interference shielding

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Corrosion resistance of in-situ Mg-Al hydrotalcite conversion film on AZ31 magnesium alloy by one-step formation

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Transactions of Nonferrous Metals Society of China 2015年第6期25卷 1917-1925页

作者：曾荣昌刘振国张芬李硕琦赫庆坤崔洪芝韩恩厚山东科技大学材料科学与工程学院青岛266590 山东科技大学省部共建矿山灾害预防控制国家重点实验室青岛266590 中国科学院金属研究所沈阳110016

In situ growth of nano-sized layered double hydroxides （LDH） conversion film on AZ31 alloy was synthesized by a urea hydrolysis method. The formation mechanism of the film was proposed. Firstly, the dissolved Mg2＋ ions deposited into a precursor film consisted of MgCO3 and Mgs（CO3）4（OH）2·4H2O; secondly, the precursor translated into the crystalline Mg（OH）2 in alkaline conditions; finally, the Mg2＋ ions in Mg（OH）z were replaced by A13＋ ions, Mg（OH）2 translated into the more stable LDH structure, simultaneously, the OH- ions in the interlayer were exchanged by CO32-, thus led to the formation of the LDH （Mg6Alz（OHh6CO3·4H2O） film. The results indicated that the LDH film characterized by interlocking plate-like nanostructures and ion-exchange ability significantly improved the corrosion resistance of the AZ31 Mg alloy.

关键词： magnesium alloy hydrotalcite conversion film corrosion resistance ion-exchange

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无定河流域不同水土流失区底栖动物生物完整性指数构建与健康评价

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湖泊科学 2024年第1期36卷 274-285,I0026-I0037页

作者：侯湘然潘保柱侯易明胡恩贺瑶冯治远西安理工大学西北旱区生态水利国家重点实验室西安710048 陕西省环境科学研究院西安710061

无定河流域位于黄土高原与毛乌素沙地过渡带,水土流失严重,生态环境具有脆弱性和波动性。于2021年春季(4月)和秋季(10月)对无定河流域上、中、下游及其6条支流和流域内的3个淤地坝开展水生态系统调查,旨在厘清无定河流域底栖动物群落特... 详细信息

无定河流域位于黄土高原与毛乌素沙地过渡带,水土流失严重,生态环境具有脆弱性和波动性。于2021年春季(4月)和秋季(10月)对无定河流域上、中、下游及其6条支流和流域内的3个淤地坝开展水生态系统调查,旨在厘清无定河流域底栖动物群落特征,构建底栖动物生物完整性指数并开展健康评价。无定河流域春季共采集到底栖动物105种,平均密度为181 ind./m^(2),平均生物量为0.760 g/m^(2),秋季共采集到底栖动物67种,平均密度为94 ind./m^(2),平均生物量为0.454 g/m^(2)。通过对两季度研究区域内底栖动物27个生物参数开展分布范围检验、判别能力分析和相关性分析,构建无定河流域底栖动物生物完整性指数,对全流域40个样点(6个参照点和34个受损点)进行B-IBI健康评价。评价结果表明,总体上无定河流域底栖动物生物完整性较好,40个样点中春季有19个处于健康或亚健康状态,秋季有23个处于健康或亚健康状态,其中无定河上、中游干支流大都以健康和亚健康为主,无定河下游干支流以及3个淤地坝水体健康状况较差。在不同水土流失类型区域,底栖动物群落特征和生物完整性评价具有显著性差异。本研究结果可为无定河流域河流健康评估提供科学依据。

关键词：无定河流域水土流失底栖动物完整性指数大型底栖无脊椎动物健康评价

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A fluorescence-enhanced inverse opal sensing film for multi-sources detection of formaldehyde

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Food Science and Human Wellness 2024年

作者： Xiaokang Lu Bo han Deyilei Wei Mingzhu Chu Haojie Ma Ran Li Xueyan hou Yuqi Zhang JiJiang Wang Key Laboratory of New energy & New Functional Materials， Shaanxi Key Laboratory of Chemical Reaction engineering， College of Chemistry and Chemical engineering， Yan’an University

The SiO2inverse opal photonic crystals (PC) with a three-dimensional macroporous structure were fabricated by the sacrificial template method， followed by infiltration of a pyrene derivative， 1-(pyren-8-yl)but-3-en-1-amine (PEA)， to achieve a formaldehyde-sensitive and fluorescence-enhanced sensing film. Utilizing the specific Aza-Cope rearrangement reaction of allylamine of PEA and formaldehyde (FA) to generate a strong fluorescent product emitted at ～480 nm， we chose a PC whose blue band edge of stopband overlapped with the fluorescence emission wavelength. In virtue of the fluorescence enhancement property derived from slow photon effect of PC， FA was detected highly selectively and sensitively. The limit of detection (LoD) was calculated to be 1.38 nmol/L. Furthermore， the fast detection of FA (within 1 min) is realized due to the interconnected three-dimensional macroporous structure of the inverse opal PC and its high specific surface area. The prepared sensing film can be used for the detection of FA in air， aquatic products and living cells. The very close FA content in indoor air to the result from FA detector， the recovery rate of 101.5% for detecting FA in aquatic products and fast fluorescence imaging in 2 min for living cells demonstrate the reliability and accuracy of our method in practical applications.

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Influence of solution temperature on corrosion resistance of Zn-Ca phosphate conversion coating on biomedical Mg-Li-Ca alloys

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Transactions of Nonferrous Metals Society of China 2013年第11期23卷 3293-3299页

作者：曾荣昌孙芯芯宋影伟张芬李硕琦崔洪芝韩恩厚山东科技大学材料科学与工程学院青岛266590 中国科学院金属研究所金属腐蚀与防护国家重点实验沈阳110016

The influence of phosphating bath at different temperatures on the formation and corrosion property of calcium-modified zinc phosphate conversion coating （Zn-Ca-P coating） on Mg-Li-Ca alloy was investigated. The morphologies, elemental distribution and chemical structures of the coatings were examined via SEM, EPMA, EDS, XRD and FT-IR. The corrosion resistance was assessed by hydrogen evolution, potentiodynamic polarization and EIS. The results show that the coating is composed of single element Zn and ZnO at below 45 ℃;whereas the coatings are predominantly characterized by Zn3（PO4）2·4H2O and small amount of element zinc and ZnO at above 50 ℃. Mg-Li-Ca alloy with Zn-Ca-P coatings prepared at 55 ℃ has the highest corrosion resistance. However, the hydrogen evolution rates of the coatings obtained at 40-50 ℃ is accelerated due to the galvanic corrosion between the imperfection of the single element Zn coating and the Mg substrate.

关键词： magnesium alloy lithium calcium phosphate conversion coating corrosion biomaterial

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Facet-dependent CuO/{010}BiVO_(4)S-scheme photocatalyst enhanced peroxymonosulfate activation for efficient norfloxacin removal

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Journal of Materials Science & Technology 2024年第7期174卷 30-43页

作者： Tongyu han Haifeng Shi Yigang Chen School of Science Jiangnan UniversityWuxi 214122China Department of General Surgery Jiangnan University Medical CenterWuxi 214002China

Rapid recombination of charge carriers and sluggish Cu^(2+)/Cu^(+)conversion rate in Cu-based photocatalysts hinder the improvement of the peroxymonosulfate(PMS)activation ***,a novel S-scheme system was successfully built through hydrothermal and in-situ calcination methods to activate PMS for norfloxacin(NOR)degradation,which combined CuO with BiVO_(4)(BVO)containing surface *** UV-vis spectra manifested that BVO displayed excellent visible light absorption performance after compounding with CuO,and the light absorption threshold of CuO/BVO was about 600 *** to the existence of surface heterojunction in BVO,the photoinduced electrons,and holes would trans-fer to{010}and{110}facets,*** construction of S-scheme heterojunction further facilitated the accumulation of electrons on CuO,thus realizing the spatial separation of charge *** addi-tion,the electrons gathered on the CuO expedited the Cu^(2+)/Cu^(+)cycle,thereby improving the activation efficiency of *** this basis,the NOR removal capacity of 5CuO/BVO composites was obviously en-hanced,which was 3.65 and 2.45 times that of CuO and ***,the influence of ambient pH and PMS dosage on the photocatalytic performance of CuO/BVO was *** the analysis of NOR degradation pathways and degradation products,it was found that the toxicity threat of NOR to the environment was reduced during the degradation *** to the XPS results,forming the S-scheme heterojunction accelerated the Cu^(2+)/Cu^(+)redox cycle during the PMS activating ***,photoluminescence(PL)and time-resolved photoluminescence(TRPL)analysis demonstrated that the CuO/BVO composites exhibited eminent ability for charge *** possible mechanism of charge transfer was assumed by exploring reactive species and the energy band structure of *** sum up,this research provides a new perspective on boosting PMS activation to purify antibiotics

关键词： Norfloxacin degradation Peroxymonosulfate S-scheme heterojunction Surface heterojunction Charge separation

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CRISPR/Cas9-mediated knockout of E4 gene promotes maturation in soybean

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Oil Crop Science 2024年第3期9卷 170-176页

作者： Shuiqing Wu Li Chen Mengwei Guo Yupeng Cai Yang Gao Shan Yuan Shi Sun Yuxian Zhang Wensheng hou Tianfu han College of Agriculture Heilongjiang Bayi Agricultural UniversityDaqing163316China Ministry of Agriculture Key Laboratory of Soybean Biology(Beijing) Institute of Crop SciencesChinese Academy of Agricultural SciencesBeijing100081China College of Agriculture Northeast Agricultural UniversityHarbin150030China State Key Laboratory of Crop Germplasm Innovation and Molecular Breeding Syngenta Biotechnology(China)Co.LtdBeijing100081China

Soybean is a broadly popular and extensively cultivated crop,however,many high-yield and high-quality varieties require specific growth conditions,restricting their widespread *** appropriate light conditions and photoperiod must be attained for these varieties to thrive in new *** this study,we employed CRISPR/Cas9 to design two sgRNAs aimed at knocking out the maturity-related gene E4 in a major American soybean variety called''Jack'',which belongs to maturity group MGII.E4 gene is primarily involved in the photoperiodic flowering and maturity in soybean,making it an ideal candidate for genetic *** successfully obtained 1 homozygous E4-SG1 mutant type with 1-bp insertion,and 4 homozygous E4-SG2 mutants type with 2-bp deletion,7-bp deletion,61-bp deletion,and 1-bp insertion,*** homozygous e4 mutant plants contained early termination codons devoid of transgenic ***,no potential offtarget sites of the E4 gene were detected.A comparative analysis revealed that,unlike the wild-type,the maturity time of homozygous e4 mutants was early under both short-day and long-day *** mutants offer novel germplasm resources that may be used to modify the photoperiod sensitivity and maturity of soybean,enhancing its adaptability to high-latitude regions.

关键词： Soybean E4 CRISPR/Cas9 Maturity

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Evidence for distortion-induced local electric polarization in α-RuCl_(3)

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Science China(Physics,Mechanics & Astronomy) 2024年第9期67卷 165-173页

作者： Xinrun Mi De hou Xiao Wang Sabreen Hammouda Caixing Liu Zijian Xiong han Li Yan Liu Guiwen Wang Aifeng Wang Yisheng Chai Yang Qi Wei Li Xiaoyuan Zhou Yixi Su DIKhomskii Mingquan He Zhigao Sheng Young Sun College of Physics Center of Quantum Materials and DevicesChongqing UniversityChongqing 401331China Anhui Key Laboratory of Condensed Matter Physics at Extreme Conditions High Magnetic Field LaboratoryHefei Institutes of Physical ScienceAnhuiChinese Academy of SciencesHefei 230031 China University of Science and Technology of China Hefei 230026China Jülich Centre for Neutron Scienceat Heinz Maier-Leibnitz Zentrum(JCNS-MLZ) Forschungszentrum Jülich GmbHGarching D-85747Germany School of Physics Beihang UniversityBeijing 100191China Analytical and Testing Center Chongqing UniversityChongqing 401331China State Key Laboratory of Surface Physics Department of PhysicsFudan UniversityShanghai 200433China CAS Key Laboratory of Theoretical Physics Institute of Theoretical PhysicsChinese Academy of SciencesBeijing 100190China Ⅱ.Physikalisches Institut Universität zu KölnKöln D-50937Germany

The spin-orbit assisted Mott insulator α-RuCl_(3) is a prime candidate for material realization of the Kitaev quantum spin *** little attention has been paid to charge degrees of freedom,charge effects,such as electric polarization,may arise in this ***,we report distortion-induced local electric polarization in α-RuCl_(3) as evidenced by single-crystal X-ray diffraction,second harmonic generation(SHG)and dielectric *** SHG signal appears at room temperature and develops substantially in the Kitaev paramagnetic state when short-range spin correlations come into *** sizable pyroelectric currents in the Kitaev paramagnetic state,the absence of hysteresis in the polarization-electric field(P-E)points to the shortrange nature of electric *** localized electric polarization is likely the result of distortion-induced charge dimerization,achieved through virtual hopping-induced charge *** addition,the electric polarization is boosted by short-range spin correlations via spin-phonon coupling in the Kitaev paramagnetic *** results emphasize the importance of charge degrees of freedom in α-RuCl_(3),which establish a novel platform to investigate charge effects in Kitaev materials.

关键词： Mott insulator Kitaev quantum spin liquid electric polarization

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Exploration of pathogenic microorganism within the small intestine of necrotizing enterocolitis

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World Journal of Pediatrics 2024年第2期20卷 165-172页

作者： Yan Wang Kun Jiang Qiao Xia Xia Kang Shan Wang Ji-Hong Yu Wen-Feng Ni Xiao-Qin Qi Ying-Na Zhang Jin-Bao han Gang Liu Lei hou Zhi-Chun Feng Liu-Ming Huang Clinical Biobank Center Medical Innovation Research Division of ChinesePLA General HospitalBeijingChina Institute of Basic Medical Sciences Chinese Academy of Medical Sciences and Peking Union Medical CollegeBeijingChina Senior Department of Pediatrics The Seventh Medical Center of PLA General HospitalBeijing 100700China Department of Neurology The First Medical CenterChinese PLA General HospitalBeijing 100853China Department of Emergency Center National Center for Children's HealthBeijing Children's HospitalCapital Medical UniversityBeijing 100045China

Background Necrotizing enterocolitis(NEC)is the most common severe gastrointestinal emergency in *** designed this study to identify the pathogenic microorganisms of NEC in the microbiota of the small intestine of *** Using the 16S ribosomal DNA(rDNA)sequencing method,we compared and analyzed the structure and diversity of microbiotas in the intestinal feces of different groups of neonates:patients undergoing jejunostomy to treat NEC(NP group),neonates undergoing jejunostomy to treat other conditions(NN group),and neonates with NEC undergoing conservative treatment(NC group).We took intestinal feces and saliva samples from patients at different time *** The beta diversities of the NP,NN,and NC groups were all *** comparing the beta diversities between different time points in the NP group,we found similar beta diversities at time points El to E3 but significant differences between the E2-E3 and E4 time points:the abundances of Klebsiella and enterococcus(Proteobacteria)were higher at the E1-E3 time points;the abundance of Escherichia-Shigella(Proteobacteria)increased at the E2 time point,and the abundance of Klebsiella decreased significantly,whereas that of Streptococcus increased significantly at the E4 time *** Our results suggest that the pathological changes of intestinal necrosis in the small intestine of infants with NEC are not directly caused by excessive proliferation of pathogenic bacteria in the small *** sources of microbiota in the small intestine of neonates,especially in premature infants,may be affected by multiple factors.

关键词： Microbiota Necrotizing enterocolitis-Small intestine 16S rDNA

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