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New steroid dimer derived from cholic acid as receptor for lanthanum(Ⅲ) and calcium(Ⅱ) nitrates

Journal of Rare Earths 2010年第S1期28卷 56-60页

作者： Zdzisaw Paryzek Monika Piasecka Roman Joachimiak emilia luks Wanda Radecka-Paryzek Faculty of Chemistry Adam Mickiewicz University

New steroid dimer,ethylene bridged bis-carbamate(3) was synthesized from cholic acid in few steps. Complexes of 3 with lanthanum(Ⅲ) and calcium(Ⅱ) nitrates were prepared in reaction of 3 and appropriate metal salts. They were characterized by spectral data(Infrared(IR) ,ultraviolet-visible(UV/Vis) ,nuclear magnetic resonance(NMR) spectroscopy and fast-atom bombardment(FAB) ,electrospray(ESI) and matrix-assisted laser desorption/ionization mass spectrometry(MALDI-MS)) and elemental analysis. The similarity in complexing behavior of steroidal dimer toward calcium and lanthanum ions was observed.

关键词： cholic acid steroid dimer carbamate lanthanum and calcium nitrate complexes rare earths

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Molecular Diversity of <i>Staphylococcus aureus</i>from the Nares of Hospital Personnel, HIV-Positive and Diabetes Mellitus Patients in Yaounde Cameroon

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Advances in Microbiology 2021年第12期11卷 740-756页

作者： Agnes Eyoh Marthie Ehlers emilia Lyonga Mbamyah John Antiabong Charles Fokunang Marleen Kock Marie Claire Okomo Assoumou Michel Toukam Hortense Gonsu Kamga George Ikomey Martha Mesembe Mandi Henshaw Christiana Haddison Sinata Koulla-Shiro Center for the Study and Control of Communicable Diseases Faculty of Medicine and Biomedical Sciences The University of Yaounde I Yaoundé Cameroon Faculty of Medicine and Biomedical Sciences The University of Yaounde I Yaoundé Cameroon Department of Medical Microbiology University of Pretoria/National Health Laboratory Service (NHLS) Pretoria South Africa Ministry of Public Health Yaoundé Cameroon

Nasal carriage of Staphylococcus aureus has been identified as a risk factor for the development of staphylococcal infections caused by endogenous colonizing strains. Information on the genotypic diversity of Staphylococcus aureus is relevant for managing epidemiological and clinical challenges resulting from the evolutionary differences of this bacterium. The objective of this study was to determine and compare the molecular diversity of Staphylococcus aureus isolates from three high-risk populations in Yaounde, Cameroon. Molecular analysis confirmed that 95% of 100 tested isolates were S. aureus. The mecA and Panton Valentine-Leukocidin (PVL) genes (luks/F-PV) were detected in 37% (35/95) and 43% (41/95) of isolates respectively and 18% (17/95) of the isolates harboured both the mecA and luks/F-PV genes. A mixed distribution of both methicillin sensitive S. aureus (MSSA)/PVL and methicillin resistant S. aureus (MRSA)/PVL strains were detected within the study population. Community associated MRSA accounted for 94% (33/35) of the isolates, further classified into allotypes SCCmec type IV 54% (19/35) and SCCmec type V 40% (14/35), while two isolates were hospital associated SCCmec type II strains. A majority of the isolates harboured a single aggressive gene regulator allele agr type I. Pulsed Field Gel Electrophoresis (PFGE) generated 18 pulsotypes that grouped isolates irrespective of the study population. Multilocus Sequence Typing (MLST) of 12 selected isolates was assigned to six pandemic clonal complexes (CC): CC5 (ST5), CC8 [ST8, (n = 3)], CC15 (ST 15), CC25 (ST 25), CC72 [ST72 (n = 2)] and CC121 [ST 121 (n = 2)] and three atypical sequence types ST 508, ST 699 (CC45) and ST 1289 (CC 88). The study population represents an important reservoir for MRSA, MRSA-PVL and MSSA-PVL which could serve as focal point for further dissemination bringing about significant clin

关键词： Nasal Carriage Methicillin Resistant S. aureus Methicillin Sensitive S. aureus Panton-Valentine Leukocidin Multilocus Sequence Typing

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