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Science China(Life Sciences) 2019年第4期62卷 526-534页

作者： Vandeclécio Lira da Silva André Mauricio Ribeiro dos santos Wilfredo Blanco Sandro José de Souza Bioinformatics Multidisciplinary Environment (BioME) Universidade Federal do Rio Grande do Norte (UFRN) Programa de Pós-Gradua??o em Genética e Biologia Molecular (PPGBM) Universidade Federal do Pará (UFPA) Department of Computer Science Universidade Estadual do Rio Grande do Norte (UERN) Brain Institute Universidade Federal do Rio Grande do Norte (UFRN)

The gain of transcription factor binding sites(TFBS) is believed to represent one of the major causes of biological *** we used strategies based on comparative genomics to identify 21,822 TFBS specific to the human lineage(TFBS-HS),when compared to chimpanzee and gorilla genomes. More than 40%(9,206) of these TFBS-HS are in the vicinity of 1,283 genes.A comparison of the expression pattern of these genes and the corresponding orthologs in chimpanzee and gorilla identified genes differentially expressed in human tissues. These genes show a more divergent expression pattern in the human testis and brain, suggesting a role for positive selection in the fixation of TFBS gains. Genes associated with TFBS-HS were enriched in gene ontology categories related to transcriptional regulation, signaling, differentiation/development and nervous ***, genes associated with TFBS-HS present a higher expression breadth when compared to genes in general. This biased distribution is due to a preferential gain of TFBS in genes with higher expression breadth rather than a shift in the expression pattern after the gain of TFBS.

关键词： TFBS transcript factor human evolution expression breadth

Immunohistochemistry panel segregates molecular types of hepatocellular carcinoma in Brazilian autopsy cases

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World Journal of Gastroenterology 2016年第27期22卷 6246-6256页

作者： Aloísio Felipe-Silva Alda Wakamatsu Cinthya dos santos Cirqueira Venancio Avancini Ferreira Alves LIM 14-Patologia Hepatica Faculdade de Medicina da USP01246-903 Sao Paulo SPBrazil Department of Pathology Medical Research Laboratory LIM14University of Sao Paulo School Of Medicine01246-903 Sao Paulo SPBrazil

AIM: To assess the distribution of proteins coded by genes reported as relevant for the molecular classification of hepatocellular carcinoma (HCC).METHODS: In this retrospective cross-sectional study, the following clinicopathological data were analyzed in 80 autopsied HCC patients: sex, age, ethnicity, alcohol intake, infection with hepatitis B and/or C virus, infection with human immunodeficiency virus, prior treatment, basic and immediate causes of death, liver weight, presence of cirrhosis, number and size of nodules, gross pattern, histological grade and variants, architectural pattern, invasion of large veins, and presence and location of extrahepatic metastases. The protein products of genes known to be involved in molecular pathogenesis of HCC, including epidermal growth factor receptor (EGFR), MET, keratin 19 (K19), vimentin, beta-catenin, mechanistic target of rapamycin (mTOR), extracellular signaling-related kinase (ERK)1, ERK2, Ki67, cyclin D1, caspase 3 and p53, were detected by immunohistochemistry on tissue microarrays. The expression levels were scored and statistically assessed for correlation with HCC ***: Infection with hepatitis C virus was identified in 49% of the 80 autopsy patients, cirrhosis in 90%, advanced tumors in 95%, and extrahepatic metastases in 38%. Expression of K19, p53 and ERK1 correlated to high-grade lesions. Expression of ERK1, nuclear beta-catenin, cyclin D1 and ERK2 correlated to higher rates of cell proliferation as determined by Ki67. Expression of MET, EGFR (> 0) and caspase 3 correlated with lower histological grades. Expression of EGFR correlated to that of caspase 3, and overexpression of EGFR (≥ 200/300) was observed in low-grade tumors more frequently (grades 1 and 2: 67% vs grade 3: 27% and grade 4: 30%). Expression of ERK1 was associated with that of K19 and vimentin, whereas expression of ERK2 was associated with that of cyclin D1, MET and membrane beta-catenin. Expression of vimentin

关键词： Hepatocellular carcinoma Epidermal growth factor receptor Autopsy Immunohistochemistry Liver Classification

Direct Electrochemical Determination of Glyphosate Herbicide Using a Screen-Printed Carbon Electrode Modified with Carbon Black and Niobium Nanoparticles

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Journal of Analysis and Testing 2023年第4期7卷 425-434页

作者： Julia Oliveira Fernandes Cassiano Augusto Rolim Bernardino Juliana dos santos Fernandes Claudio Fernando Mahler Bernardo Ferreira Braz Luiz Henrique Carpenter dos santos Rodrigo JoséCorrêa Ricardo Erthal Santelli Braulio Soares Archanjo Emerson Schwingel Ribeiro Fernando Henrique Cincotto Departament of Analytical Chemistry Institute of ChemistryFederal University of Rio de JaneiroRio de Janeiro 21941-909Brazil Departament of Civil Engineering COPPEFederal University of Rio de JaneiroRio de Janeiro 21941-598Brazil National Institute of Science&Technology of Bioanalytics(INCTBio) CampinasSão Paulo 13083-862Brazil Departament of Inorganic Chemistry Institute of ChemistryFederal University of Rio de JaneiroRio de Janeiro 21941-909Brazil Unesp National Institute of Alternative Technologies for DetectionToxicological Assessment and Removal of Micropollutants and Radioactives(INCT-DATREM)AraraquaraSão Paulo 14800-060Brazil National Institute of Metrology Quality and TechnologyInmetroAv.Nossa Senhora das Graças50XerémDuque de Caxias 25250-020Brazil

Glyphosate is a herbicide that has been widely used worldwide and is used in agricultural areas to control weeds and unwanted *** sensors developed from different nanomaterials have high efficiency,excellent cost-benefit,and fast analysis time for detecting traces of environmental *** study aimed to produce an electrochemical sensor with disposable screen-printed electrodes based on carbon black modified with niobium nanoparticles to determine glyphosate in aqueous *** morphology,structure and electrochemical performance of the sensor were characterized by scanning electron microscopy,energy-dispersive X-ray spectroscopy,X-ray diffraction and cyclic *** pulse voltammetry in BR buffer solution at pH 5.0 allowed the generation of a method to quantify glyphosate concentration in a linear range of 5.90-172.30μmol/L(1.00-29.13μg/mL),with a limit of detection calculated at 3.07μmol/L(0.52μg/mL).The method efficiently quantified glyphosate in real water samples and showed no interference from K^(+),Na^(+),Ca^(2+),Mg^(2+)ions or thiamethoxam,imidacloprid and carbendazim pesticides.

关键词： Glyphosate Electrochemical sensor Carbon black Niobium nanoparticles

Influence of Sugarcane Bagasse Fiber Size on Biodegradable Composites of Thermoplastic Starch

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Journal of Renewable Materials 2018年第2期6卷 176-182页

作者： Bruno Henrique dos santos Karen de Souza do Prado Asaph Armando Jacinto Marcia Aparecida da Silva Spinace Federal University of ABC Center of Natural and Human SciencesAv.dos Estados5001Santa Terezinha09.210-170 Santo AndréSão PauloBrazil

Although thermoplastic starch(TPS)is biodegradable,its low mechanical resistance limits its wide *** bagasse(SB)fibers can be used as reinforcement in TPS matrix composites,but the influence of fiber size on the properties of the composite is still *** this study,TPS composites reinforced with SB short fibers of four sizes were processed and characterized in order to analyze the influence of fiber size on the mechanical properties of the TPS/SB *** was observed that the interaction between fiber and matrix was good and optimized when the fibers are sifted in sieves between 30 and 50 mesh,obtaining fibers with average length of 1569±640μm and average diameter of 646±166μ*** these composites,increases of more than 660%in the modulus and more than 100%in the maximum tension were verified when compared to the pure TPS.

关键词： Sugarcane bagasse thermoplastic starch composite fiber size

Cardiovascular risk burden and disability:findings from the International Mobility in Aging Study(IMIAS)

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Journal of Geriatric Cardiology 2024年第3期21卷 331-339页

作者： Juan-David Martinez-Aristizábal Carmen-Lucia Curcio Juliana Fernandes Afshin Vafael Cristiano dos santos Gomes Fernando Gomez Internal Medicine Critical Care DepartmentPablo Tobón Uribe HospitalMedellínColombia Research Group in Geriatrics and Gerontology Faculty of Health SciencesUniversidad de CaldasManizalesColombia Laboratory of Physical Therapy and Collective Health Physical Therapy DepartmentFederal University of PernambucoJornalista Aníbal Fernandes Avenue RecifeBrazil Department of Public Health Sciences Queen’s UniversityKingstonONCanada Department of Physiotherapy University of PernambucoBR 203KmBrazil

BACKGROUND The association of cardiovascular risk burden with disability is *** examined the association between trajectories of the Framingham general cardiovascular disease risk score(FGCRS)with the trajectories of limitations of physical function in older *** A total of 1219 participants with no disabilities from the International Mobility in Aging Study(IMIAS)study who had up to three repeated measures of FGCRS between 2012–2016 and without a history of stroke or coronary heart disease at baseline and follow-up were *** at baseline was assessed and categorized into *** function was evaluated with the Short Physical Performance Battery(SPPB).The data were analyzed using linear mixed-effects *** At baseline,FGCRS ranged between 3–94(mean score:24±15.8),participants were 32(2.6%),502(41.2%)and 685(56.2%)in lowest,middle,and highest tertiles,*** the trajectories of limitations of physical function,the lowest FGCRS had no differences,while the middle and highest had a decrease in physical performance between 2012–2014(P=0.0001).Age,being female,living in Andes Mountains,having middle and highest FGCRS,higher alcohol consumption,being obese,lack of exercise and cognitive impairment increase the probability of disability(P<0.05).Alternatively,living in more developed regions and having a higher educational level reduced the probability of disability during the follow-up time(P<0.05).CONCLUSIONS Higher cardiovascular risk burden is associated with decreased physical performance,especially in *** suggest SPPB may provide a measure of cardiovascular health in older adults.

关键词： finding Battery burden

Physical habitat condition as a key tool to maintain freshwater biodiversity in neotropical artificial ponds

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Water Biology and Security 2023年第3期2卷 30-35页

作者： Marden S.Linares Livia B.dos santos Marcos Callisto Jean C.santos Universidade Federal de Minas Gerais Instituto de Ciencias BiologicasDepartamento de GeneticaEcologia e EvoluçaoLaboratorio de Ecologia de BentosAv.Antonio Carlos 6627CEP 31270-901PampulhaBelo HorizonteMGBrazil Laboratorio de Macroecologia e Conservaçao da Biodiversidade Departamento de Botanica e EcologiaUniversidade Federal do Mato GrossoAv.Fernando Correa da Costa2367Boa EsperançaCuiabaMato GrossoCEP 78060-900Brazil Programa de Pos-Graduaçao em Ciencias Ambientais Instituto de Ciencias NaturaisHumanas e SociaisUniversidade Federal do Mato GrossoAvenida Alexandre Ferronato1200SinopMTCEP 78550-728Brazil Universidade Federal de Sergipe Departamento de EcologiaCampus Sao Cristovao-Cidade Universitaria Prof.Jose Aloísio de CamposCEP 49100-000Sao CristovaoSergipeBrazil

In areas highly affected by anthropogenic disturbances,artificial(human-made)freshwater ecosystems can provide habitat for maintaining and conserving regional freshwater *** assessed how the physical habitat of artificial ponds affected the structure of benthic macroinvertebrate *** do so,we tested two hypotheses.(1)Physical habitat disturbances are not detrimental to the diversity of nearby artificial ponds,and(2)Physical habitat disturbances do not cause significant shifts in taxonomic *** results rejected both null hypotheses,i.e.,macroinvertebrate diversity metrics correlated significantly and positively with Physical Habitat Integrity index scores,and only sites with high habitat condition scores were significantly associated with sensitive indicator *** results highlight the importance of maintaining physical habitat conditions for sustaining the ecological health of artificial ponds.

关键词： Bioindicators Ecosystem management Lentic ecosystems

Soil properties characterization for land-use planning and soil management in watersheds under family farming

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International Soil and Water Conservation Research 2022年第1期10卷 119-128页

作者： Jose Miguel Reichert Paulo Ivonir Gubiani Danilo Rheinheimer dos santos Dalvan Jose Reinert Celso Aita Sandro Jose Giacomini Soils Department Universidade Federal de Santa Maria(UFSM)Santa MariaRSBrazil

Environmental monitoring of small,rural watersheds was one of the components of the Natural Re-sources Management and Rural Poverty Alleviation Program(RS-Rural)in southern *** purpose of the monitoring was to assess the impact of promoting soil conservation and environment manage-ment practices adopted by farmers and funded by the *** four small monitored watersheds,in a total of 95 plots representing distinct land use and soil management,surface soil was collected to characterize ground-zero of the Program by determining several soil physical,chemical and microbio-logical *** component analysis(PCA)shows soil physical,chemical and biological properties were decisive in defining the agricultural soils in the rural watersheds with family *** sensitivity to chemical properties provides an opportunity to improve soil quality if soil management focuses on altering those *** management practiced by tobacco farmers leads to rapid,intense degradation of some natural soil properties,especially those related to the dynamics of soil organic matter,compared with more conservationist uses(forest,regrowth,and grassland).Thus,soil management must be reoriented to avoid the progress of degradation and recover soil physical and biological *** crops and by land-abandonment to allow natural vegetation are important management strategies for the degraded soils used for tobacco production,increasing soil organic matter,nutrients and microbial activity and thus allowing further crop *** conclusion,wa-tersheds with tobacco cropping have soils with lower quality than when under no-tillage grain pro-duction,requiring changes in land use and soil management.

关键词： Natural resources management Environmental monitoring Soil conservation Soil quality Alleviating poverty

Dealing with Multisource Information for Estuarine Flood Risk Appraisal in Two Western European Coastal Areas

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International Journal of Disaster Risk Science 2022年第2期13卷 199-213页

作者： Ana Rodrigues Rilo Alexandre Manuel de Oliveira Soares Tavares Paula Maria dos santos Freire José Luis Zêzere Estuarine and Coastal Zones Unit LNEC-National Civil Engineering Laboratory.1700-075 LisbonPortugal Centre of Geographical Studies Institute of Geography and Spatial PlanningUniversidade de Lisboa1700-066 LisbonPortugal Centre for Social Studies and Earth Sciences Department Coimbra University3030-790 CoimbraPortugal

Estuaries are usually affected by compound flooding triggers that cause diverse territorial *** fluvial flood risk assessment frameworks are well established in the literature,integrated management instruments that deal with estuarine flood risk remain incomplete and often *** research presents a methodology to extract relevant information from multiple sources post-event and a database building process that is applied to two contrasting estuaries(the Tagus River estuary in Portugal,and the Shannon River estuary in Ireland)in the Western European coastal ***,a total of 274 documents were analyzed and the information was stored in two *** correspondence analysis was applied to extract the most informative and relevant estuarine flood *** integrated estuarine flood risk assessment framework is presented and discussed based on the extracted *** framework is driven by two distinct dimensions(oceanic and hydrographic)and revealed the transversal position of triggers of estuarine floods,reflecting the compounding effects usually present in these *** results also highlight two levels of flood risk mostly based on damage typology.

关键词： Estuarine floods Estuarine flood indicators Multiple correspondence analysis Post-hazard multisource information Western Europe

Development of Junction Elements from Study of the Bionics

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Journal of Bionic Engineering 2007年第1期4卷 41-46页

作者： Wilson Kindlein Junior Luis Henrique Alves Cndido André Canal Marques Sandra Souza dos santos Maurício da Silva Viegas Laboratory of Design and Material Selection-LdSM-Materials Department (DEMAT) School of Engineering–EE Federal University of Rio Grande do Sul-Brazil-UFRGS Brazil

The applications of bionic methodology developed by the Laboratory of Design and Material Selection as basis in the creation of junction elements were demonstrated. These elements favor the application of Ecodesign in reference to the effectiveness of product dismount aiming the reduction of ambient impact in all its phases of use. The creation, the development and the confection of new junction elements were described, and case studies of new products developed specifically with this purpose were presented.

关键词： bionics Ecodesign junction elements

Improving weld formability by a novel dual-rotation bobbin tool friction stir welding

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Journal of Materials Science & Technology 2018年第1期34卷 135-139页

作者： F.F.Wang W.Y.Li J.Shen Q.Wen J.F.dos santos State Key Laboratory of Solidification Processing Shaanxi Key Laboratory of Friction Welding Technologies Northwestern Polytechnical University Helmholtz-Zentrum Geesthacht Institute of Materials Research Materials Mechanics China Academy of Launch Vehicle Technology Beijing Institute of Astronautical Systems Engineering

A novel dual-rotation bobbin tool friction stir welding （DBT-FSW） was developed, in which the upper shoulder （US） and lower shoulder （LS） have different rotational speeds. This process was tried to weld 3.2 mm thick alunlinum-lithium alloy sheets. The metallographic analysis and torque measurement were carried out to characterize the weld formabiliW. Experimental results show that compared to conven- tional bobbin tool friction stir welding, the DBT-FSW has an excellent process stability, and can produce the defect-free joints in a wider range of welding parameters. These can be attributed to the significant improvement of material flow caused by the formation of a staggered layer structure and the unbalanced force between the US and LS during the DBT-FSW process.

关键词： Bobbin tool friction stir welding Dual rotation Material flow Microstructure Microhardness