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The house of mirth

Fertility,genome stability,and homozygosity in a diverse set of resynthesized rapeseed lines

作者： Edith Wharton edited by elizabeth Ammons.

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The Crop Journal 2023年第2期11卷 468-477页

作者： elizabeth Ihien Katche Antje Schierholt Heiko C.Becker Jacqueline Batley Annaliese S.Mason Plant Breeding Department The University of Bonn53115 BonnGermany Department of Plant Breeding Justus Liebig University35392 GiessenGermany Department of Crop Sciences Division of Plant Breeding MethodologyGeorg-August University Göttingen37073 GöttingenGermany School of Biological Sciences The University of Western AustraliaPerthWA 6009Australia

Rapeseed(Brassica napus,AACC)was formed by hybridization between progenitor species Brassica rapa(AA)and Brassica oleracea(CC).As a result of a limited number of hybridization events between specific progenitor genotypes and strong breeding selection for oil quality traits,rapeseed has limited genetic *** production of resynthesized *** lines via interspecific hybridization of the diploid progenitor species *** and *** is one possible way to increase genetic variation in ***,most resynthesized lines produced so far have been reported to be meiotically unstable and infertile,in contrast to established *** *** hinders both maintenance and use of this germplasm in breeding *** characterized a large set of 140 resynthesized lines produced by crosses between *** and ***,as well as between *** and wild C genome species(***,***,***,***,*** and ***)for purity(homozygosity),fertility,and genome ***-pollinated seed set,seeds per ten pods as well as percentage pollen viability were used to estimate *** genotyping was performed using the Illumina Infinium Brassica 60K array for 116 genotypes,with at least three individuals per *** of the material which had been advanced through multiple generations was no longer pure,with heterozygosity detected corresponding to unknown parental contributions via *** and genome stability were both *** lines had high numbers of copy number variants(CNVs),indicative of meiotic instability,and high numbers of CNVs were significantly associated with reduced *** putatively stable resynthesized *** lines were *** investigation of these lines may reveal the mechanisms underlying this *** results suggest that selection of stable resynthesized lines for breeding purposes is possible.

关键词： Fertility Genome stability Copy number variants SNP genotyping Resynthesized lines

Pseudomonas exotoxin antisense RNA selectively kills hepatitis B virus infected cells

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World Journal of Gastroenterology 2008年第18期14卷 2810-2817页

作者： Peter Hafkemeyer Ulrich Brinkmann elizabeth Brinkmann Ira Pastan Hubert E Blum Thomas F Baumert Department of Medicine Ⅱ University Hospital Freiburg Hugstetterstrasse 55 Freiburg D-79106 Germany Roche Diagnostics Nonnenwald 2 Penzberg D-82377 Germany National Cancer Institute National Institutes of Health Bethesda United States Inserm unit 748 Université Louis Pasteur 3 Rue Koeberle Strasbourg F-67000 France

AIM: To present an approach for selectively killing retrovirus-infected cells that combines the toxicity of Pseudomonas exotoxin (PE) and the presence of reverse transcriptase (RT) in infected cells. METHODS: PE antisense toxin RNA has palindromic stem loops at its 5' and 3' ends enabling self-primed generation of cDNA in the presence of RT. The RT activity expressed in retrovirus-infected cells converts "antisense-toxin-RNA" into a lethal toxin gene exclusively in these cells. RESULTS: Using cotransfection studies with PE-expressing RNAs and β-gal expressing reporter plasmids, we show that, in HepG2 and HepG2.2.15 hepatoma cells as well as in duck hepatitis B virus (DHBV) infected cells, HBV or DHBV-polymerase reverse transcribe a lethal cDNA copy of an antisense toxin RNA, which is composed of sequences complementary to a PE gene and eukaryotic transcription and translation signals. CONCLUSION: This finding may have important implications as a novel therapeutic strategy aimed at the elimination of HBV infection.

关键词： Gene therapy Pseudomonas exotoxin Retrovirus Reverse transcription

Skits in English New ed.

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Survey of insect visitation of ornamental flowers in Southover Grange garden, Lewes, UK

1980年

作者： Hines Mary elizabeth.

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Insect Science 2015年第5期22卷 700-705页

作者： Mihail Garbuzov elizabeth E. W.samuelaon Francis L. W. Ratnieks Laboratory of Apiculture & Social Insects School of Life Sciences University of Sussex Falmer Brighton BN1 9QG United Kingdom

Ornamental flowers commonly grown in ttrban gardens and parks can be of value to flower-visiting insects. However, there is huge variation in the number of insects attracted among plant varieties. In this study, we quantified the insect attractiveness of 79 varieties in full bloom being grown in a public urban garden that is popular due to its beautiful flowers and other attractions. The results showed very clearly that most varieties （77%, n = 61） were either poorly attractive or completely unattractive to insect flower visitors. Several varieties （19%, n = 15） were moderately attractive, but very few （4%, n = 3） were highly attractive. Closer examination of Dahlia varieties showed that ＂open＂ flowered forms were approximately 20 times more attractive than ＂closed＂ flowered forms. These results strongly suggest that there is a great potential for making urban parks and gardens considerably more bee- and insect-friendly by selecting appropriate varieties.

关键词： bumble bees Dahlia double flowers honey bees pollinating insects wildlifefriendly gardening

Insights into remediation technology for malachite green wastewater treatment

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Water Science and Engineering 2023年第3期16卷 261-270页

作者： Peter Olusakin Oladoye Timothy Oladiran Ajiboye Wycliffe Chisutia Wanyonyi elizabeth Oyinkansola Omotola Mayowa Ezekiel Oladipo Department of Chemistry and Biochemistry Florida International UniversityMiami 33199USA Analytical/Environmental Chemistry Unit Department of Pure and Applied ChemistryLadoke Akintola University of TechnologyOgbomoso 210214Nigeria Department of Chemistry Nelson Mandela UniversitySummerstrand 6031South Africa Material Science Innovation and Modelling(MaSIM)Research Focus Area Faculty of Natural and Agricultural SciencesNorth-West UniversityMmabatho 2735South Africa Department of Mathematics Actuarial and Physical SciencesSchool of Science and TechnologyUniversity of KabiangaKericho 20201Kenya Department of Chemical Sciences Tai Solarin University of EducationIjebu Ode 120101Nigeria

Malachite green (MG) dye is a common industrial dye and organic contaminant that can be found in (waste)water. Textile and food industries make use of MG as dyeing and food coloring agents, respectively. However, MG is both genotoxic and mutagenic. Hence, the elimination of MG from MG-laden-wastewater is germane. This review summarizes up-to-date researches that have been reported in literature as regards the decontamination of toxic MG wastewater. Various removal methods (adsorption, membrane, Fenton system, and heterogenous and homogeneous photodegradation) were discussed. Of the two basic technologies that are comprehensively explored and reviewed, chemical treatment methods are not as viable as physical removal methods, such as the adsorption technology, due to the lack of secondary pollutant production, simple design, low operation costs, and resource availability. This review also presents various practical knowledge gaps needed for large-scale applications of adsorptive removal methods for MG. It concludes by recommending further research on the techniques of cheap and simple decontamination of MG to get clean water.

关键词： Contaminant Wastewater Decontamination Pollution Malachite green Dye

A prospective study of time to healing and hypertrophic scarring in paediatric burns:every day counts

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Burns & Trauma 2017年第1期5卷 44-49页

作者： elizabeth Chipp Lisa Charles Clare Thomas Kate Whiting Naiem Moiemen Yvonne Wilson Burns Centre Birmingham Children’s HospitalSteelhouse LaneBirminghamUK

Background: It is commonly accepted that burns taking longer than 3 weeks to heal have a much higher rate of hypertrophic scarring than those which heal more quickly. However, some of our patients develop hypertrophic scars despite healing within this 3-week period. Methods: We performed a prospective study of 383 paediatric burns treated non-operatively at a regional burns centre over a 2-year period from May 2011 to April 2013. Scar assessment was performed by a senior burns therapist using the Vancouver Scar Scale. Results: Overall rates of hypertrophic scarring were 17.2%. Time to healing was the strongest predictor of developing hypertrophic scarring, and the earliest hypertrophic scar developed in a patient who was healed after 8 days. The risk of hypertrophic scarring was multiplied by 1.138 for every additional day taken for the burn wound to heal. There was a trend towards higher rates of hypertrophic scarring in non-white skin types but this did not reach statistical significance. Conclusions: The risk of hypertrophic scarring increases with every day and, therefore, every effort should be made to get the wound healed as quickly as possible, even within the traditional 3-week period usually allowed for healing. We believe that the traditional dogma of aiming for healing within 3 weeks is overly simplistic and should be abandoned: in paediatric burns, every day counts.

关键词： Scar Hypertrophic Burn Time to healing Paediatric Skin type