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Chikungunya virus,epidemiology,clinics and phylogenesis:A review

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Asian Pacific Journal of Tropical Medicine 2014年第12期7卷 925-932页

作者： Alessandra Lo Presti Alessia Lai eleonora cella Gianguglielmo Zehender Massimo Ciccozzi Department of Infectious Parasitic and Immunomediated Diseases Epidemiology UnitReference Centre on Phytogeny Molecular Epidemiology and Microbial Evolution(FEMEM).Istituto Superiore di Sanita RomeItaly Department of Biomedical and Clinical Sciences L Sacco HospitalUniversity of MilanMilanItaly University Campus-Biomedico RomeItaly

Chikungunva virus is a mosquito-transmitted alphavirus that causes chikungunva fever,a febrile illness associated with severe arthralgia and *** virus is transmitted by culicine mosquitoes:Chikungunya virus replicates in the skin,disseminates to liver,muscle,*** tissue and brain,presumably through the *** studies showed that the Indian Ocean and the Indian subcontinent epidemics were caused by two different introductions of distinct strains of East/Central/South African genotype of *** paraphyletic grouping of African CHIK viruses supports the historical evidence that the virus was introduced into Asia from *** analysis divided Chikungunva virus isolates into three distinct genotypes based on geographical origins:the first,the West Africa genotype,consisted of isolates from Senegal and Nigeria;the second contained strains from East/Central/South African genotype,while the third contained solely *** most recent common ancestor for the recent epidemic,which ravaged Indian Ocean islands and Indian subcontinent in 2004- *** found to date in *** lineage dated about 1952 and exhibits similar spread patterns of the recent Indian Ocean outbreak lineage,with successive epidemics detected along an eastward *** group splitted into two clades:an Indian lineage and a south east *** of Chikungunya virus fever in Asia have not been associated necessarily with outbreaks in *** tools can reconstruct geographic spread of Chikungunva virus during the epidemics *** good management of patients with acute Chikungunva virus infection is essential for public health in susceptible areas with current Aedes spp activity.

关键词： CHIKV Epidemiology Phylogeny

The phylogenetic and evolutionary history of Kokobera virus

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Asian Pacific Journal of Tropical Medicine 2016年第10期9卷 945-950页

作者： Aletheia Blasi Alessandra Lo Presti eleonora cella Silvia Angeletti Massimo Ciccozzi Unit of Clinical Pathology and Microbiology University Campus Bio-Medico of Rome Department of Infectious Parasitic and Immunomediated Diseases Istituto Superiore di Sanità Public Health and Infectious Diseases Sapienza University

Objective: To estimate the genetic diversity of Kokobera virus, the date of origin and the spread among different viruses in the endemic regions of Australia. Methods: Two datasets were built. The first consisting of 29 sequences of the NS5/3' UTR region of Kokobera group downloaded from Gen Bank, the second including only 24 sequences of Kokobera viruses, focus is on this group. Results: Bayesian time analysis revealed two different entries in Australia of Kokobera virus in the 50 s years with the dated ancestor in 1861 year. Clade A and B showed a clear separation of the Kokobera sequences according to the geographic region. Conclusions: Data from the study showed as Kokobera virus, despite of its ancient origin and its circulation before the European colonization, remained limited to the Australian Country and nowadays limited mostly to the regions were Australian marsupials are mostly found.

关键词： Kokobera virus Phylogeny Date of origin Evolution

Phylogeny of Murray Valley encephalitis virus in Australia and Papua New Guinea

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Asian Pacific Journal of Tropical Medicine 2016年第4期9卷 376-380页

作者： eleonora cella Ivan Gabrielli Gianguglielmo Zehender Marta Giovanetti Alessandra Lo Presti Alessia Lai Giordano Dicuonzo Silvia Angeletti Marco Salemi Massimo Ciccozzi Department of Infectious Parasitic and Immunomediated DiseasesNational Institute of Health Department of Public Health and Infectious Diseases.Sapienza University of Rome University of Rome 'La Sapienza' L.Sacco Department of Biomedical and Clinical Sciences University of Milan University of Rome 'Tor Vergata' Clinical Pathology and Microbiology Laboratory University Hospital Campus Bio-Medico of Rome Department of Pathology and Laboratory Medicine.University of Florida University Hospital Campus Bio-Medico

Objective:To study the genetic diversity of Murray Valley encephalitis virus(MVEV) in Australia and Papua New ***:MVEV envelope gene sequences were aligned using Clustal X and manual editing was performed with *** v.3.7 was used to select the simplest evolutionary model that adequately fitted the sequence *** likelihood analysis was performed using *** phylogenetic signal of the dataset wa.s investigated by the likelihood mapping *** Bayesian phylogenetic tree was built using ***:The phylogenetic trees showed two main *** clade Ⅰincluding eight strains isolated from West *** clade Ⅱ was characterized by at least four epidemic entries,three of which localized in Northern West Australia and one in Papua New *** estimated mean evolutionary rate value of the MVEV envelope gene wa.s0.407 × 10^(-3) substitution/site/year(95%HPD:0.623 × 10~4-0.780× 10^(-3)).Population dynamics defines a relative constant population until the year *** a reduction occurred,probably due to a ***:This study has been useful in supporting the probable connection between climate changes and viral evolution also by the vector point of view:multidisciplinary monitoring studies are important to prevent new viral epidemics inside and outside new endemic areas.

关键词： Murray Valley encephalitis virus Phylogeny Evolution

Genetic diversity in Ebola virus:Phylogenetic and in silico structural studies of Ebola viral proteins

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Asian Pacific Journal of Tropical Medicine 2016年第4期9卷 329-335页

作者： Alba Grifoni Alessandra Lo Presti Marta Giovanetti Carla Montesano Massimo Amicosante Vittorio Colizzi Alessia Lai Gianguglielmo Zehender eleonora cella Silvia Angeletti Massimo Ciccozzi ProxAgen Ltd.Sofia.Bulgaria Department of Infectious Parasitic and Immunomediated Diseases National Institute of Health Department of Biology University of Rome 'Tor Vergata' Department of Biomedicine and Prevention University of Rome Tor Vergata Laboratory of Infectious Diseases and Tropical Medicine University of Milan Department of Public Health and Infectious Diseases.Sapienza University of Rome.Rome Clinical Pathology and Microbiology Laboratory University Hospital Campus Bio-Medico of Rome University Campus Bio-Medico

Objective:To explore the genetic diversity and the modification of antibody response in the recent outbreak of Ebola ***:Sequences retrieved from public databases,the selective pressure analysis and the homology modelling based on the all protein(***35,*** glycoprotein,small soluble ***30,VP24 and polymerase) were ***:Structural proteins ***35 and VP40 showed relative conserved sequences making them suitable target candidates for antiviral *** the contrary,*** and soluble glycoprotein have high mutation ***:Data from this study point out important aspects of Ebola virus sequence variability that for epitope and vaccine design should be considered for appropriate targeting of conserved protein regions.

关键词： Ebola virus Proteins Evolutionary analysis

Zika virus spreading in South America: evolutionary analysis of emerging neutralizing resistant Phe279Ser strains

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Asian Pacific Journal of Tropical Medicine 2016年第5期9卷 432-438页

作者： Marta Giovanetti Teresa Milano Luiz Carlos Alcantara Laura Carcangiu eleonora cella Alessia Lai Alessandra Lo Presti Stefano Pascarella Gianguglielmo Zehender Silvia Angeletti Massimo Ciccozzi Department of Infectious Parasitic and Immunomediated Diseases National Institute of HealthRomeItaly Department of Biology University of Rome ‘Tor Vergata’RomeItaly Laboratory of Hematology Genetic and Computational Biology Goncalo Moniz Research Center Oswaldo Cruz Foundation (LHGB/CPq GM/FIOCRUZ)SalvadorBahiaBrazil Dipartimento di Scienze Biochimiche ‘A. Rossi Fanelli’ Universita La Sapienza00185 RomeItaly Department of Public Health and Infectious Diseases Sapienza University of RomeRomeItaly Laboratory of Infectious Diseases and Tropical Medicine University of Milan Clinical Pathology and Microbiology Laboratory University Hospital Campus Bio-Medico of RomeRomeItaly University Campus Bio-Medico RomeItaly

Objective: To investigate the genetic diversity of Zika virus(ZIKV) and the relationships existing among these circulating viruses worldwide. To evaluate the genetic polymorphisms harboured from ZIKV that can have an influence on the virus circulation. Methods: Three different ZIKV dataset were built. The first dataset included 63 E gene sequences, the second one 22 NS3 sequences and the third dataset was composed of 108 NS5 gene sequences. Phylogenetic and selective pressure analysis was performed. The edited nucleic acid alignment from the Envelope dataset was used to generate a conceptual translation to the corresponding peptide sequences through UGene software. Results: The phylogeographic reconstruction was able to discriminate unambiguously that the Brazilian strains are belonged to the Asian lineage. The structural analysis reveals instead the presence of the Ser residue in the Brazilian sequences(however already observed in other previously reported ZIKV infections) that could suggest the presence of a neutralization-resistant population of viruses. Conclusions: Phylogenetic, evolutionary and selective pressure analysis contributed to improve the knowledge on the circulation of ZIKV.

关键词： Zika virus Phylogeny Evolution

Mayaro virus infection, the next epidemic wave after Zika?Evolutionary and structural analysis

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Asian Pacific Journal of Tropical Medicine 2018年第3期11卷 194-201页

作者： eleonora cella Marta Giovanetti Teresa Milano Marta Fogolari Francesco Garilli Ivailo Alexiev Riccardo Bazzardi Marco Salemi Luiz Carlos Junior Alcantara Silvia Angeletti Stefano Pascarella Massimo Ciccozzi Public Health and Infectious Diseases Sapienza University Unit of Clinical Laboratory Science University Campus Bio-Medico of Rome Fundao Oswaldo Cruz University of Rome"Tor Vergata" Dipartimento di Scienze Biochimiche"A.Rossi Fanelli" Universita La Sapienza Department of Medicine University Campus Bio-Medico of Rome National Reference Laboratory of HIV National Center of Infectious and Parasitic Diseases Department of Food Hygiene Experimental Zooprophylactic Institute of Sardinia Emerging Pathogens Institute Unirersity of Florida

Objective: To evaluate the evolution of the pathogen Mayaro virus, causing Mayaro fever(a mosquito-borne disease) and to perform selective pressure analysis and homology ***: Nine different datasets were built, one for each protein(from protein C to non-structural protein 4) and the last one for the complete genome. Selective pressure and homology modelling analyses were applied. Results: Two main clades(A and B) were pointed in the maximum likelihood tree. The clade A included five Brazilian sequences sampled from 1955 to 2015. The Brazilian sequence sampled in 2014 significantly clustered with the Haitian sequence sampled in 2015. The clade B included the remaining 27 sequences sampled in the Central and Southern America from 1957 to 2013. Selective pressure analysis revealed several sites under episodic diversifying selection in envelope surface glycoprotein El, non-structural protein 1 and nonstructural protein 3 with a posterior probability P≤0.01. Homology modelling showed different sites modified by selective pressure and some protein-protein interaction sites at high interaction propensity. Conclusion: Maximum likelihood analysis confirmed the Mayaro virus previous circulation in Haiti and the successful spread to the Caribbean and USA. Selective pressure analysis revealed a strong presence of negatively selected sites, suggesting a probable purging of deleterious polymorphisms in functional genes. Homology model showed the position 31, under selective pressure, located in the edge of the ADP-ribose binding site predicting to possess a high potential of protein-protein interaction and suggesting the possible chance for a protective vaccine,thus preventing Mayaro virus urbanization as with Chikungunya virus.

关键词： Mayaro virus Proteins Evolutionary analysis

Procalcitonin and mid-regional pro-adrenomedullin as promising markers for sepsis diagnosis and prognosis

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Asian Pacific Journal of Tropical Biomedicine 2016年第11期6卷 973-974页

作者： Silvia Angeletti Rossella Ottaviani Arcangela Michela Lanotte Lucia Pedicino Marina De Cesaris Alessandra Lo Presti eleonora cella Marta Fogolari Massimo Ciccozzi Giordano Dicuonzo Unit of Clinical Pathology and Microbiology Campus Bio-Medico University of Rome Department of Infectious Parasitic and Immunomediated Diseases Istituto Superiore di Sanità Public Health and Infectious Diseases Sapienza University

To the Editor,We read with great interest the recent review by Limongi et *** sepsis biomarkers[1].Recently,the combined use of two biomarkers,procalcitonin(PCT)and mid-regional proadrenomedullin(MR-pro ADM)has been reported in sepsis diagnosis and *** is a polypeptide produced as a precursor of calcitonin by thyroid C cells normally

关键词： PCT MR ADM than

Phylogeny of Culex theileri virus flavivirus in Spain, Myanmar, Portugal and Turkey

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Asian Pacific Journal of Tropical Medicine 2019年第5期12卷 216-223页

作者： eleonora cella Domenico Benvenuto Daniele Donati Francesco Garilli Silvia Angeletti Stefano Pascarella Massimo Ciccozzi Unit of Medical Statistics and Molecular Epidemiology,University Campus Bio-Medico of Rome School of Nursing,Faculty of Medicine,Department of Biomedicine and Prevention,Tor Vergata University Faculty of Medicine,University Campus Bio-Medico of Rome Unit of Clinical Laboratory Science,University Campus Bio-Medico of Rome Department of Biochemical Sciences'A.Rossi Fanelli',University of Rome'La Sapienza' Unit of Medical Statistics and Molecular Epidemiology University Campus Bio-Medico of Rome School of Nursing Faculty of Medicine Department of Biomedicine and Prevention Tor Vergata University Faculty of Medicine University Campus Bio-Medico of Rome Unit of Clinical Laboratory Science University Campus Bio-Medico of Rome Department of Biochemical Sciences"A.Rossi Fanelli" University of Rome "La Sapienza"

Objective: To study the genetic diversity of Culex theileri flavivirus and the spread of this virus among Spain, Portugal and ***: A database consisting of 55 sequences of the NS5/3'UTR region of Culex theileri flavivirus group downloaded from GenBank were aligned and manual edited with *** est v.3.7 was used to select the simplest evolutionary model that adequately fitted the sequence *** likelihood analysis was performed using *** phylogenetic signal of the dataset was investigated by the likelihood mapping ***: The phylogenetic tree showed three *** sequences clusterd together with Turkish sequences, Spain and Portugal strains grouped together and two Turkish sequences grouped *** pressure analysis showed a moderate percentage of sites(22.5%) under pervasive negative selection and only 1% under pervasive positive *** sites subject to selective pressure in CTFV RdRp NS5 fragments have been located onto the predicted three-dimensional ***: Phylogenetic and evolutionary analysis can be an important tool for understanding the evolutionary impact of the probable contemporary existence between nonpathogenic and pathogenic flaviviruses among these vectors.

关键词： Culex virus Flaviviridae Phylogenetic analysis Evolution

Assessment of slow deformations and landslide modelling in the urban area through a multi-methodological approach

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Journal of Earth Science 2024年

作者： Fabio Ietto Massimo Conforti Cristiano Tolomei Federico cella Giuseppe Cianflone Università degli Studi della Calabria， Dipartimento di Biologia， Ecologia e Scienze della Terra (DiBEST)， Via P.Bucci， Cubo 15B， 87036 Consiglio Nazionale Ricerche (CNR)， Istituto di Ricerca per la Protezione Idrogeologica (IRPI)， Via Cavour INGV-Istituto Nazionale di Geofisica e Vulcanologia， Via di Vigna Murata 605 Università di Camerino， Scuola di Scienze e Tecnologia， Sez.Geologia， Via Gentile III da Varano 27

Slow-moving landslides are widespread in the Mediterranean area， causing damage to the exposed facilities and economic losses in many countries. The recognition of slow-moving landslides in urban areas is always a difficult task to deal with because the presence of buildings， infrastructures， and human activities usually conceals the morphological signs of these landslide activities. So， in the last decades， numerous researchers have shown new methodologies to deepen the studies of similar instability phenomena. The present research is based on an integrated approach to investigate the landslide boundaries， type of movement， failure surface depth， and vulnerability state of buildings in Rota Greca village (Calabria region， southern Italy) affected by a slow-moving landslide. For this purpose， multi-source data were acquired through geological and geomorphological surveys， recognition of landslide-induced damage on the built environment， subsurface investigations (e.g.， continuous drill boreholes， Standard Penetration Test， Rock Quality Designation index and inclinometer monitoring)， laboratory tests (direct shear tests on undisturbed samples)， geophysical survey， and InSAR-derived map of deformation rates. The complete integration of multi-source data allowed for the construction of reliable landslide modelling with relative geotechnical properties. In addition， the cross-comparison between surface deformation data by SAR images and severity damage level collected on the exposed buildings enabled to obtain the vulnerability map of the built area. In particular， the achieved goals highlighted two failure surfaces at about -13 and -25 m depth， causing a high vulnerability value for the buildings allocated in the central portion of the Rota Greca village. The knowledge acquired by the multi-approach can be used to manage and implement appropriate landslide risk mitigation strategies， providing helpful advice and best practices to state-run organisations and stakeholder

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