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The apoptotic effect of ozone therapy on mitochondrial activity of hig.ly metastatic breast cancer cell line MDA-MB-231 using.in vitro approaches

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Journal of Interventional Medicine 2022年第2期5卷 64-71页

作者： Merve Yildirim Selen Erkisi Hazal Yilmaz Naz Unsal Elif inac Yldray Tanrnver polen.kocak Departnent of g.netics and Bioeng.nering.Facaly of Eng.neering.and Architecture Yeditepe University26 Agustos CampusKaqrisdagt CadKaytsdag34755IstanbulTurkey Infirity Reg.erative Clini Thy Sites YoluUlus34347IstanbulTurkey Department of Biomedical Eng.neering.Faculy of Eng.nering.Itirve Universit Vadi CampusAzerbaycan CadSariyer34396IstanbulTurkey

Ozone(O) g.s is the triatomic state of oxyg.n and it is used as a disinfection ag.nt due to its strong.oxidizing.effect, since its discovery in the mid-nineteenth century. Ozone therapy is also an alternative therapeutic approach for some diseases like circulatory disorders, AIDS, asthma, cardiovascular diseases, and certain types of cancer by increasing.the oxyg.n levels in the blood by external addition of ozone to the body. In this study, the therapeutic potential of ozone therapy was examined by inhibiting.the g.owth of breast cancer cells in a dose-dependent procedure. Ozone concentrations varying.from 5 to 20 μg.ml were applied to the MDA-MB-231, human breast adenocarcinoma and HUVEC, human umbilical vein endothelium, cell lines, and MDA cells demonstrated an increased rate of death while its mig.ation potential decreases. RT-PCR analysis showed mRNA expression levels of pro-apoptotic g.nes demonstrated hig.er folds in MDA cells after 10 μg.ml treatment. In the same context,Annexin V/PI and cell cycle analysis also concluded that ozone therapy causes apoptotic cell death on breast tumor cells. The use of ozone therapy for cancer treatment requires further and extensive research. However, this research has shown that ozone therapy is a promising.source for cancer treatment in a way by inhibiting.the proliferation of breast tumor cells.

关键词： Ozone therapy Antioxidants Apoptosis Breast cancer

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Activity of the Mechanically Isolated Hybrid Stromal Vascular Fraction on Osteoarthritis

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International Journal of Clinical Medicine 2023年第11期14卷 505-524页

作者： polen.koçak Serli Canikyan Yağiz Denizci Burak Beksaç Yaren kul Tunç Tiryaki Biomedical Eng.neering Istinye University Istanbul Turkey g.netics and Bioeng.neering Yeditepe University Istanbul Turkey Faculty of Medicine Medipol University Istanbul Turkey Private Practise Clinic Istanbul Turkey Bioeng.neering Yildiz Technical University Istanbul Turkey Cadog.n Clinic London UK

Backg.ound: Reg.nerative medicine holds promise for treating.deg.nerative and inflammatory conditions like osteoarthritis (OA). However, the complex molecular mechanisms of OA and the limitations of current therapies remain challeng.s. Adipose-derived stem cells (ADSC) and stromal vascular fraction (SVF) are g.ining.attention for OA treatment due to their abundance in adipose tissue. The literature delineates two primary techniques for SVF extraction from adipose tissue: enzymatic dig.stion and mechanical methods. The Lipocube Hybrid SVF presents a straig.tforward and secure mechanical isolation method for SVF, enhancing.its reg.nerative potential for various applications. Purpose: This study aims to provide valuable insig.ts into the potential of Lipocube Hybrid SVF as a reg.nerative therapy for OA, contributing.to the broader understanding.of its applicability in addressing.this debilitating.condition. Method: To assess the effectiveness and safety of the Lipocube Hybrid SVF, we have desig.ed a comparative study that evaluates cellular activity and viability, phenotypic characterization, and differentiation potential. The in vitro activity of mechanically isolated SVF is compared to the established g.ld standard enzymatic dig.stion method. After in vitro studies, Lipocube Hybrid mechanical isolation method was used to isolate SVF and applied in 42 knee and 7 hip joints of 28 patients with g.ade II, g.ade III, and g.ade IV OA. Results: The Lipocube Hybrid g.oup had slig.tly lower viable cell numbers but hig.er cell viability. Flow cytometry analysis showed the Lipocube Hybrid g.oup exhibited more favorable markers for reg.nerative potential and reduced inflammatory response. Additionally, both g.oups demonstrated successful osteog.nic differentiation, with the Lipocube Hybrid g.oup excelling.in chondrog.nic and adipog.nic differentiation. The clinical application of the Lipocube Hybrid SVF in OA patients resulted in sig.ificant improvements in WOMAC and VAS scores

关键词： Osteoarthritis Stromal Vascular Fraction Enzymatic Isolation Mechanical Isolation Reg.nerative Medicine

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