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Effects of reduced glutathione on Boer goat semen freezability

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Asian pacific Journal of Reproduction 2018年第1期7卷 33-38页

作者： rawash ZM Ebtihal A Ibrahim el-Raey M Artificial Insemination and Embryo Transfer Department Animal Reproduction Research InstituteAl Haram Biology of Reproduction Department Animal Reproduction Research InstituteAl Haram Department of Theriogenology Faculty of Veterinary MedicineBenha UniversityP.O:13736TokhKaliobiaEgypt

Objective: To evaluate the effects of reduced glutathione on the quality of cryopreserved Boer buck spermatozoa. Methods: The current study was carried out on five Boer bucks from which semen samples were collected by artificial vagina. After microscopical evaluation at 37 ℃, semen samples that fulfill the ideal requirements for extension were diluted in a tris–based extender including different concentrations of reduced glutathione (2, 5, 7 and 10 mM) and those without glutathione served as a control. Sperm motility, viability, acrosome integrity, DNA integrity, total antioxidant capacity and lipid peroxidation were assessed post-thawing. Results: The current results revealed that post-thawing motility, viability and acrosomal integrity were significantly improved [(66.67±5.50)%, 168.30±18.59 and (12.75±2.45)%, respectively] when 5 mM glutathione was added to semen extender;especially as compared with the control ((40.00 ±2.88)%, 95.00±8.90 and (25.75±3.46)%, respectively)Similarly, at this concentration (5 mM) sperm DNA damage, tail length and tail moment of cryopreserved semen were significantly (Pely] compared with the control extender ((6.66±0.84)%, (4.09±0.47) μm and 26.47±0.51, respectively)Moreover, addition of 5 mM glutathione to buck semen extender significantly (Pely)Conclusions: The addition of 5 mM glutathione to semen diluent improve freezability of Boer buck spermatozoa through DNA protection from deterioration and oxidative stress reduction. Moreover, 10 mM of glutathione exerts cytotoxic effects on Boer buck semen.

关键词： Glutathione Cryopreservation Buck DNA integrity Antioxidant

Soybean lecithin-based extender improves Damascus goat sperm cryopreservationand fertilizing potential following artificial insemination

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Asian pacific Journal of Reproduction 2019年第4期8卷 174-180页

作者： Mohamed Fathi rawash Zaher Dohreig Ragab Inas Gamal Assi Mohamed Eman Abu-el Naga Magdy Badr Department of Theriogenology Faculty of Veterinary MedicineCairo UniversityGizaEgypt Artificial Insemination and Embryo Transfer Department Animal Reproduction Research InstituteGizaEgypt Immunity Department Animal Reproduction Research InstituteGizaEgypt Pathology Department Animal Reproduction Research InstituteGizaEgypt 5Department of TheriogenologyFaculty of Veterinary MedicineAswan UniversityAswanEgypt

Objective: To study the effect of adding different concentrations of soybean lecithin in Tris based extender and to compare the results of Tris soybean lecithin extender with two commercial diluents (egg yolk-based: BullXcell and plant-based: OptiXcell) for Damascus goat sperm cryopreservation. Methods: The ejaculates from 4 mature male Damascus goats were obtained by using an artificial vagina. Semen samples were pooled and diluted in Tris extender supplemented with soybean lecithin at different concentrations of 1.5%, 3.0%, 6.0% and 10.0% to get better concentration to be used for further experiments, with a final concentration of 240×106 spermatozoa/mL. Semen samples were packed in straws (0.25 mL), frozen by using an automated system and stored in liquid nitrogen at -196 ℃ for 48 h. After thawing (37 ℃/30 s), the samples were evaluated for sperm quality parameters, including sperm motility, membrane integrity and acrosome integrity. Malondialdehyde concentration was estimated as a marker for lipid peroxidation. Based on the previous investigations, only Tris extender supplemented with 3.0% soybean lecithin (based on its positive results) was used versus BullXcell and OptiXcell for sperm ultrastructure evaluation and artificial insemination by using electron microscope and artificial insemination of the synchronized does. Results: There was no significant difference between Tris-soybean lecithin at 3.0% and BullXcell/OptiXcell diluents in post-thaw sperm parameters and fertility following artificial insemination;meanwhile, the other concentrations of soybean lecithin (1.5%, 6.0% and 10%) showed lower sperm parameters following cryopreservation. Conclusions: Using of Tris-soybean lecithin based extender at the level of 3.0% can be an appropriate alternative to either BullXcell or OptiXcell for Damascus goat sperm cryopreservation.

关键词： Damascus goat Soy lecithin Sperm cryopreservation BullXcell OptiXcell

Influence of butylated hydroxytoluene addition to cryodiluents on freezability and DNA integrity of Boer and Zaraibi buck spermatozoa

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Asian pacific Journal of Reproduction 2020年第2期9卷 96-103页

作者： Ahmed RMel-Khawagah Zaher Mrawash Diya Ael-Badry Mohamed MMKandiel Theriogenology Department Faculty of Veterinary MedicineBenha UniversityEgypt Artificial Insemination and Embryo Transfer Department Animal Reproduction Research InstituteAgriculture Research CenterGizaEgypt

Objective:To evaluate the cryoprotective effect of butylated hydroxytoluene on buck frozen ***:Semen was collected from Boer(n=6)and Zaraibi(n=6)bucks by electroejaculator for 5 *** aliquots were diluted at 38℃in Tris-buffer with egg yolk 15.0%(vol/vol)(Tris-egg yolk extender)or soya lecithin 2.5%(weight/vol)(Tris-soya lecithin extender)supplemented with butylated hydroxytoluene at 0.0(as the control),0.5,1.0,2.0 and 4.0 ***-thawing motility(at 400×magnification),plasma(hypo-osmotic swelling test),acrosome(Trypan blue/Giemsa dual staining)membranes,DNA(comet assay),and lipid peroxidation(by malondialdehyde concentration)were ***:Spermatozoa motility was enhanced by butylated hydroxytoluene in Tris-soya lecithin extender at 0.5 mM in the two breeds,and in Tris-egg yolk extender at 1.0 mM in Boer and at 2.0 mM in Zaraibi bucks for up to 3 h *** and acrosome membranes and DNA integrity of the two breeds were maximally high with butylated hydroxytoluene at 1.0-2.0 mM in Tris-egg yolk extender and at 0.5-1.0 mM in Tris-soya lecithin *** peroxidation was minimal with butylated hydroxytoluene at 1.0-2.0 mM in Tris-egg yolk and soya lecithin extenders in the two *** hydroxytoluene at 4.0 mM deteriorated spermatozoa motility,and plasma and acrosome ***:The consequence of butylated hydroxytoluene on buck frozen-thawed spermatozoa varies with the levels of supplementation,buck breed,and phospholipid source in the *** parameters of Boer buck are better in their response to butylated hydroxytoluene than Zaraibi *** hydroxytoluene at 1.0 and 2.0 mM in Tris-egg yolk extender,and at 0.5 mM in Tris-soya lecithin extender represents the best concentrations and profitably improves the semen quality of buck semen.

关键词： Butylated hydroxytoluene Buck DNA Frozen semen Lipid peroxidation

Using nanotechnology to prevent fines migration while production

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Petroleum 2021年第2期7卷 168-177页

作者： Mohamed Mansour Mohamed eleraki Ahmed Noah el-Abbas Moustafa Faculty of Science Zagazig UniversityEgypt Egyptian Petroleum Research Institute(EPRI) Egypt The British University Egypt

Formation damage due to fines migration is a major reason for well productivity decline for oil and gas *** fines are small enough to pass through pore throats causing pore plugging and permeability *** factors affect fines migration such as flow rate,salinity,pH value,reservoir temperature and oil polarity,as well as changes in chemical environment induced by Enhanced Oil Recovery(EOR)*** paper focuses on the effect of flow rates on fines detachment from the grain surfaces,which causing permeability *** the fluid inside the reservoir moves towards the wellbore,the fluid velocity increases,when the fluid reaches the critical flow rate these fines can be picked up into the *** fines captured by thinner pore throats causing pore plugging and permeability *** concentrations of nanoparticles were used to fix these fines on their sources and prevent their mobilization at high flow *** unique technique used in this study is changing the potential surfaces between fines and grain surfaces to prevent fines movement above the critical flow ***_(2)and MgO NPs used in this study can be adsorbed on the pore surfaces and reduce the repulsion forces between fines and pore ***_(2) and MgO nanoparticles at different concentrations(0.25,0.50 and 0.75 g/L)were used on treating the Abu-rawash sandstone reservoir using Formation Damage System Cell *** experimental studies showed that using MgO NPs would prevent fines detachment from the pore surfaces and decrease the reduction of permeability at high flow rates more than SiO_(2) *** optimum concentration of MgO NPs was at 0.5 g/L as the permeability remediation at this concentration reaches to 64.83%.

关键词： Formation damage Fines migration Nanoparticles Zeta potential Permeability reduction Fluid flow rate

Yield Attributes and Oil Safety in the Hyperaccumulator Canola Plant Grown in a Bioremediated Sewaged Soil

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Journal of Agricultural Science and Technology(A) 2013年第12期3卷 1010-1016页

作者： Hussein Fawzy Abouziena Mohamed Saber Essam Hoballah Soad el-Ashry Alaa Mohamed Zaghloul Department of Botany Agricultural & Biological Research Division National Research Center Cairo 12622 Egypt Department of Agric. Microbiology Agricultural & Biological Research Division National Research Center Cairo 12622 Egypt Department of Soil and Water Use Agricultural & Biological Research Division National Research Center Cairo 12622 Egypt

A field experiment was carried out at Abu-rawash sewage farm to appraise the effect of certain novel remediative amendments on the quality of oil as well as the vegetative parameters and yield criteria of canola plant used as hyperaccumulator for the remediation of sewaged soils. The treatments included fallow soil （irrigated without growing canola）, soil cultivated with canola （Brassica napus L.） and inoculated with arbuscular mycorrhiza （AM）, soil inoculation with Thiobacillus sp. （a mixture of Thiobacillus ferrooxidans and Thiobacillus thiooxidant）, soil treated with a mixture of 250 mg bentonite plus 250 mg rock phosphate/kg soil and inoculated with phosphate dissolving bacteria （PDB）, and soil treated with all the aforementioned remediative amendments. Results indicated that the vegetative parameters and yield criteria of canola plant did not exhibit any serious adverse impact under all treatments applied. The concentrations of Zn and Cu in canola oil extracted from plants grown in soil inoculated with AM and/or Thiobacillus sp. far exceeded the safe permissible levels. On the other hand, the content of both PTEs in the oil extracted from canola plants grown in soil treated with either probentonite or with mixture of all remediative amendments followed the permissible safe levels.

关键词： Canola bioremediation of sewaged soil permissible levels of PTEs in canola oil phytoremediation Thiobacillus sp.,arbuscular mycorrhiza.