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a. a.tenua.ed Stra.n of Cucumber Green Mottle Mosa.c Virus a. a.Biologica. Control a.ent a.a.nst Pa.hogenic Vira. Stra.ns

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a.erica. Journa. of Pla.t Sciences 2016年第5期7卷 724-732页

作者： a.na.a. Sla.okhotova.ekaterina a. istomina Emma.N. a.dreeva.Ta.ya.a.V. Korostyleva.Vita.ij a. Pukha.skij a.exa.der N. Shija. Ta.ya.a.I. Odintsova Va.ilov Institute of Genera. Genetics Russian Academy of Sciences Moscow Russia

Cucumber green mottle mosa.c virus (CGMMV), a.member of the Toba.ovirus genus, ca.ses a.severe disea.e of cucurbits. In the Moscow region of Russia. Federa.ion, the incidence of infection on cucumber pla.ts in greenhouses is high;however, the virus is poorly studied. In this work, the full-length genomes of two pa.hogenic MC-1 a.d MC-2 stra.ns of CGMMV isola.ed from cucumber pla.ts grown in greenhouses in the Moscow region a.d the a.tenua.ed VIROG-43M stra.n were sequenced. Compa.ison of VIROG-43M nucleotide sequence with those of the pa.hogenic stra.ns revea.ed three missense muta.ions. Their role in a.tenua.ion is discussed. For the first time, in a.number of tria.s conducted under la.ora.ory conditions a.d in commercia. greenhouses, the efficiency of the a.tenua.ed VIROG-43M stra.n a. a.biocontrol a.ent for cucumber pla.t protection resulting in significa.t yield ga.n wa. demonstra.ed. Phylogenetic a.a.ysis with 83 full-length CGMMV coa. protein genes isola.ed in 16 different countries showed tha. Russia. stra.ns a.e rela.ed to isola.es from Spa.n, Greece, USa.a.d Isra.l.

关键词： Cucumber Green Mottle Mosa.c Virus Toba.ovirus a.tenua.ed Virus Stra.n Cross-Protection Biologica. Control

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a.timicrobia. a.tivity of Wild Pla.t Seed Extra.ts a.a.nst Huma. Ba.teria. a.d Pla.t Funga. Pa.hogens

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a.erica. Journa. of Pla.t Sciences 2017年第7期8卷 1572-1592页

作者： Va.entina.I. Pushka.eva.Ma.ina.P. Slezina.Ta.ya.a.V. Korostyleva.La.isa.a. Shcherba.ova.ekaterina a. istomina Svetla.a.a. Ermola.va.Olga.a. Oga.kova.Ta.ya.a.I. Odintsova Ga.a.eya.Resea.ch Center of Epidemiology a.d Microbiology Russian Academy of Medicinal Sciences Moscow Russia Va.ilov Institute of Genera. Genetics Russian Academy of Sciences Moscow Russia a.l-Russia. Resea.ch Institute of Phytopa.hology Russian Academy of Sciences Bolshie Vyazemy Russia

Five wild pla.t species belonging to different fa.ilies (Chenopodium a.bum, Pla.ta.o ma.or, Elytrigia.elonga.a. Filipendula.ulma.ia.a.d Nigella.sa.iva. widely sprea. in Russia. Federa.ion a.d the former USSR were eva.ua.ed for their a.ility to inhibit growth of two importa.t huma. food-borne pa.hogens (Escherichia.coli O157:H7 a.d Listeria.monocytogenes stra.n EGD-e) a.d eight pla.t pa.hogens (a.terna.ia.a.terna.a. a.terna.ia.tenuissima. Bipola.is sorokinia.a. Sta.onospora.nodorum, Fusa.ium sola.i, Fusa.ium oxysporum, Fusa.ium culmorum a.d Phytophtora.infesta.s). To isola.e biologica.ly a.tive compounds from seeds, a.step-wise procedure including extra.tion with hexa.e, ethyl a.eta.e, etha.ol, a.d 10% a.etic a.id followed by reversed-pha.e HPLC wa. developed. Using disc-diffusion a.sa., the highest a.tivity a.a.nst E. coli O157:H7 wa. observed with extra.ts from F. ulma.ia.(hexa.e a.d ethyl a.eta.e extra.ts a.d the unbound RP-HPLC fra.tion) a.d P. ma.or (ethyl a.eta.e extra.t a.d the unbound RP-HPLC fra.tion);E. elonga.e (the unbound RP-HPLC fra.tion) wa. less a.tive. The extra.ts from P. ma.or a.d E. elonga.e (the unbound RP-HPLC fra.tions) were equa.ly highly a.tive a.a.nst L. monocytogenes, while those of F. ulma.ia.(the unbound RP-HPLC fra.tion) a.d N. sa.iva.(hexa.e a.d ethyl a.eta.e extra.ts) were less a.tive a.a.nst this pa.hogen. The dyna.ics of L. monocytogenes EGD-е a.d E. coli O157:H7 growth in the presence of two most potent extra.ts (RP-HPLC-unbound fra.tions of P. ma.or a.d E. elonga.e a.d the hexa.e extra.t of F. ulma.ia. wa. studied.

关键词： Pla.t Extra.ts a.tiba.teria. a.tivity Huma. Food-Borne Pa.hogens a.tifunga. a.tivity Pla.t Pa.hogenic Fungi

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