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Therapy with bone marrow cells reduces liver alterations in mice chronically infected by Schistosoma mansoni

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World Journal of Gastroenterology 2008年第38期14卷 5842-5850页

作者： Sheilla Andrade Oliveira Bruno Solano Freitas souza Carla Adriana Guimares-Ferreira Elton Sá Barreto Siane Campos souza Luiz Antonio Rodrigues Freitas Ricardo Ribeiro-dos-Santos Milena Botelho Pereira soares Centro de Pesquisas Gonalo Moniz Fundao Oswaldo Cruz Rua Waldemar Falco 121 Brotas 40295-001 Salvador BA Brazil Centro de Pesquisas Aggeu Magalhes Fundao Oswaldo Cruz Recife Av Moraes Rego s/n Cidade Universitária Recife 50670-420 PE Brazil Centro de Pesquisas Gonalo Moniz Fundao Oswaldo Cruz Rua Waldemar Falco 121 Brotas Salvador 40295-001 BA Brazil Centro de Pesquisas Gonalo Moniz Fundao Oswaldo Cruz Rua Waldemar Falco 121 Brotas 40295-001 Salvador BA Brazil Hospital So Rafael Av So Rafael 2152 So Marcos Salvador 41256-900 BA Brazil

AIM： To investigate the potential of bone marrow mononuclear cells （BM-MCs） in the regeneration of hepatic lesions induced by Schistosoma mansoni （***） chronic infection. METHODS： Female mice chronically infected with *** were treated with BM-MCs obtained from male green fluorescent protein （GFP） transgenic mice by intravenous or intralobular injections. Control mice received injections of saline in similar conditions, Enzyme-linked immunosorbent assay （ELISA） assay for transforming growth factor-beta （TGF-β）, polymerase chain reaction （PCR） for GFP DNA, immunofluorescence and morphometric studies were performed. RESULTS： Transplanted GFP^＋ cells migrated to granuloma areas and reduced the percentage of liver fibrosis. The presence of donor-derived cells was confirmed by Fluorescence in situ hybridization （FISH） analysis for detection of cells bearing Y chromosome and by PCR analysis for detection of GFP DNA. The levels of TGF-β, a cytokine associated with fibrosis deposition, in liver fragments of mice submitted to therapy were reduced. The number of oval cells in liver sections of ***-infected mice increased 3-4 fold after transplantation. A partial recovery in albumin expression, which is decreased upon infection with ***, was found in livers of infected mice after cellular therapy. CONCLUSION： In conclusion, transplanted BMCs migrate to and reduce the damage of chronic fibrotic liver lesions caused by ***.

关键词： Schistosoma mansoni Liver injury Fibrosis Bone marrow cells Transplantation Albumin Oval cells

Biofilm analyses and exoproduct release by clinical and environmental isolates of Burkholderia pseudomallei from Brazil

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Asian Pacific Journal of Tropical Medicine 2023年第7期16卷 321-328页

作者： Glaucia Morgana de Melo Guedes Crister JoséOcadaque Alyne soares Freitas Rodrigo Machado Pinheiro Giovanna Barbosa Riello Silviane Praciano Bandeira Rossana de Aguiar Cordeiro Marcos Fábio Gadelha Rocha JoséJúlio Costa Sidrim Débora de souza Collares Maia Castelo-Branco department of Pathology and Legal Medicine Postgraduate Program in Medical MicrobiologyGroup of Applied Medical MicrobiologyFederal University of CearáFortalezaCEBrazil department of Clinical and Toxicological Analysis School of PharmacyFederal University of CearáFortalezaCEBrazil department of Pathology and Legal Medicine Postgraduate Program in Medical MicrobiologyLaboratory of Emerging and Reemerging PathogensFederal University of CearáFortalezaCEBrazil School of Veterinary Medicine Postgraduate Program in Veterinary SciencesState University of CearáFortalezaCEBrazil

Objective:To characterize biofilm production by clinical(n=21)and environmental(n=11)isolates of Burkholderia pseudomallei and evaluate the production of proteases,hemolysins and ***:Initially,the 32 strains were evaluated for biofilm production in Müller-Hinton broth-1%glucose(MH-1%glucose)and BHI broth-1%glucose,using the crystal violet staining ***,growing(48 h)and mature(72 h)biofilms were evaluated by confocal ***,the production of proteases,hemolysins and siderophores by planktonic aggregates,growing biofilms and mature biofilms was ***:All isolates produced biofilms,but clinical isolates had significantly higher biomass in both MH-1%glucose(Pderophores,mature biofilms presented higher production than growing biofilms(Pderophores seem important for growth and maintenance of Burkholderia pseudomallei biofilms.

关键词： Mangosteen Burkholderia pseudomallei Biofilms Proteases Hemolysins Siderophores

development and Characterization of Nanovesicles Containing Phenolic Compounds of Microalgae Spirulina Strain LEB-18 and Chlorella pyrenoidosa

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Advances in Materials Physics and Chemistry 2014年第1期4卷 6-12页

作者： Leticia Marques de Assis Adriana Rodrigues Machado Amanda de souza da Motta Jorge Alberto Vieira Costa Leonor Almeida de souza-soares School of Chemistry and Food Federal University of Rio GrandeRio GrandeBrazil Federal Institute Sul-Rio-Grandense Campus PelotasVisconde da GracaPelotasBrazil Institute of Basic Health Sciences Federal University of Rio Grande do SulPorto AlegreBrazil

The objective of this study was to elaborate liposomes, through the lipid film hydration methodology, to nanoencapsulate phenolic compounds of Spirulina LEB-18 and Chlorella pyrenoidosa microalgae, and evaluate their physicochemical characteristics and storage stability for 21 days. The total phenolic compounds were evaluated using a calibration curve of gallic acid using methanol and ethanol as extraction solvents. The size and polydispersity index of nanovesicles were determined by light scattering and the percentage encapsulation efficiency was determined by a centrifugation process. The stability of the liposomes at storage time was measured by zeta potential for 21 days. The methanol extracts from Spirulina had a higher content of phenolic compounds (2.62 mg gallic acid·g-1 of microalgae) compared to the extracts of Chlorella. However, liposomes with ethanolic extracts of the two algae showed higher encapsulation efficiency. The value was higher (96.40%) for Chlorella. All samples obtained nanometric size, with the highest value obtained for the liposome containing ethanol extract of Chlorella (239 nm) differing significantly (p ≤ 0.05) from the others. The liposomes containing extracts of Spirulina were more stable during the 21 days of storage, whereas, those consisting of ethanol extract showed no significant difference (p ≤ 0.05) throughout this period.

关键词： Microalgae Phenols Encapsulation Liposome

Distribution of polychaetes in the shallow, sublittoral zone of Admiralty Bay, King George Island, Antarctica in the early and late austral summer

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Natural Science 2010年第10期2卷 1155-1163页

作者： Letícia de souza Barbosa Abílio soares-Gomes Paulo Cesar Paiva 不详

This study assessed the spatial distribution pa- ttern of soft-sediment polychaetes on the near-shore of Admiralty Bay, King George Island, Antarctica. In the early and late summer of 2003 /04, seven sites at three different depths (20,30 and 60 meters) were sampled using a van Veen grab. 8,668 individuals all told, belonging to 67 species and 23 families, were identified. The families Terebellidae, Syllidae and Maldanidae were the most speciose. Mean densities ranged from 45.2 to 388.1 ind. 0.1 m-2 in the early summer, and from 29 to 183 ind.0.1m-2 in the late. The species Aphelochaeta cincinnata, Levinsenia gracilis and Rhodine antarctica were the most frequent and abundant. Initially, mean biomass ranged from 0.11 to 5.27 g.0.1 m-2, in the early season and from 0.35 to 5.86 g.0.1 m-2 towards the end. Aglaophamus trissophyllus, Eupolymnia sp. and Barrukia cristata were the species with the highest biomass. Polychaete taxocoenosis structure remained similar in both periods. In the early summer, mean densities, biomass and number of species were lower at 30 meters and higher at 60, whereas in the late, these differences were higher among transects. Ice impacts, mainly anchor-ice, in the early summer, as well as icebergs later on, most likely caused the differences encountered.

关键词： Polychaeta Soft-Sediment Benthic Structure South Shetland Islands Antarctic Peninsula

Analytical determination of Benzophenone-3 in Sunscreen Preparations Using Boron-Doped Diamond Electrodes

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American Journal of Analytical Chemistry 2011年第3期2卷 383-391页

作者： Michelli Thomaz Laranjeira Fabio de Lima Silvio Cesar de Oliveira Valdir souza Ferreira Robson Tadeu soares de Oliveira 不详

A new electroanalytical procedure was developed for the determination of Benzophenone-3 (BENZO) in commercial sunscreen as the active ingredient. The procedure is based on the use of electrochemical methods as cyclic and square-wave voltammetry, with boron-doped diamond (BDD) electrodes. The reduction of BENZO in Britton-Robinson buffer (0.1 mol●L–1) using this type of electrode gives rise to one irreversible peak in –1.30 V (versus Ag/AgCl) in presence of cationic surfactant cetyltrimethylammonium bromide (CTABr). The proposed electrochemical method was successfully applied to the analysis of commercially available pharmaceutical preparations.

关键词： Boron-Doped Diamond Benzophenone-3 BENZO and Square-Wave Voltammetry

The Current and Future Role of Drugs and Probiotics in the Management of Inflammatory Bowel Disease

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Journal of Biosciences and Medicines 2015年第8期3卷 76-85页

作者： Maricelma da Silva soares de souza Sandra Maria Barbalho Ricardo de Alvares Goulart Antonely de Cássio Alves de Carvalho department of Biochemistry and Pharmacology School of Medicine University of Marília Marília S&atilde o Paulo Brazil department of Biochemistry and Pharmacology School of Medicine University of Marília and Food Technology School (FATEC) Marília S&atilde o Paulo Brazil department of Gastroenteology University Hospital-UNIMAR Marília S&atilde o Paulo Brazil Diagnostic Center in Gastroenterology Ibaiti Paraná Brazil

Inflammatory Bowel Disease (IBD) and other auto-inflammatory disorders are conditions caused by chronic and persistent intestinal inflammation, which is mainly represented by Ulcerative Colitis (UC) or Crohn’s Disease (CD), both of which typically arise as chronic inflammation of the gastrointestinal mucosa. Doctor’s main focus for treating IBD is the decrease on inflammation processes when the disease is active and promotes the maintenance of inflammation at normal levels. However, to deal with these two parameters is not easy, because the drugs usually used are related to several side effects and they are not totally effective to reduce inflammation and to keep the remission. Authors agree that the therapy approach should include drugs, dietary intervention and use of alternative substances as probiotics. The standard treatment approach for patients with CD and UC is traditionally done with the use of anti-inflammatory agents, like corticosteroids, mesalamine, immunomodulators such as azathioprine and methotrexate, and biologic agents such as infliximab and others. Probiotics are known as viable micro-organisms (or live microorganisms or live microbial dietary supplements) that may promote beneficial physiologic or therapeutic properties when used correctly. The interest in the role of these substances in improving human health is the reason of several researches. They may be used to help the immune system, to control gastro-intestinal infections and to prevent several diseases. Prebiotics, symbiotics, and probiotics may be helpful as adjuvants in the treatment of IBD. Probiotics are normally well-tolerated and are associated with few side effects which are undoubtedly important in the treatment of any disease.

关键词： Inflammatory Bowel Diseases Immunomodulators Biological Agents Probiotics

Novel Protease from Aspergillus tamarii URM4634: Production and Characterization Using Inexpensive Agroindustrial Substrates by Solid-State Fermentation

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Advances in Enzyme Research 2016年第4期4卷 125-143页

作者： Osmar soares da Silva Rodrigo Lira de Oliveira Cristina Maria souza-Motta Ana Lúcia Figueiredo Porto Tatiana souza Porto Northeast Biotechnology Network/RENORBIO Federal Rural University of Pernambuco (UFRPE) Recife Brazil Academic Unity of Garanhuns/UAG Federal Rural University of Pernambuco (UFRPE) Garanhuns Brazil department Mycology Federal University of Pernambuco (UFPE) Recife Brazil department of Morphology Physiology Animal/DMFA Federal Rural University of Pernambuco (UFRPE) Recife Brazil

This study reports the protease production from Aspergillus tamarii using agroindustrial residues as substrate for solid-state fermentation (SSF) and biochemical characterization. The highest protease production was obtained using wheat bran as substrate at 72 h fermentation with maximum proteolytic activity of 401.42 U/mL, collagenase of 243.0 U/mL and keratinase of 19.1 U/mL. The protease exhibited KM = 18.7 mg/mL and Vmax = 28.5 mg/mL/min. The optimal pH was 8.0 and stable in a wide pH range (5.0 - 11.0) during 24 h. The optimum temperature was 40°C. The proteolytic activity was inhibited by Cu2+ (33.98%) and Hg2+ (22.69%). The enzyme was also inhibited by PMSF (65.11%), indicating that is a Serine Protease. These properties suggest that alkaline protease from A. tamarii URM4634 is suitable for application in food industries and leather processing. Additionally, the present findings opened new vistas in the utilization of wheat bran and other effective agroindustrial wastes as substrates for SSF.

关键词： Protease Aspergillus tamarii Biochemical Characterization Solid-State Fermentation Agroindustrial Waste

The first case report of Leishmania(leishmania) chagasi in Panthera leo in Brazil

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Asian Pacific Journal of Tropical Biomedicine 2011年第3期1卷 249-250页

作者： Magyda AA Dahroug Arleana BPF Almeida Valéria RF Sousa Valéria Dutra Luciana D Guimares César E soares Luciano Nakazato Roberto L de souza Federal University of Mato Grosso Ar.Fernando Correa da Costa s/n78060-900CuiabáMTBrasil

We reported here the first known case of natural infection of a lion(Panthera leo-Linnaeus,1738) with Leishmania(Leithmania) chagasi(***) in *** specimen was created by a circus handler in the state of Mato Grosso and was donated to the zoological park of the Federal University of Mato *** by *** was detected using a PCR-RFLP *** was known that the domestic felids can act as reservoir of infection of *** in endemic areas, making it important that studies demonstrate their participation in the epidemiological chain. We demonstrate in this work that wild animals can have an important role in the epidemiological chain and must be considered in order to plan methods of control of this zoonosis.

关键词： Panthera leo Leishmania(leishmania) chagasi PCR-RFLP

Dynamic Modeling and Experimental Damping Identification of a Flexible Structure

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材料科学与工程（中英文A版） 2013年第7期3卷 505-509页

作者： Marcelo Rodolfo de Assis Alvaro Manoel de souza soares JoAo Bosco Goncalves Pedro Paulo Leite do Prado Mechanieal Engineering department University of Taubate Campus da Juta Taubatd 12.060-440 SP Brazil Eletrical Engineering department University of Taubatd Campus da Juta Taubatd 12.060-440 SP Brazil