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Diagnosis of West Nile virus infections:Evaluation of different laboratory methods

World Journal of Virology 2024年第4期13卷 51-61页

作者： Tatjana Vilibic-Cavlek Maja Bogdanic Vladimir Savic Zeljka Hruskar Ljubo Barbic Vladimir Stevanovic Ljiljana Antolasic Ljiljana Milasincic Dario Sabadi Gorana Miletic Ivona Coric Anna Mrzljak eddy listes Giovanni Savini Department of Virology Croatian Institute of Public HealthZagreb 10000Croatia School of Medicine University of ZagrebZagreb 10000Croatia Poultry Center Croatian Veterinary InstituteZagreb 10000Croatia Department of Microbiology and Infectious Diseases with Clinic Faculty of Veterinary Medicine University of ZagrebZagreb 10000Croatia Department of Infectious Diseases Clinical Hospital Center OsijekOsijek 31000Croatia Medical Faculty Josip Juraj Strossmayer University of OsijekOsijek 31000Croatia Department of Gastroenterology and Hepatology University Hospital Center ZagrebZagreb 10000Croatia Croatian Veterinary Institute Veterinary Institute SplitSplit 21000Croatia OIE Reference Center for West Nile Disease Istituto Zooprofilattico SperimentaleG.CaporaleTeramo 64100Italy

BACKGROUND The diagnosis of West Nile virus(WNV)is challenging due to short-term and low-level viremia,flavivirus cross-reactivity,and long immunoglobulin M(IgM)*** To evaluate different methods for WNV detection[reverse transcription-polymerase chain reaction(RT-PCR),IgM/IgG antibodies,IgG avidity]in serum,cerebrospinal fluid(CSF),and urine samples of patients with confirmed WNV *** The study included patients with confirmed WNV neuroinvasive infection(n=62),asymptomatic WNV seropositive individuals(n=22),and individuals with false-positive WNV IgM antibodies(n=30).WNV RNA was detected using RT-PCR.A commercial ELISA was used to detect WNV IgM/IgG antibodies with confirmation of cross-reactive samples using a virus neutralization test(VNT).IgG-positive samples were tested for IgG *** The WNV-RNA detection rates were significantly higher in the urine(54.5%)/serum(46.4%)than in CSF(32.2%).According to the sampling time,the WNV-RNA detection rates in urine collected within 7 days/8-14/≥15 days were 29.4/66.6/62.5%(P=0.042).However,these differences were not observed in the *** median RT-PCR cycle threshold values were significantly lower in urine(32.5,IQR=28-34)than in CSF(34.5,IQR=33-36).The frequency of positive WNV IgM and IgG significantly differed according to the sampling time in serum but not in *** IgM/IgG antibodies were detected in 84.3/9.3%of serum samples collected within 7 days,100/71.1%of samples collected 8-14,and 100%samples collected after≥15 *** WNV infection was confirmed by low/borderline avidity index(AI)in 13.6%of asymptomatic individuals.A correlation between ELISA and AI was strong negative for IgM and strong positive for *** significant correlation between ELISA IgG and VNT was *** The frequency of WNV RNA and antibody detection depends on the sampling time and type of clinical *** avidity could differentiate recent WNV infections from long-persisting IgM antibo

关键词： West Nile virus Reverse transcription-polymerase chain reaction Serology IgG avidity Cross-reactivity

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