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过滤与分离 1999年第3期9卷 19-21页

作者：石秀东无锡轻工大学机械系江苏无锡214036

本文分析固液超声分离的原理，分析了两个平面声波在液体中如何产生移动驻波，依赖此移动特点把固体粒子移动到装置的一侧，然后分流，从而得到固液分离的目的。进一步分析了超声分离对所用超声换能器的频率、声强的要求。

关键词：固液分离超声波声强频率

抗甲肝病毒基因工程单抗分离纯化与鉴定(英文)

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生物工程学报 2004年第2期20卷 257-261页

作者：魏敬双陶苒孙巍巍贾茜李川梁米芳华北制药集团新药研究开发有限责任公司石家庄050015 中国疾病预防控制中心北京100052

为克服血源免疫球蛋白制品的不足 ,开发了抗甲肝病毒基因工程单克隆抗体anti_HAVIgG。用无血清培养基培养rCHO工程细胞株 ,上清液经过rProteinASFF亲和层析→脱盐→离子交换层析→超滤换液纯化后 ,所得anti_HAVIgG纯度达 99%以上 ,比活... 详细信息

为克服血源免疫球蛋白制品的不足 ,开发了抗甲肝病毒基因工程单克隆抗体anti_HAVIgG。用无血清培养基培养rCHO工程细胞株 ,上清液经过rProteinASFF亲和层析→脱盐→离子交换层析→超滤换液纯化后 ,所得anti_HAVIgG纯度达 99%以上 ,比活性约 10 0IU mg ,anti_HAVIgG活性回收率 40 %。所纯化的anti_HAVIgG分子量150kd ,等电点 8 4～ 9 3。免疫印迹实验证实anti_HAVIgG为人源全抗体分子。亲和层析介质rProteinASFF确实存在亲和配基脱落问题 ,但通过后续纯化步骤可有效除去。在亲和层析过程中加入高盐清洗步骤 ,可有效降低宿主dNA残留量水平。对样品中自由巯基含量进行了测定 ,认为非还原电泳图谱中低分子量条带是由于抗体分子内存在自由巯基引起。

关键词：抗甲肝病毒单克隆抗体基因工程分离纯化鉴定亲和层析 rProtein A残留量

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大尺度生态水文模型的构建及其与GIS集成

生态学报 2003年第10期23卷 2115-2124页

作者：孙鹏森刘世荣中国林业科学研究院森林生态环境与保护研究所北京100091

基于过程模拟手段揭示森林植被的生态水文功能和变化机制 ,已经成为生态水文学研究的重要手段。由于陆地生态水文过程的非线性和尺度问题的广泛性 ,基于过程的坡面或小流域尺度的分布式水文模型不适合大流域的水文过程的分析和预测 ;另... 详细信息

基于过程模拟手段揭示森林植被的生态水文功能和变化机制 ,已经成为生态水文学研究的重要手段。由于陆地生态水文过程的非线性和尺度问题的广泛性 ,基于过程的坡面或小流域尺度的分布式水文模型不适合大流域的水文过程的分析和预测 ;另外 ,传统的水文模型主要侧重水文物理过程 ,只有充分耦合植被生态过程 ,才能从机制上揭示森林植被参与水文循环的调控作用。依据大流域的水文过程特点 ,从 5个方面阐述了大尺度生态水文模型构建过程中的主要问题 :1大尺度生态水文模型的概念和结构 ;2尺度的界定与匹配 ;3离散化数据集的建立 ,着重分析了植被覆盖、土壤质地、山地气候等主要数据集的建立方法 ;4分布式与集总式模型 ,这两类模型可以从他们的基本空间单元上进行区分 ,数字流域的建立和空间分析手段使得集总式模型和分布式模型得到了很好的结合 ;5生态水文模型与 GIS的集成 ,分析了 4种不同的集成方式 ,“松散型”的集成方式因其编程工作量小而被广泛采用。集成的目的不仅是要提高模拟的技术水平。

关键词：生态水文过程大尺度分布式模型地理信息系统离散化数据集

高发病率小儿人群中哮喘转归的改善:一项基于急诊科的随机临床试验结果

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世界核心医学期刊文摘（儿科学分册） 2006年第A10期2卷 24-25页

作者： Teach S.J. Crain E.F. Quint d.M. 俞晓梅 division of Emergency Medicine Children' s National Medical Center 111 Michigan Ave NWWashingtonDC 20010 United StatesDr.

Objective: To determine if an emergency department- based asthma follow- up clinic could improve outcomes within a high- morbidity pediatric population. design: Prospective, randomized clinical trial with 6 months of follow- up. Setting: Emergency department of an urban pediatric medical center. Participants: Convenience sample of 488 patients aged 12 months to 17 years, inclusive, with prior physician- diagnos- ed asthma and 1 or more other unscheduled visits in the previous 6 months and/or 1 or more hospitalizations in the prior 12 months. Intervention: Single follow- up clinic visit focusing on 3 domains: asthma self- monitoring and management, environmental modification and trigger control, and linkages and referrals to ongoing care. Main Outcome Measures: The primary outcome measurewas unscheduled visits for acute asthma care. Secondary outcomes included compliance with a medical plan and asthma quality of life. Analysis was by intention to treat with adjustment for baseline differences. Results: Of those randomized to the clinic visit,172 (70.5% ) of 244 attended. The intervention group had significantly fewer mean unscheduled visits for asthma care during follow- up (1.39 vs 2.34; relative risk [RR]=0.60[95% confidence interval (CI), 0.46- 0.77]). At 6 months, significantly more patients in the intervention group reported use of inhaled corticosteroids in the prior 2 days (49.3% vs 26.5% ;RR=2.03 [95% CI, 1.57- 2.62]), no limitation in daytime quality of life (43.8% vs 34.4% ; RR=1.36 [95% CI, 1.06- 1.73]), and no functional limitations in quality of life (49.8% vs 40.8% ; RR=1.33 [95% CI, 1.08- 1.63]). Conclusion: Attendance in the follow- up clinic was high. The intervention decreased subsequent unscheduled health care use while improving compliance and quality of life.

关键词：随机临床试验急诊科触发因素就诊情况糖皮质激素依从性生活质量就诊次数儿童医学中心自我

阿仑膦酸钠可改善原发性胆汁性肝硬化患者的骨密度:一项随机、安慰剂对照研究

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世界核心医学期刊文摘（胃肠病学分册） 2006年第4期2卷 44-45页

作者： Zein C.O Jorgensen R.A Clarke B. K.d. Lindor 陈瑜 division of Gastroenterology and Hepatology Mayo Clinic and Foundation 200 1st Street SW Rochester MN 55905 United StatesDr.

Bone loss is a well-recognized complication of primary biliary cirrhosis (PBC). Although it has been suggested that alendronate might improve bone mineral density (BMd) in PBC, no randomized placebo-controlled trial has been conducted. The primary aim of this study was to compare the effects of alendronate versus p lacebo on BMd and biochemical measurements of bone turnover in patients with PBC -associated bone loss. We conducted a double- blinded, randomized, placebo-controlled trial. Patients with a PBC and BMd t score of less than -1.5 were randomized to receive 70 mg per week of alendronat e or placebo over 1 year. BMd of the lumbar spine and proximal femur were measur ed at entry and at 1 year. Changes from baseline in BMd and biochemical measurem ents of bone turnover were assessed. Thirty-four patients were enrolled. Sevent een patients were randomized to each arm. After 1 year, a significantly larger i mprovement (P = .005) in spine BMd was observed in the alendronate group (0.09 ±0.03 g/cm2 Sd from baseline) comparedwith the placebo group (-0.003±0.02 g/c m2 Sd from baseline). A larger improvement (P = .046)was also observed in the fe moral BMd of alendronate patients versus placebo. BMd changes were independent o f concomitant estrogen therapy. The rate of adverse effects was similar in both groups. In conclusion, in patients with PBC-related bone loss, alendronate sign ificantly improves BMd compared with placebo. Although in this study oral alendr onate appears to be well tolerated in patients with PBC, larger studies are need ed to formally evaluate safety.

关键词：阿仑膦酸钠骨密度安慰剂对照骨丢失雌激素治疗股骨近端副作用发生率随机研究双盲研究持续

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二次肿瘤细胞减灭术对晚期卵巢癌的疗效

世界核心医学期刊文摘（妇产科学分册） 2005年第5期1卷 5-6页

作者： Rose P. G Nerenstone S Brady M. F. d. Mackey 宁亮 Gynecol. Oncol. Grp. Ad min. Office 4 Penn Center 1600 JFK Blvd. Philadelphia PA 19103 United States

BACKGROUNd: We evaluated the effect of adding secondary cytoreductive surgery to postoperative chemotherapy on progression-free survival and overall survival among patients who had advanced ovarian cancer and residual tumor exceeding 1 c m in diameter after primary surgery. METHOdS: Women were enrolled within six wee ks after primary surgery. If, after three cycles of postoperative paclitaxel plu s cisplatin, a patient had no evidence of progressive disease, she was randomly assigned to undergo secondary cytoreductive surgery followed by three more cycle s of chemotherapy or three more cycles of chemotherapy alone. RESULTS: We enroll ed 550 women. After completing three cycles of postoperative chemotherapy, 216 e ligible patients were randomly assigned to receive secondary surgical cytoreduct ion followed by chemotherapy and 208 to receive chemotherapy alone. Surgerywas d eclined by or medically contraindicated in 15 patients who were assigned to seco ndary surgery (7 percent). As of March 2003, 296 patients had died and 82 had pr ogressive disease. The likelihood of progression-free survival in the group ass igned to secondary surgery plus chemotherapy, as compared with the chemotherapy -alone group, was 1.07 (95 percent confidence interval, 0.87 to 1.31; P=0.54), and the relative risk of death was 0.99 (95 percent confidence interval, 0.79 to 1.24; P=0.92). CONCLUSIONS: For patients with advanced ovarian carcinoma in who m primary cytoreductive surgery was considered to be maximal, the addition of se condary cytoreductive surgery to postoperative chemotherapy with paclitaxel plus cisplatin does not improve progression-free survival or overall survival.

关键词：晚期卵巢癌肿瘤细胞减灭术存活时间顺铂化疗残存肿瘤单纯化疗组肿瘤进展似然比

高分辨率超声检查用于检测中心性浆液性脉络膜视网膜病变

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世界核心医学期刊文摘（眼科学分册） 2005年第8期1卷 31-32页

作者： doro d. Visentin S. Maimone P.E. Pilotto E. 潘佳鸿 dept. of Neurosci. -Ophthalmology Via Giustiniani 2 Padova 35128 Italy Dr.

PURPOSE: To investigate the use of high-resolution ultrasonography for detect ing choroidal layer abnormalities in eyes with central serous chorioretinopathy (CSC). dESIGN: Prospective observational case series. METHOd: Optical coherence tomography (OCT) and high-resolution ultrasonography with a 20-MHz immersion p robe were performed bilaterally in 10 patients, of whom 5 were affected by unila teral active CSC and 5 by unilateral cystoid macular edema (CME). Ten age-match ed control subjects were also investigated. RESULTS: Both OCT and high-resoluti on ultrasonography showed a macular elevation in eyes with CSC and CME. High-re solution ultrasonography has shown evidence of a nonechogenic linear band under the retinal pigment epithelium in patients affected by CSC. This could be found in neither patients with CME or in control subjects. CONCLUSION: Highresolution ultrasonography can detect a nonechogenic space consistent with hyperpermeabilit y of choroidal capillaries in eyes with active CSC. Shadowing by an altered reti nal pigment epithelium cannot be ruled out, however.

关键词：高分辨率超声视网膜色素上皮色素上皮层脉络膜毛细血管黄斑囊样水肿黄斑部水浸探头异常病变声影通透性

1例色素性视网膜炎患者行包括视网膜色素上皮层的胎儿视网膜移植后的视力变化

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世界核心医学期刊文摘（眼科学分册） 2005年第1期1卷 30-31页

作者：秦雪娇 Radtke N.d Aramant R.B Seiler M.J 3Audubon Plaza dr LouisvilleKY 40217United States.Dr

Objective:To report the subjective and objective im-provement in vision in a patient with autosomal dominant retinitis pigmentosa after transpl antation of a sheet of fe-tal neural retina together with its r etinal pigment ***:A sheet of fetal n eural retina with its retinal pigment epithelium was tran splanted into the subretinal space under the fovea uni laterally in a patient with retinitis pigmentosa with visu al acuity of 20/800in the treated *** Treatment dia betic Retinopathy Study visual acuity testing,scanni ng laser ophthalmo-scope,tissue typing of the donor and recipient,fluo-rescein angiography,multifocal electroretinogram,multi-focal visually evoked potential,an d clinical examination were ***:No clinical evid ence of rejection was *** was no retinal ed ema or *** transplant sheet lost its pigmen tation by *** Outcome Measures:A change in visual acuity from20/800to 20/400(7months),20/250(9months),and 20/160(1year)was observed by Early Treatment diabetic Retinopathy Study visual a cuity ***-pendently,scanning laser ophthalm oscope testing at a different institution at 9months sh owed a visual acuity of20/270at a 40°field of ***:This study indicates that fetal retina transpl anted with its retinal pigment epithelium can survive 1year without apparent clinical evidence of rejection and s how continued im-provement in Early Treatment diabetic Retinopathy Study visual acuity.

关键词：视网膜色素上皮色素性视网膜炎视力变化网膜移植视网膜电图神经视网膜视网膜水肿激光扫描检眼镜黄斑区视网膜

End-tidal capnometry during emergency department procedural sedation and analgesia： a randomized, controlled study

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World Journal of Emergency Medicine 2016年第1期7卷 13-18页

作者： Samuel G. Campbell Kirk d. Magee Peter J. Zed Patrick Froese Glenn Etsell Alan LaPierre donna Warren Robert R. MacKinley Michael B. Butler George Kovacs david A. Petrie department of Emergency Medicine and Anesthesia Dalhousie University Halifax Nova Scotia Canada Faculty of Pharmaceutical Sciences and department of Emergency Medicine University of British Columbia Vancouver BC Canada Charles V. Keating Emergency and Trauma Centre Halifax Nova Scotia Canada department of Emergency Medicine Anesthesia and Anatomy Dalhousie University Halifax Nova Scotia Canada

BACKGROUNd:This prospective,randomized trial was undertaken to evaluate the utility of adding end-tidal capnometry(ETC)to pulse oximetry(PO)in patients undergoing procedural sedation and analgesia(PSA)in the emergency department(ed).METHOdS:The patients were randomized to monitoring with or without ETC in addition to the current standard of *** endpoints included respiratory adverse events,with secondary endpoints of level of sedation,hypotension,other PSA-related adverse events and patient ***:Of 986 patients,501 were randomized to usual care and 485 to additional ETC *** this series,48%of the patients were female,with a mean age of 46 *** manipulations(71%),cardioversion(12%)and abscess incision and drainage(12%)were the most common procedures,and propofol and fentanyl were the sedative/analgesic combination used for most *** was no difference in patients experiencing de-saturation(Sa O2ed in 16(3.3%)patients in the ETC group and 7(1.4%)in the control group(P=0.048).CONCLUSIONS:The addition of ETC does not appear to change any clinically significant *** found an increased incidence of the use of airway repositioning maneuvers and hypotension in cases where ETC was *** do not believe that ETC should be recommended as a standard of care for the monitoring of patients undergoing PSA.

关键词： Procedural sedation and analgesia Capnography Adverse events Emergency medicine

6-巯基嘌呤治疗炎症性肠病的单中心10年治疗经验

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世界核心医学期刊文摘（胃肠病学分册） 2005年第7期1卷 40-41页

作者： Glazier K.d. Palance A.L. Griffel L.H. das K.M. 樊菁 UMdNJ- Robert Wood Johnson Med. Sch. 1 Robert Wood Johnson Place New Brunswick NJ 08903 United States Dr.

Goals: To report the 10- year experience of a single center in treating pati ents with refractory inflammatory bowel disease (IBd) with relatively lower dose of 6- mercaptopurine (6- MP). Study: The charts of 285 patients with IBd (Cro hn’ s disease 160 and ulcerative colitis 125) receiving 6- MP were reviewed. C linical response, subsequent breakthrough while taking 6- MP, and relapse rates when 6- MP was discontinued and side effects were assessed. Results: Ninety- three percent of the patients were taking 50 to 75 mg/day of 6- MP. Complete re mission was achieved in 62% , partial remission in 14.5% , and failure to achi eve remission in 23.5% of the patients. Of complete responders, 27.5% had br eakthrough while continuing 6- MP. Nine percent of those that achieved a comple te remission experienced a relapse after 6- MP was discontinued. Side effects i ncluded leukopenia (11.2% ), abnormal liver function tests (3.8% ), various in fections, including pneumonia (3.1% ), pancreatitis (2.5% ), nausea (2.1% ), headache (2.8% ), fever (1.4% ), hair loss (1% ), and rash (0.7% ). Two canc ers occurred while taking 6- MP: melanoma on the finger and a fatal colonic lym phoma. Four patients continued 6- MP throughout pregnancies and had normal outc omes. Conclusions: In our experience 6- MP is relatively safe and appears to be as effective at a lower dosage (0.84 mg/kg per day) compared with the recommend ed higher dosage (1- 1.5 mg/kg per day), when leukopenia was more frequent. Ser ious side effects, although rare, need to be monitored.

关键词：炎症性肠病单中心克罗恩病溃疡性结肠炎结肠淋巴瘤临床反应复发率黑色素瘤白细胞减少正常分娩