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Association between the Phenotypes and Genotypes of Antimicrobial Resistance in Haemophilus parasuis Isolates from Swine in quang Binh and Thua Thien Hue Provinces, Vietnam

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Engineering 2020年第1期6卷 40-48页

作者： Chao Nguyen Van Lijun Zhang Tam Vu Thi Thanh Hung Pham Hoang Son tuan Tran Ngoc Qi Huang Rui Zhou State Key Laboratory of Agricultural Microbiology College of Veterinary MedicineHuazhong Agricultural UniversityWuhan 430070China Faculty of Animal Science and Veterinary Medicine University of Agricultural and ForestryHue UniversityHue 53000Vietnam Mientrung Institute for Scientific Research Vietnam Academy of Science and TechnologyHue 53000Vietnam Guangdong Provincial Key Laboratory of Marine Biology Shantou UniversityShantou 515063China Cooperative Innovation Center of Sustainable Pig Production Wuhan 430070China International Research Center for Animal Diseases The Ministry of Science and TechnologyWuhan 430070China

Haemophilus parasuis (H. parasuis) is one of the bacterial pathogens of great concern as it causes huge economic losses to the swine industry worldwide. One of the reasons why the control of H. parasuis has failed is the increase in antimicrobial resistance (AMR). The country of Vietnam has the second-largest pig production in Asia. However, there is still a lack of data about the AMR prevalence of H. parasuis in *** purpose of this study is to investigate the prevalence of AMR and analyze the association between AMR and AMR genes (ARGs). The H. parasuis strains used in this research were isolated from swine in the quang Binh and Thua Thien Hue Provinces, Central Vietnam, as reported in our previous study. All of the strains were tested for AMR against 25 antibacterial agents using the broth microdilution method and for the presence of ARGs using the polymerase chain reaction (PCR) method. The tested strains were shown to have a high frequency of resistance to trimethoprim/sulfamethoxazole (94.6%), followed by resistance to colistin, chloramphenicol, gentamicin, penicillin, lincomycin, and amoxicillin. The most prevalent ARGs in these strains were blaTEM-1 (94.6%), int (76.8%), gyrA (58.9%), and rmtD (50.0%). Cefuroxime, chloramphenicol, and tobramycin resistances were strongly correlated with the presence of the ARGs blaROB-1 (odds ratio (OR) = 26.3, 95% confidence interval (CI) 2.7–255.7, p = 0.002), catl (OR = 25.1, 95% CI 2.4–258.9, p = 0.004), and strB (OR = 23.5, 95% CI 2.6–212.6, p = 0.001), *** study reveals for the first time the current situation of H. parasuis AMR in Central Vietnam, which is helpful for the clinical control of this disease, as well as for the development of policies and clinical practice guidelines to reduce AMR in swine production in Central Vietnam.

关键词： Haemophilus parasuis Antimicrobial resistance Antimicrobial resistance gene Vietnam

The Effect of ENSO on Hydrological Structure and Environment in the South Central Coast-Vietnam

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Journal of Marine Science 2020年第1期2卷 10-16页

作者： Pham Xuan duong Hoang Trung Du Vo Tran tuan Linh To Duy Thai Phan Minh Thu Institute of Oceanography Vietnam Academy of Science and Technology(VAST)Nha TrangVietnam State Key Laboratory of Estuarine and Coastal Research(SKLEC) East China Normal UniversityShanghaiChina Graduate University of Science and Technology VASTHa NoiVietnam

ENSO(El Niño-Southern Oscillation)phenomena have impacted on the hydrodynamic regime and environmental factors of the tropical ocean in *** case of Vietnamese South-Central Waters,impacts of ENSO only focused on issues of changing seasonal wind,seawater temperature anomalies,changing of water masses as the air-sea *** on several data sets collecting in the period of 2003-2017,new finding of seawater temperature,salinity and environmental factors was identified in the water masses of Vietnamese South-Central *** highest salinity was 35.4‰.During the El Nino event,increasing water temperature and salinity caused to move the deeper water masses to be closer to the sea surface than that initial depth in the neutral *** the La Nina event,the temperature of most water masses reduced by 0.1-3.0℃,and then these water masses could be affected to the deeper *** the phase from strong ENSO event towards the neutral time,nutrient salts of the 4 water masses were lower concentration in the neutral year,causing the lack of phosphorus in sea surface water masses.

关键词： Effect of ENSO Hydrodynamic regime South Central Coast-Vietnam

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越南Orgarnicare展示商铺

现代装饰 2019年第6期 36-41页

作者：无 Nguyēn Hai Long Tran Thi Ngu Ngon Nguyēn tuan Dang Pham Khánh Duy LêVan Duy Bao Saurav Shah quang Dam(摄影) 范嘉苑(编译) Tropical Space 越南Tropical Space

Orgarnicare项目是一个为越南传统鱼露和有机产品而打造的展示空间。该项目由一座建于1975年之前的旧房子改造而成,其本身已历经过数次加固及扩建。对于此次的展示商铺改造,设计师旨在用具有越南特色的传统建材构建空间,让独具热带风情... 详细信息

Orgarnicare项目是一个为越南传统鱼露和有机产品而打造的展示空间。该项目由一座建于1975年之前的旧房子改造而成,其本身已历经过数次加固及扩建。对于此次的展示商铺改造,设计师旨在用具有越南特色的传统建材构建空间,让独具热带风情的空间元素与传统越南鱼露相互映衬,产生有趣的对话。

关键词：框架系统 Orgarnicare 热带风情

Assessment of Hydrological Drought Using the Standardized Streamflow Index (SSFI): A Case Study of the Tien Yen River Basin of quang Ninh Province, Vietnam

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Journal of Geoscience and Environment Protection 2022年第8期10卷 309-326页

作者： Nguyen Van Hieu Nguyen Van tuan Nguyen Khac Bang Pham Hoang Hai Le Vinh Ha Tran Thi Hoa Institute of Water Resources Planning Ha Noi Vietnam Institute of Environment and Public Health VUSTA Ha Noi Vietnam Thai Binh University Thai Binh Province Thai Binh Vietnam

Probabilistic assessment of drought plays an important role in providing valuable information for evaluating water resources systems under drought conditions, and bivariate copulas are effective and efficient for the probabilistic assessment of drought based on joint distributions and/or joint return periods of drought characteristics. In this study, hydrological drought events and their characteristics (including duration and severity) in the Tien Yen River Basin of quang Ninh province are detected using the Standardized Streamflow Index (SSFI). The BB8Copula is selected as the best-fit copula for hydrological drought duration and severity. Joint probabilities and joint return periods of drought duration and severity in the cases “and” and “or” are calculated based on the BB8Copula, which are employed for drought assessment. The results show that the drought events with 1-season or cross-quarter duration were more popular than others;joint probabilities and joint return periods of the detected drought events from 1962 to 2009, ranged from 0.2% to 92.2% and from 0.782 years to 315.414 years, respectively, in the case “and”, and ranged from 3.8% to 99.6% and from 0.724 years to 18.785 years, respectively, in the case “or”.

关键词： Probabilistic Assessment Standardized Streamflow Index (SSFI) Hydrological Drought Drought Characteristics Copula

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越南松树枯死病症状及原因初步研究

湖南林业科技 2016年第1期43卷 6-11页

作者： Nguyen Thanh tuan Le Bao Thanh 颜学武南京林业大学森林资源与环境学院江苏南京210037 越南林业大学森林资源管理与环境学院越南河内100803 湖南省林业科学院湖南长沙410004

为查明越南松树枯死的原因,对越南南部的林同省,中部的承天-顺化省,北部的广宁省的思茅松、马尾松和南亚松枯死症状和原因进行了调查,分别从枯死、濒死、健康松树木质部中抽取52份样品,用于线虫分离镜检。调查发现松树在感病后,树冠部... 详细信息

为查明越南松树枯死的原因,对越南南部的林同省,中部的承天-顺化省,北部的广宁省的思茅松、马尾松和南亚松枯死症状和原因进行了调查,分别从枯死、濒死、健康松树木质部中抽取52份样品,用于线虫分离镜检。调查发现松树在感病后,树冠部分针叶失去光泽,逐渐褪绿变黄,进而褐红干枯,最后整个树冠变为褐色或红褐色,全株枯死,针叶下垂且当年不落;随着病程的发展,松脂分泌逐渐减少,直至停止;树干上有天牛产卵刻槽,死亡的松树上有天牛的羽化孔。在抽取的松树样品中分离鉴定到了泰国伞滑刃线虫(Bursaphelenchus thailandae Brassch&Brassch-Bidasak,2002),为病原的确定和防治工作提供初步的依据。

关键词：越南松树天牛线虫

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越南松树中泰国伞滑刃线虫的分离与鉴定

华中昆虫研究 2017年第1期 117-122页

作者： Nguyen Thanh tuan Le Bao Thanh 颜学武越南林业大学湖南省林业科学院

2012-2013年从越南3个省调查采集到病死松树样本52份。将病死松木中所获线虫分离、提纯、培养后,通过显微测量及Deman公式计算所获线虫的V、a、b等相关部位数值,并对其形态特征进行描述。初步结果表明在样本中仅分离到1种伞滑刃属线虫,... 详细信息

2012-2013年从越南3个省调查采集到病死松树样本52份。将病死松木中所获线虫分离、提纯、培养后,通过显微测量及Deman公式计算所获线虫的V、a、b等相关部位数值,并对其形态特征进行描述。初步结果表明在样本中仅分离到1种伞滑刃属线虫,即泰国伞滑刃线虫(Bursaphelenchus thailandae Brassch&Brassch-Bidasak,2002)。通过对其ITS序列的研究,以伞滑刃属线虫ITS区通用引物P1:5’-CGTAACAAGGTAGCTGTAG-3’,P2:5’-TTTCACTCGCCGTTACTAAGG-3’,分别对分离得到的线虫进行PCR扩增,得到880bp左右的扩增片段。通过克隆并测序,然后进行测序分析及比对,结果鉴定出线虫种群为泰国伞滑刃线虫(***),这也与前部分形态鉴定相符合。这是越南关于松树上伞滑刃属线虫种类研究的首次报道。

关键词：越南伞滑刃属松树寄生线虫种类鉴定

特色紫甜玉米自交系选育高营养水果型杂交玉米的应用研究

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南方农业学报 2022年第7期53卷 1843-1854页

作者：范光俊阮忠德阮氏月英武文烈武氏春萍吕荣华越南农业大学越南河内100803 广西农业科学院国际合作处广西南宁530007

【目的】通过紫色糯玉米(花青素型)与白色甜玉米(超甜糯型)杂交,选育出营养丰富的水果型玉米,以满足越南乃至世界对高营养水果型玉米的市场需求,也为水果型玉米的杂交选育提供参考。【方法】将4个紫色糯玉米品系(♀)与白色甜玉米(♂)进... 详细信息

【目的】通过紫色糯玉米(花青素型)与白色甜玉米(超甜糯型)杂交,选育出营养丰富的水果型玉米,以满足越南乃至世界对高营养水果型玉米的市场需求,也为水果型玉米的杂交选育提供参考。【方法】将4个紫色糯玉米品系(♀)与白色甜玉米(♂)进行杂交后再回交,通过形态标记(籽粒皱缩且颜色为深紫色)从BC2F1代中共筛选出20个品系,再经5代连续自交获得80个自交系(UV01~UV80),从中选择20个优良自交系。利用Line×Tester模型将20个优良自交系与2个测试种[黄色甜玉米自交系SW1(TI)和白色甜玉米自交系D181(T2)]进行测配,获得40个杂交组合(TH01~TH40),通过随机区组设计在越南河内田间开展种植试验,以SW1011和Fancy111为对照品种。【结果】TH08、TH17、TH18、TH27、TH31和TH35等6个杂交组合的平均产量较高,为13.4~14.4 t/ha,与对照品种SW1011无显著差异(P>0.05),说明这些杂交组合具有较高的商业潜力;UV03、UV07、UV08、UV10、UV12、UV16、UV18、UV42、UV71等9个品系的锤度>16.0%、果皮薄100.0 mg/100 g,属于超甜紫甜玉米品系。UV10、UV24、UV40、UV46和UV71等5个品系在商品产量方面具有较高的一般配合力(GCA),UV12和UV16品系在甜度指数方面具有较高的GCA,UV35、UV36、UV38、UV46和UV73等5个品系在花青素含量方面具有较高的GCA。【结论】UV10和UV12在产量和品质方面均具有较高的GCA,为2个营养丰富的水果型超甜紫甜玉米品系,可在越南及其他适用地区推广种植。

关键词：紫甜玉米自交系 Smith-Hazel指数一般配合力(GCA) Line×Tester模型

越南枯死松树症状特征和体内寄生线虫种类调查

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南京林业大学学报（自然科学版） 2016年第1期40卷 44-52页

作者：阮成俊谈家金叶建仁林司曦南京林业大学林学院南京林业大学南方现代林业协同创新中心江苏南京210037 Faculty of Forest Resources and Envionmental Management Vietnam Forestry UniversityHanoi 156204Vietnam

为确定造成越南松林的枯死是否为松材线虫所为,对越南南部的林同省、中部的承天-顺化省、北部的广宁省的思茅松(Pinus kesiyaRoyle)、马尾松(***.)和南亚松(*** et de Vries)松树枯死现象及症状特点进行了调查,分别从52株枯死、濒死、... 详细信息

为确定造成越南松林的枯死是否为松材线虫所为,对越南南部的林同省、中部的承天-顺化省、北部的广宁省的思茅松(Pinus kesiyaRoyle)、马尾松(***.)和南亚松(*** et de Vries)松树枯死现象及症状特点进行了调查,分别从52株枯死、濒死、健康松树木质部中抽取木质部样品,进行线虫分离,根据形态学特点确定这些松树体内寄生线虫的种类。调查发现松树在枯死过程中,树冠部分针叶失去光泽,逐渐褪绿变黄,进而褐红干枯,最后整个树冠变为褐色或红褐色,全株枯死,针叶下垂且当年不落;随着病程的发展,松脂分泌逐渐减少,直至停止;树干上有天牛产卵刻槽,死亡的松树上有天牛的羽化孔。在抽取的松树样品中分离鉴定到了泰国伞滑刃线虫(Bursaphelenchus thailandae)、悬臂伞滑刃线虫(***)、中华伞滑刃线虫(***)、树木伞滑刃线虫(***)、托斯卡纳伞滑刃线虫(***)、莱奴尔夫伞滑刃线虫(***)、云杉小蠹伞滑刃线虫(***)和伞滑刃属线虫未定种(***.),调查中未发现重要检疫性病原松材线虫(***)。

关键词：松树枯死症状寄生线虫越南

紫甜玉米产量及其构成因素和品质性状的杂种优势和配合力分析

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南方农业学报 2022年第5期53卷 1193-1206页

作者：范光俊阮忠德阮氏月英武氏春萍武文烈吕荣华邹成林吕佳茗越南农业大学越南河内100803 广西农业科学院国际合作处广西南宁530007 广西农业科学院玉米研究所广西南宁530007 广西大学计算机与电子信息学院广西南宁530004

【目的】对20个紫甜玉米自交系与2个测试种进行产量及其构成因素和品质性状的杂种优势和配合力分析,为高效创制高产甜玉米自交系提供材料基础和理论依据。【方法】将20个紫甜玉米自交系(TD01~TD20)与2个测试种[黄色甜玉米自交系(TI)和... 详细信息

【目的】对20个紫甜玉米自交系与2个测试种进行产量及其构成因素和品质性状的杂种优势和配合力分析,为高效创制高产甜玉米自交系提供材料基础和理论依据。【方法】将20个紫甜玉米自交系(TD01~TD20)与2个测试种[黄色甜玉米自交系(TI)和白色甜玉米自交系(T2)]进行杂交组配,采用完全随机组设计对获得的40个杂交组合(TH01~TH40)进行田间试验,以SW1011和Fancy 111为对照品种。测定亲本及其杂交组合的产量及其构成因素和品质性状。利用Line×Tester模型在AGD-R软件中进行中亲优势度(Hm)、杂种优势度(Hb)和一般配合力(GCA)分析。【结果】紫色种皮由父本遗传,在40个杂交组合中占优势,所有测定的性状表现为中亲优势和杂种优势。大多数亲本系鲜穗收获期的Hm、Hb和GCA表现为负值,说明这些亲本系具有组配早熟杂交种的潜力。除收获期和穗粗外,其余调查性状表现出多个显性效应,证明了非加性遗传效应的重要性。具有较高GCA的杂交组合为TH05(T1×TD05)、TH31(T2×TD11)、TH20(T1×TD20)、TH33(T2×TD13)、TH34(T2×TD14)、TH16(T1×TD16)、TH08(T1×TD08)和TH25(T2×TD05)。【结论】紫色甜玉米与黄色甜玉米自交系或白色甜玉米自交系杂交,紫色性状具有显性效应,筛选出的8个品质优良杂交种及其亲本可作为培育越南商品化紫甜玉米品种的亲本材料。

关键词：紫甜玉米产量品质配合力杂交优势