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The role of DNA methylation in the maintenance of phenotypic variation induced by grafting chimerism in Brassica

Horticulture Research 2023年第3期10卷 164-175页

作者： Ke Liu Tingjin Wang duohong xiao Bin Liu Yang Yang Kexin Xu Zhenyu Qi Yan Wang Junxing Li Xun Xiang Lu Yuan Liping Chen Department of Horticulture College of Agriculture and BiotechnologyZhejiang UniversityHangzhou 310058China Agricultural Experiment Station Zhejiang UniversityHangzhou 310058China

Grafting facilitates the interaction between heterologous cells with different genomes,resulting in abundant phenotypic variation,which provides opportunities for crop improvement.However,how grafting-induced variation occurs and is transmitted to progeny remains elusive.A graft chimera,especially a periclinal chimera,which has genetically distinct cell layers throughout the plant,is an excellent model to probe the molecular mechanisms of grafting-induced variation maintenance.Here we regenerated a plant from the T-cell layer of a periclinal chimera,TCC(where the apical meristem was artificially divided into three cell layers–from outside to inside,L1,L2,and L3;T=Tuber mustard,C=red Cabbage),named rTTT0(r=regenerated).Compared with the control(rsTTT,s=self-grafted),rTTT0 had multiple phenotypic variations,especially leaf shape variation,which could be maintained in sexual progeny.Transcriptomes were analyzed and 58 phenotypic variation-associated genes were identified.Whole-genome bisulfite sequencing analyses revealed that the methylome of rTTT0 was changed,and the CG methylation level was significantly increased by 8.74%.In rTTT0,the coding gene bodies are hypermethylated in the CG context,while their promoter regions are hypomethylated in the non-CG context.DNA methylation changes in the leaf shape variation-associated coding genes,ARF10,IAA20,ROF1,and TPR2,were maintained for five generations of rTTT0.Interestingly,grafting chimerism also affected transcription of the microRNA gene(MIR),among which the DNA methylation levels of the promoters of three MIRs associated with leaf shape variation were changed in rTTT0,and the DNA methylation modification of MIR319 was maintained to the fifth generation of selfed progeny of rTTT0(rTTT5).These findings demonstrate that DNA methylation of coding and non-coding genes plays an important role in heterologous cell interaction-induced variation formation and its transgenerational inheritance.

关键词： phenotypic maintained grafting

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Bioinspired polysaccharide-based nanocomposite membranes with robust wet mechanical properties for guided bone regeneration

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National Science Review 2024年第3期11卷 215-226页

作者： Jian-Hong xiao Zhen-Bang Zhang JiaHao Li Si-Ming Chen Huai-Ling Gao YinXiu Liao Lu Chen ZiShuo Wang YiFan Lu YuanZhen Hou HengAn Wu duohong Zou Shu-Hong Yu Department of Chemistry New Cornerstone Science Laboratory Institute of Biomimetic Materials&Chemistry Anhui Engineering Laboratory of Biomimetic MaterialsDivision of Nanomaterials&Chemistry Hefei National Research Center for Physical Sciences at the Microscale University of Science and Technology of China Department of Dental Implant Center Stomatologic Hospital and College Key Laboratory of Oral Diseases Research of Anhui Province Anhui Medical University CAS Key Laboratory of Mechanical Behavior and Design of Materials Department of Modern Mechanics CAS Center for Excellence in Complex System Mechanics University of Science and Technology of China Department of Oral Surgery College of Stomatology National Clinical Research Center for Oral DiseasesShanghai Key Laboratory of Stomatology Shanghai Research Institute of Stomatology Shanghai Ninth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine Institute of Innovative Materials(I2M) Department of Chemistry Department of Materials Science and Engineering Southern University of Science and Technology

Polysaccharide-based membranes with excellent mechanical properties are highly desired. However, severe mechanical deterioration under wet conditions limits their biomedical applications. Here, inspired by the structural heterogeneity of strong yet hydrated biological materials, we propose a strategy based on heterogeneous crosslink-and-hydration（HCH） of a molecule/nano dual-scale network to fabricate polysaccharide-based nanocomposites with robust wet mechanical properties. The heterogeneity lies in that the crosslink-and-hydration occurs in the molecule-network while the stress-bearing nanofiber-network remains unaffected. As one demonstration, a membrane assembled by bacterial cellulose nanofiber-network and Ca2+-crosslinked and hydrated sodium alginate molecule-network is designed. Studies show that the crosslinked-and-hydrated molecule-network restricts water invasion and boosts stress transfer of the nanofiber-network by serving as interfibrous bridge. Overall, the molecule-network makes the membrane hydrated and flexible; the nanofiber-network as stress-bearing component provides strength and toughness.The HCH dual-scale network featuring a cooperative effect stimulates the design of advanced biomaterials applied under wet conditions such as guided bone regeneration membranes.

关键词： bioinspiration heterogeneous crosslink-and-hydration dual-scale network nanocomposite membrane wet mechanical properties guided bone regeneration

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A meteorologically adjusted ensemble Kalman filter approach for inversing daily emissions:A case study in the Pearl River Delta,China

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Journal of Environmental Sciences 2022年第4期34卷 233-248页

作者： Guanglin Jia Zhijiong Huang xiao Tang Jiamin Ou Menghua Lu Yuanqian Xu Zhuangmin Zhong Qing’e Sha Huangjian Wu Chuanzeng Zheng Tao Deng duohong Chen Min He Junyu Zheng School of Environment and Energy South China University of TechnologyUniversity Town CampusGuangzhou 510006China Institute for Environmental and Climate Research Jinan UniversityGuangzhou 511486China Institute of Atmospheric Physics Chinese Academy of SciencesBeijing 100029China Faculty of Social and Behavioural Sciences Utrecht UniversityUtrechtCH 3584the Netherlands Guangzhou Institute of Tropical and Marine Meteorology China Meteorological AdministrationGuangzhou 510640China State Environmental Protection Key Laboratory of Regional Air Quality Monitoring Guangdong Environmental Monitoring CenterGuangzhou 510308China Department of Environmental Science and Engineering College of Architecture and EnvironmentSichuan UniversityChengdu 610065China

The conventional Ensemble Kalman filter(EnKF),which is now widely used to calibrate emission inventories and to improve air quality simulations,is susceptible to simulation errors of meteorological inputs,making accurate updates of high temporal-resolution emission inventories challenging.In this study,we developed a novel meteorologically adjusted inversion method(MAEInv)based on the EnKF to improve daily emission estimations.The new method combines sensitivity analysis and bias correction to alleviate the inversion biases caused by errors of meteorological inputs.For demonstration,we used the MAEInv to inverse daily carbon monoxide(CO)emissions in the Pearl River Delta(PRD)region,China.In the case study,60%of the total CO simulation biases were associated with sensitive meteorological inputs,which would lead to the overestimation of daily variations of posterior emissions.Using the new inversion method,daily variations of emissions shrank dramatically,with the percentage change decreased by 30%.Also,the total amount of posterior CO emissions estimated by the MAEInv decreased by 14%,indicating that posterior CO emissions might be overestimated using the conventional EnKF.Model evaluations using independent observations revealed that daily CO emissions estimated by MAEInv better reproduce the magnitude and temporal patterns of ambient CO concentration,with a higher correlation coefficient(R,+37.0%)and lower normalized mean bias(NMB,-17.9%).Since errors of meteorological inputs are major sources of simulation biases for both low-reactive and reactive pollutants,the MAEInv is also applicable to improve the daily emission inversions of reactive pollutants.

关键词： Emission inversion Daily emissions Meteorological adjustment Ensemble Kalman filter

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壳聚糖-细菌纤维素复合膜的制备及其生物相容性的研究

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安徽医科大学学报 2022年第8期57卷 1177-1181页

作者：肖剑虹赵正宜邹多宏安徽医科大学口腔医学院安徽医科大学附属口腔医院安徽省口腔疾病研究重点实验室合肥230032 上海交通大学医学院附属第九人民医院·口腔医学院口腔外科上海市口腔医学重点实验室上海200001

目的通过制备壳聚糖(CS)-细菌纤维素(BC)复合膜,并测试该复合膜在干态和湿态下的拉伸强度及其生物相容性,探究CS-BC复合膜作为引导骨再生膜的可行性。方法将CS和BC按不同的质量比混合,超声破碎使两者分散均匀,通过自蒸发分别制备出纯CS... 详细信息

目的通过制备壳聚糖(CS)-细菌纤维素(BC)复合膜,并测试该复合膜在干态和湿态下的拉伸强度及其生物相容性,探究CS-BC复合膜作为引导骨再生膜的可行性。方法将CS和BC按不同的质量比混合,超声破碎使两者分散均匀,通过自蒸发分别制备出纯CS膜和CS∶BC分别为10∶1、10∶3、10∶5、10∶7和10∶9的复合膜,将制备的各组膜通过氢氧化钠乙醇溶液浸泡除酸处理后备用。采用力学万能实验机来测试各组膜分别在干态和湿态下的拉伸强度(n=6)。通过扫描电镜和透射电镜观察拉伸强度最大组复合膜的微观结构,而其成分构成则通过傅立叶变换红外光谱仪和X-射线衍射进行表征。将大鼠骨髓干细胞分别与空白对照组(不加膜)、纯CS膜组和拉伸强度最大的复合膜组(n=5)共培养1、4、7 d后通过CCK-8法检测各组细胞存活率。结果在CS基质中加入BC后,扫描电镜观察示复合膜的横截面呈现出有序的层状结构,傅立叶变换红外光谱分析及X-射线衍射分析显示复合膜中存在BC。CS-BC复合膜的拉伸强度随BC比例增加呈现出先增加后降低的趋势,在CS和BC的质量分数比为10∶7时该复合膜在干、湿态的拉伸强度均达到最大,分别为(204.7±63.0)、(44.4±6.4)MPa。与大鼠骨髓干细胞共培养1、4、7 d后,纯CS膜组、CS-BC复合膜组(n=5)大鼠骨髓干细胞的数量与空白组对比差异均无统计学意义。结论CS∶BC为10∶7时,复合膜在干态和湿态的拉伸强度最大,且具有良好的细胞相容性。

关键词：壳聚糖细菌纤维素复合膜拉伸强度生物相容性材料

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聚乙烯醇-细菌纤维素复合可吸收生物屏障膜的制备及理化性能研究

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安徽医科大学学报 2022年第10期57卷 1513-1517页

作者：赵正宜肖剑虹刘重远邹多宏安徽医科大学口腔医学院安徽医科大学附属口腔医院安徽省口腔疾病研究重点实验室合肥230032 上海交通大学医学院附属第九人民医院·口腔医学院口腔外科上海市口腔医学重点实验室上海200001

目的通过测试聚乙烯醇(PVA)-细菌纤维素(BC)复合可吸收生物屏障膜的拉伸强度,并检测其对鼠胚胎成纤维细胞增殖的影响,研究其作为新型骨组织工程生物屏障膜的可行性。方法通过自沉积的方法制备不同比例PVA-BC共混膜及纯PVA膜。采用拉伸... 详细信息

目的通过测试聚乙烯醇(PVA)-细菌纤维素(BC)复合可吸收生物屏障膜的拉伸强度,并检测其对鼠胚胎成纤维细胞增殖的影响,研究其作为新型骨组织工程生物屏障膜的可行性。方法通过自沉积的方法制备不同比例PVA-BC共混膜及纯PVA膜。采用拉伸实验测试不同比例组别样品抗拉强度,取抗拉强度最大复合膜组浸泡于去离子水中测量其湿态抗拉强度。取抗拉强度最大复合膜组及纯PVA膜组,用扫描电镜观察其结构。对PVA、BC和PVA-BC复合膜进行X射线衍射和傅里叶变换红外光谱分析。分别将两组薄膜与NIH/3T3共培养后用CCK-8法检测各组细胞存活率,观察其生物相容性。结果制备出均质PVA-BC复合膜,X射线衍射和傅立叶变换红外光谱分析显示复合膜中存在PVA和细菌纤维素,复合膜抗拉强度最初随BC比例增加而增加,当PVA和BC质量分数比为10∶7时抗拉强度最大,达(155.5±14.7)MPa,该组湿态下强度达(13.8±1.2)MPa。细胞培养第1、4、7天后PVA-BC复合膜、纯PVA膜及空白组之间无明显差异(P>0.05)。结论PVA-BC共混膜干湿态下均具备一定的机械性能及良好的生物相容性,具有一定的临床应用前景。

关键词：聚乙烯醇细菌纤维素共混液仿生结构材料

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珠三角关键大气挥发性有机物的模拟精度评估

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大气科学 2021年第5期45卷 1114-1126页

作者：张佩文唐晓陈科艺陈多宏朱莉莉沈劲叶斯琪孔磊韩丽娜吴倩王自发成都信息工程大学成都610225 中国科学院大气物理研究所大气边界层物理和大气化学国家重点实验室北京100029 广东省环境监测中心广东510308 中国环境监测总站北京100012

大气挥发性有机物(VOCs)是导致臭氧污染的关键前体物,是城市空气质量建模不可或缺的重要组成部分,但由于其非常复杂的构成和来源以及监测数据缺乏,目前对其模拟精度的了解仍非常有限。本文利用嵌套网格空气质量模式预报系统(NAQPMS)对... 详细信息

大气挥发性有机物(VOCs)是导致臭氧污染的关键前体物,是城市空气质量建模不可或缺的重要组成部分,但由于其非常复杂的构成和来源以及监测数据缺乏,目前对其模拟精度的了解仍非常有限。本文利用嵌套网格空气质量模式预报系统(NAQPMS)对珠江三角洲(简称珠三角)地区2017年9月21日至11月20日的VOCs开展了模拟试验,并利用光化学监测网8个地面站点的VOCs浓度监测数据,对模式模拟的关键VOCs组分进行了精度评估。结果发现,模式对强活性的甲苯、乙烯和二甲苯具有较高的模拟精度,模拟浓度偏差百分比为0.4%~26.6%,模拟能较好再现其日均浓度变化趋势和日变化的双峰特征。但是模式对化学反应活性强且与植物排放密切相关的异戊二烯具有很大的模拟偏差,偏差比近100%,无法再现其白天浓度高、夜间浓度低的观测日变化特征。通过分析发现,现有模拟系统主要考虑人为污染物排放而未考虑生物源排放,可能是导致这一模拟偏差的关键原因。同时,评估结果也表明模式在VOCs空间分布模拟上仍面临很大的不确定性。本文结果揭示了珠三角VOCs模拟面临的关键不确定性,表明融合VOCs观测数据来揭示并减小VOCs模拟的不确定性具有非常迫切的需求。

关键词：挥发性有机物模拟评估地面监测珠三角

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