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Response of phytoplankton functional group to spring drought in a large subtropical reservoir

Journal of Oceanology and Limnology 2024年第5期42卷 1586-1596页

作者： Yue WU qi YE Siwen CHEN Kaining CHEN Shuzhan MA Yiheng CHANG Xiaoli shi State Key Laboratory of Lake Science and Environment Nanjing Institute of Geography and LimnologyChinese Academy of SciencesNanjing 210008China University of Chinese Academy of Sciences Beijing 100049China State Environmental Protection Key Laboratory of Aquatic Ecosystem Health in the Middle and Lower Reaches of Yangtze River Jiangsu Provincial Academy of Environmental ScienceNanjing 210036China

Global warming has caused an increase in the frequency and duration of droughts *** could trigger large changes in physico-chemical conditions and phytoplankton community in waterbodies,resulting in a shift in the phytoplankton *** diatom blooms in reservoirs have been increasingly observed in the past decade in the Taihu Lake *** aim of the present study is to elucidate the impacts of droughts on aquatic environment and to determine the driving factors for the succession of the phytoplankton functional groups based on the analysis of data collected during spring from 2009 to 2020 in the Daxi *** unimodal relationship between 1-month aggregated precipitation index and phytoplankton species richness indicated the competitive exclusion occurred in extremely drought *** structural equation modeling indicated that drought-related low water level conditions intensified sediment resuspension,and increased the phosphorus-enriched nonalgal turbidity in the Daxi ***,a steady shift in the Reynolds phytoplankton functional groups from L 0,TD,J,X 2,and A(phytoplankton taxa preferring low turbidity and nutrient conditions)to TB(pennate diatoms being adapt to turbid and nutrient-rich conditions)was *** increased TP and non-algal turbidity in addition to the lowered disturbance contribute to the prevalence of Group *** the difficulties in nutrient control,timely water replenishment is often a feasible method of controlling the dominance of harmful algae for reservoir ***,alternative water sources are in high demand for ensuring ecological safety and water availability when dealing with drought.

关键词： drought reservoir sediments resuspension phytoplankton functional group diatom

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Crystal structures of RNA-dependent RNA polymerases from Jingmen tick virus and Alongshan virus

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hLife 2024年第1期2卷 18-31页

作者： Zhenyang Liu qi Peng Pu Han Lu Kuai Jianxun qi Yi shi CAS Key Laboratory of Pathogen Microbiology and Immunology Institute of MicrobiologyChinese Academy of SciencesBeijingChina Beijing Life Science Academy BeijingChina International Institute of Vaccine Research and Innovation Savaid Medical SchoolUniversity of Chinese Academy of SciencesBeijingChina Center for Influenza Research and Early-warning CAS-TWAS Center of Excellence for Emerging Infectious DiseaseChinese Academy of SciencesBeijingChina Research Unit of Adaptive Evolution and Control of Emerging Viruses Chinese Academy of Medical SciencesBeijingChina

Jingmenviruses are a group of flavi-like viruses with segmented genome and have been found in various types of hosts,including humans,cattle,monkeys,bats,rodents,sheep,ticks,mosquitoes and ***,including the Jingmen tick virus(JMTV)and Alongshan virus(ALSV),have been associated with febrile illness and flu-like symptoms in *** polymerase plays critical roles in genome replication and transcription and is an ideal target for antiviral ***,we determined the crystal structures of RNA-dependent RNA polymerase(RdRp)domains of JMTV and ALSV at 2.6Åand 3.2Åresolutions,*** overall structures of JMTV and ALSV RdRp domains are similar to those from the typical unsegmented viruses in Flaviviridae family,especially the Flavivirus *** and ALSV RdRps can be divided into three subdomains and the catalytical Motif A-G are conserved like the typical flaviviruses,whereas the zinc-binding pockets are absent from the JMTV and ALSV *** 50-ends of jingmenvirus genomes are varied in length and sequence,and a highly conserved 8-nucleotide element located on the tip of stem loop A was identified and shown to be required for binding with RdRp and performing de novo replication *** findings provide important structural insights into RdRp of segmented flavivirus and reveal the key region of virus genome responsible for replication initiation,which would promote molecular understanding of segmented flavivirus replication and the structure-based design of antiviral drugs against flaviviruses.

关键词： Jingmenviruses X-rays RNA-dependent RNA polymerase replication

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Short lifespan and ‘prime period’ of carbon sequestration call for multi-ages in dryland tree plantations

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Forest Ecosystems 2024年第5期11卷 670-676页

作者： Chongyang Xu Xiuchen Wu Yuhong Tian Liang shi Yang qi Jingjing Zhang Hongyan Liu College of Urban and Environmental Sciences and MOE Laboratory for Earth Surface Processes Peking UniversityBeijing100871China College of Resources Science and Technology Beijing Normal UniversityBeijing100875China Institute of Geographic Sciences and Natural Resources Research Chinese Academy of SciencesBeijing100101China College of JunCao Science and Ecology Fujian Agriculture and Forestry UniversityFuzhou350002China

Enhancing forest cover is important for effective climate change *** suggest that drylands are promising areas for expanding forests,but conflicts arise with increased forest area and water *** tree mortality in drylands raises concerns about carbon sequestration potential in tree *** Chinese dryland tree plantations as an example,we compared their growth with natural *** results suggested plantation trees grew 1.6–2.1 times faster in juvenile phases,significantly shortening time to maturity(13.5 vs.30 years)compared to natural forests,potentially stemming from simple plantation age *** from natural forests,74%of trees in plantations faced growth decline,indicating a short“prime period”for carbon sequestration and even a short ***,a negative relationship between evapotranspiration and tree growth was observed in tree plantations since maturity,leading to high sensitivities of trees to vapor pressure deficit and soil ***,this was not observed in natural *** address this,we suggest afforestation in drylands should consider complex age structures,ensuring a longer prime period for carbon sequestration and life expectancy in tree plantations.

关键词： Tree growth Drylands Tree plantations Carbon sequestration Lifespan

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Hydromassage of macular hole edges for large and persistent full-thickness macular holes

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International Journal of Ophthalmology(English edition) 2024年第3期17卷 551-557页

作者： Yi Cai Wen-Bo Liu duo Wei Xun Deng Xiao-Xin Li Ming-Wei Zhao Xuan shi Jian-Hong Liang Department of Ophthalmology Peking University People’s HospitalBeijing 100044China Eye Diseases and Optometry Institute Beijing 100044China Beijing Key Laboratory of Diagnosis and Therapy of Retinal and Choroid Diseases Beijing 100044China College of Optometry Peking University Health Science CenterBeijing 100044China Xiamen Eye Center of Xiamen University Xiamen 361102Fujian ProvinceChina Department of Ophthalmology the Second Hospital of Lanzhou UniversityLanzhou 730030Gansu ProvinceChina

●AIM:To introduce the macular hole(MH)hydromassage technique as a potentially beneficial approach for the treatment of large or persistent MH.●METHODS:This retrospective observational case series comprised 16 consecutive patients(17 eyes)diagnosed with *** criteria involved a hole aperture diameter larger than 600μm or the presence of an unclosed MH larger than 600μm following the previous *** MH repair procedures were administered in all cases,involving the manipulation and aspiration of the hole margin through the application of water flow with a soft-tip flute needle.A comprehensive assessment was conducted for each case before and after surgery,and optical coherence tomography(OCT)images were captured at every follow-up point.●RESULTS:The mean preoperative aperture diameter was 747±156μm(range 611-1180μm),with a mean base diameter of 1390±435μm(range 578-2220μm).Following surgery,all cases achieved complete anatomical closure of MH,with 13 cases(76.5%)exhibiting type 1 closure and 4 cases(23.5%)demonstrating type 2 *** significant differences were observed in the preoperative OCT variables between the two closure *** with type 1 closure showed a significantly improved visual acuity(0.70±0.10,range 0.50-0.80)compared to those with type 2 closure(0.90±0.12,range 0.80-1.00,P=0.014).●CONCLUSION:The MH hydromassage technique demonstrates promising results,achieving acceptable closure rates in cases of large or persistent *** technique may serve as an effective adjunctive maneuver during challenging MH surgery.

关键词： macular hole large macular hole persistent macular hole optical coherence tomography surgical technique hydromassage

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Investigating Load Regulation Characteristics of a Wind-PV-Coal Storage Multi-Power Generation System

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Energy Engineering 2024年第4期121卷 913-932页

作者： Zhongping Liu Enhui Sun Jiahao shi Lei Zhang qi Wang Jiali Dong Sinopec Star Co. Ltd.Zhengzhou450000China Hebei Key Laboratory of Low Carbon and High Efficiency Power Generation Technology North China Electric Power UniversityBaoding071003China

There is a growing need to explore the potential of coal-fired power plants(CFPPs)to enhance the utilization rate of wind power(wind)and photovoltaic power(PV)in the green energy *** study developed a load regulation model for a multi-power generation system comprising wind,PV,and coal energy storage using realworld *** power supply process was divided into eight fundamental load regulation scenarios,elucidating the influence of each scenario on load *** the framework of the multi-power generation system with the wind(50 MW)and PV(50 MW)alongside a CFPP(330 MW),a lithium-iron phosphate energy storage system(LIPBESS)was integrated to improve the system’s load regulation *** energy storage operation strategy was formulated based on the charging and discharging priority of the LIPBESS for each basic scenario and the charging and discharging load calculation method of LIPBESS auxiliary *** optimization using the particle swarm algorithm,the optimal capacity of LIPBESS was determined to be within the 5.24-4.88 MWh *** an economic perspective,the LIPBESS operating with CFPP as the regulating power source was 49.1% lower in capacity compared to the renewable energy-based storage mode.

关键词： Wind power coal-fired power PV multi-power generation system lithium-iron phosphate energy storage system

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Effects of high-pressure activated slightly acidic electrolyzed water on cleaning and sterilization of pig transfer vehicles

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International Journal of Agricultural and Biological Engineering 2024年第4期17卷 53-59页

作者： Xuedong Zhao Shengnan Tao Fei qi Hao Li Zhengxiang shi Department of Agricultural Structure and Bioenvironmental Engineering College of Water Resources&Civil EngineeringChina Agricultural UniversityBeijing 100083China Key Laboratory of Agricultural Engineering in Structure and Environment Ministry of Agriculture and Rural AffairsBeijing 100083China Beijing Engineering Research Center on Animal Healthy Environment Beijing 100083China

The establishment of biosafety system is of enormous importance to the livestock and poultry production in terms of mitigating the transmission of diseases and implementing regional prevention and control ***,the current sterilization technology presents several drawbacks,including time-consuming procedures,chemical residues,and challenges in treating the sewage after *** this study,a novel cleaning and sterilization method that combines slightly acidic electrolyzed water and high pressure water-jet was *** orthogonal test was conducted to examine the correlation between high-pressure conditions and the various non-structural parameters on the efficacy of sterilization *** a field test,the effectiveness of the technology in cleaning pig transfer vehicles was evaluated by the total plate count and variations of community *** findings revealed that the combination of process parameters,including an available chlorine concentration of 200 mg/L,rinsing pressure of 170 bar,rinsing duration of 10 s,and residence time of 15 min,resulted in a removal rate of colony concentration on the surface of pig transfer vehicles of(96.50±0.91)%.Moreover,it was demonstrated to effectively inhibit a variety of pathogenic *** innovative cleaning system has the potential to replace traditional methods and reduces pollution while saving time and *** introduces a novel approach for sterilization of transportation in livestock and poultry farms as well as the biosafety construction of the animal husbandry.

关键词： slightly acidic electrolyzed water high pressure water-jet sterilization biosafety concentrated animal production

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Punicalagin prevents obesity-related cardiac dysfunction through promoting DNA demethylation in mice

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Food Science and Human Wellness 2024年第3期13卷 1465-1474页

作者： Shengjie Pei Run Liu qingqing Ma Peng Jiang Xin He Zhongshi qi Jiacheng Fang Xu Yang Zirui Yao Xiaoqian Liu Xianfeng Jing Lei Chen duo Li Institute of Nutrition&Health Qingdao UniversityQingdao 266071China School of Public Health Qingdao UniversityQingdao 266071China Central Laboratory Guizhou Aerospace HospitalZunyi 563000China Health Centers of Licha Town Qingdao 266071China College of Computer Science and Technology Qingdao UniversityQingdao 266071China

The aim of this study was to investigate whether punicalagin(PU)could prevent obesity-related cardiac dysfunction by promoting DNA demethy lation,and to explore its possible mechanism.C57BL/6J mice were fed with standard diet,high-fat diet(HFD),HFD supplemented with resveratrol,low-dose PU(LPU)and high-dose PU(HPU)for 8 *** with HFD group,body weight was significantly lower in PU treatment groups,number of cardionwocytes and the protein level of myosin heavy chain 7B were significantly higher in PU treatment *** of 5-hydroxymethylcytosine and 5-formylcytosine were significantly lower in HFD group than in other *** with the HFD group,the protein level of ten-eleven translocation enzyme(TET)2 was significantly higher in PU treatment groups,p-AMP-activated protein kinase(AMPK)was significantly higher in LPU *** of total antioxidant capacity and the protein levels of complexesⅡ/Ⅲ/Ⅴ,oxoglutarate dehydrogenase,succinate dehydrogenase B and fumarate hdrolase were significantly lower in HFD group than PU treatment *** ratio of(succinic acid+fumaric acid)/a-ketoglutarate was significantly higher in HFD group than other *** conclusion,PU up-regulated TETs enzyme activities and TET2 protein stability through alleviating mitochondrial dysfunction and activating AMPK,so as to promote DNA demethylation,thus preventing obesity-related cardiac dysfunction.

关键词： DNA demethylation Mitochondrial function Obesity-related cardiac dysfunction Punicalagin Ten-eleven translocation family enzymes

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Jianpi Decoction Combined with Medroxyprogesterone Acetate Alleviates Cancer Cachexia and Prevents Muscle Atrophy by Directly Inhibiting E3 Ubiquitin Ligase

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Chinese Journal of Integrative Medicine 2024年第6期30卷 499-506页

作者： LI qi KONG Zhao-di WANG Huan GU Hong-hui CHEN Zhong Li shi-guang CHEN Yi-qi CAI Yu YANG Zhen-jiang Department of Oncology and Hematology Shenzhen Traditional Chinese Medicine HospitalShenzhenGuangdong Province518033China College of Pharmacy/International Cooperative Laboratory of Traditional Chinese Medicine Modernization and Innovative Drug Development of Ministry of Education(MOE)of China Jinan UniversityGuangzhou510632China Guangdong Key Lab of Traditional Chinese Medicine Information Technology Jinan UniversityGuangzhou510632China

ObjectiveTo provide comprehensive evidence for the anti-cancer cachexia effect of Jianpi Decoction(JP)and to explore its mechanism of anti-cancer *** mouse model of colon cancer(CT26)-induced cancer cachexia(CC)was used to investigate the anti-CC effect of JP combined with medroxyprogesterone acetate(MPA).Thirty-six mice were equally divided into 6 groups:normal control,CC,MPA(100 mg·kg^(−1)·d^(−1)),MPA+low-dose(20 mg·kg^(−1)·d^(−1))JP(L-JP),MPA+medium-dose(30 mg·kg^(−1)·d^(−1))JP(M-JP),and MPA+high-dose(40 mg·kg^(−1)·d^(−1))JP(H-JP)*** successful modeling,the mice were administered by gavage for 11 *** body weight and tumor volume were measured and recorded every 2 d starting on the 8th day after *** liver,heart,spleen,lung,kidney,tumor and gastrocnemius muscle of mice were collected and *** pathological changes of the tumor was observed,and the cross-sectional area of the gastrocnemius muscle was *** protein expressions of STAT3 and E3 ubiquitinase in the gastrocnemius muscle were measured by Western *** addition,an in vitro C2C12 myotube formation model was established to investigate the role of JP in hindering dexamethasone-induced muscle *** vitro experiments were divided into control,model,and JP serum *** 2-d administration,microscopic photographs were taken and myotube diameters were *** blot was performed to measure the protein expressions of STAT3 and E3 *** combined with MPA restored tumor-induced weight loss(P<0.05,***)and muscle fiber size(P<0.01,***).Mechanistically,JP reduced the expression of atrophy-related proteins MuRF1 and MAFbx in tumor-induced muscle atrophy in vivo(P<0.05,***).In addition,JP reduced the expression of atrophy-related proteins MuRF1 and MAFbx and p-STAT3 phosphorylation(P<0.05 or P<0.01 *** group)in C2C12 myotubes treated with dexamethasone in *** of JP combined with MPA restores t

关键词： cancer cachexia muscle atrophy Jianpi Decoction Chinese medicine E3 ubiquitin ligases

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Interpretation and characterization of rate of penetration intelligent prediction model

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Petroleum Science 2024年第1期21卷 582-596页

作者： Zhi-Jun Pei Xian-Zhi Song Hai-Tao Wang Yi-qi shi Shou-Ceng Tian Gen-Sheng Li College of Petroleum Engineering China University of Petroleum(Beijing)Beijing102249China State Key Laboratory of Petroleum Resources and Prospecting Beijing102249China Kunlun Digital Technology Co. Ltd.Beijing102206China

Accurate prediction of the rate of penetration(ROP)is significant for drilling *** the intelligent ROP prediction model based on fully connected neural networks(FNN)outperforms traditional ROP equations and machine learning algorithms,its lack of interpretability undermines its *** study proposes a novel interpretation and characterization method for the FNN ROP prediction model using the Rectified Linear Unit(ReLU)activation *** leveraging the derivative of the ReLU function,the FNN function calculation process is transformed into vector *** FNN model is linearly characterized through further simplification,enabling its interpretation and *** proposed method is applied in ROP prediction scenarios using drilling data from three vertical wells in the Tarim *** results demonstrate that the FNN ROP prediction model with ReLU as the activation function performs exceptionally *** relative activation frequency curve of hidden layer neurons aids in analyzing the overfitting of the FNN ROP model and determining drilling data *** the well sections with similar drilling data,averaging the weight parameters enables linear characterization of the FNN ROP prediction model,leading to the establishment of a corresponding linear representation ***,the quantitative analysis of each feature's influence on ROP facilitates the proposal of drilling parameter optimization schemes for the current well *** established linear characterization equation exhibits high precision,strong stability,and adaptability through the application and validation across multiple well sections.

关键词： Fully connected neural network Explainable artificial intelligence Rate of penetration ReLU active function Deep learning Machine learning

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Optimizing 2D-metal contact in layered Tin-selenide via native oxide modulation

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Nano Research 2024年第4期17卷 3014-3020页

作者： Yue Zheng qi You Zhentian Yin Jian Tang Ke Jiang Zihao Xie Henan Li Cheng Han Yumeng shi International Collaborative Laboratory of 2D Materials for Optoelectronics Science and Technology of Ministry of Education Institute of Microscale OptoelectronicsShenzhen UniversityShenzhen 518060China College of Electronics and Information Engineering Shenzhen UniversityShenzhen 518060China

The discovery of two-dimensional(2D)semiconductor has opened up new avenues for the development of short-channel field-effect transistors(FETs)with desired electrical *** them,orthorhombic tin-selenide(SnSe)has garnered increasing attention due to its potential applications in a variety of electronic,optoelectronic,and thermoelectric ***,the realization of high-performance SnSe FETs with low contact resistance(Rc)remains a ***,we systematically investigate the contact of few-layer SnSe FETs through the modulation of native oxide on SnSe by using different *** is found that chromium(Cr)-contacted devices possess the best FET performance,such as electron mobility up to 606 cm^(2)/(V·s)at 78 K,current on/off ratio exceeding 1010,and saturation current of~550μA/μm,where a negligible Schottky barrier(SB)of~30 meV and a low contact resistance of~425Ωμm are achieved.X-ray photoelectron spectroscopy(XPS)and cross-sectional electron dispersive X-ray spectroscopy(EDX)results further reveal that the improved contact arises from the Cr-induced reduction of native oxide(SnOx)to Sn,which thins the tunneling barrier for efficient electron *** findings provide a deep insight into the 2D-metal contact of SnSe and pave the way for its applications in future nanoelectronics.

关键词： few-layer SnSe field-effect transistors(FETs) Cr contact native oxide contact resistance(Rc) Schottky barrier(SB)

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