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人血白蛋白及D-氨基半乳糖、脂多糖联合诱导建立大鼠慢加急性肝衰竭模型

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中华肝脏病杂志 2007年第10期15卷 771-775页

作者：刘旭华陈煜王泰龄闾军张立洁宋晨朝张晶段钟平首都医科大学附属北京佑安医院 100069 北京中日友好医院

目的建立与人慢加急性肝衰竭病理过程、生化改变相似,实用性、重复性好的动物模型,为研究慢加急性肝衰竭发生的病理生理机制、药物筛选及疗效评价提供适宜的模型。方法用人血清白蛋白免疫诱导建立肝纤维化大鼠模型,至肝纤维化达S4时予... 详细信息

目的建立与人慢加急性肝衰竭病理过程、生化改变相似,实用性、重复性好的动物模型,为研究慢加急性肝衰竭发生的病理生理机制、药物筛选及疗效评价提供适宜的模型。方法用人血清白蛋白免疫诱导建立肝纤维化大鼠模型,至肝纤维化达S4时予以D-氨基半乳糖与脂多糖联合攻击,计算动物病死率及生存时间,动态观察给药后4、8、12h肝功能、血浆细胞因子水平及病理变化,并以TUNEL法检测原位细胞凋亡,计算凋亡指数。结果人血白蛋白攻击6周时绝大多数大鼠形成经典肝硬化或重度肝纤维化。D-氨基半乳糖与脂多糖联合同时腹腔注射后90%大鼠死于肝衰竭,平均生存时间（16.1±3.7）h,病理表现为肝硬化再生结节内发生大块或亚大块坏死,纤维间隔保留。转氨酶及胆红素的变化符合肝细胞大片坏死时的功能改变,血清TNFα明显增高并与凋亡程度相一致。IL-10随给药时间延长而增高,与临床慢加急性肝衰竭患者变化相似。结论对人血白蛋白免疫诱导型肝硬化及肝纤维化大鼠给予D-氨基半乳糖/脂多糖联合急性攻击可建立慢加急性肝衰竭模型,本实验模拟了临床经常遇到的慢性肝病基础之上发生急性肝衰竭的部分病理生理过程。TNFα介导的肝细胞凋亡可能是该慢加急性肝衰竭重要病理机制之一。

关键词：肝功能衰竭大鼠模型,动物脂多糖类 D-氨基半乳糖

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浅述循证医学在肝脏病学教学和科研中的应用

中华医学教育杂志 2006年第3期26卷 48-49页

作者：张晶段钟平李亚明韩大康首都医科大学附属北京佑安医院人工肝治疗培训中心 100054

循证医学意指“遵循证据的医学”,是国外占主导地位的临床思维方式,近年来引进国内后,逐渐被国内医学界所接受,但是,临床上占主导地位的仍旧是经验医学。以重症肝病的治疗为例,不同的医生根据各自的临床经验选择的治疗方案是截然不同的... 详细信息

循证医学意指“遵循证据的医学”,是国外占主导地位的临床思维方式,近年来引进国内后,逐渐被国内医学界所接受,但是,临床上占主导地位的仍旧是经验医学。以重症肝病的治疗为例,不同的医生根据各自的临床经验选择的治疗方案是截然不同的。在这种情况下,只有循证医学才能够为治疗方案的选择提供最充分的证据。引导学生学习循证医学的工作方法,加强学生循证医学的思想,既有助于提高学生的临床工作水平,也教会了学生自我学习的方法,同时,对学生进行高水平的临床科研也具有重要的指导意义。

关键词：循证医学肝脏病学教学方法科研能力

慢加急性肝衰竭大鼠血清TNF-α、白介素-10及肝细胞凋亡的动态变化

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中国血液净化 2007年第9期6卷 496-499页

作者：张文中刘旭华赵军陈煜段钟平首都医科大学附属北京佑安医院北京100069

目的建立大鼠慢加急性肝衰竭实验模型,观察其血清TNF-α、IL-10及肝细胞凋亡的动态变化。方法首先用人血白蛋白建立大鼠肝硬化模型,至肝纤维化4级时予D-氨基半乳糖/脂多糖急性攻击,计算动物死亡率及生存时间,观察给药后4h、8h、12h肝功... 详细信息

目的建立大鼠慢加急性肝衰竭实验模型,观察其血清TNF-α、IL-10及肝细胞凋亡的动态变化。方法首先用人血白蛋白建立大鼠肝硬化模型,至肝纤维化4级时予D-氨基半乳糖/脂多糖急性攻击,计算动物死亡率及生存时间,观察给药后4h、8h、12h肝功能、血清TNF-α、IL-10水平及病理变化,TUNEL法检测原位细胞凋亡,计算凋亡指数。结果D-氨基半乳糖/脂多糖攻击后90%大鼠死于急性肝衰竭,平均生存时间(16.1±3.7)h,转氨酶及胆红素的变化符合肝功能衰竭特点,病理表现为肝硬化再生结节内发生大块或亚大块坏死,纤维间隔保留。血清TNF-α明显增高并与肝细胞凋亡程度相一致。IL-10随给药时间延长而增高,与临床慢加急性肝衰竭患者变化相似。结论对人血白蛋白免疫诱导型肝硬化大鼠给予D-氨基半乳糖/脂多糖联合急性攻击可建立慢加急性肝衰竭模型。TNF-α介导的肝细胞凋亡可能是该慢加急性肝衰竭重要病理机制之一,提示早期给予人工肝及血液净化治疗清除炎性介质、阻断炎症反应可能对慢加急性肝衰竭起到一定防治作用。

关键词：慢加急性肝衰竭动物模型脂多糖 D-氨基半乳糖肿瘤坏死因子-α

血浆置换联合血液滤过治疗对增殖细胞核抗原表达的影响

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中国血液净化 2006年第7期5卷 373-376页

作者：虞岱斌李亚明段钟平首都医科大学附属北京佑安医院人工肝中心北京100054

目的观察血浆置换联合血液滤过治疗对猪急性肝衰竭肝组织中增殖细胞核抗原(prol-iferatingcellnuclearantigen,PCNA)表达的影响。方法将中国实验小型猪12头随机分为两组,即对照组(6头)和治疗组(6头),两组均给予1.2g/kg体重的D-氨基半乳... 详细信息

目的观察血浆置换联合血液滤过治疗对猪急性肝衰竭肝组织中增殖细胞核抗原(prol-iferatingcellnuclearantigen,PCNA)表达的影响。方法将中国实验小型猪12头随机分为两组,即对照组(6头)和治疗组(6头),两组均给予1.2g/kg体重的D-氨基半乳糖诱导建立药物性急性肝功能衰竭模型。治疗组于给药后24h及48h应用血浆置换联合血液滤过治疗,对照组未给予任何治疗。分别留取给药前、给药后6h、24h、48h、72h动物的肝穿组织,用免疫组织化学技术检测肝组织中PCNA的表达,观察比较肝衰竭过程中不同时间、治疗前后PCNA表达的变化。结果对照组动物肝组织中PCNA表达在模型建立成功后第6h即开始升高,48小时明显升高,此后又急剧下降。治疗组在治疗后PCNA表达明显高于对照组(P<0.05),差异有显著性。结论急性肝衰竭时,残存肝细胞仍有再生能力,血浆置换联合血液滤过治疗可以提高PCNA的阳性表达。

关键词：增殖细胞核抗原急性肝衰竭人工肝

CO hydrogenation conversion driven by micro-environments of active sites over iron carbide catalysts

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Green Energy & Environment 2025年第2期10卷 367-373页

作者： Nan Song Xingxing Li Ebtihal Abograin Wenyao Chen Junbo Cao Jing Zhang De Chen Xuezhi duan Xinggui Zhou State Key Laboratory of Chemical Engineering East China University of Science and Technology130 Meilong RoadShanghai200237China Department of Chemical Engineering Norwegian University of Science and TechnologyTrondheim7491Norway

Essentially clearing the structure-activity relationship between iron carbide catalysts involving multiple active centers to understand the reaction mechanism of CO hydrogenation conversion process is still a great ***,two main micro-environment factors,namely electronic properties and geometrical effects were found to have an integrated effect on the mechanism of CO hydrogenation conversion,involving active sites on multiple crystal *** Bader charge of the surface Fe atoms on the active sites had a guiding effect on the CO activation pathway,while the spatial configuration of the active sites greatly affected the energy barriers of CO *** the defective surfaces were more conducive to CO activation,the defective sites were not the only sites to dissociate CO,as CO always tended to dissociate in a wider *** synergistic effect of the micro-environment also occurred during the CO conversion *** C atoms on relatively flat configurations were more likely to form methane,while the electronic properties of the active sites could effectively describe the C-C coupling process,as well as distinguish the coupling mechanisms.

关键词： Micro-environment CO hydrogenation conversion Electronic property Local environment Synergistic effect

Cognitive reappraisal improves the social decision-making performance of suicide attempters Author links open overlay panel

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Fundamental Research 2025年第1期5卷 115-123页

作者： Tong Wang Xiaoya Liu Moxin duan Bo Zhang Li An Shuang Liu Dong Ming Academy of Medical Engineering and Translational Medicine Tianjin UniversityTianjin 300072China School of Education Tianjin UniversityTianjin 300072China School of Precision Instruments and Optoelectronics Engineering Tianjin UniversityTianjin 300072China

A growing body of evidence suggests that emotion regulation(ER)plays a crucial role in the decision-making(DM)process of suicide attempters(SA).Cognitive reappraisal(CR),an emotion regulation strategy that reinterprets emotional situations to alter physiological and emotional responses,has been studied ***,its effect on SA is yet to be *** present study attempted to use CR to modulate ER in SA to improve their DM performance,and explore the physiological mechanisms underlying this *** scores under natural responses and after using the CR strategy,as well as behavioral and electroencephalographic(EEG)data from subjects were recorded during the classical DM task-ultimatum game(UG)paradigm.52 patients with psychiatric disorders(including 26 SA and 26 non-suicide attempters)and 22 healthy controls(HC)performed in *** results showed that negative emotional experience scores decreased in all three groups after CR,but SA showed less improvement compared to *** behavioral results showed that acceptance of SA significantly increased after CR in both fair and unfair alternatives in the UG task,suggesting that CR can improve DM performance of ***,we extracted the late-positive potential(LPP)and theta-gamma coupling(TGC)of EEG for *** LPP of SA was significantly higher when facing unfair alternatives than in fair ones,reflecting the fact that SA showed stronger negative emotions in the face of unfair *** addition,SA exhibited TGC diminished in frontotemporal regions when facing unfair allocation schemes,which demonstrated the existence of cognitive impairment in *** study verified the feasibility of CR for the moderation of DM ability in SA and provided new ideas for early intervention of suicidal behavior.

关键词： Suicide attempters Decision-making Ultimatum game Cognitive reappraisal Late-positive potential Theta-gamma coupling

Advances in CRISPR-based gene editing technology and its application in nucleic acid detection

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BIOCELL 2025年第1期49卷 21-43页

作者： LIUJIE CHEN LILI duan JIA LI JUN CHEN duanFANG LIAO NONGYUE HE KAI LI ZHENG HU Translational Medicine Institute the First People’s Hospital of ChenzhouHengyang Medical SchoolUniversity of South ChinaChenzhou423000China The First Clinical College of Xiangnan University The First Affiliated Hospital of Xiangnan UniversityChenzhou423000China National Engineering Research Center of Personalized Diagnostic and Therapeutic Technology Hunan University of Chinese MedicineChangsha410208China State Key Laboratory of Digital Medical Engineering School of Biological Science and Medical EngineeringSoutheast UniversityNanjing210096China

Nucleic acid analysis is a key technique that enables accurate detection of various *** nucleic acid testing typically requires access to specialized laboratories,equipment,and trained personnel,which hinders the widespread use of on-site testing for DNA and RNA ***,integrating gene editing technology with traditional nucleic acid detection methods,especially isothermal amplification technology,can help overcome the limitations associated with on-site *** combination can accomplish precise and swift detection of nucleic acid sequences,offering a robust tool for on-site *** Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR-associated proteins(CRISPR/Cas)technology,which comprises the CRISPR system and Cas effector proteins,is a powerful tool that is advancing the field of nucleic acid ***,Cas12,Cas13,and Cas14 proteins have emerged as straightforward,effective,precise,sensitive,and cost-effective methods for in vitro nucleic acid detection because of their“collateral cleavage”*** combined with the“collateral cleavage”ability of Cas protein and isothermal amplification,CRISPR/Cas systems have great potential to advance nucleic acid *** article summarizes the research progress of different CRISPR/Cas systems and their applications in nucleic acid detection and future perspectives.

关键词： CRISPR/Cas Pathogen Clinical application

Mechanism study of the molluscicide candidate PBQ on Pomacea canaliculata using a viscosity-sensitive fluorescent probe

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Chinese Chemical Letters 2025年第1期36卷 447-452页

作者： Lanyun Zhang Weisi Wang Yu-Qiang Zhao Rui Huang Yuxun Lu Ying Chen Liping duan Ying Zhou Yunnan Characteristic Plant Extraction Laboratory College of Chemical Science and TechnologyYunnan UniversityKunming 650091China National Institute of Parasitic Diseases Chinese Center for Disease Control and PreventionChinese Center for Tropical Diseases ResearchWHO Centre for Tropical DiseasesNational Key Laboratory of Inteligent Tracking and Forecasting for Infectious DiseasesKey Laboratory on Parasite and Vector Biology MinistryofHealthShanghai200025China

PBQ[1-(4-chlorophenyl)-3-(pyridin-3-yl)urea],an enormous potent molluscicide,showed excellent Pomacea canaliculata(***)control activity and low toxicity for other aquatic organisms,but its snail-killing mechanisms are still not fully *** employed an optical method to elucidate PBQ action via a novel fluorescent viscosity probe,*** the viscosity in the test solutions increased,compared with that in pure ethanol,a 54-fold fluorescence intensity enhancement of NCV was observed in 310 cP of 90%***,NCV successfully exhibited a selective fluorescence response towards monensin-induced cellular viscosity changes in HepG2 *** liver,stomach,and foot plantar of the tested snails were frozen and sectioned for fluorescent imaging experiments after the treatment with different PBQ concentrations over various times.A significant fluorescent increase in the snail's liver was observed upon exposure to 0.75 mg/L PBQ for 72 h,which highlighted an increase in *** and eosin(HE)staining further supported PBQ-induced liver damage with a viscosity increase in *** study provides a new rapid optical visualization method to study the killing mechanisms of PBQ and may help discover new chemicals that control snail populations.

关键词： Fluorescence sensor Viscosity Pomacea canaliculata Molluscicide mechanisms Bio-imaging

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奥硝唑导致精神异常1例并文献复习

中国新药与临床杂志 2021年第3期40卷 236-238页

作者：闵光宁席宁陈奕达张力衡武新安段钟平兰州大学第一医院药剂科甘肃兰州730000 首都医科大学附属北京佑安医院疑难肝病及人工肝中心/肝衰竭与人工肝治疗研究北京市重点实验室北京100069 驻马店市中心医院河南驻马店463000

奥硝唑(ornidazole)是继甲硝唑、替硝唑之后的第三代新型硝基咪唑类衍生物,有良好的抗厌氧菌作用和抗原虫作用[1],具有起效快、体内滞留时间长、抗菌活性强等特点。该药物主要作用于厌氧菌、阿米巴原虫、贾第鞭毛虫和毛滴虫细胞的DNA,... 详细信息

奥硝唑(ornidazole)是继甲硝唑、替硝唑之后的第三代新型硝基咪唑类衍生物,有良好的抗厌氧菌作用和抗原虫作用[1],具有起效快、体内滞留时间长、抗菌活性强等特点。该药物主要作用于厌氧菌、阿米巴原虫、贾第鞭毛虫和毛滴虫细胞的DNA,使其螺旋结构断裂或阻滞其转录复制而导致病原体死亡[2]。奥硝唑不良反应主要有恶心、呕吐、腹泻、胃肠道反应及皮肤过敏反应等,严重者可致肝损伤.

关键词：奥硝唑精神异常药物不良反应