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The plant circadian clock regulates autophagy rhythm through transcription factor LUX ARRHYTHMO

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Journal of Integrative Plant Biology 2022年第11期64卷 2135-2149页

作者： Ming-Kang yang Xiao-Jie Zhu Chu-Min Chen Xu Guo Shu-Xuan Xu Ya-Rou Xu Shen-Xiu du Shi Xiao Bernd Mueller-Roeber Wei Huang liang Chen State Key Laboratory for Conservation and Utilization of Subtropical Agro‐Bioresources College of Life SciencesSouth China Agricultural UniversityGuangzhou 510642China Guangdong Laboratory for Lingnan Modern Agriculture Guangzhou 510642China Guangdong Provincial Key Laboratory of Protein Function and Regulation in Agricultural Organisms South China Agricultural UniversityGuangzhou 510642China State Key Laboratory of Biocontrol and Guangdong Key Laboratory of Plant Resources School of Life SciencesSun Yat‐Sen UniversityGuangzhou 510275China Department of Molecular Biology Institute of Biochemistry and BiologyUniversity of Potsdam14476 Potsdam‐GolmGermany Max Planck Institute of Molecular Plant Physiology 14476 Potsdam‐GolmGermany

Autophagy is an evolutionarily conserved degradation pathway in eukaryotes;it plays a critical role in nutritional stress *** circadian clock is an endogenous timekeeping system that generates biological rhythms to adapt to daily changes in the *** evidence indicates that the circadian clock and autophagy are intimately interwoven in ***,the role of the circadian clock in regulating autophagy has been poorly elucidated in ***,we show that autophagy exhibits a robust circadian rhythm in both light/dark cycle(LD)and in constant light(LL)in ***,autophagy rhythm showed a different pattem with a phase-advance shift and a lower amplitude in LL compared to ***,mutation of the transcription factor LUX ARRHYTHMO(LUX)removed autophagy rhythm in LL and led to an enhanced amplitude in *** represses expression of the core autophagy genes ATG2,ATG8 a,and ATG11 by directly binding to their *** analysis revealed that LUX is responsible for improved resistance of plants to carbon starvation,which is dependent on moderate autophagy *** transcriptomic analysis revealed that the autophagy rhythm is ubiquitous in *** together,our findings demonstrate that the LUXmediated circadian clock regulates plant autophagy rhythms.

关键词： autophagy carbon starvation circadian clock LUX rhythm

Recent developments in microfluidic device-based preparation, functionalization, and manipulation of nano- and micro-materials

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Particuology 2019年第4期17卷 1-19页

作者： Guangsheng Luo Le du Yujun Wang Kai Wang The State Key Lab of Chemical Engineering Department of Chemical Engineering Tsinghua University Beijing 100084 China The State Key Laboratory of Chemical Resource Engineering Beijing Key Laboratory of Membrane Science and Technology Beijing University of Chemical Technology Beijing 100029 China

Microfluidic systems provide a powerful platform for preparing,functionalizing,and manipulating nano-/*** this review,we summarize the characteristics of state-of-the-art microfluidic techniques and their application to processing novel nano-/micro-materials with tunable size,shape,morphology,composition,and properties.A variety of synthesis strategies based on novel microfiuidic devices are presented and compared,including their integration with real-time monitoring,external fields,and automatic feedback *** functional nano-/micro-materials with complex structures have been controllably prepared and post-processed to obtain high-quality *** for controlling and tuning physical and chemical properties are also examined in terms of the design and function of microfluidic *** provide an outlook on future developments in the processing of nano-/micro-materials by microfluidic technologies.

关键词： Microfluidics Microchemical systems Nanomaterials Micro-materials Scale-up

Impact performance of BFRP and steel-reinforced concrete beams with different span-to-depth ratios:Numerical and analytical studies

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Science China(Technological Sciences) 2023年第2期66卷 301-319页

作者： LAN YuChang ZHANG RenBo JIN Liu du XiuLi Key Laboratory of Urban Security and Disaster Engineering of Ministry of Education Beijing University of TechnologyBeijing 100124China Department of Civil Engineering Tsinghua UniversityBeijing 100084China

Many studies have focused on reinforced concrete(RC)slender beams,while there is a paucity of studies on deep ***,there is a lack of studies on the impact performance of FRP-reinforced concrete *** this study,a 3-D numerical model was established to explore the impact behavior of the basalt fiber reinforced polymer(BFRP)and steelreinforced concrete beams with different span-to-depth ratios(l0/h).The effects of impact mass and tension reinforcement ratio were analyzed and *** is determined that the impact behavior of the shortest beam with l0/h=1.5 corresponds to punching shear failure,which is distinctive from the other deep *** l0/h exceeds 3.0,the failure mode gradually changes from shear dominant to flexural *** increase in the impact mass leads to a linear increase in the max midspan deflection,the duration of the contact between the hammer and member,and the duration of the dynamic reaction *** max midspan deflection has a negative correlation with the reinforcement *** max reaction force increases with an increase in the reinforcement ratio,which is more evident for higher l0/*** failure mode of the BFRP-reinforced concrete beam is similar to that of the steel-reinforced *** the impact condition,in this study,part of the steel bars yield,whereas BFRP bars exhibit no signs of fracture because of their higher tensile strength and better deformability.A simplified calculational method based on energy is proposed in this study,and the method accurately predicts the residual deflection of the concrete beams with different l0/***,it is established that it is difficult to reproduce the impact response of the deep beams via the singledegree-of-freedom(SDOF)model,which is based on the assumption of the same deflection shape as that of slender ***,a more rational simplified model for deep beams under impact loads is still required.

关键词： impact BFRP-reinforced concrete beam span-to-depth ratio simplified method

Evolution and Domestication Footprints Uncovered from the Genomes of Coix

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Molecular Plant 2020年第2期13卷 295-308页

作者： Hongbing Liu Junpeng Shi Zexi Cai Yumin Huang Menglu Lv Huilong du Qiang Gao Yi Zuo Zhaobin Dong Wei Huang Rui Qin Chengzhi liang Jinsheng Lai Weiwei Jin State Key Laboratory of Plant Physiology and Biochemistry National Maize Improvement CenterKey Laboratory of Crop Heterosis and Utilizationthe Ministry of EducationCollege of Agronomy and BiotechnologyChina Agricultural UniversityBeijing 100193P.R.China Center for Crop Functional Genomics and Molecular Breeding China Agricultural UniversityBeijing 100193P.R.China State Key Laboratory of Agrobiotechnology China Agricultural UniversityBeijing 100193P.R.China State Key Laboratory of Plant Genomics Institute of Genetics and Developmental Biologythe Innovative Academy of Seed DesignChinese Academy of Sciences1 Beichen West Road No.2Beijing 100101P.R.China Key Laboratory for Protection and Application of Special Plant Germplasm in Wuling Area of Hubei Province South-Central University for NationalitiesWuhan 430074P.R.China

Coix lacryma-jobi,a plant species closely related to Zea and Sorghum,is an important food and medicinal crop in ***,no reference genome of this species has been reported,and its exact phylogeny within the Andropogoneae remains ***,we generated a high-quality genome assembly of coix comprising～1.73 Gb with 44485 predicted protein-coding *** found coix to be a typical diploid plant with an overall 1-to-1 syntenic relationship with the Sorghum genome,despite its drastic genome expansion(～2.3-fold)due mainly to the activity of transposable *** analysis revealed that coix diverged with sorghum～10.41 million years ago,which was～1.49 million years later than the divergence between sorghum and *** of 27 additional coix accessions revealed that they could be unambiguously separated into wild relatives and cultivars,and suggested that coix experienced a strong genetic bottleneck,resulting in the loss of about half of the genetic diversity during domestication,even though many traits have remained *** data not only provide novel comparative genomic and evolutionary insights into the Andropogoneae lineage,but also an important resource that will greatly benefit molecular breeding of this important crop.

关键词： Coix lacryma-jobi L. genome comparative genomics domestication

Revealing the contribution of basilar membrane's biological activity to the mechanism of the cochlear phonosensitive amplification

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Applied Mathematics and Mechanics(English Edition) 2023年第5期44卷 823-840页

作者： J.Y.liang Wenjuan YAO Genomic Medicine Institute Lerner Research InstituteCleveland Clinic FoundationClevelandOH 44106U.S.A. Shanghai Institute of Applied Mathematics and Mechanics Shanghai UniversityShanghai 200072China School of Mechanics and Engineering Science Shanghai UniversityShanghai 200072China

Explaining the mechanism of the cochlear active phonosensitive amplification has been a major problem in *** basilar membrane(BM)is the key *** 1960,Nobel Laureate von B′ek′esy first discovered BM's traveling wave *** that time,BM's models only have considered the traveling wave but not the biological ***,a new model considering changes of BM's stiffness in space and time is established based on the immersed boundary method to describe its biological *** not only reproduces the results of traveling wave motion but also explains the mechanization on the generation of traveling *** important discovery is that changes of BM's stiffness in space and time will cause the unstable global resonance,which will induce amplification of sounds in *** important inference is that biological activity shall be included in the application of mechanical principles to the analysis of life,which is the essential difference between biomechanics and general mechanics.

关键词： basilar membrane(BM) biological activity mechanical analysis cochlear phonosensitive amplification

Low-temperature synthesis of nitrogen doped carbon nanotubes as promising catalyst support for methanol oxidation

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Journal of Energy Chemistry 2019年第1期28卷 118-122页

作者： liang liang Meiling Xiao Jianbing Zhu Junjie Ge Changpeng Liu Wei Xing Laboratory of Advanced Power Sources Changchun Institute of Applied Chemistry Chinese Academy of Sciences University of Chinese Academy of Sciences Jilin Province Key Laboratory of Low Carbon Chemical Power Sources StateState Key Laboratory of Electroanalytical Chemistry Changchun Institute of Applied Chemistry Chinese Academy of Sciences

The electrochemical methanol oxidation reaction(MOR) is of paramount importance for direct methanol fuel cell(DMFC) application, where efficient catalysts are required to facilitate the complicated multiple charge transfer process. The catalyst support not only determines the dispersion status of the catalysts particles, but also exerts great influence on the electronic structure of the catalysts, thereby altering its intrinsic activity. Herein, we demonstrated that nitrogen atoms, assisted by the pre-treatment of carbon matrix with oxidants, can be easily doped into carbon nanotubes at low temperature. The obtained nitrogen-doped carbon nanotubes can effectively improve the dispersion of the supported platinum nanoparticles and facilitate the MOR by modifying the electronic structure of platinum atoms,through catalyst-support interaction.

关键词： Methanol oxidation Carbon nanotubes Hydrothermal method Nitrogen doping

Study on Effectiveness of Solitary-Wave-Like Form Based on Overtopping Data from Irregular Wave Tests

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China Ocean Engineering 2024年第4期38卷 701-710页

作者： LI Xiao-liang Huang Gui-biao School of Naval Architecture&Ocean Engineering Jiangsu University of Science and TechnologyZhenjiang 212100China

The previous study conducted by Li(2022)demonstrates that the Goda graph can be adequately represented by a solitary-wave-like form across the entire range of relative water depth,with the exception of the extremely shallow ***,it remains uncertain whether this form is equally effective when applied to test data generated by irregular waves,as the Goda graph was initially developed based on overtopping data from regular wave ***,it is unclear whether this form is suitable for formulating overtopping discharge at composite vertical *** order to address these questions,a 2D overtopping experiment was conducted,incorporating both simple and composite types of vertical walls,with various relative water depths across the entire range,excluding the extremely shallow zone.A novel analysis procedure was developed,which proved to be highly productive and can be considered a general method for data ***,the study yielded two conclusions:(1)the solitary-wave-like form is remarkably effective in formulating overtopping test data generated by irregular waves,regardless of whether the vertical wall is simple or composite,and(2)the resulting formulas exhibit definitely better performance compared with existing formulas.

关键词： solitary-wave-like form overtopping discharge overtopping formula vertical wall

Calibration of discrete element modeling: Scaling laws and dimensionless analysis

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Particuology 2022年第3期20卷 55-62页

作者： Yucang Wang Peter Mora Yunpei liang State Key Laboratory of Coal Mine Disaster Dynamics and Control Chongqing UniversityChongqing 400044China College of Petroleum Engineering and Geosciences King Fahd University of Petroleum and MineralsSaudi Arabia

In this paper,dynamic similarity conditions are derived for discrete element simulations by non-dimensionalising the governing *** conditions must be satisfied so that the numerical model is a good representation of the physical *** a pure mechanical system,if three independent ratios of the corresponding quantities between the two models are set,then the ratios of other quantities must be chosen according to the similarity *** scalability of linear and non-linear contact laws is also *** tests of 3D uni-axial compression are carried out to verify the theoretical *** example is presented to show how to calibrate the model according to laboratory data and similarity ***,it is impossible to reduce computer time by scaling up or down certain parameters and continue to uphold the similarity *** results in this paper provide guidelines to assist discrete element modelers in setting up the model parameters in a physically meaningful way.

关键词： Discrete element method Dimensionless analysis Dynamic similarity Parameter calibration ESyS_Particle

A new insight into promotion action of Co2+ in Ni-diamond composite electrodeposition

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Journal of Materials Science & Technology 2019年第8期35卷 1797-1802页

作者： Haifei Zhou Nan du Jingdong Guo Shuan Liu Zhejiang Electric Power Corporation Research Institute Hangzhou 310014 China Nanchang Hangkong University Nanchang 330063 China Shenyang National Laboratory for Materials Science Institute of Metal Research Chinese Academy of Sciences Shenyang 110016 China Ningbo Institute of Material Technology and Engineering Chinese Academy of Sciences Ningbo 315201 China

A new insight into the promotion action of Co^2+ on both particle and metal deposition in Ni-diamond composite electrodeposition system was analyzed according to electrochemical measurements. The results showed that the addition of Co^2+ made particles content in deposits increased remarkably. The change of particles content in deposits was related inversely to the change of cathodic zero potential with the increase of the concentration of cobalt sulfate. Zero charge potential of cathode was shifted to much more negative region. The negative shift of the zero potential, combining with positive shift of the zeta potential, increased the electrostatic force between the particle-adsorbed metallic cations and the cathode. It not only benefits to the transportation of particles in solution towards cathode, but also shortens their residence time on cathodic surface. Meanwhile, entry of particles is also promoted. For metals deposition,reduction resistance of metallic cations rises greatly and deposition current at cathodic potentiodynamic polarization decreases after cobalt sulfate has been added into electrolyte. These factors are favorable for increasing particles content in deposits. In addition, physical model of diamond particles deposition state before and after the addition of Co^(2+)has been discussed.

关键词： Cobalt sulfate Composite electrodeposition Ni-diamond Zero charge potential Particle content in deposits