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An UHPLC-MS/MS method for simultaneous determination of quercetin 3-O-rutinoside, kaempferol 3-O-rutinoside, isorhamnetin 3-O-rutinoside, bilobalide and ligustrazine in rat plasma, and its application to pharmacokinetic study of Xingxiong injection

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Chinese Journal of Natural Medicines 2017年第9期15卷 710-720页

作者： dou li-li DUAN li GUO Long liU Le-Le ZHANG Yu-Dong li Ping liU E-Hu State Key Laboratory of Natural Medicines China Pharmaceutical University Nanjing 210009 China Holdwarm Pharmaceutical China Co. Ltd. Nanjing 210008 China

The present study was designed to develop and validate a rapid, sensitive, and reliable ultra-high performance liquid chromatography coupled with tandem mass spectrometry(UHPLC-MS/MS) method for the simultaneous determination of five major active constituents in the traditional Chinese medicinal preparation Xingxiong injection(XXI) in rat plasma, including quercetin 3-O-rutinoside(QCR), kaempferol 3-O-rutinoside(KFR), isorhamnetin 3-O-rutinoside(ISR), bilobalide(BB), and ligustrazine(LGT). The plasma samples were pretreated by protein precipitation with acetonitrile. The chromatographic separation was achieved on a Waters Symmetry C18 analytical column(2.1 mm × 100 mm, 3.5 mm) with a mobile phase of 0.1% aqueous formic acid(A)-acetonitrile(B). Quantitation of the five bioactive constituents was achieved. Naringin was used as the internal standard(IS). All the calibration curves showed good linearity(r > 0.996) over the concentration range, with the lowest limit of quantification(LLOQ) between 2-18 ng·m L^(-1). The intraand inter-day accuracy and precision of the analytes were both within acceptable limits. Moreover, satisfactory extraction recoveries(90.92%-104.03%) were obtained by protein precipitation. The validated method was successfully applied to a pharmacokinetic study of XXI in rats after intravenous administration at three doses. The pharmacokinetic parameters of the five compounds varied in a dose-dependent manner within the tested dosage range. The present study was the first report of pharmacokinetic study for XXI.

关键词： UHPLC-MS/MS Xingxiong injection Pharmacokinetics Rat plasma

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Comprehensive analysis of chemical constituents in Xingxiong injection by high performance liquid chromatography coupled with mass spectrometry

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Chinese Journal of Natural Medicines 2015年第9期13卷 711-720页

作者： GUO Long dou li-li DUAN li liU Ke BI Zhi-Ming li Ping liU E-Hu State Key Laboratory of Natural Medicines China Pharmaceutical University

Xingxiong injection(XXI) is a widely used Chinese herbal formula prepared by the folium ginkgo extract and ligustrazine for the treatment of cardiovascular and cerebrovascular diseases. Compared with the pharmacological studies, chemical analysis and quality control studies on this formula are relatively limited. In the present study, a high performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry(HPLC-QTOF MS) method was applied to comprehensive analysis of constituents in XXI. According to the fragmentation rules and previous reports, thirty ginkgo flavonoids, four ginkgo terpene lactones, and one alkaloid were identified. A high performance liquid chromatography coupled with triple quadrupole mass spectrometry(HPLC-QQQ MS) method was then applied to quantify ten major constituents in XXI. The method validation results indicated that the developed method had desirable specificity, linearity, precision and accuracy. The total contents of ginkgo flavonoids were about 22.05-25.51 μg·mL-1 and the ginkgo terpene lactones amounts were about 4.41-8.70 μg·m L-1 in six batches of XXI samples, respectively. Furthermore, cosine ratio algorithm and distance measurements were employed to evaluate the similarity of XXI samples, and the results demonstrated a high-quality consistency. This work could provide comprehensive information on the quality control of Xingxiong injection, which be helpful in the establishment of a rational quality control standard.

关键词： Xingxiong injection Favonoids Terpene lactones HPLC-QTOF MS HPLC-QQQ MS

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Comparison of Fracture Resistance Strength of Six Non-metal Dental Posts under the Oblique Load in Vitro

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口腔医学研究 2008年第1期24卷 24-28页

作者： ZHANG Wen - yun YANG li - dou li Nan LUO Xin - xiao Department of Stomatology Kunming General Hospital 212 Daguan Road Kunming China 650032

目的:测试6种非金属桩钉的抗折性能,以了解不同桩钉的强度,从而指导临床合理选择桩钉。材料:将36件桩钉按种类分为6个实验组(n=6):A组:Parapost fiber(Whaledent Coltene);B组:Aesthetic Plus(Bisco);C组:Cosmopost(Ivoclar Vivadent);D组:Exper-fiber A(NWPU);E组:Exper-fiber B(NWPU);F组:Exper-fiber C(NWPU)。将待测的桩钉固定于特别加工的夹具中,用通用电子力学试验机测试桩钉在135°单端受力时的破坏载荷,对结果进行方差分析和SNK检验。结果:不同种类桩钉的抗折力不同,Exper-fiber A posts和Exper fiber Bposts的抗折力无显著差异,分别是(87.21±3.12)N和(89.26±1.72)N。与其他组相比Exper-fiber C posts的抗折力最高为(99.93±1.97)N。Parapost-fiber组的抗折力最低为(47.07±1.36)N。结论:不同种类非金属桩钉的抗折力不同,临床使用中应根据实际受力情况合理选择。

关键词：牙科材料桩核修复纤维/树脂复合材料力学性能

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Deciphering the role of ultra-low-loaded rhodium in NiFe-MIL-53 for superior oxygen evolution reaction

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Journal of Energy Chemistry 2025年第1期100卷 77-86页

作者： Jinzhi Jia Jinhua Zhang Kailu Guo Lanyue Zhang Gening Du Hao You Junfeng Huang Mudong Tu Hua li Yong Peng Wei dou Cailing Xu State Key Laboratory of Applied Organic Chemistry College of Chemistry and Chemical Engineering School of Materials and Energy Lanzhou University College of Chemistry and Chemical Engineering Luoyang Normal University

Designing highly active and stable electrocatalysts of oxygen evolution reaction （OER） is one of the crucial *** this study,a novel OER electrocatalyst,NiFe-MIL-53 modified with ultra-low rhodium（Rh@NiFe-MIL-53）,is successfully prepared via the hydrothermal ***-situ Raman spectroscopy and electrochemical impedance spectroscopy reveal that the doped Rh accelerates the phase transformation of NiFe-MIL-53 and the in-situ formed Rh@NiFeOOH is the actual active *** importantly,the enhanced reversibility of electrochemical reconstruction between NiFeOOH and NiFe（OH）2after doping Rh is beneficial for improving the electrochemical stability of the catalyst.X-ray photoelectron spectroscopy spectra show the strong electronic interaction between single-atom Rh and Ni/Fe in Rh@***,theoretical calculations confirm that the integration of single-atom Rh into the NiFeOOH successfully reduces the band gap,regulates the d-band center （εd）,accelerates the charge transfer,and optimizes the adsorption behavior of oxygen-containing intermediates,thereby lowering the energy barrier of rate-determining ***,the optimized Rh@NiFe-MIL-53 exhibits excellent OER activity （240 mV） with a small Tafel slope of 48.2 mV dec-1and long-term durability (over1270 h at 10 m A cm-2and 110 h at 200 mA cm-2).This work presents a new perspective on designing highly efficient OER electrocatalysts.

关键词： X ray photoelectron spectroscopy

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The effects of experimental pain on pain-related words recognition under controlled processing and automatic processing

The effects of experimental pain on pain-related words recog...

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中国神经科学学会第九届全国学术会议暨第五次会员代表大会

作者： Yanlin LUO,Yaqi SHI,Xueyan FENG,Xin dou,Yuexiu li,Junfa li~* Department of neurobiology,the Capital Medical University,Beijing 100069,China

＜正＞Aims The effect of experimental moderate pain induced by the tourniquet inflation around the upper limb on the recognition of pain-related words under controlled processing or automatic processing and the difference between them were investigated in this *** 32 healthy college students aged from 18 to 22（21.81±2.24） years old including 16 males and 16 females participated in this *** tourniquet was tied to one side upper limb of subjects 1cm above Cubits horizontal *** tourniquet inflation,all subjects were asked to finish the Dot-probe tasks with five kinds of words（affective,sensory,positive,social threat and neutral） by contralateral *** tourniquet was inflated to 200 mmHg and kept this pressure until subjects completed the Dot-probe *** experiment 1,the words were displayed for 100ms in the Dot-probe task,and the words can’t be perceived by subjects. In experiment 2,the words were displayed for 1000 ms,and the words can be *** reaction time and the error rate of recognition task were recorded,and the reformed McGill Pain Questionnaire（MPQ） was filled by subjects after *** Pain intensity during tasks measured by reformed MPQ showed that moderate pain was induced by tourniquet inflation to 200 mmHg（4.81±1.6）.The data of the reaction time with one factor of displaying time,and another factor of five kinds of words were analyzed by two-way measures analysis of variance（ANOVA）.The results showed that a significant difference was found in the main effect in reaction time between controlled processing and automatic processing（P = 0.027）.Reaction time under automatic processing（481±65 ms） is significantly longer than that of controlled processing（464±70 ms）.Compared automatic processing（476±63 ms） with controlled processing （454±62 ms）,male react more quickly（P = 0.032）.Such difference was not found in ***-way ANOVA for the error rate showed no significant difference in the main effect（P = 0.169）.The data of t

关键词： sex difference tourniquet pain word recognition attentional bias automatic processing

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InNi alloy-induced interface effect promoting the parallel electron transfer: improved photothermal catalytic CO2 hydrogenation

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Science China Chemistry 2025年

作者： Haoyu Zhang Yuhang li Yu Nie Xinyu dou Haodong Ji Xin Tan Jinhua Ye Tao Yu School of Chemical Engineering and Technology Tianjin University Eco-environment and Resource Efficiency Research Laboratory School of Environment and Energy Peking University Shenzhen Graduate School School of Environmental Science and Engineering Tianjin University Advanced Catalytic Materials Research Center School of Materials Science and Engineering Tianjin University Research Center for Solar Driven Carbon Neutrality Hebei University

Photothermal catalytic CO2hydrogenation is an effective means of utilizing carbon ***,it is severely limited in terms of kinetics and ***,it is necessary to meticulously design catalysts to solve this ***,a sandwich structured NiO@InNi/In2O3is designed to intrinsically regulate the direction of photogenerated carriers transfer,resulting in a CO yield of 42.97 mmol g-1h-1(1290.3μmol h-1) with a selectivity near to 100%.InNi alloy favors the collection of photogenerated carriers by the parallel way and enhancement of the adsorption and activation of *** grown on the surface of InNi alloy not only improves the adsorption of H2and provides sufficient H+for the reaction,but also makes InNi alloy more stable during the reaction *** photothermal effect caused by In2O3accelerates the transfer of photogenerated carriers and increases the surface temperature of the catalyst,thereby synergistically promoting the reaction from a kinetic *** work ameliorates the kinetic and thermodynamic limitations of the photothermal catalytic CO2hydrogenation process through rationally designing the electron transfer direction of the sandwich structured catalysts to parallel way.

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原子荧光光谱法测定水系沉积物中的锗的研究

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甘肃冶金 2014年第6期36卷 87-88页

作者：豆丽丽窦向丽甘肃有色地质勘查局兰州矿产勘查院中心实验室甘肃兰州730046

本法采用硝酸、氢氟酸、高氯酸以及磷酸分解后,在25%的磷酸介质中,锗与硼氢化钾反应氢化物气体,以氩气为载气导入电热石英炉,火焰中与氢基碰撞离解为自由原子,在空心阴极灯光源的激发下产生荧光光谱。以锗的浓度为横坐标,荧光峰高值为... 详细信息

本法采用硝酸、氢氟酸、高氯酸以及磷酸分解后,在25%的磷酸介质中,锗与硼氢化钾反应氢化物气体,以氩气为载气导入电热石英炉,火焰中与氢基碰撞离解为自由原子,在空心阴极灯光源的激发下产生荧光光谱。以锗的浓度为横坐标,荧光峰高值为纵坐标绘制工作曲线,从而测出试样中锗的浓度。

关键词：原子荧光光谱法水系沉积物锗

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新布尼亚病毒真核表达载体的构建及表达

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中国人兽共患病学报 2020年第4期36卷 280-284页

作者：窦丽丽陶晓莉王晓芳李婷婷李永刚锦州医科大学基础医学院病原生物学教研室锦州121000

目的探究SFTSV-NSs基因的结构和功能以及病毒在感染细胞过程中与宿主的相互作用,通过构建SFTSV-NSs真核表达载体SFTSV-NSs-FLAG,并将其转染进293细胞内,为进一步研究SFTSV-NSs基因的结构和功能奠定基础。方法利用RT-PCR扩增SFTSV-NSs基... 详细信息

目的探究SFTSV-NSs基因的结构和功能以及病毒在感染细胞过程中与宿主的相互作用,通过构建SFTSV-NSs真核表达载体SFTSV-NSs-FLAG,并将其转染进293细胞内,为进一步研究SFTSV-NSs基因的结构和功能奠定基础。方法利用RT-PCR扩增SFTSV-NSs基因序列并测序,并将纯化得到的SFTSV-NSs cDNA片段连接到pFLAG-CMV-3载体,通过EcoRI/BamHI双酶切鉴定重组质粒。将重组表达体pSFTSV-NSs-FLAG导入293细胞中,共聚焦显微镜下观察SFTSV-NSs在细胞中的定位、Western Blot检测SFTSV-NSs蛋白在293细胞中的表达情况。结果成功克隆测序SFTSV-NSs基因;成功构建SFTSV-NSs真核表达载体pSFTSV-NSs-FLAG,并将其转染到293细胞中。在293细胞中的表达检测结果显示共聚焦显微镜下观察重组体主要集中在细胞质中,并形成类包涵体样结构;且Western Blot检测结果显示SFTSV-NSs蛋白在293细胞中表达。结论为进一步研究SFTSV-NSs基因的结构和功能以及病毒在感染细胞过程中与宿主的相互作用提供新思路。

关键词：新布尼亚病毒载体构建 293细胞表达

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