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Food Science and Human Wellness 2021年第1期10卷 87-93页

作者： Huan Xiang dongxiao sun-waterhouse Chun Cui School of Food Science and Engineering South China University of TechnologyGuangzhou510640GuangdongChina Guangdong Weiwei Biotechnology Co. LTDGuangzhou510640GuangdongChina School of Chemical Sciences The University of AucklandPrivate Bag 92019AucklandNew Zealand

Auricularia auricula(AA)and Auricularia polytricha(AP)are popular edible *** study successfully produced hypoglycemic polysaccharides from un-smashed or smashing and sieving(through a 10-mesh sieve)AA and AP(termed as AAP/AAP-10 and APP/APP-10)via scalable processes(water extraction,ethanolic precipitation and deproteinization).This is the first report to compare the effectiveness of AAP and APP in combating streptozotocin-induced oxidative stress and diabetes-related changes in mice(body weight,fasting blood glucose,serum insulin,proinflammatory mediator and cytokines,oxidative stress-related products,antioxidant enzymes).APP and AAP with different molecular weights and monosaccharide molar ratios could be therapeutic options for diabetes with a low dose(100 mg/kg/day)likely working *** the same dose,APP generally performed more effective than AAP,and AAP-10/APP-10 seemed slightly more beneficial than AAP/*** mechanism underlying these antidiabetic functions might involve the NF-κB and associated signalling *** is cheaper than AA,thereby representing a favorable source of functional polysaccharides.

关键词： Polysaccharides Auricularia auricula Auricularia polytricha Hypoglycemic effect Oxidative stress

A red pomegranate fruit extract-based formula ameliorates anxiety/depression-like behaviors via enhancing serotonin (5-HT) synthesis in C57BL/6 male mice

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Food Science and Human Wellness 2021年第3期10卷 289-296页

作者： Xiping Zhu dongxiao sun-waterhouse Chun Cui College of Food Science and Engineering South China University of TechnologyGuangzhou 510640GuangdongChina College of Biological and Food Engineering Anhui Polytechnic UniversityWuhu 241000AnhuiChina Guangdong Weiwei Biotechnology Co. LTD.Guangzhou 510640China

This study investigated the anti-anxiety/anti-depression potential of a formula containing red pomegranate fruit extract(RPFE;40%,m/m),Lactobacillus rhamnosus(JB-1)(34%,m/m),magnesium gluconate(25%,m/m)and vanillin(1%,m/m).The RPFE formula(dose:2.0,1.5 or 1.0 mg/g·day)reversed behavioral dysfunctions and body weight gain induced by chronic restraint stress combined with corticosterone injection in C57BL/6 male *** RPFE formula exhibited the abilities to normalize the levels of serum infl ammatory cytokines(NF-κB,TNF-α,IL-6,IL-1βand IFN-γ)and malondialdehyde(MDA),and activities of superoxide dismutase(SOD),catalase(CAT)and nitric oxide synthase(NOS),as well as relieve the injury of hippocampal *** serotonin(5-HT)levels in hippocampus were increasingly enhanced,which might be mediated by reducing the activity of indoleamine-2,3-dioxygenase(IDO)and increasing the activity of tryptophan hydroxylase(TPH).Thus,the neuroprotective and ameliorating effects on anxiety/depression-like behaviors resulting from the RPFE formula ingestion were possibly related to serotonergic activation,which might be mediated via anti-infl ammatory and anti-oxidant actions.

关键词： Behavioral tests Red pomegranate fruit extract 5-HT Anti-infl ammatory Anxiety/depression

Engineering local coordination environments and site densities for high-performance Fe-N-C oxygen reduction reaction electrocatalysis

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SmartMat 2021年第2期2卷 154-175页

作者： Qing Wang Lu Shang dongxiao sun-waterhouse Tierui Zhang Geoffrey waterhouse School of Chemical Sciences The University of AucklandAucklandNew Zealand Key Laboratory of Photochemical Conversion and Optoelectronic Materials Technical Institute of Physics and ChemistryChinese Academy of SciencesBeijingChina

Fe-N-C catalysts represent very promising cathode catalysts for polymer electrolyte fuel cells,owing to their outstanding activity for the oxygen reduction reaction(ORR),especially in alkaline *** this review,we summarize recent advances in the design and synthesis of Fe-N-C catalysts rich in highly dispersed FeNx active *** emphasis is placed on emerging strategies for tuning the electronic structure of the Fe atoms to enhance the ORR activity,and also maximizing the surface concentration of FeNx sites that are catalytically accessible during *** great progress has been made over the past 5 years in the development of Fe-N-C catalyst for ORR,significant technical obstacles still need to be overcome to enable the large-scale application of Fe-N-C materials as cathode catalysts in real-world fuel cells.

关键词： Fe-N-C local coordination environment oxygen reduction reaction site density

Pectin fractions extracted sequentially from Cerasus humilis:Their compositions,structures,functional properties and antioxidant activities

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Food Science and Human Wellness 2023年第2期12卷 564-574页

作者： Shikai Zhang Geoffrey INwaterhouse Tingting Cui dongxiao sun-waterhouse Peng Wu College of Food Science and Engineering Shandong Agricultural UniversityTaian 271018China School of Chemical Sciences The University of AucklandPrivate Bag 92019New Zealand Biology Institute Qilu University of Technology(Shandong Academy of Sciences)Jinan 250103China

Three pectin fractions(water-soluble fraction(WSF),chelator-soluble fraction(CSF),and sodium carbonatesoluble fraction(NSF))were obtained from Chinese dwarf cherry(Cerasus humilis)*** of them were branched low methoxylated pectins with an amorphous or partially nanocrystalline nature and eight neutral monosaccharides(arabinose and galactose were most abundant).WSF,CSF and NSF had a degree of methylation(DM)of 35.82%,14.85%and 7.13%,uronic acid(UA)content of 76.02%,83.71%and 69.01%,and total protein content of 2.4%,2.1%and 8.8%,*** molecular weights were 340.31,330.16 and 141.31 kg/mol,respectively(analyzed by gel permeation chromatography(GPC)).WSF,CSF and NSF exhibited good rheological,thermal,emulsifying,emulsion-stabilizing,water-adsorbing,oil-binding,cholesterol-binding and antioxidant *** had the highest emulsifying,emulsion stabilizing,water-/oil-/cholesterol-binding and antioxidant capacities,followed by *** had the highest viscosity(406.77 m Pa·s),flowability,and resistance to heat-induced changes/damage,which may be related to its lowest polydispersity index,DM and UA content and highest protein *** three pectin fractions with desirable characteristics can be used as food additives/ingredients and dietary supplements.

关键词： Cerasus humilis fruits Pectin fractions Structural characteristics Processing-related functional properties

Deep eutectic solvents and alkaline extraction of protein from seabuckthorn seed meal: a comparison study

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Food Science and Human Wellness 2022年第4期11卷 1028-1035页

作者： Junjie Lin Huan Xiang dongxiao sun-waterhouse Chun Cui Wei Wang College of Food Science and Technology South China University of TechnologyGuangzhou 510640China Guangdong Wei-Wei Biotechnology Co. LtdGuangzhou 510640China

Seabuckthorn seed meal(SSM) is a waste of oil extraction industry that rich in protein. In order to seek suitable protein extraction method, three different deep eutectic solvents(DESs)(including choline chlorideglycerol, choline chloride-oxalic acid and choline chloride-urea) were developed for extracting protein from SSM and compared with alkaline. Result indicated that alkaline could effectively extract 56.9% protein from SSM and its protein content was 73.1%, higher than DES at 31.0%-41.4% and 64.3%-67.5%, respectively. However, compared to alkali, DES led to a product with less β-sheet, more β-turn, more essential amino acids, higher total amino acid content, especially choline chloride-urea which extracted protein showing an integrated and similar protein weight distribution compared to SSM. Also, this protein extracted chloride-urea showed a highest digestibility in vitro(by pepsin)(54.2%). These results indicated that choline chloride-urea extraction is better than alkaline extraction for SSM.

关键词： Seabuckthorn seed meal Protein extraction Deep eutectic solvent Alkaline extraction and acid precipitation Amino acids

Transcriptomic analysis of molecular mechanisms underlying the biodegradation of organophosphorus pesticide chlorpyrifos by Lactobacillus delbrueckii *** in skimmed milk

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Food Science and Human Wellness 2024年第5期13卷 3018-3030页

作者： Yue Yang Wenxia Zhou Lingyu Yang Yilun Chen dongxiao sun-waterhouse Dapeng Li Key Laboratory of Food Nutrition and Human Health in Universities of Shandong College of Food Science and EngineeringShandong Agricultural UniversityTai’an 271018China

Bioremediation of organophosphorus pesticides in contaminated foodstuffs using probiotics has been increasingly under the spotlight in recent years,though the biodegradation mechanism and derived intermediate products remain *** study aimed to help fill this knowledge gap and examined the degradation mechanism of organophosphorus pesticide,chlorpyrifos,in milk by Lactobacillus delbrueckii *** using gas chromatography-tandem mass spectrometry(GC-MS/MS)combined with transcriptome *** the strain was cultured for 20 h in the presence of chlorpyrifos,differential expressions of 383 genes were detected,including genes probably implicated during chlorpyrifos degradation such as those related to hydrolase,phosphoesterase,diphosphatase,oxidoreductase,dehydratase,as well as membrane ***-MS/MS analysis revealed the changes of secondary metabolites in *** during milk fermentation due to chlorpyrifos stress.6-Methylhexahydro-2H-azepin-2-one,2,6-dihydroxypyridine and methyl 2-aminooxy-4-methylpentanoate as intermediates,along with the proposed pathways,might be involved in chlorpyrifos biodegradation by ***.

关键词： Lactobacillus delbrueckii ssp.bulgaricus Biodegradation Chlorpyrifos Transcriptomics Metabolome

Fermentation-enabled wellness foods:A fresh perspective

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Food Science and Human Wellness 2019年第3期8卷 203-243页

作者： Huan Xiang dongxiao sun-waterhouse Geoffrey I.N.waterhouse Chun Cui Zheng Ruan South China University of Technology GuangzhouChina School of Chemical Sciences The University of AucklandPrivate Bag 92019AucklandNew Zealand

Fermented foods represent an important segment of current food markets,especially traditional or ethnic food *** demand for efficient utilization of agrowastes,together with advancements in fermentation technologies(microbial-and enzyme-based processing),are stimulating rapid growth and innovation in the fermented food *** addition,the health-promoting benefits of fermented foods are attracting increasingly *** microorganisms contained in many common fermented foods can serve as“microfactories”to generate nutrients and bioactives with specific nutritional and health ***,recent research relating to the manufacture of fermented foods are critically reviewed,placing emphasis on the potential health benefits of fermentation-enabled wellness *** importance of the correct selection of microorganisms and raw materials and the need for precise control of fermentation processes are *** knowledge gaps and obstacles to fermented food production and market penetration are *** importance of integrating multidisciplinary knowledge,communicating with consumers,establishing regulatory frameworks specifically for fermentation-enabled wellness foods and functional fermented foods,are highlighted.

关键词： Fermented foods Microbial factories Bioactive Probiotics Nutrients Processing technologies

Immunoregulatory polysaccharides from Apocynum venetum *** stimulate phagocytosis and cytokine expression via activating the NF-κB/MAPK signaling pathways in RAW264.7 cells

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Food Science and Human Wellness 2022年第4期11卷 806-814页

作者： Honglin Wang Changyang Ma dongxiao sun-waterhouse Jinmei Wang Geoffrey Ivan Neil waterhouse Wenyi Kang National R&D Center for Edible Fungus Processing Technology Henan UniversityKaifeng 475004China Functional Food Engineering Technology Research Center Kaifeng 475004China School of Chemical Sciences University of AucklandAuckland 1142New Zealand Joint International Research Laboratory of Food&Medicine Resource Function Kaifeng 475004China

Two immunomodulatory polysaccharides(Vp2a-Ⅱ and Vp3) were isolated and identified from Apocynum venetum L. flowers, and their innate immune-stimulating functions and working mechanisms were evaluated in RAW264.7 cells. Both the level of released nitric oxide(NO) and expression of inducible nitric oxide synthase(iNOS) m RNA were significantly enhanced in the RAW264.7 macrophages cells treated by Vp2a-Ⅱ and Vp3. Vp2a-Ⅱ(100–800 μg/m L) and Vp3(400 μg/mL) could significantly increase the phagocytic activity of RAW264.7 cells and the secretion and m RNA expression of TNF-α and IL-6 in a concentrationdependent manner through affecting mitogen-activated protein kinase(MAPK) activity and nuclear factor κB(NF-κB) nuclear translocation. Vp2a-Ⅱ might activate the MAPK signaling pathways and induce the nuclear translocation of NF-κB p65, whilst Vp3 likely activated the NF-κB and MAPK signaling pathways without influencing the p38 MAPK route.

关键词： Apocynum venetum L.flowers Immunomodulatory polysaccharide RAW264.7 cells NF-κB signaling pathway MAPK signaling pathway

Structural characteristics,anticoagulant and antithrombotic mechanism of a novel polysaccharide from Rosa Chinensis Flos

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Food Science and Human Wellness 2023年第2期12卷 407-415页

作者： Xiaofeng Zhang Zhenhua Liang Geoffrey Ivan Neil waterhouse Shengjun Jiang dongxiao sun-waterhouse Jinmei Wang Changyang Ma Wenyi Kang National R&D Center for Edible Fungus Processing Technology Henan UniversityKaifeng 475004China Joint International Research Laboratory of Food&Medicine Resource Function Henan ProvinceKaifeng 475004China School of Chemical Sciences University of AucklandAuckland 1142New Zealand Kaifeng Key Laboratory of Functional Components in Health Food Kaifeng 475004China

This is the first report on a polysaccharide(RCJ2-Ib)isolated from Rosa Chinensis ***2-Ib was obtained through the extraction with water,precipitation with ethanol,separation with DEAE-52 column and purification with DEAE-Sepharose Fast Flow column and Sephadex G100 ***,FT-IR and NMR analyses revealed that RCJ2-Ib(3.3 k Da)was a 1,4-linked polymannuronic acid containing substantialβ-Danomers *** anticoagulant effect of RCJ2-Ib evaluated by using rabbit ear venous blood and an acute blood stasis rat model showed that RCJ2-Ib had obvious anticoagulant activity in regulating endogenous and exogenous coagulation pathways and reducing serum thromboxane B2 and *** addition,RCJ2-Ib could also increase the number of Lactobacillus and Escherichia *** a result,RCJ2-Ib has the potential to inhibit thrombosis and maintain the intestinal environment.

关键词： Rosa Chinensis Flos Polysaccharide isolation Antithrombotic activity Intestinal flora

Perspectives on diacylglycerol-induced improvement of insulin sensitivity in type 2 diabetes

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Food Science and Human Wellness 2022年第2期11卷 230-237页

作者： Daoming Li Yang Zhu Yonghua Wang Qiong Zou Jinzhu Duan dongxiao sun-waterhouse Baoguo sun Beijing Advanced Innovation Center for Food Nutrition and Human Health Beijing Technology and Business UniversityBeijing 100048China School of Food and Biological Engineering Shaanxi University of Science and TechnologyXi’an 710021China School of Food Science and Engineering Guangdong Research Center of Lipid Science and Applied Engineering TechnologySouth China University of TechnologyGuangzhou 510640China Bioprocess Engineering Wageningen University and Research6700 AA WageningenNetherlands Guangzhou Yong-h Special Nutrition Technology Co.Ltd Guangzhou 510000China Guangzhou Institute of Pediatrics Guangzhou Women and Children's Medical CenterGuangzhou 510623China

Diacylglycerol(DAG)-based edible oils have attracted increasing research interest owing to their healthpromoting *** animal and human studies showed that an increased 1,2-DAG content in the liver and skeletal muscle may cause insulin ***,earlier studies using animal models or humans reported that dietary DAGs with a 1,2-DAGs to 1,3-D AGs ratio of approximately 3:7 could improve insulin sensitivity in type 2 diabetic *** conflict raises the question of whether there is a link between the ingested DAGs and endogenous DAGs during their *** make a contribution to this field,this review provides an overview of the metabolic pathways of ingested DAGs and biological roles of DAGs(ingested and endogenous)in the change of insulin ***,strategies for further investigations on the metabolism of DAGs are proposed.

关键词： Diacylglycerols Functional oil Insulin sensitivity Lipidomics