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Biosafety and Health 2023年第1期5卷 1-7页

作者： Lei Liang Xinfang Hong Bing Lu Xuedong Yu Guoqing Cao Xin Feng Quan Zhou Yanqin Wang donglai wu Rong Wang China Academy of Building Research Co. Ltd.Beijing 100013China Second Medical Center of PLA General Hospital Beijing 100853China Academy of Military Sciences Beijing 100071China Guangzhou Laboratory Guangzhou 510320China Chinese Academy of Agricultural Sciences Harbin Veterinary Research Institute Harbin 150069China Tianjin Key Laboratory of Indoor Air Environmental Quality Control School of Environmental Science and EngineeringTianjin UniversityTianjin 300072China

In this study,standards of high-biocontainment facilities(including laboratories and large-scale production facilities)formulated by China and other countries were analyzed and compared,and the technical points and requirements for Heating,Ventilating,and Air Conditioning(HVAC)systems in different series of standards were *** main focus was on expounding the design and verification of the containment area’s indoor parameters,ventilation system,filter design,integrity test,fan standby,airflow pattern,and system *** study expects suggestions and opinions on the construction and development of biosafety facilities in China and the possible future revision of relevant national standards.

关键词： High-biocontainment facilities HVAC systems Standards Technical requirements

Basic requirements of dunk tank settings and verification of the disinfection effect in high-level biosafety laboratories

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Biosafety and Health 2020年第3期2卷 120-123页

作者： Dong Wang Baiming Sun Xianfeng Zhang Hailong Tong Xiaoyu Zhang Yunting Bu Weiyu Li Si Chen donglai wu Harbin Veterinary Research Institute Chinese Academy of Agricultural SciencesHarbin 150000China

This study presents the structure and application of dunk tanks in high-level biosafety *** test methods and sterilization effects of dunk tanks were evaluated by using Bacillus subtilis as *** showed that sterilization effects of dunk tanks could be achieved by immersing the goods in 0.5%sodium hypochlorite disinfectant solution for 30 min at room *** sodium thiosulfate had no effect on the growth of Bacillus subtilis,and thus would not affect the disinfection results of sodium ***,suggestions for safe operation of dunk tanks are also provided in this study.

关键词： Biosafety laboratory Dunk tank Disinfection Sodium hypochlorite Sodium thiosulfate Bacillus subtilis Neutralization

Simultaneous photocatalytic degradation and SERS detection of tetracycline with self-sustainable and recyclable ternary PI/TiO_(2)/Ag flexible microfibers

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Microsystems & Nanoengineering 2024年第2期10卷 101-113页

作者： donglai Han Boyang Guo Yanru Li Wei Feng Keyan Liu Tianna wu Yuchun Wan Lili Wang Ming Gao Yang Liu Lili Yang Maobin Wei Shuo Yang School of Materials Science and Engineering Changchun University of Science and Technology130022ChangchunChina College of Science Changchun University130022ChangchunChina Key Laboratory of Functional Materials Physics and Chemistry of the Ministry of Education Jilin Normal University130103ChangchunChina

Facile and efficient photocatalysts using sunlight,as well as fast and sensitive surface-enhanced Raman spectroscopy(SERS)substrates,are urgently needed for practical degradation of tetracycline(TC).To meet these requirements,a new paradigm for PI/TiO_(2)/Ag organic‒inorganic ternary flexible microfibers based on semiconducting titanium dioxide(TiO_(2)),the noble metal silver(Ag)and the conjugated polymer polyimide(PI)was developed by engineering a simple *** sunlight,the photocatalytic characteristics of the PI/TiO_(2)/Ag flexible microfibers containing varying amounts of Ag quantum dots(QDs)were evaluated with photocatalytic degradation of TC in aqueous *** results demonstrated that the amount of Ag affected the photocatalytic *** the tested samples,PI/TiO_(2)/Ag-0.07(93.1%)exhibited a higher photocatalytic degradation rate than PI/TiO_(2)(25.7%),PI/TiO_(2)/Ag-0.05(77.7%),and PI/TiO_(2)/Ag-0.09(63.3%).This observation and evaluation conducted in the present work strongly indicated a charge transfer ***,the PI/TiO_(2)/Ag-0.07 flexible microfibers exhibited highly sensitive SERS detection,as demonstrated by the observation of the Raman peaks for TC even at an extremely low concentration of 10–10 moles per *** excellent photocatalytic performance and SERS detection capability of the PI/TiO_(2)/Ag flexible microfibers arose from the Schottky barrier formed between Ag and TiO_(2)and also from the outstanding plasmonic resonance and visible light absorptivity of Ag,along with immobilization by the *** successful synthesis of PI/TiO_(2)/Ag flexible microfibers holds significant promise for sensitive detection and efficient photocatalytic degradation of antibiotics.

关键词： mechanism. photocatalytic ternary

Comprehensive Mapping of Murine B cell Epitopes on Glycoprotein E2 from Eastern Equine Encephalitis Virus

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Comprehensive Mapping of Murine B cell Epitopes on Glycoprot...

不确定需求下基于分布式鲁棒机会约束的车辆调度问题研究

2012年中国畜牧兽医学会兽医病理学分会暨中国病理生理学会动物病理生理专业委员会学术研讨会

作者： EnCheng Sun Jing Zhao NiHong Tao Yang QingYuan Xu YongLi Qin YinHui Yang donglai wu The Key Laboratory of Veterinary Public Health Ministry of AgricultureState Key Laboratory of Veterinary BiotechnologyHarbin Veterinary Research InstituteChinese Academy of Agricultural Sciences Beijing Institute of Microbiology and Epidemiology

The study was to map the linear B-cell epitopes on Eastern equine encephalitis virus(EEEV) E2 glycoprotein by *** preparation of recombinant EEEV E2 glycoprotein was according to the instructions of the Bac-to-Bac Baculovirus Expression System and Ni-nitrilotriacetic acid affinity chromatography was used for the *** purified recombinant EEEV E2 protein was used as antigen for the generation of E2-specific pAbs and *** E2 protein-reactive antibody titers of the final immunization demonstrated that the titer of pAbs was over 1:10 and 51 hybridomas stably secreting mAbs against E2 protein were developed by indirect ELISA and *** IFA with Sf9 insect cells infected with BACV-E2 showed that all the 51 mAbs could react positively with EEEV E2 protein whereas no reaction was detected on the Sf9 cells infected with wild type ***,the IFA with BHK transfected with pShuttle-E2 demonstrated a different result that 45 mAbs could react positively with EEEV E2 protein while no reaction was found on other 6 *** heavy chain subtypes of 51 mAbs contained IgG1,Ig2a and IgM with only one type light chain,a k light chain. With 42 expression fusion polypeptides with MBP-tag,the result of WB showed that anti-E2 protein murine pAbs could react positively with 15 of the 42 polypeptides and negatively with MBP-tag,that is,15 linear B-cell epitopes of E2 protein through screening with murine pAbs were *** corresponding locations of these epitopes were at 1-16aa,11-26aa,101-116aa,11l-126aa,131-146aa, 151-166aa,171-186aa,201-216aa,231-246aa,24l-256aa,271-286aa,301-316aa,311-326aa,321-336aa, *** further identification,the above 15 polypeptides containing 16 amino acids were synthetized and the peptides ELISA confirmed that the murine pAbs had good reactivity with the above 15 synthetized polypeptides. To identify the epitopes against the prepared mAbs,the ELISA was carried out coating with the 42 expressed polypeptides.9 linea

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系统工程理论与实践 2018年第7期38卷 1792-1801页

作者：冉伦吴东来焦子豪王珊珊袁书宁北京理工大学管理与经济学院

随着新能源汽车共享模式的迅速发展,允许异地还车导致车辆不均衡问题日益突出.本文考虑不确定的车辆需求,基于需求量的均值和方差等部分信息,最小化最坏情况下系统可用车辆和空闲停车位的机会约束,建立分布式鲁棒优化机会约束车辆调度模... 详细信息

随着新能源汽车共享模式的迅速发展,允许异地还车导致车辆不均衡问题日益突出.本文考虑不确定的车辆需求,基于需求量的均值和方差等部分信息,最小化最坏情况下系统可用车辆和空闲停车位的机会约束,建立分布式鲁棒优化机会约束车辆调度模型,以总成本最小化为目标,得出模型的数学性质和易求解的等价形式,确定停车桩之间的车辆调度数量.最后,以北京市15个停车桩的车辆调度为例,确定最优的车辆调度方案.结果表明,随着服务水平的增加,调度成本和车辆调度数量增加明显,当服务水平增加到一定程度后,应增加系统的车辆数和停车位以满足服务水平的要求.决策者可根据自己的偏好及系统的服务要求,选择恰当的服务水平参数组合,以获得最优的车辆调度方案.

关键词：车辆调度分布式鲁棒机会约束需求不确定

车用燃料电池空压机三高环境工作特性试验研究

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时代汽车 2023年第17期 139-141页

作者：吴东来中国汽车工程研究院股份有限公司重庆市401122

空压机是燃料电池发动机空气供应系统的核心部件之一,其性能好坏直接影响燃料电池系统的效率、动态性能、噪声等关键性能指标。高温、高寒、高原条件影响空压机入口的空气特性,进而影响空压机在“三高”环境条件下的工作特性。通过设计... 详细信息

空压机是燃料电池发动机空气供应系统的核心部件之一,其性能好坏直接影响燃料电池系统的效率、动态性能、噪声等关键性能指标。高温、高寒、高原条件影响空压机入口的空气特性,进而影响空压机在“三高”环境条件下的工作特性。通过设计空压机三高环境试验方案并进行试验分析发现:高温条件下,相同转速空压机流量和压比降低,功率相应降低;低温环境下,相同转速空压机的流量和压比增大,功率相应增加;高原条件下,相同转速空压机流量和压比相应减小,功率相应降低。

关键词：燃料电池空压机环境适应性试验研究

绿色金融助推长江经济带发展路径探析——以荆州农商行为例

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长江技术经济 2021年第5期5卷 39-43页

作者：吴东来长江大学经济与管理学院湖北荆州434023

以荆州市农商行实施绿色金融助推长江经济带发展作为切入点,重点阐述荆州农商行助力长江经济带发展的具体措施和实施成效,并对长江经济带发展中绿色金融发展提出相关政策建议。

关键词：绿色金融长江经济带荆州农商行

中国东北地区3株日本脑炎病毒株的分离鉴定及基因分型

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东北农业大学学报 2009年第6期40卷 73-78页

作者：张永欣符芳宋淑萍李曦吴东来东北农业大学动物医学学院哈尔滨150030 中国农科院哈尔滨兽医研究所哈尔滨150001

从黑龙江省某3个猪场采集疑似为日本脑炎病毒感染的病、死猪的脏器以及全血，进行病毒的细胞培养，蚀斑纯化，间接免疫荧光试验，电镜下观察病毒粒子的形态，及RT—PCR检测。结果显示，盲传三代后，出现明显的细胞病变（CPE＋～CPE＋＋... 详细信息

从黑龙江省某3个猪场采集疑似为日本脑炎病毒感染的病、死猪的脏器以及全血，进行病毒的细胞培养，蚀斑纯化，间接免疫荧光试验，电镜下观察病毒粒子的形态，及RT—PCR检测。结果显示，盲传三代后，出现明显的细胞病变（CPE＋～CPE＋＋＋）；荧光显微镜下细胞胞质内呈现不同强度的绿色荧光信号；电镜下的病毒粒子为40-60nm的球型粒子；PCR检测为日本脑炎病毒阳性。通过对3株分离株腑扩增序列比较，此3株分离株为基因Ⅲ型。

关键词： JEV 蚀斑间接免疫荧光电镜 RT-PCR

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基于PLC的轮机自动化实验平台开发

中国现代教育装备 2017年第7期 13-15页

作者：吴金波杨泽玉巫东来华中科技大学船舶与海洋工程学院湖北武汉430074

基于PLC搭建了轮机自动化多功能实验平台。该实验平台由液位控制、温度控制、运动控制、电机启动和保护四个实验组成。通过该平台,学生可以自主编写PLC程序并进行实验。

关键词：轮机自动化 PLC 实验教学

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支流洪水组合对干流区域的致灾影响模拟分析

应用基础与工程科学学报 2024年第2期32卷 394-409页

作者：吕佳豪侯精明史超李东来陈光照吴新垒西安理工大学省部共建西北旱区生态水利国家重点实验室陕西西安710048 宁波市海曙区水旱灾害防御中心浙江宁波315000 宁波市大数据投资发展有限公司浙江宁波315000

为了探究不同支流洪水组合对干流区域的致灾影响,构建了一二维耦合水动力模型并进行验证.采用耦合模型分别对理想Y型流域和宁波市海曙区三江口区域进行建模,模拟了不同峰现时差的支流洪水过程,选取了干流洪峰流量、积水量、淹没面积、... 详细信息

为了探究不同支流洪水组合对干流区域的致灾影响,构建了一二维耦合水动力模型并进行验证.采用耦合模型分别对理想Y型流域和宁波市海曙区三江口区域进行建模,模拟了不同峰现时差的支流洪水过程,选取了干流洪峰流量、积水量、淹没面积、最大淹没水深和最大漫出距离5个指标来反映洪水在干流区域的致灾情况.研究结果表明:(1)所构建的一二维耦合模型可以较好地反映一维河道和二维地表的水量交换过程;(2)在理想算例中,随着支流洪水峰现时差的增加,洪水对干流区域的致灾情况明显减轻,所选取的5个指标分别削减了1.52%~22.34%、5.70%~75.48%、8.49%~97.60%、1.88%~36.45%和2.03%~37.56%;(3)随着余姚江和奉化江洪水峰现时差的增加,甬江区域受洪水的影响明显下降,所选取的5个指标分别削减了10.03%~21.47%、52.28%~94.16%、56.05%~97.55%、11.69%~28.46%和29.21%~76.64%.以期该研究为平原河网区域的防洪工作开展提供参考.

关键词：水动力模型一二维耦合模型城市内河洪水平原河网区域洪水组合洪水峰现时差