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miR-924、SLC1A5在肺癌组织中的表达及其在预后评估中的价值

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检验医学 2024年第1期39卷 13-18页

作者：张苗苗段东奎余春王丽娜南阳市中心医院胸外科肺部病区河南南阳473000

目的探讨miR-924和溶质载体家族1成员5(SLC1A5)在肺癌患者预后评估中的价值。方法选取2018年2月—2019年2月南阳市中心医院肺癌患者97例,收集所有患者的临床资料,并收集癌组织和癌旁组织(距肿瘤边缘>2 cm),检测miR-924、SLC1A5 mRNA和... 详细信息

目的探讨miR-924和溶质载体家族1成员5(SLC1A5)在肺癌患者预后评估中的价值。方法选取2018年2月—2019年2月南阳市中心医院肺癌患者97例,收集所有患者的临床资料,并收集癌组织和癌旁组织(距肿瘤边缘>2 cm),检测miR-924、SLC1A5 mRNA和SLC1A5蛋白表达。采用Pearson相关分析评估miR-924与SLC1A5 mRNA的相关性。采用Kaplan-Meier生存曲线评估肺癌患者的生存情况。采用Cox回归分析评估肺癌患者预后不良的危险因素。结果与癌旁组织比较,癌组织miR-924相对表达量降低(P<0.001),SLC1A5mRNA相对表达量升高(P<0.001)。癌组织SLC1A5蛋白阳性率显著高于癌旁组织(P<0.001)。癌组织miR-924表达与SLC1A5 mRNA表达呈负相关(r=-0.843,P<0.05)。根据癌组织miR-924相对表达量均值或SLC1A5蛋白的表达情况分别分为高表达组、低表达组和阳性组、阴性组。miR-924低表达组与高表达组之间、SLC1A5阳性组与阴性组之间分化程度、临床分期差异均有统计学意义(P<0.05)。Kaplan-Meier生存曲线分析结果显示,miR-924高表达组累积生存率高于低表达组(Log-rankχ^(2)=5.453,P<0.05);SLC1A5阳性组累积生存率低于阴性组(Log-rankχ^(2)=9.259,P<0.05)。多因素Cox回归分析结果显示,临床分期Ⅲ期、miR-924低表达、SLC1A5蛋白表达阳性均是肺癌患者预后不良的危险因素(P<0.05)。结论肺癌患者癌组织mi R-924和SLC1A5均呈异常表达,或可作为肺癌预后评估的生物标志物。

关键词：微小RNA-924 溶质载体家族1成员5 肺癌预后

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沃柑混倍体新种质的鉴定及评价

果树学报 2024年第7期41卷 1265-1274页

作者：赵洪涛陆珍李果果解凯东郑吉祥梁增刘要鑫欧智涛梁春王举兵周双云陈东奎陈香玲广西壮族自治区农业科学院园艺研究所南宁530007 华中农业大学园艺林学学院武汉430070 广西壮族自治区水果技术指导站南宁530022 广西桂洁农业开发有限公司南宁530041

【目的】鉴定和评价沃柑变异材料,为利用提供依据。【方法】采用流式细胞仪、石蜡切片和扫描电镜方法进行倍性和叶片显微结构鉴定,比较形态特征和果实品质。【结果】变异材料为二、四混倍体(2x+4x),倍性细胞比例为2x∶4x=39.1∶60.9;混... 详细信息

【目的】鉴定和评价沃柑变异材料,为利用提供依据。【方法】采用流式细胞仪、石蜡切片和扫描电镜方法进行倍性和叶片显微结构鉴定,比较形态特征和果实品质。【结果】变异材料为二、四混倍体(2x+4x),倍性细胞比例为2x∶4x=39.1∶60.9;混倍体的叶片长、宽、周长和面积比二倍体显著增加14.75%、31.01%、19.38%和46.09%,叶形指数显著减小12.61%;两者均为两性花,花瓣数、花瓣颜色、花丝离合状态和花柱状态相同,花瓣长度和宽度均显著大于二倍体;种子均为单胚类型,单粒质量、横径和纵径较二倍体分别显著增加30.77%、8.12%和26.64%;单果质量、纵径、横径、果形指数、果皮厚度和果实硬度比二倍体分别显著增加29.67%、13.38%、8.57%、3.85%、38.31%和16.37%,单果种子数显著减少61.68%;可溶性固形物和可滴定酸含量无显著差异,可食率显著降低,出汁率高,维生素C含量显著增加39.70%;叶片、下表皮、栅栏组织和海绵组织厚度较二倍体分别显著增加19.75%、10.10%、9.38%和43.92%,气孔长度和密度分别显著减小7.36%和15.76%,气孔宽度显著增加5.09%。【结论】鉴定获得了沃柑二、四混倍体新种质,与二倍体在形态特征、果实品质及叶片显微结构等方面存在较大差异。

关键词：沃柑二倍体混倍体形态特征果实品质显微结构

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All-nanofiber self-powered PTFE/PA66 device for real-time breathing monitor by scalable solution blow spinning technology

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Nano Research 2022年第9期15卷 8458-8464页

作者： Pan Li Yibo Liu Han Zhang Zhiping Hu Luna Jia dongkui Liu Lu Yu Bo Li Youwei Yao School of Materials Science and Engineering Tsinghua UniversityBeijing 100084China Institute of Materials Research Tsinghua Shenzhen International Graduate SchoolShenzhen 518055China

All-nanofiber self-powered device was fabricated using simple,low-cost,safe,and scalable solution blow spinning(SBS)technology for real-time respiratory monitor and timely identification of respiratory obstruction ***(PTFE)and polyamide-66(PA66)nanofibers were selected as triboelectric pairs,owing to strong ability to gain electrons of PTFE and supply electrons of ***(ethylene oxide)(PEO)was added to regulate spinning solution viscosity and prepare PTFE/PEO nanofibers,and the morphology and diameter distribution of nanofibers were *** nanofiber film was obtained after the decomposition of PEO in PTFE/PEO nanofiber and melt flow of PTFE pellets in a limited region,and possessed a tensile strength of 1.05 MPa and elongation at a break of 288.58%.Later,PTFE/PA66 all-nanofiber self-powered device was constructed containing PA66 nanofibers,and Au deposition film was used as electrodes by magnetron *** as-obtained device showed robust electrical performance with an open circuit voltage of~110 V at a loading force of 10 N,a short-circuit current of~5 uA at a loading force of 10 N and a frequency of 4 Hz,a maximum power density of 562 mW·m^(–2),and a current of 3.1 uA at a loading resistance of 30 MΩ.Based on the triboelectric mechanism,the device possessed stable response and effective sensibility for stimuli,was used to monitor human breathing conditions,prevent suffocation,and distinguish slow,normal,and fast breathing,with an output voltage of~0.08 V perceived in one normal respiratory circle.

关键词： polytetrafluoroethylene(PTFE) nanofibers triboelectric flexible electronics

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叙事医学平行病历在外科实习教学中的应用

继续医学教育 2024年第2期38卷 81-84页

作者：吴铁军张宁宋东奎徐玉生郑州大学第一附属医院外科学教研室河南郑州450052

目的探讨叙事医学平行病历书写在医学生外科学实践教学中的应用。方法将2021年7月至2022年6月郑州大学第一临床学院临床医学本科(5+3一体化)180名学生根据随机原则分成叙事医学平行病历教学组和传统教学组,叙事医学平行病历教学组在外... 详细信息

目的探讨叙事医学平行病历书写在医学生外科学实践教学中的应用。方法将2021年7月至2022年6月郑州大学第一临床学院临床医学本科(5+3一体化)180名学生根据随机原则分成叙事医学平行病历教学组和传统教学组,叙事医学平行病历教学组在外科临床实习期间每4周书写1份平行病历,传统教学组以传统教学方法完成实习教学计划,实习结束后测量医患沟通能力、共情能力,问卷调查统计带教老师评价指标、患者满意度。结果实习结束时叙事医学平行病历教学组医患沟通能力评分高于传统教学组[(87.21±5.13)分vs.(73.32±4.37)分],杰弗逊共情量表评分亦高于传统教学组[(103.42±4.48)分vs.(78.32±7.39)分],带教老师对实习医学生工作热情、疾病掌握程度的评价得分分别为(93.85±7.38)分vs.(85.27±5.44)分、(78.51±6.53)分vs.(63.33±7.72)分,均差异有统计学意义(P<0.001)。2组问卷调查患者满意度分别为82.3%、67.8%,差异有统计学意义(P<0.05)。结论将叙事医学平行病历应用在外科实习教学中,能够促进医学生共情能力、医患沟通能力培养,提高岗位胜任力和医学人文素质。

关键词：叙事医学平行病历实习教学医患沟通医学生培养医学人文

Neurofilament 200 expression in a rat model of complete spinal cord injury following growth-associated protein-43 treatment

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Neural Regeneration Research 2009年第11期4卷 827-831页

作者： Yanping Duan dongkui Zhang Yingchun Ba Yun Yuan Jun Sun Dengli Fu Ran Zhang Jinde Wang Department of Histology and Embryology Kunming Medical University Kunming 650031 Yunnan Province China Yunnan Medical College Kunming Medical University Kunming 650031 Yunnan Province China Department of Anatomy Kunming Medical University Kunming 650031 Yunnan Province China Second Affiliated Hospital Kunming Medical University Kunming 650031 Yunnan Province China

BACKGROUND： Growth-associated protein-43 （GAP-43） expression in the nervous system has been demonstrated to promote neural regeneration, neuronal growth and development, as well as synaptic reconstruction. Neurofilament 200 （NF200） expression could reflect degree of injury and repair in injured spinal axons. OBJECTIVE： To observe NF200 expression changes in a rat model of complete spinal cord injury following GAP-43 treatment and to explore the effects of GAP-43 following spinal cord injury. DESIGN, TIME AND SETTING： A randomized, controlled, animal experiment was performed at the Laboratory of Histology and Embryology of Kunming Medical University between March 2007 and October 2008. MATERIALS： GAP-43 and GAP-43 antibody were provided by Beijing Boao Biology, China; mouse anti-rat NF200 antibody was purchased from Chemicon, USA. METHODS： Female, 8-week-old, Sprague Dawley rats were randomly assigned into three groups following complete spinal cord injury, with 20 animals in each group： GAP-43 antibody, GAP-43, and model groups. In addition, each group was subdivided into four subgroups according to sampling time after modeling, Le., 3-, 5-, 9-, and 15-day groups, with 5 rats in each group. GAP-43 antibody or GAP-43 was injected into injury sites of the spinal cord, 5 μg/0.2 mL, respectively, twice daily for three consecutive days, followed by three additional days of injection, once daily. The model group did not receive any treatment following injury. MAIN OUTCOME MEASURES： NF200 expression in the damaged spinal area at different stages was detected by immunohistochemistry; lower limb motion function following injury was evaluated using the Basso, Beattie and Bresnahan （BBB） locomotor rating scale. RESULTS： NF200 expression was significantly reduced in the GAP-43 antibody group, compared with GAP-43 and model groups, at 3 and 5 days after spinal cord injury （P 〈 0.05）. In addition, the model group expressed significantly less NF200 than the GAP-43 group （P 〈 0.05）

关键词： spinal cord injury growth-associated protein-43 neurofilament 200 Basso, Beattie and Bresnahan locomotor rating scale

基于定性网络模型评价生态调控情景对海洋牧场生态系统的影响——以獐子岛海洋牧场为例

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生态学报 2024年第13期44卷 5761-5772页

作者：孙国庆张合烨董世淇李磊王兆国孙旭李明高东奎田涛吴忠鑫大连海洋大学水产与生命学院大连116023 辽宁省海洋牧场工程技术研究中心大连116023 大连海洋大学设施渔业教育部重点实验室大连116023 獐子岛集团股份有限公司大连116001

在海洋牧场建设过程中,生态调控方式实施后对海洋牧场生态系统的影响通常难以预测,这对海洋牧场的生态安全和高质量发展提出了严峻挑战。为此,建立了一种基于定性网络模型(Qualitative network model,QNM)的海洋牧场生态系统模拟评价方... 详细信息

在海洋牧场建设过程中,生态调控方式实施后对海洋牧场生态系统的影响通常难以预测,这对海洋牧场的生态安全和高质量发展提出了严峻挑战。为此,建立了一种基于定性网络模型(Qualitative network model,QNM)的海洋牧场生态系统模拟评价方法,并以獐子岛海洋牧场近岸增殖海域作为研究区域,构建以增殖目标种为核心的定性网络模型,模拟评估海洋牧场3种不同类型的生态调控情景(增殖目标种、移除捕食者、海藻场修复)及其复合条件下,牧场群落范围内的响应,分析海洋牧场生态调控策略与生物功能群变化之间潜在关系。结果显示:目标种增殖(仿刺参和虾夷扇贝)产生的上行效应导致其捕食者呈现积极响应,产生的下行效应导致其它底栖动物、浮游植物和有机碎屑等功能群呈现消极响应,移除捕食者海星产生的下行效应导致虾夷扇贝呈现积极响应,表明在增殖区清除敌害生物的重要性,海藻场修复对整个群落有明显的积极影响,体现了海藻场在养护近岸生态系统的重要生态意义。研究表明:QNM可有效识别生态系统潜在的营养级联效应,评估生物功能群的响应,基于QNM的海洋牧场生态调控模拟评价方法,突破了定量食物网模型在数据有限系统中使用的局限性,可为海洋牧场建设的生态调控策略制定提供科学参考。

关键词：海洋牧场定性网络模型相互作用增殖移除捕食者海藻场修复

基于贝叶斯优化BP神经网络的压力容器最小壁厚预测研究

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科学技术创新 2024年第22期 69-72页

作者：冯晓刚闫风影沈冬奎韩贯凯山东省安泰化工压力容器检验中心有限公司山东济南

压力容器最小壁厚对压力容器的结构强度和稳定性具有重要作用。鉴于此,利用贝叶斯优化方法结合BP神经网络,实现对压力容器最小壁厚的精确预测。首先,采用贝叶斯方法优化隐含层神经元数量,得到最优隐含层神经元数量。然后,使用该最优神... 详细信息

压力容器最小壁厚对压力容器的结构强度和稳定性具有重要作用。鉴于此,利用贝叶斯优化方法结合BP神经网络,实现对压力容器最小壁厚的精确预测。首先,采用贝叶斯方法优化隐含层神经元数量,得到最优隐含层神经元数量。然后,使用该最优神经元数进行模型训练。最后,将验证集输入到模型中进行验证,以得到压力容器最小壁厚的预测结果。结果表明,基于贝叶斯方法优化BP神经网络的模型能够精确地预测压力容器筒体和封头的最小壁厚,且均方误差仅为0.2678和0.7448,该模型能够提供更可靠的设计和决策依据,为提高压力容器结构设计的效率和安全性做出重要贡献。

关键词：压力容器壁厚贝叶斯优化 BP 预测

Gastrointestinal Stromal Tumors in the Rectum

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Chinese Journal of Clinical Oncology 2005年第6期2卷 881-887页

作者： Hongwei Lin Yongfu Shao dongkui Xu Dongbing Zhao Haizeng Zhang Tiecheng Wu Department of Abdominal Surgery Cancer Hospital Peking Union MedicalCollege Chinese Academy of MedicalScience Beijing 100021 China.

OBJECTIVE To investigate the clinical and pathologic features of rectal gastrointestinal stromal tumors （GIST） and to evaluate their reasonable management. METHODS The clinical and pathological data for 19 patients with rectal GIST over the past 19 years were studied retrospectively. RESULTS The diagnosis of the 19 cases was identified by surgery and pathology. All the rectal GISTs were spindle cell type with immunohistochemical analysis showing positive reactivity for CD117 （100%） and CD34 （73.7%）. There were 4 cases of high risk, 3 cases of intermediate risk, 5 cases of low risk and 7 cases of very low risk of aggressive behavior in this study. CONCLUSION Rectal GIST, without specific symptoms in the early stage, has a low incidence and usually shows low risk of aggressive behavior. It is difficult to produce an accurate pathological diagnosis before operation and it is difficult to decide whether to save the sphincter before or during operation. Reasonable initial treatment includes trans-anal local resection as the best recommend management of low risk submucosal rectal GIST （〈3.0 cm）.

关键词： clinical pathology immunohistochemislry neoplasma of the rectum.

Estimation on Flux and Power Consumption of Twin Screw Pulping Extruder Based on Dimensional Analysis

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Estimation on Flux and Power Consumption of Twin Screw Pulpi...