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小地老虎2种不同类型肌动蛋白基因的克隆与序列分析

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植物保护 2008年第6期34卷 12-19页

作者：朴冬花姚磊贺玉年樊东东北农业大学农学院哈尔滨150030

本研究以鳞翅目夜蛾科昆虫小地老虎[Agrotis ipsilon(Hfnagel)]3龄幼虫整个虫体为材料提取总RNA,利用RT-PCR和cDNA末端快速扩增技术(RACE),分别扩增得到2条肌动蛋白的cDNA序列Aiactin1和Aiactin2。GenBank数据库搜索及序列比对结果表... 详细信息

本研究以鳞翅目夜蛾科昆虫小地老虎[Agrotis ipsilon(Hfnagel)]3龄幼虫整个虫体为材料提取总RNA,利用RT-PCR和cDNA末端快速扩增技术(RACE),分别扩增得到2条肌动蛋白的cDNA序列Aiactin1和Aiactin2。GenBank数据库搜索及序列比对结果表明,克隆的2条肌动蛋白基因应属于2种不同类型的肌动蛋白,Aiactin1为肌肉特异型肌动蛋白,Aiactin2为细胞质特异型肌动蛋白。Aiactin1的cDNA序列含有1469个碱基,Aiactin2的cDNA序列含有1408个碱基。2条基因的cDNA序列均包括一个1131个碱基的开放阅读框,编码一个含376个氨基酸的蛋白。Aiactin1肌动蛋白分子量约为41.77ku,等电点5.22;Aiactin2肌动蛋白分子量约为41.83ku,等电点5.47。Prosite软件分析结果表明,Aiactin1和Aiactin2肌动蛋白氨基酸序列中都存在3个肌动蛋白特征片段。2个基因的cDNA序列已经登录GenBank并获得登录号。

关键词：小地老虎肌动蛋白克隆序列分析

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组合梁挠度计算的新方法——有效刚度法

西南交通大学学报 2011年第4期46卷 541-546页

作者：周东华孙丽莉樊江赵志曼刘永芳昆明理工大学建筑工程学院云南昆明650224

要精确计算弹性剪切连接时组合梁的挠度较为复杂,因而很难得到任意荷载作用下的解析解,一些常用荷载作用下的解析解冗长,计算繁琐.为此,GB 50017—2003《钢结构规范》中提供了一种较为简便的计算方法——抗弯刚度折减法,但用该方法计算... 详细信息

要精确计算弹性剪切连接时组合梁的挠度较为复杂,因而很难得到任意荷载作用下的解析解,一些常用荷载作用下的解析解冗长,计算繁琐.为此,GB 50017—2003《钢结构规范》中提供了一种较为简便的计算方法——抗弯刚度折减法,但用该方法计算时,精度不高,适用范围有局限.为此,提出了组合梁挠度计算的一种新方法——有效刚度法.该方法计算简便、力学概念清晰,且计算精度很高,与精确解析解的误差不超过1.0%;此外,还能给出组合系数的值,能非常直观地评价组合梁组合作用的大小;该方法对剪切连接件的刚度无任何限制,其变化范围可以从趋近于0到无穷大.

关键词：有效刚度法组合梁挠度弹性剪切连接组合系数

Pearling and helical nanostructures of model protocell membranes

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Nano Research 2022年第1期15卷 659-668页

作者： Zhidi Chen Yaxun fan Yao Chen Jeffrey Penfold Peixun Li Rongliang Wu Yilin Wang CAS Key Laboratory of Colloid Interface and Chemical ThermodynamicsBeijing National Laboratory for Molecular Sciences(BNLMS)CAS Research/Education Center for Excellence in Molecular SciencesInstitute of ChemistryChinese Academy of SciencesBeijing 100190China University of Chinese Academy of Sciences Beijing 100049China ISIS STFCy Rutherford Appleton LaboratoryChiltonDidcotOxon OX110QXUK College of Material Science and Engineering State Key Laboratory for Modification of Chemical Fibers and Polymer MaterialsDonghua UniversityShanghai 201620China

The diversity of protocell membrane structures is crucial for the regulation of cell activities and indispensable to the origin of *** to the evolution of complex cellular machinery,spontaneous protocell membrane evolution results from the intrinsic physicochemical properties of simple molecules under specific environmental ***,we report the evolution of the morphology of cell-sized model protocell membranes from giant vesicles to pearling and helical nanostructures,resembling morphologies of eukaryocytes,nostoc,and *** evolution occurs in a single binary aqueous system composed of an achiral single-chain amphiphile and a biogenic polyamine(spermidine or spermine)upon evaporating water,feeding amphiphiles,or increasing pH in response to various primitive fluctuating *** contrast,nonbiogenic polyamines(triamine,triethylenetetramine,and hexamethyltriethylenetetramine)with slight differences in the number of methylene groups or protonated amine groups do not induce such a kind of *** evolution of the shape transformation strongly relies on the balance between electrostatic attraction and hydrogen bonding,attributed to the odd/even effect of polyamines in the ***,both pearling and helical structures emerge from multilamellar vesicles undergoing different processes,where the helix shows stronger permeability and encapsulation capability due to its multicompartmentalized ***,subtle adjustment of weak intramolecular interactions not only yields significant changes in the morphological evolution of protocell membranes but also brings new insights into the natural inevitability of biogenic small molecules.

关键词： protocell membrane pearling and helical nanostructures morphology evolution biogenic polyamine amphiphile self-assembly

不同肝细胞配体修饰的壳聚糖作为肝组织再生支架材料的比较研究:制备、表征和评价

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Journal of donghua University(English Edition) 2024年第3期41卷 263-274页

作者：王哲余凡姜玉新侯玉婷帅兰邓敏王红声东华大学生物与医学工程学院上海纳米生物材料与再生医学工程技术研究中心上海201620 嘉兴大学附属嘉兴市第一医院嘉兴市病毒相关传染性病重点实验室浙江嘉兴314001

为了获得理想的肝脏再生支架,使用乳酸(lactobionic acid,LA)或/和甘草次酸(glycyrrhetinic acid,GA)对壳聚糖(chitosan,CS)进行改性,得到LA改性的CS(LC)、GA改性的CS(GC)和GA/LA双改性的CS(GLC),并采用绿色静电纺丝法制备了由丝素蛋白(silk fibroin,SF)和上述改性CS组成的复合纳米纤维支架。这些支架是亲水的,其亲水性和热稳定性随着改性CS的增加而降低,而结晶度则相反。这些支架在促进HepG2细胞增殖及分泌白蛋白和尿素方面表现出良好的性能。此外,与SF/GC或SF/GLC支架相比,SF/LC支架具有更好的肝细胞相容性。

关键词：甘草次酸(GA) 半乳糖壳聚糖(CS) 丝素蛋白(SF) 肝组织工程

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Ag掺杂多晶SnSe基热电材料的性能研究

现代技术陶瓷 2024年第1期45卷 177-187页

作者：鲁文彬罗维范宇驰丁奇江莞东华大学材料科学与工程学院上海201620 东华大学功能材料研究中心上海201620

近年来,由于环境污染与能源危机的日益严重,使得国内外学者对可再生能源的研究越发重视,热电材料因其能够实现电与热的直接转换而引起了广泛关注。但热电器件的转换效率较低限制了其实际应用,为了提高其转换效率,大部分研究工作都聚焦... 详细信息

近年来,由于环境污染与能源危机的日益严重,使得国内外学者对可再生能源的研究越发重视,热电材料因其能够实现电与热的直接转换而引起了广泛关注。但热电器件的转换效率较低限制了其实际应用,为了提高其转换效率,大部分研究工作都聚焦于提高热电材料的热电优值(ZT)上。SnSe是目前性能优良的中温热电材料,本文通过水热法和在低于传统烧结温度的条件下成功制备了Ag掺杂的多晶AgxSn_(1-x)Se(x=0.0025、0.005、0.0075)热电材料。结果表明,Ag的掺入明显改善了材料的载流子浓度,同时样品保持了较高的Seebeck系数,使得材料的电学性能显著提高。当x=0.0075时,Ag_(0.0075)Sn_(0.9925)Se具有相对较高的ZT值,800K时可达1.2,但是对该样品进行退火处理后ZT值有所降低,800 K时约为0.8。

关键词： SnSe Ag掺杂热电材料 ZT值

追施钾肥对‘重阳红’桃果实产量和品质及采果前树体营养的影响

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西北农业学报 2016年第5期25卷 730-737页

作者：白红陶佳石佩郭东花范崇辉西北农林科技大学园艺学院陕西杨凌712100

以盛果期10a生中晚熟品种‘重阳红’桃为试材,在大田条件下,以不追施钾肥（K0）为对照,设置单株施钾肥（K2SO4）量0.47kg（K1）、0.70kg（K2）和0.93kg（K3）3种处理进行生育期试验。研究在膨果期追施不同水平钾肥对果实产量和品质及采... 详细信息

以盛果期10a生中晚熟品种‘重阳红’桃为试材,在大田条件下,以不追施钾肥（K0）为对照,设置单株施钾肥（K2SO4）量0.47kg（K1）、0.70kg（K2）和0.93kg（K3）3种处理进行生育期试验。研究在膨果期追施不同水平钾肥对果实产量和品质及采果前树体营养的影响。结果表明：与对照相比,在‘重阳红’桃膨果期增施钾肥可增产10.58%-23.58%,K2处理产量最高,其增产效应符合＂报酬递减＂规律;增施钾肥可不同程度提高桃果实内在品质,其中K2处理可显著增加果实中的可溶性固形物、可溶性淀粉、N及K的质量分数,且分别为对照的1.14、1.78、1.22和2.18倍;增施钾肥还可促进果实成熟前树体营养的积累,K2处理叶片的净光合速率（Pn）、蒸腾速率（Tr）、气孔导度（Gs）和氮、可溶性淀粉、还原性糖、可溶性糖的质量分数均显著或极显著高于对照,且分别为对照的1.37、1.45、1.84、1.34、1.46、1.19和1.21倍,枝条的N和P质量分数分别是对照的1.43和1.50倍,而其他处理间无显著差异。可见,膨果期追施钾肥可提高桃的产量和果实品质,也可促进采果前树体营养的积累,且以单株施钾肥（K2SO4）0.70kg的效果最佳。

关键词：桃树钾肥产量品质营养

Silk materials for medical, electronic and optical applications

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Science China(Technological Sciences) 2019年第6期62卷 903-918页

作者： fan SuNa ZHANG Yi HUANG XiangYu GENG LiHong SHAO HuiLi HU XueChao ZHANG YaoPeng State Key Laboratory for Modification of Chemical Fibers and Polymer Materials International Joint Laboratory for Advanced Fiber and Low-Dimension Materials College of Materials Science and Engineering Donghua University Shanghai 201620 China Key Laboratory of Polymer Materials and Products of Universities in Fujian Fujian University of Technology Fuzhou 350118 China

Silk derived from the silkworm Bombyx mori is among the most important fibrous protein biomaterials due to large-scale production from natural sources, excellent biocompatibility, unique mechanical properties and controllable degradation. Silk fibroin can be processed into a variety of formats to match different applications, such as tissue engineering, drug delivery or as the passive substrate of a bio-device. Advances in fabrication technologies provide new possibilities for the combination of silk fibroin with other nanomaterials to functionalize silk fibroin for specialized purposes, including sensing, cell visualization,resistance to ultraviolet light and provision of antibacterial properties. As the requirement for wearable and intelligent devices has become increasingly important over recent years, silk fibroin has been utilized as the active element in electronic and optical instruments. This review summarizes these recent advances in the innovative applications of silk fibroin.

关键词： silk fibroin tissue engineering drug delivery electronic devices sensor optics

Porcine alpha interferon inhibit Japanese encephalitis virus replication by different ISGs in vitro

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Porcine alpha interferon inhibit Japanese encephalitis virus...

In-situ growth of carbon nanotubes on ZnO to enhance thermoelectric and mechanical properties

第四届全国人畜共患病学术研讨会 2014年中国狂犬病年会中国畜牧兽医学会兽医公共卫生学分会第四次学术研讨会

作者： Ke Liu donghua Shao Jianchao Wei Beibei Li Xuewen Liao Bin Zhou Huijuan Yao Shengchao fan Puyan Chen Denian Miao Zhiyong Ma Shanghai Veterinary Research Institute Chinese Academy of Agricultural Science Key Laboratory of Animal Disease Diagnosis and Immunology College of Veterinary Medicine Nanjing Agricultural University Institute of Animal Husbandry and Veterinary Science Shanghai Academy of Agricultural Sciences

Japanese encephalitis virus(JEV) causes serious zoonosis in South Asia, Southeast Asia and other areas. Pigs are an important reservoir for this virus in nature. The treatment of JEV infection in pigs is important for controlling the prevalence of JEV in humans and economic losses in pig farming. In this study, we selected a high activity porcine alpha-interferon to inhibit JEV replication in porcine kidney cell lines(PK-15). Alpha interferon exhibited high antiviral activity against JEV;the expression of three inter-feron-stimulated genes(ISGs), ISG15, Mx2 and OAS L,increased significantly after interferon treatment. Furthermore, we verified the anti-JEV effect of these ISGs by RNAi and overexpression. Mx2 and OAS L exhibited strong anti-JEV effects in PK-15 cells. Based on these novel results, alpha interferon(IFN-a) should be considered to be a potential drug against JEV in pigs.

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Journal of Advanced Ceramics 2022年第12期11卷 1932-1943页

作者： Shengjie fan Tingting SUN Meng JIANG Shijia GU Lianjun WANG Haixue YAN Wan JIANG State Key Laboratory for Modification of Chemical Fibers and Polymer Materials College of Materials Science and EngineeringDonghua UniversityShanghai 201620China Engineering Research Center of Advanced Glasses Manufacturing Technology Ministry of EducationDonghua UniversityShanghai 201620China Institute of Functional Materials Donghua UniversityShanghai 201620China School of Engineering and Materials Science Queen Mary University of LondonLondon E14NSUK

As a high-temperature thermoelectric(TE)material,ZnO offers advantages of non-toxicity,chemical stability,and oxidation resistance,and shows considerable promise as a true ready-to-use module under air ***,poor electrical conductivity and high thermal conductivity severely hinder its *** nanotubes(CNTs)are often used as a reinforcing phase in composites,but it is difficult to achieve uniform dispersion of CNTs due to van der Waals ***,we developed an effective in-situ growth strategy of homogeneous CNTs on ZnO nanoparticles by exploiting the chemical vapor deposition(CVD)technology,in order to improve their electrical conductivity and mechanical properties,as well as reducing the thermal ***,magnetic nickel(Ni)nanoparticles are introduced as catalysts for promoting the formation of CNTs,which can also enhance the electrical and thermal transportation of ZnO ***,the electrical conductivity of ZnO is significantly boosted from 26 to 79 S·cm^(−1) due to the formation of dense and uniform conductive CNT *** lattice thermal conductivity(κ_(L))is obviously declined by the intensification of phonon scattering,resulting from the abundant grain boundaries and interfaces in ZnO-CNT ***,the maximum dimensionless figure of merit(zT)of 0.04 at 800 K is obtained in 2.0%Ni-CNTs/ZnO,which is three times larger than that of CNTs/ZnO prepared by traditional ultrasonic *** addition,the mechanical properties of composites including Vickers hardness(HV)and fracture toughness(K_(IC))are also *** work provides a valuable reference for dispersing nano-phases in TE materials to enhance both TE and mechanical properties.

关键词： ZnO carbon nanotubes(CNTs) in-situ grown thermoelectric(TE) mechanical properties