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Inhibition of the Growth of Raji Cells by Precursor MicroRNA-15a

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Clinical oncology and cancer researeh 2010年第1期7卷 22-26页

作者： dong-mei HE qin chen Institute of Hematology Medical College of Jinan University Guangzhou 510632 Guangdong Province China.

OBJECTIVE To investigate the effects of microRNA-15a （miR- 15a） on the growth of the lymphoma Raji cell line. METHODS A eukaryotic expression vector of precursor mLR-15a （pre-miR-15a） was constructed. Paired oligonucleotides for pre- miR-15a expression were designed and synthesized chemically. Annealed oligonucleotides were inserted into a pGCSIL- GFP vector under a U6 promoter to construct a pre-miR-15a expression plasmid. Oligonucleotides with a scrambled sequence which were used as a negative control were also constructed into the pGCSIL-GFP vector. A recombinant expression vector was identified through PCR and sequencing. A pre-miR-15a expression plasmid and a negative control plasmid were all transferred into Raji cells with lipofectamine 2000. Quantitative reverse-transcription polymerase chain reaction （qRT-PCR） was employed to explore expression of miR-15a. The expression levels of the protein were assayed via immunofluorescence. The growth effect of Raji cells was measured by CCK8 assay. Apoptosis was determined using Hoechst dyeing and flow cytometry. The growth-inhibitory effect on Raji cells was measured using a CCK8 assay. RESULTS The results showed that the insertion sequence was correct. The expression level of miR-15a was obviously higher in Raji cells transferred with pre-miR-15a plasmid than in the blank group and in the negative control group. After Raji cells were transferred with the pre-miR-15a expression plasmid for 48 h, Bcl-2 protein expression levels were obviously decreased, which indicated that there was a significant difference as compared with the negative control group and blank group （P 〈 0.05）. The CCK8 assay showed that transfection of pre-miR-15a expression plasmid decreased the cell growth at 24, 48 and 72 h post-transfection. After Raji cells were transferred with pre-miR-15a expression plasmid, apoptotic cells can be seen with Hoechst dyeing, and the cell apoptotic rate was obviously higher than that in blank group and negative

关键词： hsa-mir-15 microRNA lymphoma cell line Bcl-2 protein.

EFFECT OF ADDITIVES ON Li-La-TiO2 CATALYST PROPERTIES IN METHANE OXIDATIVE COUPLING

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Journal of Energy Chemistry 1992年第3期 235-243页

作者： GONG Mao-Chu chen Yu XU Zhen-Hong ZHAN Wei-dong qin Dai-Yi chen Yao-Qiang ZHOU Jian-Lue PAN Gui-Huang Department of Chemistry,Sichuan University,chengdu,Sichuan 610064 Received December 21,1991

For adjusting the surface properties of the catalysts some metroxides as the promoter were added into Li-La/TiOsystem and enhanced theactivity and ethylene selectivity for the oxidative coupling of *** of the reactant composition,the gas hourly space velocity（GHSV）andthe reaction temperature on the performance of the catalysts were *** was found that the methane conversion,Cselectivity and Cyieid wereaffected by the *** SUC series exhibited high activity,selectivityand *** catalyst SUC-3,methane conersion was up to 41.5%,Cselectivity,61.7% and Cyield,25.6% at 770℃ and GHSV=***-2 has continuously been operated at 850C and GHSV=3428hfor 500 hours,the methane conversion,Cselectivity and Cyieldkept at the level of about 30,60 and 18% respectively.

关键词： EFFECT OF ADDITIVES ON Li-La-TiO2 CATALYST PROPERTIES IN METHANE OXIDATIVE COUPLING Li La TiO

Electrocatalytic cleavage of a lignin β-O-4 model compound and coupling with nitrogen-containing aromatics using Prussian blue analogue-derived nickel–cobalt spinel

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Journal of Energy Chemistry 2025年第1期100卷 628-636页

作者： Yi Qi Xueying chen Bowen Liu Xuliang Lin Xueqing Qiu Yanlin qin Guangdong Provincial Key Laboratory of Plant Resources Biorefinery School of Chemical Engineering and Light Industry Guangdong University of Technology Guangdong Provincial Laboratory of Chemistry and Fine Chemical Engineering Jieyang Center Guangdong Basic Research Center of Excellence for Ecological Security and Green Development Guangdong University of Technology

Electrochemical conversion of lignin for the production of high-value heterocyclic aromatic compounds has great *** demonstrate the targeted synthesis and cation modulation of NiCo2O4spinel nanoboxes,synthesized via cation exchange and calcination *** catalysts exhibit excellent efficacy in the electrocatalytic conversion of lignin model compounds,specifically 2-phenoxy-1-phenylethanol,into nitrogen-containing aromatics,achieving high conversion rates and *** catalysts were synthesized via a cation exchange and calcination oxidation process,using Prussian blue nanocubes as *** porous architecture and polymetallic composition of the NiCo2O4spinel demonstrated superior performance in electrocatalytic oxidative coupling,achieving a 99.2 wt%conversion rate of the 2-phenoxy-1-phenylethanol with selectivities of 37.5 wt%for quinoline derivatives and 31.5 wt%for *** innovations include the development of a sustainable one-pot synthesis method for quinoline derivatives,the elucidation of a multistage reaction pathway involving CAO bond cleavage,hydroxyaldol condensation,and CAN bond formation,and a deeper mechanistic understanding derived from DFT *** work establishes a new strategy for lignin valorization,offering a sustainable route to produce high-value nitrogen-containing aromatics from renewable biomass under mild conditions,without the need for additional reagents.

关键词： Spinel Biomass valorization Electrocatalysis Lignin oxidation Coupling

The emerging role of nitric oxide in the synaptic dysfunction of vascular dementia

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Neural Regeneration Research 2025年第2期20卷 402-415页

作者： Xiaorong Zhang Zhiying chen Yinyi Xiong qin Zhou Ling-Qiang Zhu Dan Liu Department of Pathology Affiliated Hospital of Jiujiang UniversityJiujiangJiangxi ProvinceChina Jiujiang Clinical Precision Medicine Research Center JiujiangJiangxi ProvinceChina Center for Cognitive Science and Transdisciplinary Studies Jiujiang UniversityJiangxi ProvinceChina Department of Neurology Affiliated Hospital of Jiujiang UniversityJiujiangJiangxi ProvinceChina Department of Rehabilitation Affiliated Hospital of Jiujiang UniversityJiujiangJiangxi ProvinceChina Department of Pathophysiology School of Basic MedicineTongji Medical CollegeHuazhong University of Science and TechnologyWuhanHubei ProvinceChina

With an increase in global aging,the number of people affected by cerebrovascular diseases is also increasing,and the incidence of vascular dementia-closely related to cerebrovascular risk-is increasing at an epidemic ***,few therapeutic options exist that can markedly improve the cognitive impairment and prognosis of vascular dementia *** in Alzheimer’s disease and other neurological disorders,synaptic dysfunction is recognized as the main reason for cognitive *** oxide is one of the ubiquitous gaseous cellular messengers involved in multiple physiological and pathological processes of the central nervous ***,nitric oxide has been implicated in regulating synaptic plasticity and plays an important role in the pathogenesis of vascular *** review introduces in detail the emerging role of nitric oxide in physiological and pathological states of vascular dementia and summarizes the diverse effects of nitric oxide on different aspects of synaptic dysfunction,neuroinflammation,oxidative stress,and blood-brain barrier dysfunction that underlie the progress of vascular ***,we propose that targeting the nitric oxide-sGC-cGMP pathway using certain specific approaches may provide a novel therapeutic strategy for vascular dementia.

关键词： endoplasmic reticulum stress endothelial nitric oxide synthase gene therapy nitric oxide NO-sGC-cGMP pathway synaptic dysfunction vascular dementia

Fine-tunable Organocatalysts Bearing Multiple Hydrogen Bonding Donors for Construction of Adjacent Quaternary and Tertiary Stereocenters via a Michael Reaction

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Fine-tunable Organocatalysts Bearing Multiple Hydrogen Bondi...

Low-temperature chlorination roasting technology for the simultaneous recovery of valuable metals from spent LiCoO_(2)cathode material

中国化学会第26届学术年会不对称催化分会场

作者： Chun-Jiang Wang, Zhi-Hai Zhang, Xiu-qin dong and dong chen College of Chemistry and Molecular Science, Wuhan University, Wuhan 430072

＜正＞The development of enantioselective catalytic protocols for Michael addition reaction has attracted much attention.[1] Of particular interest is the reaction between trisubstituted carbon nucleophiles and

关键词： Multiple Hydrogen Bonding Activation Bifunctional Catalyst Organocatalysis.

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International Journal of Minerals,Metallurgy and Materials 2025年第1期32卷 80-91页

作者： Junjie Shi Changle Hou Jingjing dong dong chen Jianzhong Li Key Laboratory for Ecological Metallurgy of Multimetallic Mineral(Ministry of Education) Northeastern UniversityShenyang 110819China School of Metallurgy Northeastern UniversityShenyang 110819China

With the continuous increase in the disposal volume of spent lithium-ion batteries(LIBs),properly recycling spent LIBs has become essential for the advancement of the circular *** study presents a systematic analysis of the chlorination roasting kinetics and proposes a new two-step chlorination roasting process that integrates thermodynamics for the recycling of LIB cathode *** activation energy for the chloride reaction was 88.41 kJ/mol according to thermogravimetric analysis–derivative thermogravimetry data obtained by using model-free,model-fitting,and Z(α)function(αis conversion rate).Results indicated that the reaction was dominated by the first-order(F1)model when the conversion rate was less than or equal to 0.5 and shifted to the second-order(F2)model when the conversion rate exceeded *** conditions were determined by thoroughly investigating the effects of roasting temperature,roasting time,and the mass ratio of NH_(4)Cl to LiCoO_(2).Under the optimal conditions,namely 400℃,20 min,and NH_(4)Cl/LiCoO_(2)mass ratio of 3:1,the leaching efficiency of Li and Co reached 99.43% and 99.05%,*** of the roasted products revealed that valuable metals in LiCoO_(2)transformed into CoCl_(2) and ***,the reaction mechanism was elucidated,providing insights for the establishment of a novel low-temperature chlorination roasting technology based on a crystal structure *** technology can guide the development of LIB recycling processes with low energy consumption,low secondary pollution,high recovery efficiency,and high added value.

关键词： spent lithium-ion battery thermodynamics chlorination roasting kinetics circular economy

Induced neural stem cells regulate microglial activation through Akt-mediated upregulation of CXCR4 and Crry in a mouse model of closed head injury

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Neural Regeneration Research 2025年第5期20卷 1416-1430页

作者： Mou Gao qin dong Dan Zou Zhijun Yang Lili Guo Ruxiang Xu Department of Neurosurgery Sichuan Academy of Medical Sciences and Sichuan Provincial People’s HospitalSchool of MedicineUniversity of Electronic Science and Technology of ChinaChengduSichuan ProvinceChina Department of Neurosurgery Chinese PLA General HospitalBeijingChina Department of Neurology Fu Xing HospitalCapital Medical UniversityBeijingChina

Microglial activation that occurs rapidly after closed head injury may play important and complex roles in neuroinflammation-associated neuronal damage and *** previously reported that induced neural stem cells can modulate the behavior of activated microglia via CXCL12/CXCR4 signaling,influencing their activation such that they can promote neurological ***,the mechanism of CXCR4 upregulation in induced neural stem cells remains *** this study,we found that nuclear factor-κB activation induced by closed head injury mouse serum in microglia promoted CXCL12 and tumor necrosis factor-αexpression but suppressed insulin-like growth factor-1 ***,recombinant complement receptor 2-conjugated Crry(CR2-Crry)reduced the effects of closed head injury mouse serum-induced nuclear factor-κB activation in microglia and the levels of activated microglia,CXCL12,and tumor necrosis factor-α.Additionally,we observed that,in response to stimulation(including stimulation by CXCL12 secreted by activated microglia),CXCR4 and Crry levels can be upregulated in induced neural stem cells via the interplay among CXCL12/CXCR4,Crry,and Akt signaling to modulate microglial *** agreement with these in vitro experimental results,we found that Akt activation enhanced the immunoregulatory effects of induced neural stem cell grafts on microglial activation,leading to the promotion of neurological recovery via insulin-like growth factor-1 secretion and the neuroprotective effects of induced neural stem cell grafts through CXCR4 and Crry upregulation in the injured cortices of closed head injury ***,these beneficial effects of Akt activation in induced neural stem cells were positively correlated with the therapeutic effects of induced neural stem cells on neuronal injury,cerebral edema,and neurological disorders post–closed head *** conclusion,our findings reveal that Akt activation may enhance the immunoregulatory effects of induced neura

关键词： Akt signaling cerebral edema closed head injury Crry CXCR4 induced neural stem cell microglia neuroinflammation

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电动汽车动力性能仿真分析

机械设计与制造 2012年第12期 114-116页

作者：王兴秦东晨裴东杰郑州大学机械工程学院郑州450001

为了使电动汽车满足动力需求,需要对其动力性能做深入的研究。以车辆系统动力学为理论基础,基于ADVISOR软件,建立了汽车部件和整车模型。选择道路循环工况,设置加速性能和爬坡性能的测试参数,运行仿真,并对仿真结果进行分析,分析结果表... 详细信息

为了使电动汽车满足动力需求,需要对其动力性能做深入的研究。以车辆系统动力学为理论基础,基于ADVISOR软件,建立了汽车部件和整车模型。选择道路循环工况,设置加速性能和爬坡性能的测试参数,运行仿真,并对仿真结果进行分析,分析结果表明电动汽车的动力性符合实际工作要求。在电动汽车设计和开发阶段,采用该研究方法得到的仿真结果,可以作为设计人员重要的参考依据。

关键词： ADVISOR 电动汽车动力性能仿真

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基于有限元的液压支架顶梁可靠性分析

煤矿机械 2010年第10期31卷 98-99页

作者：武红霞秦冬晨郑州大学机械工程学院郑州450001

采用APDL参数化语言创建顶梁的概率分析文件,把载荷、材料性能作为服从正态分布的随机变量,在Ansys的PDS模块中进行可靠性分析,得出最大变形和最大应力的累积分布函数及其可靠度,并分析出了影响其可靠度的主要输入参数。为液压支架的安... 详细信息

采用APDL参数化语言创建顶梁的概率分析文件,把载荷、材料性能作为服从正态分布的随机变量,在Ansys的PDS模块中进行可靠性分析,得出最大变形和最大应力的累积分布函数及其可靠度,并分析出了影响其可靠度的主要输入参数。为液压支架的安全可靠性生产提供了理论依据。

关键词：液压支架有限元可靠性分析